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The map label for this gene is aspS

Identifier: 229918419

GI number: 229918419

Start: 2675794

End: 2677566

Strand: Direct

Name: aspS

Synonym: EAT1b_2705

Alternate gene names: 229918419

Gene position: 2675794-2677566 (Clockwise)

Preceding gene: 229918418

Following gene: 229918421

Centisome position: 89.2

GC content: 53.53

Gene sequence:

>1773_bases
ATGATCGGACGCACACATATGAACGGCCGCGTGACAGAGTCACTCATCGGCCAAGAAGTACAATTAAAAGGATGGGTACA
AAAACGTCGTGACCTCGGAGGACTCATCTTCATCGACCTTCGTGATCGCACGGGAATCGTCCAAGTCGTCGTTCGCCCAG
AAAATGAAGCAGTCCACCAACTCGCGGAGACGATTCGTAGCGAGTATGTCCTCGACGTGAAAGGAACGGTTCTCGAGCGT
GAGAACAAGAACCCGAATATGCCGACAGGAAACATCGAAGTCATCGCGTCAGAGATCAACATCTTGAACGCAGCGAAGAT
GACACCGATCCCAATCGGTGATGAAGCAGAAAACGTCTCAGAAGACCTTCGCCTCAAATACCGTTACCTCGACCTTCGTC
GTCCAGCGCTTCAAGAGACGTTCCGTCTCCGCTCGAAGACGTCGAACACGATCCGTAACTTCTTGACGGAGCAAGAGTTC
CTCGAAGTCGAAACACCGATTTTGACGAAGTCGACACCAGAAGGCGCACGTGACTATCTCGTCCCGAGCCGTGTCCACGG
TGGTGAGTTCTACGCACTCCCGCAATCACCACAGCTCTTCAAACAGTTGCTCATGGTCGCAGGATTTGACCGTTACTTCC
AAATCGCACGCTGCTTCCGTGACGAGGACCTTCGTGCAGACCGTCAACCTGAATTCACGCAAGTCGACATCGAAACGAGC
TTCATGGACATCGAAGACTTGATGGCGATGATGGAATCGATGATGGGACAAGTCATGGAATCGACGCTCGGTCGTACGGA
CACACCAGCGAAGTTCGAGCGGATGACACACGCGGAAGCGATGCGTCGTTTTGGTTCGGATAAGCCGGACACGCGCTTCG
GTCTCGAACTCATCGACGTGGCAGACGCTGTGAAAGGTGCTGGCTTTAAAGTGTTCGATGCAGCACTCGAATCAAACGGG
CAAGTCAAGGCGATCAACGTCAAAGGTGAGGCGGACAACTTCTCACGTAAAGACATCGACAAGCTTCAAGAGTTCACGGC
GGTGTACGGTGCGAAAGGTCTCGCATGGCTCAAAGTGACAGCGGAAGGCTTGAACGGTCCAATCGCGAAATTCTTCGACG
AGACGTACGCGCAGAAGTTGATCGAAGCAACGAATGCAGAAGCAGGTGACCTTCTCCTCTTCGTCGCATCGAAACCAACT
GTCGTCGCAGACAGCCTTGGCGCACTCCGCGTCAAACTCGGCCACGAACTTGGTCTCATCGACGAGTCGAAGTTCAACTT
CCTATGGGTGACGGACTTCCCACTCGTGACGTTTGAAGAAGAAGACGGTCGCTTCTATGCGAACCACCATCCGTTCACGA
TGCCAAACCGTGAAGACCTTCACCTGCTCGAGACAGACCCGGCAAGCGTACGCGCGGTAGCGTATGACCTCGTCTTGAAC
GGTTACGAGCTCGGCGGCGGTTCACAGCGTATTTACGAGCGAGACATCCAAGAGCGTATGTTCAAATTGCTCGGCTTCAC
GCAAGAAGAAGCGGTTGAACAGTTCGGCTTCTTACTCGAAGCGTTCGAATATGGTACGCCACCACACGCGGGAATCGCAC
TCGGTCTCGACCGTCTCGTCATGATTCTTGCAGGTCGTTCGAACCTTCGCGACACAATCGCCTTCCCGAAAACGGCTTCG
GCAAGCTGTCTCTTGACGCAAGCACCGAGCCCTGTCGCGGACGCGCAACTCGAAGAGTTGTCGATTCGTACGGCAATAAA
AGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GGGCAGCCGCATTGAAAGATATGACGACTGGTGAACAAGTCGACGTGCCACTCTCGGCCGTTGCAGACAAAATTCGTGAA
CTATCGGAAGGAGCAGGACA

Downstream 100 bases:

>100_bases
GCATAAAAAAACGAGGGTTGCCATGGCAATCCTCGTTTTTTGTATGTGTGAAACGGATTTTGGATTCAACACGAGATGGT
AGATATTTAATCGGAACACA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MIGRTHMNGRVTESLIGQEVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVVRPENEAVHQLAETIRSEYVLDVKGTVLER
ENKNPNMPTGNIEVIASEINILNAAKMTPIPIGDEAENVSEDLRLKYRYLDLRRPALQETFRLRSKTSNTIRNFLTEQEF
LEVETPILTKSTPEGARDYLVPSRVHGGEFYALPQSPQLFKQLLMVAGFDRYFQIARCFRDEDLRADRQPEFTQVDIETS
FMDIEDLMAMMESMMGQVMESTLGRTDTPAKFERMTHAEAMRRFGSDKPDTRFGLELIDVADAVKGAGFKVFDAALESNG
QVKAINVKGEADNFSRKDIDKLQEFTAVYGAKGLAWLKVTAEGLNGPIAKFFDETYAQKLIEATNAEAGDLLLFVASKPT
VVADSLGALRVKLGHELGLIDESKFNFLWVTDFPLVTFEEEDGRFYANHHPFTMPNREDLHLLETDPASVRAVAYDLVLN
GYELGGGSQRIYERDIQERMFKLLGFTQEEAVEQFGFLLEAFEYGTPPHAGIALGLDRLVMILAGRSNLRDTIAFPKTAS
ASCLLTQAPSPVADAQLEELSIRTAIKEKK

Sequences:

>Translated_590_residues
MIGRTHMNGRVTESLIGQEVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVVRPENEAVHQLAETIRSEYVLDVKGTVLER
ENKNPNMPTGNIEVIASEINILNAAKMTPIPIGDEAENVSEDLRLKYRYLDLRRPALQETFRLRSKTSNTIRNFLTEQEF
LEVETPILTKSTPEGARDYLVPSRVHGGEFYALPQSPQLFKQLLMVAGFDRYFQIARCFRDEDLRADRQPEFTQVDIETS
FMDIEDLMAMMESMMGQVMESTLGRTDTPAKFERMTHAEAMRRFGSDKPDTRFGLELIDVADAVKGAGFKVFDAALESNG
QVKAINVKGEADNFSRKDIDKLQEFTAVYGAKGLAWLKVTAEGLNGPIAKFFDETYAQKLIEATNAEAGDLLLFVASKPT
VVADSLGALRVKLGHELGLIDESKFNFLWVTDFPLVTFEEEDGRFYANHHPFTMPNREDLHLLETDPASVRAVAYDLVLN
GYELGGGSQRIYERDIQERMFKLLGFTQEEAVEQFGFLLEAFEYGTPPHAGIALGLDRLVMILAGRSNLRDTIAFPKTAS
ASCLLTQAPSPVADAQLEELSIRTAIKEKK
>Mature_590_residues
MIGRTHMNGRVTESLIGQEVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVVRPENEAVHQLAETIRSEYVLDVKGTVLER
ENKNPNMPTGNIEVIASEINILNAAKMTPIPIGDEAENVSEDLRLKYRYLDLRRPALQETFRLRSKTSNTIRNFLTEQEF
LEVETPILTKSTPEGARDYLVPSRVHGGEFYALPQSPQLFKQLLMVAGFDRYFQIARCFRDEDLRADRQPEFTQVDIETS
FMDIEDLMAMMESMMGQVMESTLGRTDTPAKFERMTHAEAMRRFGSDKPDTRFGLELIDVADAVKGAGFKVFDAALESNG
QVKAINVKGEADNFSRKDIDKLQEFTAVYGAKGLAWLKVTAEGLNGPIAKFFDETYAQKLIEATNAEAGDLLLFVASKPT
VVADSLGALRVKLGHELGLIDESKFNFLWVTDFPLVTFEEEDGRFYANHHPFTMPNREDLHLLETDPASVRAVAYDLVLN
GYELGGGSQRIYERDIQERMFKLLGFTQEEAVEQFGFLLEAFEYGTPPHAGIALGLDRLVMILAGRSNLRDTIAFPKTAS
ASCLLTQAPSPVADAQLEELSIRTAIKEKK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=41.4023372287145, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI45439306, Length=265, Percent_Identity=28.6792452830189, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI5031815, Length=257, Percent_Identity=29.5719844357977, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI194272210, Length=257, Percent_Identity=29.5719844357977, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI226491502, Length=251, Percent_Identity=24.7011952191235, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4758762, Length=262, Percent_Identity=27.4809160305344, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=51.7706576728499, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1789256, Length=314, Percent_Identity=29.2993630573248, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790571, Length=304, Percent_Identity=28.9473684210526, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=36.3939899833055, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17551876, Length=246, Percent_Identity=27.6422764227642, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71994340, Length=255, Percent_Identity=24.3137254901961, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=255, Percent_Identity=24.3137254901961, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=255, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=637, Percent_Identity=34.0659340659341, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6323011, Length=243, Percent_Identity=28.8065843621399, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=257, Percent_Identity=26.4591439688716, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24584738, Length=595, Percent_Identity=38.9915966386555, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=254, Percent_Identity=29.9212598425197, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640849, Length=262, Percent_Identity=27.0992366412214, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640851, Length=262, Percent_Identity=27.0992366412214, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_EXISA (C4L535)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887065.1
- GeneID:   7868909
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2705
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66264; Mature: 66264

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRTHMNGRVTESLIGQEVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVVRPENEAVHQ
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCHHHHHH
LAETIRSEYVLDVKGTVLERENKNPNMPTGNIEVIASEINILNAAKMTPIPIGDEAENVS
HHHHHHHCEEEECCCCEEECCCCCCCCCCCCEEEEEEHHCCHHHCCCCCCCCCCCHHHHH
EDLRLKYRYLDLRRPALQETFRLRSKTSNTIRNFLTEQEFLEVETPILTKSTPEGARDYL
HHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHCC
VPSRVHGGEFYALPQSPQLFKQLLMVAGFDRYFQIARCFRDEDLRADRQPEFTQVDIETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHH
FMDIEDLMAMMESMMGQVMESTLGRTDTPAKFERMTHAEAMRRFGSDKPDTRFGLELIDV
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCHHHHHH
ADAVKGAGFKVFDAALESNGQVKAINVKGEADNFSRKDIDKLQEFTAVYGAKGLAWLKVT
HHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
AEGLNGPIAKFFDETYAQKLIEATNAEAGDLLLFVASKPTVVADSLGALRVKLGHELGLI
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCEEEEECCCCCCE
DESKFNFLWVTDFPLVTFEEEDGRFYANHHPFTMPNREDLHLLETDPASVRAVAYDLVLN
ECCCCCEEEEECCCEEEEECCCCEEEECCCCCCCCCCCCCEEEECCCCHHHHEEEEHHEE
GYELGGGSQRIYERDIQERMFKLLGFTQEEAVEQFGFLLEAFEYGTPPHAGIALGLDRLV
CEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MILAGRSNLRDTIAFPKTASASCLLTQAPSPVADAQLEELSIRTAIKEKK
HHHHCCCCCCHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIGRTHMNGRVTESLIGQEVQLKGWVQKRRDLGGLIFIDLRDRTGIVQVVVRPENEAVHQ
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCHHHHHH
LAETIRSEYVLDVKGTVLERENKNPNMPTGNIEVIASEINILNAAKMTPIPIGDEAENVS
HHHHHHHCEEEECCCCEEECCCCCCCCCCCCEEEEEEHHCCHHHCCCCCCCCCCCHHHHH
EDLRLKYRYLDLRRPALQETFRLRSKTSNTIRNFLTEQEFLEVETPILTKSTPEGARDYL
HHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHCC
VPSRVHGGEFYALPQSPQLFKQLLMVAGFDRYFQIARCFRDEDLRADRQPEFTQVDIETS
CCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHH
FMDIEDLMAMMESMMGQVMESTLGRTDTPAKFERMTHAEAMRRFGSDKPDTRFGLELIDV
HCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCHHHHHH
ADAVKGAGFKVFDAALESNGQVKAINVKGEADNFSRKDIDKLQEFTAVYGAKGLAWLKVT
HHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
AEGLNGPIAKFFDETYAQKLIEATNAEAGDLLLFVASKPTVVADSLGALRVKLGHELGLI
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEECCCCCCEEEEECCCCCCE
DESKFNFLWVTDFPLVTFEEEDGRFYANHHPFTMPNREDLHLLETDPASVRAVAYDLVLN
ECCCCCEEEEECCCEEEEECCCCEEEECCCCCCCCCCCCCEEEECCCCHHHHEEEEHHEE
GYELGGGSQRIYERDIQERMFKLLGFTQEEAVEQFGFLLEAFEYGTPPHAGIALGLDRLV
CEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MILAGRSNLRDTIAFPKTASASCLLTQAPSPVADAQLEELSIRTAIKEKK
HHHHCCCCCCHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA