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The map label for this gene is ywpJ [H]

Identifier: 229918186

GI number: 229918186

Start: 2421342

End: 2422229

Strand: Direct

Name: ywpJ [H]

Synonym: EAT1b_2466

Alternate gene names: 229918186

Gene position: 2421342-2422229 (Clockwise)

Preceding gene: 229918185

Following gene: 229918187

Centisome position: 80.71

GC content: 48.2

Gene sequence:

>888_bases
ATGGCAACGTATAAACTTTTTGTATCAGACATGGACGGTACGATTTTGCACAACCACACCAAAATCGCGGATGAGACAGC
GGAGATCGTTCGTGAAGCAGAGGCGGCAGGAATTCGCTTTGCGATTGCGACGGGGCGCAACTATGTGAATGCGAAACAGA
TTTTAGACGAGGTCGGACTCTCTTGCCCAATCGTATCGTCGAATGGGGGACGCGTGCTCGACACGTCGGGAGAAGTCCTT
CACGAAATCGATTTGACGATTGATGAGGCGCTTCAAGTCATCAACGTCCTTCATCAAGATGACAAGTATGCGGACGTCTT
TTACGAAATGTACACAGAAGCGGGACTCGTAGCGAGCCGTGGCGACTTGATTGAAGCATCGATGAAAAACTTGAAAGCGA
ATGGTGACGACCGCTCGCTCGAGATGTACGAATTCTTTAAGAATCGCTATTACGTCAATGAAGACGTCAAAATTGTTGAT
GACATCGAGGGCTTCATTCGTGAAGAAGCAGGACGTGTCTATAAGTTCATCGCGTTCTCAAGTCATCTCGAGAAGATGAA
CGGGCTTTGGAAAGAAATCGAAGACCAAGTCGAAGGAGTGTATGTCACGTCTTCAGGCAATGACAACATCGAAGTGATGG
CACACGGTGCAGATAAAGGGAACGGTGTGACAAAACTTGCTGAACACTATGGCATCGATTTATCTGAAGTCGTCGTCATC
GGGGATAACTTGAATGACGTGCCGATGTTCAAAGTGGCCGGAAAAGGAATTGCGATGGGCAATAGCCACGAAGACTTGAT
TGCCATCAGTGACCATGTCACCGAGCGTCATGATGAGTATGGTGTGGCGAACGCATTGAAGCGCGTGATGACAGGTGAGT
GGAAGTAA

Upstream 100 bases:

>100_bases
TGAAAAAAATTGAGATTTGATGAAGGGAGGGTTCGGATTGTTCCGCAACCCTCTCTTTTTGTTATAGTTAGAAGCGAAAG
AGAAAAAGGAGTGATGGAAG

Downstream 100 bases:

>100_bases
AAGATTTGTCGAGAACTCGTCTCGTTTCGTATAATACGAGTAACGAACATCCGACTAGGAGGAAATAAACTGTGAAATTT
ATTTTTAATCCGGATATCCG

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MATYKLFVSDMDGTILHNHTKIADETAEIVREAEAAGIRFAIATGRNYVNAKQILDEVGLSCPIVSSNGGRVLDTSGEVL
HEIDLTIDEALQVINVLHQDDKYADVFYEMYTEAGLVASRGDLIEASMKNLKANGDDRSLEMYEFFKNRYYVNEDVKIVD
DIEGFIREEAGRVYKFIAFSSHLEKMNGLWKEIEDQVEGVYVTSSGNDNIEVMAHGADKGNGVTKLAEHYGIDLSEVVVI
GDNLNDVPMFKVAGKGIAMGNSHEDLIAISDHVTERHDEYGVANALKRVMTGEWK

Sequences:

>Translated_295_residues
MATYKLFVSDMDGTILHNHTKIADETAEIVREAEAAGIRFAIATGRNYVNAKQILDEVGLSCPIVSSNGGRVLDTSGEVL
HEIDLTIDEALQVINVLHQDDKYADVFYEMYTEAGLVASRGDLIEASMKNLKANGDDRSLEMYEFFKNRYYVNEDVKIVD
DIEGFIREEAGRVYKFIAFSSHLEKMNGLWKEIEDQVEGVYVTSSGNDNIEVMAHGADKGNGVTKLAEHYGIDLSEVVVI
GDNLNDVPMFKVAGKGIAMGNSHEDLIAISDHVTERHDEYGVANALKRVMTGEWK
>Mature_294_residues
ATYKLFVSDMDGTILHNHTKIADETAEIVREAEAAGIRFAIATGRNYVNAKQILDEVGLSCPIVSSNGGRVLDTSGEVLH
EIDLTIDEALQVINVLHQDDKYADVFYEMYTEAGLVASRGDLIEASMKNLKANGDDRSLEMYEFFKNRYYVNEDVKIVDD
IEGFIREEAGRVYKFIAFSSHLEKMNGLWKEIEDQVEGVYVTSSGNDNIEVMAHGADKGNGVTKLAEHYGIDLSEVVVIG
DNLNDVPMFKVAGKGIAMGNSHEDLIAISDHVTERHDEYGVANALKRVMTGEWK

Specific function: Putative phosphatase (Potential) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI48994981, Length=270, Percent_Identity=29.2592592592593, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786982, Length=263, Percent_Identity=25.8555133079848, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081741, Length=271, Percent_Identity=25.830258302583, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081790, Length=297, Percent_Identity=26.2626262626263, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 32747; Mature: 32616

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYKLFVSDMDGTILHNHTKIADETAEIVREAEAAGIRFAIATGRNYVNAKQILDEVGL
CCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCC
SCPIVSSNGGRVLDTSGEVLHEIDLTIDEALQVINVLHQDDKYADVFYEMYTEAGLVASR
CCEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECC
GDLIEASMKNLKANGDDRSLEMYEFFKNRYYVNEDVKIVDDIEGFIREEAGRVYKFIAFS
CCHHHHHHHHCCCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHEEHHHHHH
SHLEKMNGLWKEIEDQVEGVYVTSSGNDNIEVMAHGADKGNGVTKLAEHYGIDLSEVVVI
HHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHEEEE
GDNLNDVPMFKVAGKGIAMGNSHEDLIAISDHVTERHDEYGVANALKRVMTGEWK
CCCCCCCCEEEECCCCEECCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATYKLFVSDMDGTILHNHTKIADETAEIVREAEAAGIRFAIATGRNYVNAKQILDEVGL
CEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHCC
SCPIVSSNGGRVLDTSGEVLHEIDLTIDEALQVINVLHQDDKYADVFYEMYTEAGLVASR
CCEEEECCCCEEEECCCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEECC
GDLIEASMKNLKANGDDRSLEMYEFFKNRYYVNEDVKIVDDIEGFIREEAGRVYKFIAFS
CCHHHHHHHHCCCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHEEHHHHHH
SHLEKMNGLWKEIEDQVEGVYVTSSGNDNIEVMAHGADKGNGVTKLAEHYGIDLSEVVVI
HHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHEEEE
GDNLNDVPMFKVAGKGIAMGNSHEDLIAISDHVTERHDEYGVANALKRVMTGEWK
CCCCCCCCEEEECCCCEECCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]