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The map label for this gene is hisS

Identifier: 229918418

GI number: 229918418

Start: 2674514

End: 2675797

Strand: Direct

Name: hisS

Synonym: EAT1b_2704

Alternate gene names: 229918418

Gene position: 2674514-2675797 (Clockwise)

Preceding gene: 229918417

Following gene: 229918419

Centisome position: 89.15

GC content: 53.66

Gene sequence:

>1284_bases
ATGATGAAAGCACCACGCGGTACACAAGACCTGCTTCCGGGCACGGTCGAGACGTGGCAATATGTCGAGGAAAAACTTCG
CGACATGAGCGCCCGTTACAACTATAAAGAAATCCGCACACCAATCTTTGAAACGACGAACCTGTTCACACGCAGTGTCG
GGGAAACGACGGACATCGTCCAAAAAGAGATGTTCACGCTCGTGAAGAAAGGGAAAGACGAGTACACCCTTCGCCCAGAA
GGGACGGCATCTGTCGTTCGTGCGTTCGTGACGAACAAGCTATTTGGAAATCCGGACCAACCGACGAAGCTCTACTATAT
GGGACCGATGTTCCGCTACGAGCGCCCGCAAAAAGGTCGCTTCCGTCAGCTTCACCAATACGGTGTCGAAGCGATCGGCA
GTAGTGACCCGGCCGTTGACGCTGAGGTCATCAGCCTCGCTTACTCGATTTACAAGTCGTTCGGACTTCAGAAAATAAAA
GTCGTCGTCAACTCGCTTGGGGACGCGGAAAGCCGCAACGCCCACCGCGATGCGCTCATCCGTCACTTCGAGCCGGTGGC
AGGTGAACTTTGCCAAGACTGCCAGTCACGTCTTTACAAGAACCCGCTTCGCATCTTGGATTGCAAGGTCGACCGCGATC
ACCCGTCGATGAAGACGGCACCGTCAATCCTTGAATATTTGAACGAAGAGTCGAAAGCTTACTTTGAGCAAGTGCTCGCA
CAACTTGACGCACTAGGCGTCGCGTATGAAGTCGACCCGACACTCGTTCGTGGACTCGATTACTATACGCACACGGCGTT
TGAAATCATGTCCGAGGCAGAAGGGTTCGGAGCCATCACGACACTTTGTGGCGGTGGACGCTATAACGGTCTCGTACAGG
ATATCGGCGGACCGGACATGCCGGGAATCGGTTTTGGGATCGGGTTGGAGCGCCTCATCCTCGCATTGGAGAATGAAGGA
GTCTTACCTGAACTCGAGACCGGACTTGATGTCTTCATCGTCGCCCAAGGCGAGAAAGAAGTCGAATTGACGGCGACACG
CCTCTTGCAACTCCTTCGCATGGAAGGGTTCAAGGCAGACCGTGATTACCAAGGACGCAAGTTCAAAGGACAATTCAAAC
AGGCGGACCGCCTGAAAGCCAAGTTCACAGTCATCTTAGGGGATGATGAAGTCGAGCGCGGGGCAGCCGCATTGAAAGAT
ATGACGACTGGTGAACAAGTCGACGTGCCACTCTCGGCCGTTGCAGACAAAATTCGTGAACTATCGGAAGGAGCAGGACA
ATGA

Upstream 100 bases:

>100_bases
TAATGGCTCTGCAACGAGGGTGGTACCACGGGAAACTTACACAGGCTCTCGTCCCTTTGCGGACGAGGGTCTTTTTGTAT
TTTTTAAGGAGGGAAATGTG

Downstream 100 bases:

>100_bases
TCGGACGCACACATATGAACGGCCGCGTGACAGAGTCACTCATCGGCCAAGAAGTACAATTAAAAGGATGGGTACAAAAA
CGTCGTGACCTCGGAGGACT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MMKAPRGTQDLLPGTVETWQYVEEKLRDMSARYNYKEIRTPIFETTNLFTRSVGETTDIVQKEMFTLVKKGKDEYTLRPE
GTASVVRAFVTNKLFGNPDQPTKLYYMGPMFRYERPQKGRFRQLHQYGVEAIGSSDPAVDAEVISLAYSIYKSFGLQKIK
VVVNSLGDAESRNAHRDALIRHFEPVAGELCQDCQSRLYKNPLRILDCKVDRDHPSMKTAPSILEYLNEESKAYFEQVLA
QLDALGVAYEVDPTLVRGLDYYTHTAFEIMSEAEGFGAITTLCGGGRYNGLVQDIGGPDMPGIGFGIGLERLILALENEG
VLPELETGLDVFIVAQGEKEVELTATRLLQLLRMEGFKADRDYQGRKFKGQFKQADRLKAKFTVILGDDEVERGAAALKD
MTTGEQVDVPLSAVADKIRELSEGAGQ

Sequences:

>Translated_427_residues
MMKAPRGTQDLLPGTVETWQYVEEKLRDMSARYNYKEIRTPIFETTNLFTRSVGETTDIVQKEMFTLVKKGKDEYTLRPE
GTASVVRAFVTNKLFGNPDQPTKLYYMGPMFRYERPQKGRFRQLHQYGVEAIGSSDPAVDAEVISLAYSIYKSFGLQKIK
VVVNSLGDAESRNAHRDALIRHFEPVAGELCQDCQSRLYKNPLRILDCKVDRDHPSMKTAPSILEYLNEESKAYFEQVLA
QLDALGVAYEVDPTLVRGLDYYTHTAFEIMSEAEGFGAITTLCGGGRYNGLVQDIGGPDMPGIGFGIGLERLILALENEG
VLPELETGLDVFIVAQGEKEVELTATRLLQLLRMEGFKADRDYQGRKFKGQFKQADRLKAKFTVILGDDEVERGAAALKD
MTTGEQVDVPLSAVADKIRELSEGAGQ
>Mature_427_residues
MMKAPRGTQDLLPGTVETWQYVEEKLRDMSARYNYKEIRTPIFETTNLFTRSVGETTDIVQKEMFTLVKKGKDEYTLRPE
GTASVVRAFVTNKLFGNPDQPTKLYYMGPMFRYERPQKGRFRQLHQYGVEAIGSSDPAVDAEVISLAYSIYKSFGLQKIK
VVVNSLGDAESRNAHRDALIRHFEPVAGELCQDCQSRLYKNPLRILDCKVDRDHPSMKTAPSILEYLNEESKAYFEQVLA
QLDALGVAYEVDPTLVRGLDYYTHTAFEIMSEAEGFGAITTLCGGGRYNGLVQDIGGPDMPGIGFGIGLERLILALENEG
VLPELETGLDVFIVAQGEKEVELTATRLLQLLRMEGFKADRDYQGRKFKGQFKQADRLKAKFTVILGDDEVERGAAALKD
MTTGEQVDVPLSAVADKIRELSEGAGQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=467, Percent_Identity=25.0535331905782, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788861, Length=425, Percent_Identity=42.5882352941177, Blast_Score=347, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI71993686, Length=476, Percent_Identity=22.0588235294118, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71993693, Length=465, Percent_Identity=20.8602150537634, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325290, Length=474, Percent_Identity=22.1518987341772, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24643061, Length=477, Percent_Identity=25.7861635220126, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24643059, Length=496, Percent_Identity=24.7983870967742, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_EXISA (C4L534)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887064.1
- GeneID:   7868908
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2704
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47760; Mature: 47760

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAPRGTQDLLPGTVETWQYVEEKLRDMSARYNYKEIRTPIFETTNLFTRSVGETTDIV
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCHHHHH
QKEMFTLVKKGKDEYTLRPEGTASVVRAFVTNKLFGNPDQPTKLYYMGPMFRYERPQKGR
HHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCHH
FRQLHQYGVEAIGSSDPAVDAEVISLAYSIYKSFGLQKIKVVVNSLGDAESRNAHRDALI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHH
RHFEPVAGELCQDCQSRLYKNPLRILDCKVDRDHPSMKTAPSILEYLNEESKAYFEQVLA
HHHCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
QLDALGVAYEVDPTLVRGLDYYTHTAFEIMSEAEGFGAITTLCGGGRYNGLVQDIGGPDM
HHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHCCCCCC
PGIGFGIGLERLILALENEGVLPELETGLDVFIVAQGEKEVELTATRLLQLLRMEGFKAD
CCCCHHHHHHHHHHHHCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
RDYQGRKFKGQFKQADRLKAKFTVILGDDEVERGAAALKDMTTGEQVDVPLSAVADKIRE
CCCCCCCCCCHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LSEGAGQ
HHHCCCC
>Mature Secondary Structure
MMKAPRGTQDLLPGTVETWQYVEEKLRDMSARYNYKEIRTPIFETTNLFTRSVGETTDIV
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCHHHHH
QKEMFTLVKKGKDEYTLRPEGTASVVRAFVTNKLFGNPDQPTKLYYMGPMFRYERPQKGR
HHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCHH
FRQLHQYGVEAIGSSDPAVDAEVISLAYSIYKSFGLQKIKVVVNSLGDAESRNAHRDALI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHH
RHFEPVAGELCQDCQSRLYKNPLRILDCKVDRDHPSMKTAPSILEYLNEESKAYFEQVLA
HHHCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
QLDALGVAYEVDPTLVRGLDYYTHTAFEIMSEAEGFGAITTLCGGGRYNGLVQDIGGPDM
HHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHCCCCCC
PGIGFGIGLERLILALENEGVLPELETGLDVFIVAQGEKEVELTATRLLQLLRMEGFKAD
CCCCHHHHHHHHHHHHCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
RDYQGRKFKGQFKQADRLKAKFTVILGDDEVERGAAALKDMTTGEQVDVPLSAVADKIRE
CCCCCCCCCCHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LSEGAGQ
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA