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The map label for this gene is purC

Identifier: 229917947

GI number: 229917947

Start: 2173768

End: 2174472

Strand: Direct

Name: purC

Synonym: EAT1b_2226

Alternate gene names: 229917947

Gene position: 2173768-2174472 (Clockwise)

Preceding gene: 229917946

Following gene: 229917948

Centisome position: 72.46

GC content: 47.52

Gene sequence:

>705_bases
ATGAAAGCAATTTATGAGGGCAAGGCGAAGGCGTTATTTGAGACAGAAGAAGCGGGTGTGCTTCGTGTGTACTATAAAGA
CGATGCGACAGCATTCAATGGAGAAAAGAAAGAGTCGATTGATGGGAAGGGGATTTTAAACAATGCCATCACGACCCAGT
TGTTCGAATTGATGCATGACAACAAGATTCCGACGCACTTTATCAAACAAATCTCAGACCGGGAACAGTTGGTCCGCCGT
GTCGACATCATTCCGTTAGAAGTGGTCGTCCGCAACGTCGCGGCCGGTAGTCTGGCGAAACGTCTCGGTTGGGAAGAAGG
AACACCGCTCCTCGCACCGATTGTCGAGTTTTATTATAAGGACGATGCGCTTGGGGACCCACTCCTCACGGAGGATCATA
TCCGATTATTGCAAGTTGCTACACACAAAGAAGTCGAAACATTGCGACAACTCGGGCTTTGGGTAAACGATGTCTTGCTC
GATTTCTTTGGTCAACACGGCATCGACGTGATCGATTTCAAACTAGAGTTCGGAAAAGTGGATGGGACGATCATCTTGGC
GGATGAAATCTCGCCGGATACGTGCCGCCTTTGGGACAAAGAAACGAAACAAAAGCTTGATAAAGATGTATTCCGTCGCG
ATTTAGGTTCTTTGACTGAGACGTATGGACAATTGTTAACACGCATAGGGGGAAACGGACAATGA

Upstream 100 bases:

>100_bases
CGTTATCACGTACGCCGTGTCGATACGATTTTTGAACGCTGTGGTTTGTAATCGTTGAGGGGCGACAAGCCCCTCTCACT
TTTAAGGGGGAACGGTTCAG

Downstream 100 bases:

>100_bases
AAAAAGTAAATGTGTATGTGACATTACGTGAAAGTGTACTCGACCCACAAGGAGTGGCCGTAAAAGGCGGACTCGAACAT
CTTGGATTCGCTGGAGTTGA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKAIYEGKAKALFETEEAGVLRVYYKDDATAFNGEKKESIDGKGILNNAITTQLFELMHDNKIPTHFIKQISDREQLVRR
VDIIPLEVVVRNVAAGSLAKRLGWEEGTPLLAPIVEFYYKDDALGDPLLTEDHIRLLQVATHKEVETLRQLGLWVNDVLL
DFFGQHGIDVIDFKLEFGKVDGTIILADEISPDTCRLWDKETKQKLDKDVFRRDLGSLTETYGQLLTRIGGNGQ

Sequences:

>Translated_234_residues
MKAIYEGKAKALFETEEAGVLRVYYKDDATAFNGEKKESIDGKGILNNAITTQLFELMHDNKIPTHFIKQISDREQLVRR
VDIIPLEVVVRNVAAGSLAKRLGWEEGTPLLAPIVEFYYKDDALGDPLLTEDHIRLLQVATHKEVETLRQLGLWVNDVLL
DFFGQHGIDVIDFKLEFGKVDGTIILADEISPDTCRLWDKETKQKLDKDVFRRDLGSLTETYGQLLTRIGGNGQ
>Mature_234_residues
MKAIYEGKAKALFETEEAGVLRVYYKDDATAFNGEKKESIDGKGILNNAITTQLFELMHDNKIPTHFIKQISDREQLVRR
VDIIPLEVVVRNVAAGSLAKRLGWEEGTPLLAPIVEFYYKDDALGDPLLTEDHIRLLQVATHKEVETLRQLGLWVNDVLL
DFFGQHGIDVIDFKLEFGKVDGTIILADEISPDTCRLWDKETKQKLDKDVFRRDLGSLTETYGQLLTRIGGNGQ

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=239, Percent_Identity=31.3807531380753, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI5453539, Length=239, Percent_Identity=31.3807531380753, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI119220559, Length=246, Percent_Identity=30.8943089430894, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI1788820, Length=227, Percent_Identity=40.9691629955947, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17531275, Length=234, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI18860083, Length=230, Percent_Identity=34.7826086956522, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24583917, Length=248, Percent_Identity=29.0322580645161, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_EXISA (C4L296)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886593.1
- ProteinModelPortal:   C4L296
- GeneID:   7870813
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2226
- OMA:   YKDDALG
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 26488; Mature: 26488

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIYEGKAKALFETEEAGVLRVYYKDDATAFNGEKKESIDGKGILNNAITTQLFELMHD
CCCCCCCCHHHHEECCCCCEEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHC
NKIPTHFIKQISDREQLVRRVDIIPLEVVVRNVAAGSLAKRLGWEEGTPLLAPIVEFYYK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
DDALGDPLLTEDHIRLLQVATHKEVETLRQLGLWVNDVLLDFFGQHGIDVIDFKLEFGKV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
DGTIILADEISPDTCRLWDKETKQKLDKDVFRRDLGSLTETYGQLLTRIGGNGQ
CCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKAIYEGKAKALFETEEAGVLRVYYKDDATAFNGEKKESIDGKGILNNAITTQLFELMHD
CCCCCCCCHHHHEECCCCCEEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHC
NKIPTHFIKQISDREQLVRRVDIIPLEVVVRNVAAGSLAKRLGWEEGTPLLAPIVEFYYK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
DDALGDPLLTEDHIRLLQVATHKEVETLRQLGLWVNDVLLDFFGQHGIDVIDFKLEFGKV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
DGTIILADEISPDTCRLWDKETKQKLDKDVFRRDLGSLTETYGQLLTRIGGNGQ
CCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA