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The map label for this gene is lplA [H]

Identifier: 229918187

GI number: 229918187

Start: 2422301

End: 2423290

Strand: Direct

Name: lplA [H]

Synonym: EAT1b_2467

Alternate gene names: 229918187

Gene position: 2422301-2423290 (Clockwise)

Preceding gene: 229918186

Following gene: 229918188

Centisome position: 80.75

GC content: 47.37

Gene sequence:

>990_bases
GTGAAATTTATTTTTAATCCGGATATCCGCGACCCAAAGATTAACTTGGCGGTCGAAGAGTATATCCTTAAACATCTGAA
CGTCGATCATGAAGATTACTTCCTGTTCTATATTAACGGTCCATCCATCATCATCGGGAAGAACCAAAACACATCAGAAG
AAGTCAACTTGAAGTACATCGAAGAGAATGGCATCGATGTCGTTCGACGTCTCTCTGGTGGCGGCGCCGTCTATCACGAT
GAAGGAAACTTGAACTTCAGCTTCATCACAAAAGACGATGGCAACTCGTTCAACAACTATAAGAAGTTCACACAACCGAT
CGTGGATGCGCTTCGTAACCTCGGTGTCGAGGCAGAGCTGACGGGTCGTAACGACATTCAGGTCGGTGAGCGTAAAATCA
GCGGGAACGCCCAATTCACGACGCGTGGACGCATGTTCAGCCACGGGACGCTCATGTTCAACTCGAACATCGAAGAGGTC
GTGAACTCGCTTAACGTCTCGGAAGAGAAAATGCGTTCAAAAGGAATCAAGTCCGTTCGTAGCCGTGTGGCGAACATCAC
TGAATTTTTAGAGCGTGAGATGGAGATGGAACATTTCGTCGAGGCGCTCCTCCATTCGATTTATGATGGAGAACAACCGG
AACGCTATGTGCTTCGTGACGAGGATTGGGAAATCGTCCATGATATCTCGAAAGAACGCTATGCGAACTGGGACTGGAAC
TTTGGTAAGTCGCCGAAGTTCGATGTTGAGTCGAAACGTCGTTTCCCAATCGGAACAATCGATGTCCGCTTGAACGTCAA
CAAAGGGACGATTACAGAAGCGAAAATTTACGGAGATTTCTTTGGTGTCGGTGACATCCAAGACGTCGAGCAAGCGCTCG
TTGGGACGCGTTTCGAACGTGCGGCCATCCGTGAGCAATTGATGCAACTCGATTTGAAACATTACTTCGGAAATGTCGAG
CTCGAGGAATTAGTCGATTTGATTAGTTAA

Upstream 100 bases:

>100_bases
AGCGCGTGATGACAGGTGAGTGGAAGTAAAAGATTTGTCGAGAACTCGTCTCGTTTCGTATAATACGAGTAACGAACATC
CGACTAGGAGGAAATAAACT

Downstream 100 bases:

>100_bases
GTTAGTCATGGCGGTCTCTACGGAGGTCGCCTTTTTTGGCAGTTTCGTGAACGTTGATCATACGGATTGCGCCTCGATGG
GAGAAGGCGTATCATAATTT

Product: lipoyltransferase and lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKFIFNPDIRDPKINLAVEEYILKHLNVDHEDYFLFYINGPSIIIGKNQNTSEEVNLKYIEENGIDVVRRLSGGGAVYHD
EGNLNFSFITKDDGNSFNNYKKFTQPIVDALRNLGVEAELTGRNDIQVGERKISGNAQFTTRGRMFSHGTLMFNSNIEEV
VNSLNVSEEKMRSKGIKSVRSRVANITEFLEREMEMEHFVEALLHSIYDGEQPERYVLRDEDWEIVHDISKERYANWDWN
FGKSPKFDVESKRRFPIGTIDVRLNVNKGTITEAKIYGDFFGVGDIQDVEQALVGTRFERAAIREQLMQLDLKHYFGNVE
LEELVDLIS

Sequences:

>Translated_329_residues
MKFIFNPDIRDPKINLAVEEYILKHLNVDHEDYFLFYINGPSIIIGKNQNTSEEVNLKYIEENGIDVVRRLSGGGAVYHD
EGNLNFSFITKDDGNSFNNYKKFTQPIVDALRNLGVEAELTGRNDIQVGERKISGNAQFTTRGRMFSHGTLMFNSNIEEV
VNSLNVSEEKMRSKGIKSVRSRVANITEFLEREMEMEHFVEALLHSIYDGEQPERYVLRDEDWEIVHDISKERYANWDWN
FGKSPKFDVESKRRFPIGTIDVRLNVNKGTITEAKIYGDFFGVGDIQDVEQALVGTRFERAAIREQLMQLDLKHYFGNVE
LEELVDLIS
>Mature_329_residues
MKFIFNPDIRDPKINLAVEEYILKHLNVDHEDYFLFYINGPSIIIGKNQNTSEEVNLKYIEENGIDVVRRLSGGGAVYHD
EGNLNFSFITKDDGNSFNNYKKFTQPIVDALRNLGVEAELTGRNDIQVGERKISGNAQFTTRGRMFSHGTLMFNSNIEEV
VNSLNVSEEKMRSKGIKSVRSRVANITEFLEREMEMEHFVEALLHSIYDGEQPERYVLRDEDWEIVHDISKERYANWDWN
FGKSPKFDVESKRRFPIGTIDVRLNVNKGTITEAKIYGDFFGVGDIQDVEQALVGTRFERAAIREQLMQLDLKHYFGNVE
LEELVDLIS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=243, Percent_Identity=31.2757201646091, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI21729884, Length=243, Percent_Identity=31.2757201646091, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI21729882, Length=243, Percent_Identity=31.2757201646091, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI21729880, Length=243, Percent_Identity=31.2757201646091, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI21729878, Length=243, Percent_Identity=31.2757201646091, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1790846, Length=325, Percent_Identity=34.1538461538462, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71981909, Length=242, Percent_Identity=33.8842975206612, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322415, Length=290, Percent_Identity=29.3103448275862, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI28573464, Length=269, Percent_Identity=29.368029739777, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38020; Mature: 38020

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIFNPDIRDPKINLAVEEYILKHLNVDHEDYFLFYINGPSIIIGKNQNTSEEVNLKYI
CCEEECCCCCCCEEEHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCEEEEEE
EENGIDVVRRLSGGGAVYHDEGNLNFSFITKDDGNSFNNYKKFTQPIVDALRNLGVEAEL
ECCCHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
TGRNDIQVGERKISGNAQFTTRGRMFSHGTLMFNSNIEEVVNSLNVSEEKMRSKGIKSVR
CCCCCCEECCEEECCCCEEEECCCEEECCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHH
SRVANITEFLEREMEMEHFVEALLHSIYDGEQPERYVLRDEDWEIVHDISKERYANWDWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCC
FGKSPKFDVESKRRFPIGTIDVRLNVNKGTITEAKIYGDFFGVGDIQDVEQALVGTRFER
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHH
AAIREQLMQLDLKHYFGNVELEELVDLIS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHC
>Mature Secondary Structure
MKFIFNPDIRDPKINLAVEEYILKHLNVDHEDYFLFYINGPSIIIGKNQNTSEEVNLKYI
CCEEECCCCCCCEEEHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCEEEEEE
EENGIDVVRRLSGGGAVYHDEGNLNFSFITKDDGNSFNNYKKFTQPIVDALRNLGVEAEL
ECCCHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
TGRNDIQVGERKISGNAQFTTRGRMFSHGTLMFNSNIEEVVNSLNVSEEKMRSKGIKSVR
CCCCCCEECCEEECCCCEEEECCCEEECCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHH
SRVANITEFLEREMEMEHFVEALLHSIYDGEQPERYVLRDEDWEIVHDISKERYANWDWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCC
FGKSPKFDVESKRRFPIGTIDVRLNVNKGTITEAKIYGDFFGVGDIQDVEQALVGTRFER
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHH
AAIREQLMQLDLKHYFGNVELEELVDLIS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]