Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 229918435

GI number: 229918435

Start: 2693292

End: 2695934

Strand: Direct

Name: alaS [H]

Synonym: EAT1b_2722

Alternate gene names: 229918435

Gene position: 2693292-2695934 (Clockwise)

Preceding gene: 229918434

Following gene: 229918436

Centisome position: 89.78

GC content: 51.57

Gene sequence:

>2643_bases
ATGAAACCGCTCAAATCATTGACTGGCGCACAAATTCGCCAAATGTACTTAGATTTCTTCAAATCAAAAGGGCACAGCGT
GGAGCCGAGTGCTTCGCTCGTACCGGTCGAAGACCCAACTTTGTTGTGGATCAACTCTGGTGTTGCGACATTGAAAAAAT
ATTTTGATGGACGTGTCATCCCAGCGAACCCACGAATCGTCAACGCTCAAAAATCGATTCGTACGAACGATATCGAGAAC
GTCGGGAAGACAGCACGTCACCATACGTTCTTCGAGATGCTCGGGAACTTCTCAGTCGGTGAATACTTCCGTGAAGAAGC
GATCACATGGGGCTGGGAACTGTTGACGAGCGACGAATGGTACGGACTCGATCCGGACCGTCTCTCCGTCACGATTCACC
CGGACGATGAAGAATCAAAACAAATTTGGATGAACCTCGGCATGCCAGCTGACCGCATCATCCCGCTAGAAGATAACTTC
TGGGAAATCGGTGAAGGTCCATCTGGTCCAAACACGGAAATCTTCTTTGACCGTGGTCCAGCATTCGGGGACGACCCGAC
AGACCCTGAACTTTATCCGGGTGGGGAGAACGAGCGTTACCTTGAAATCTGGAACATCGTGTTCAGCCAATATAACCATG
ACGGACACGGAAACTACACAGAGCTTCCGCGTAAAAACATTGATACAGGGATGGGACTCGAACGGATGGCGAGCGTCCTT
CAAGACGTACCGACAAACTTCGATACCGACCTCTTCATGCCAATCATTCGTGAAACTGAAAAAATCAGTGGCGACGTATA
CCGTCAAGACAGCAGCAAAGACGTCGCGTTCAAAGTCATCGCGGACCACATTCGCACAGTATCGTTCGCAATCGGTGACG
GGGCACTCCCATCGAACGAAGGACGTGGCTATGTCCTCCGTCGTCTTCTCCGCCGTGCGGTCCGCTATGCGAAGATGCTT
GGCATCGAGCGTCCGTTCATGTATGAGCTCGTCGACGTCGTCGGTGACATCATGGTCGACTTCTACCCGCAAGTGAACGA
GAAGAAAGACTTTATCAAGAAAGTCATCAAAAACGAGGAAGAGCGTTTCCACGAAACGTTGAACGACGGACTCGCGATTT
TGAACGAAGTCGCAAAAGCACAAAAAGCAGCTGGGTCAAACATTATCAGCGGTCAAGACGCATTCCGTCTTTATGATACG
TACGGCTTCCCGCTCGAATTGACAATCGAATACGCAGAAGACCATCAGATGACGGTCGACGAGGACGGCTTCAAAGAAGC
GATGAACGAGCAACGTGAACGTGCACGTCAAGCGCGTGCTGACGTAGAATCGATGCAGATTCAGTCGGGTGTGCTTGCAG
ACATCACGATGCCATCGACATTCCTCGGATACAACAACCTTGAGACGAACGCAAAAATCATCAGCTTGATTCACAACGGC
GAACTCGCCGACGTCGTCGCTGCAGGAGAAGAGGCACAAGTCATCCTCGATCAGACGCCATTCTACGCCGAGAGCGGCGG
TCAAATCGGTGATAAAGGACAAATCGTCGGCGAAGACTTCCTTCTTCGCGTGAAAGATGTGTCGAAGGCACCGAACGGAC
AGAACTTGCATACGGTCACGGTCGAGTCAGGTGTGGCGAAAGCCGAAGTAGCCGTCACGGCTTCTGTCGACCAAGCATCG
CGTCAAGCCATCGTCAAGAACCACACGGCGACGCACCTCTTGCACCGCGCACTCAAAGATACGCTCGGGGAGCACGTCAA
CCAGGCTGGTTCACTCGTGACGCCAGACCGTCTTCGTTTCGACTTCTCTCACTTCGGTCAAGTGACATCAGAAGAGCTTC
AATCCATTGAGCGTCAAGTGAACGAGAAGATTTGGGCTTCAATCGGTGTCGATATTGAAGAGATGAACATTGCGGATGCG
AAGGCAAAAGGCGCAATGGCACTCTTCGGCGAGAAGTATGGTGATGTCGTTCGTGTCGTATCAGCAGGGGATTACTCGGT
TGAGCTTTGTGGTGGGATTCACGTCTCCAACACGGCTGAAATCGGACTCTTTAAAATCTTGTCTGAATCAGGGATTGGCG
CTGGGACACGTCGAATTGAAGCGGTCACGGGAGCTGGCGCATATGAAGTGATGAACCAGCAAATCGAAACGCTCGAAGCA
GCAGCGAAACTGTTGAAGACGAAACCACTTGAAGTTCCTGCACGCGTACAAGCACTTCAAGCTGAAATCAAAGAAGTCGA
GCGTGCTTCAGAGTCGCTCAAAGCGAAGCTTGCCAACATCGAGGCATCTTCATTAACGGATGCGATTGAAACGATTGGCG
GCGTTCAATTGATTGCGAAACGCGTCGATGGACAAGACATGGATGCCCTTCGTAGCATGGTCGACGACTTGAAAGGGAAA
CTCGGTTCTGCCGTGATTATCCTCGGGTCGGCAAATGGGGAGAAGGTCAACCTCGTCGCAGGTGTGACGAAAGACCTGAA
CGACCGTGGCTTCCATGCCGGAAAACTAATTAAAGAAGTCGCGACACGCTGCGGTGGTGGCGGTGGCGGTCGTCCAGACA
TGGCACAAGCAGGTGGCCGTGATGCAACAAAACTGGACGAAGCATTGAAGTTTGCTTCACATTACGTCGAATCACTGGCA
TAA

Upstream 100 bases:

>100_bases
TGCGAACGAGGGTGGTACCACGAAGCGATGCTTTCGTCCCTTTCCACAGGGGCAGGCATCGCTTTTTTTCGTTTCGTTCA
GAAATAAGGAGGAATTGTTC

Downstream 100 bases:

>100_bases
TAGAGTGTGAAGTGCAAACGGAAAGAGGTGGATTCTTGTGAGCCAAATGGATCGCACGATGCAATTCAATTTTCCAGAAG
ATGATCAACGTGCTAACACA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MKPLKSLTGAQIRQMYLDFFKSKGHSVEPSASLVPVEDPTLLWINSGVATLKKYFDGRVIPANPRIVNAQKSIRTNDIEN
VGKTARHHTFFEMLGNFSVGEYFREEAITWGWELLTSDEWYGLDPDRLSVTIHPDDEESKQIWMNLGMPADRIIPLEDNF
WEIGEGPSGPNTEIFFDRGPAFGDDPTDPELYPGGENERYLEIWNIVFSQYNHDGHGNYTELPRKNIDTGMGLERMASVL
QDVPTNFDTDLFMPIIRETEKISGDVYRQDSSKDVAFKVIADHIRTVSFAIGDGALPSNEGRGYVLRRLLRRAVRYAKML
GIERPFMYELVDVVGDIMVDFYPQVNEKKDFIKKVIKNEEERFHETLNDGLAILNEVAKAQKAAGSNIISGQDAFRLYDT
YGFPLELTIEYAEDHQMTVDEDGFKEAMNEQRERARQARADVESMQIQSGVLADITMPSTFLGYNNLETNAKIISLIHNG
ELADVVAAGEEAQVILDQTPFYAESGGQIGDKGQIVGEDFLLRVKDVSKAPNGQNLHTVTVESGVAKAEVAVTASVDQAS
RQAIVKNHTATHLLHRALKDTLGEHVNQAGSLVTPDRLRFDFSHFGQVTSEELQSIERQVNEKIWASIGVDIEEMNIADA
KAKGAMALFGEKYGDVVRVVSAGDYSVELCGGIHVSNTAEIGLFKILSESGIGAGTRRIEAVTGAGAYEVMNQQIETLEA
AAKLLKTKPLEVPARVQALQAEIKEVERASESLKAKLANIEASSLTDAIETIGGVQLIAKRVDGQDMDALRSMVDDLKGK
LGSAVIILGSANGEKVNLVAGVTKDLNDRGFHAGKLIKEVATRCGGGGGGRPDMAQAGGRDATKLDEALKFASHYVESLA

Sequences:

>Translated_880_residues
MKPLKSLTGAQIRQMYLDFFKSKGHSVEPSASLVPVEDPTLLWINSGVATLKKYFDGRVIPANPRIVNAQKSIRTNDIEN
VGKTARHHTFFEMLGNFSVGEYFREEAITWGWELLTSDEWYGLDPDRLSVTIHPDDEESKQIWMNLGMPADRIIPLEDNF
WEIGEGPSGPNTEIFFDRGPAFGDDPTDPELYPGGENERYLEIWNIVFSQYNHDGHGNYTELPRKNIDTGMGLERMASVL
QDVPTNFDTDLFMPIIRETEKISGDVYRQDSSKDVAFKVIADHIRTVSFAIGDGALPSNEGRGYVLRRLLRRAVRYAKML
GIERPFMYELVDVVGDIMVDFYPQVNEKKDFIKKVIKNEEERFHETLNDGLAILNEVAKAQKAAGSNIISGQDAFRLYDT
YGFPLELTIEYAEDHQMTVDEDGFKEAMNEQRERARQARADVESMQIQSGVLADITMPSTFLGYNNLETNAKIISLIHNG
ELADVVAAGEEAQVILDQTPFYAESGGQIGDKGQIVGEDFLLRVKDVSKAPNGQNLHTVTVESGVAKAEVAVTASVDQAS
RQAIVKNHTATHLLHRALKDTLGEHVNQAGSLVTPDRLRFDFSHFGQVTSEELQSIERQVNEKIWASIGVDIEEMNIADA
KAKGAMALFGEKYGDVVRVVSAGDYSVELCGGIHVSNTAEIGLFKILSESGIGAGTRRIEAVTGAGAYEVMNQQIETLEA
AAKLLKTKPLEVPARVQALQAEIKEVERASESLKAKLANIEASSLTDAIETIGGVQLIAKRVDGQDMDALRSMVDDLKGK
LGSAVIILGSANGEKVNLVAGVTKDLNDRGFHAGKLIKEVATRCGGGGGGRPDMAQAGGRDATKLDEALKFASHYVESLA
>Mature_880_residues
MKPLKSLTGAQIRQMYLDFFKSKGHSVEPSASLVPVEDPTLLWINSGVATLKKYFDGRVIPANPRIVNAQKSIRTNDIEN
VGKTARHHTFFEMLGNFSVGEYFREEAITWGWELLTSDEWYGLDPDRLSVTIHPDDEESKQIWMNLGMPADRIIPLEDNF
WEIGEGPSGPNTEIFFDRGPAFGDDPTDPELYPGGENERYLEIWNIVFSQYNHDGHGNYTELPRKNIDTGMGLERMASVL
QDVPTNFDTDLFMPIIRETEKISGDVYRQDSSKDVAFKVIADHIRTVSFAIGDGALPSNEGRGYVLRRLLRRAVRYAKML
GIERPFMYELVDVVGDIMVDFYPQVNEKKDFIKKVIKNEEERFHETLNDGLAILNEVAKAQKAAGSNIISGQDAFRLYDT
YGFPLELTIEYAEDHQMTVDEDGFKEAMNEQRERARQARADVESMQIQSGVLADITMPSTFLGYNNLETNAKIISLIHNG
ELADVVAAGEEAQVILDQTPFYAESGGQIGDKGQIVGEDFLLRVKDVSKAPNGQNLHTVTVESGVAKAEVAVTASVDQAS
RQAIVKNHTATHLLHRALKDTLGEHVNQAGSLVTPDRLRFDFSHFGQVTSEELQSIERQVNEKIWASIGVDIEEMNIADA
KAKGAMALFGEKYGDVVRVVSAGDYSVELCGGIHVSNTAEIGLFKILSESGIGAGTRRIEAVTGAGAYEVMNQQIETLEA
AAKLLKTKPLEVPARVQALQAEIKEVERASESLKAKLANIEASSLTDAIETIGGVQLIAKRVDGQDMDALRSMVDDLKGK
LGSAVIILGSANGEKVNLVAGVTKDLNDRGFHAGKLIKEVATRCGGGGGGRPDMAQAGGRDATKLDEALKFASHYVESLA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=975, Percent_Identity=35.3846153846154, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI38569417, Length=965, Percent_Identity=31.2953367875648, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI217416402, Length=396, Percent_Identity=23.989898989899, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789048, Length=876, Percent_Identity=46.689497716895, Blast_Score=756, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=981, Percent_Identity=34.7604485219164, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17536681, Length=750, Percent_Identity=34, Blast_Score=373, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324911, Length=856, Percent_Identity=39.2523364485981, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582809, Length=979, Percent_Identity=34.2185903983657, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45552267, Length=979, Percent_Identity=34.2185903983657, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24658214, Length=906, Percent_Identity=28.5871964679912, Blast_Score=313, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96775; Mature: 96775

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLKSLTGAQIRQMYLDFFKSKGHSVEPSASLVPVEDPTLLWINSGVATLKKYFDGRVI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHCCEEE
PANPRIVNAQKSIRTNDIENVGKTARHHTFFEMLGNFSVGEYFREEAITWGWELLTSDEW
CCCCCEECCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHEEECCCC
YGLDPDRLSVTIHPDDEESKQIWMNLGMPADRIIPLEDNFWEIGEGPSGPNTEIFFDRGP
CCCCCCCEEEEECCCCCCCHHHHEECCCCHHCEEECCCCCEECCCCCCCCCCEEEEECCC
AFGDDPTDPELYPGGENERYLEIWNIVFSQYNHDGHGNYTELPRKNIDTGMGLERMASVL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QDVPTNFDTDLFMPIIRETEKISGDVYRQDSSKDVAFKVIADHIRTVSFAIGDGALPSNE
HHCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHEEEECCCCCCCCC
GRGYVLRRLLRRAVRYAKMLGIERPFMYELVDVVGDIMVDFYPQVNEKKDFIKKVIKNEE
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
ERFHETLNDGLAILNEVAKAQKAAGSNIISGQDAFRLYDTYGFPLELTIEYAEDHQMTVD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
EDGFKEAMNEQRERARQARADVESMQIQSGVLADITMPSTFLGYNNLETNAKIISLIHNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCEEEEEEECC
ELADVVAAGEEAQVILDQTPFYAESGGQIGDKGQIVGEDFLLRVKDVSKAPNGQNLHTVT
CHHHHHHCCCCCEEEEECCCCEECCCCCCCCCCCEECHHHEEEEHHHCCCCCCCCEEEEE
VESGVAKAEVAVTASVDQASRQAIVKNHTATHLLHRALKDTLGEHVNQAGSLVTPDRLRF
ECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEE
DFSHFGQVTSEELQSIERQVNEKIWASIGVDIEEMNIADAKAKGAMALFGEKYGDVVRVV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHCCCHHHHHHHHHCCEEEEE
SAGDYSVELCGGIHVSNTAEIGLFKILSESGIGAGTRRIEAVTGAGAYEVMNQQIETLEA
ECCCCEEEEECCEEECCCCHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHHHHHHH
AAKLLKTKPLEVPARVQALQAEIKEVERASESLKAKLANIEASSLTDAIETIGGVQLIAK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RVDGQDMDALRSMVDDLKGKLGSAVIILGSANGEKVNLVAGVTKDLNDRGFHAGKLIKEV
HCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
ATRCGGGGGGRPDMAQAGGRDATKLDEALKFASHYVESLA
HHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPLKSLTGAQIRQMYLDFFKSKGHSVEPSASLVPVEDPTLLWINSGVATLKKYFDGRVI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHCCEEE
PANPRIVNAQKSIRTNDIENVGKTARHHTFFEMLGNFSVGEYFREEAITWGWELLTSDEW
CCCCCEECCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHEEECCCC
YGLDPDRLSVTIHPDDEESKQIWMNLGMPADRIIPLEDNFWEIGEGPSGPNTEIFFDRGP
CCCCCCCEEEEECCCCCCCHHHHEECCCCHHCEEECCCCCEECCCCCCCCCCEEEEECCC
AFGDDPTDPELYPGGENERYLEIWNIVFSQYNHDGHGNYTELPRKNIDTGMGLERMASVL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QDVPTNFDTDLFMPIIRETEKISGDVYRQDSSKDVAFKVIADHIRTVSFAIGDGALPSNE
HHCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHEEEECCCCCCCCC
GRGYVLRRLLRRAVRYAKMLGIERPFMYELVDVVGDIMVDFYPQVNEKKDFIKKVIKNEE
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
ERFHETLNDGLAILNEVAKAQKAAGSNIISGQDAFRLYDTYGFPLELTIEYAEDHQMTVD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
EDGFKEAMNEQRERARQARADVESMQIQSGVLADITMPSTFLGYNNLETNAKIISLIHNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCEEEEEEECC
ELADVVAAGEEAQVILDQTPFYAESGGQIGDKGQIVGEDFLLRVKDVSKAPNGQNLHTVT
CHHHHHHCCCCCEEEEECCCCEECCCCCCCCCCCEECHHHEEEEHHHCCCCCCCCEEEEE
VESGVAKAEVAVTASVDQASRQAIVKNHTATHLLHRALKDTLGEHVNQAGSLVTPDRLRF
ECCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEE
DFSHFGQVTSEELQSIERQVNEKIWASIGVDIEEMNIADAKAKGAMALFGEKYGDVVRVV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHCCCHHHHHHHHHCCEEEEE
SAGDYSVELCGGIHVSNTAEIGLFKILSESGIGAGTRRIEAVTGAGAYEVMNQQIETLEA
ECCCCEEEEECCEEECCCCHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHHHHHHH
AAKLLKTKPLEVPARVQALQAEIKEVERASESLKAKLANIEASSLTDAIETIGGVQLIAK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RVDGQDMDALRSMVDDLKGKLGSAVIILGSANGEKVNLVAGVTKDLNDRGFHAGKLIKEV
HCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
ATRCGGGGGGRPDMAQAGGRDATKLDEALKFASHYVESLA
HHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA