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The map label for this gene is purB [H]

Identifier: 229917946

GI number: 229917946

Start: 2172426

End: 2173718

Strand: Direct

Name: purB [H]

Synonym: EAT1b_2225

Alternate gene names: 229917946

Gene position: 2172426-2173718 (Clockwise)

Preceding gene: 229917945

Following gene: 229917947

Centisome position: 72.42

GC content: 48.65

Gene sequence:

>1293_bases
ATGATTTCACGCTACACCCGCCCAGAGATGGCAAACATTTGGACTGATCAAAACAAATTCGAGGCTTGGTTAAAAGTTGA
ACTTTATGCCTGTGAGGCTTGGAGTGAACTCGGTATCATCCCGAAAGAAGATGTACAAGTTCTTTGGGACAAGGCATCGT
TCGATGTTGATCGCATTCTAGAGATTGAACAAGAGACACGTCATGATGTGATTGCGTTCACTCGTGCCGTTTCCGAGACA
CTCGGAGAAGAGAAAAAGTGGGTACATTACGGATTGACGTCGACGGATGTCGTCGACACGGCACTCTCGTACTTAATCAA
ACAGGCGAATGATCTTTTAGAAGCCGATTTAGATCGCTTCATCGGTATTCTCGCAGAAAAAGCGAAGGCACATAAGTATG
ACATCATGATGGGACGTACCCATGGCGTTCACGCGGAACCGACCACGTTCGGTCTGAAGCTCGCCCTCTGGTATGAAGAG
ATGAAACGAAACAAAAAACGCTTCATGGCAGCTCGCGAAACGATTGAATTCGGGAAGATGAGCGGAGCGGTTGGGACGTT
TGCGAATATCGACCCGTTCATCGAGTCATACGTCTGTGACAAGCTCGGTATCCAAGCTGCACCGATTTCGACACAGACAC
TCCAACGTGACCGTCATGCCGAGTACATGTCAACACTCGCATTGATTGCCACATCGATTGAAAAGATGGCAACCGAGATT
CGTGGACTTCAAAAAACAGAGACACGCGAAGTGGAAGAGTTCTTTGCGAAAGGACAAAAAGGGTCTTCTGCGATGCCGCA
TAAACGTAATCCAATCGGTTCTGAAAATATGACGGGTCTTGCACGCGTCATTCGCGGTCATATGATGACAGCATACGAGA
ATGTCCCACTCTGGCATGAACGTGACATCTCGCACTCATCAGCAGAACGAATCATCATTCCGGATGCGACGATTCTCCTC
AACTACATGTTGAACCGTTTCGGTAACATCGTGAAGAACTTGACGGTCTTCCCGGACAACATGCGCCGCAATATGGACCG
TACGTTCGGGATCATCTTCTCGCAACGGATCTTACTCGGTCTCATCGAGAAGGGCTGGTCACGTGAAGCAGCATACGACG
CGGTTCAACCGAAAGCGATGCAGGCATGGGAAGAAGAGACGATGTTCCGTACATTGATTGAAGAGGACAAAGAGATGGGC
GCACTCTTTACGAGTGAAGAGCTCGATGAGTTGTTCGACCCGCGTTATCACGTACGCCGTGTCGATACGATTTTTGAACG
CTGTGGTTTGTAA

Upstream 100 bases:

>100_bases
AGACAGGTCGCAAAATGGGACATTTGAACGTCCTTGGTGACACGGTCGAAGAAGCGCTTCATACCATCGAACAACTGGCA
ATTTGGAAGGAGACCGTTTT

Downstream 100 bases:

>100_bases
TCGTTGAGGGGCGACAAGCCCCTCTCACTTTTAAGGGGGAACGGTTCAGATGAAAGCAATTTATGAGGGCAAGGCGAAGG
CGTTATTTGAGACAGAAGAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MISRYTRPEMANIWTDQNKFEAWLKVELYACEAWSELGIIPKEDVQVLWDKASFDVDRILEIEQETRHDVIAFTRAVSET
LGEEKKWVHYGLTSTDVVDTALSYLIKQANDLLEADLDRFIGILAEKAKAHKYDIMMGRTHGVHAEPTTFGLKLALWYEE
MKRNKKRFMAARETIEFGKMSGAVGTFANIDPFIESYVCDKLGIQAAPISTQTLQRDRHAEYMSTLALIATSIEKMATEI
RGLQKTETREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMMTAYENVPLWHERDISHSSAERIIIPDATILL
NYMLNRFGNIVKNLTVFPDNMRRNMDRTFGIIFSQRILLGLIEKGWSREAAYDAVQPKAMQAWEEETMFRTLIEEDKEMG
ALFTSEELDELFDPRYHVRRVDTIFERCGL

Sequences:

>Translated_430_residues
MISRYTRPEMANIWTDQNKFEAWLKVELYACEAWSELGIIPKEDVQVLWDKASFDVDRILEIEQETRHDVIAFTRAVSET
LGEEKKWVHYGLTSTDVVDTALSYLIKQANDLLEADLDRFIGILAEKAKAHKYDIMMGRTHGVHAEPTTFGLKLALWYEE
MKRNKKRFMAARETIEFGKMSGAVGTFANIDPFIESYVCDKLGIQAAPISTQTLQRDRHAEYMSTLALIATSIEKMATEI
RGLQKTETREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMMTAYENVPLWHERDISHSSAERIIIPDATILL
NYMLNRFGNIVKNLTVFPDNMRRNMDRTFGIIFSQRILLGLIEKGWSREAAYDAVQPKAMQAWEEETMFRTLIEEDKEMG
ALFTSEELDELFDPRYHVRRVDTIFERCGL
>Mature_430_residues
MISRYTRPEMANIWTDQNKFEAWLKVELYACEAWSELGIIPKEDVQVLWDKASFDVDRILEIEQETRHDVIAFTRAVSET
LGEEKKWVHYGLTSTDVVDTALSYLIKQANDLLEADLDRFIGILAEKAKAHKYDIMMGRTHGVHAEPTTFGLKLALWYEE
MKRNKKRFMAARETIEFGKMSGAVGTFANIDPFIESYVCDKLGIQAAPISTQTLQRDRHAEYMSTLALIATSIEKMATEI
RGLQKTETREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMMTAYENVPLWHERDISHSSAERIIIPDATILL
NYMLNRFGNIVKNLTVFPDNMRRNMDRTFGIIFSQRILLGLIEKGWSREAAYDAVQPKAMQAWEEETMFRTLIEEDKEMG
ALFTSEELDELFDPRYHVRRVDTIFERCGL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=452, Percent_Identity=30.7522123893805, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=31.25, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI1787376, Length=317, Percent_Identity=27.1293375394322, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=406, Percent_Identity=26.6009852216749, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=363, Percent_Identity=26.9972451790634, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323391, Length=447, Percent_Identity=29.7539149888143, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24647570, Length=402, Percent_Identity=26.3681592039801, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49557; Mature: 49557

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRYTRPEMANIWTDQNKFEAWLKVELYACEAWSELGIIPKEDVQVLWDKASFDVDRIL
CCCCCCCCCHHHHCCCCHHHEEEEEEEEEEEHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
EIEQETRHDVIAFTRAVSETLGEEKKWVHYGLTSTDVVDTALSYLIKQANDLLEADLDRF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGILAEKAKAHKYDIMMGRTHGVHAEPTTFGLKLALWYEEMKRNKKRFMAARETIEFGKM
HHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPFIESYVCDKLGIQAAPISTQTLQRDRHAEYMSTLALIATSIEKMATEI
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKTETREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMMTAYENVPLWHE
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
RDISHSSAERIIIPDATILLNYMLNRFGNIVKNLTVFPDNMRRNMDRTFGIIFSQRILLG
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIEKGWSREAAYDAVQPKAMQAWEEETMFRTLIEEDKEMGALFTSEELDELFDPRYHVRR
HHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VDTIFERCGL
HHHHHHHHCC
>Mature Secondary Structure
MISRYTRPEMANIWTDQNKFEAWLKVELYACEAWSELGIIPKEDVQVLWDKASFDVDRIL
CCCCCCCCCHHHHCCCCHHHEEEEEEEEEEEHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
EIEQETRHDVIAFTRAVSETLGEEKKWVHYGLTSTDVVDTALSYLIKQANDLLEADLDRF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGILAEKAKAHKYDIMMGRTHGVHAEPTTFGLKLALWYEEMKRNKKRFMAARETIEFGKM
HHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPFIESYVCDKLGIQAAPISTQTLQRDRHAEYMSTLALIATSIEKMATEI
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKTETREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMMTAYENVPLWHE
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
RDISHSSAERIIIPDATILLNYMLNRFGNIVKNLTVFPDNMRRNMDRTFGIIFSQRILLG
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIEKGWSREAAYDAVQPKAMQAWEEETMFRTLIEEDKEMGALFTSEELDELFDPRYHVRR
HHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VDTIFERCGL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]