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The map label for this gene is purK [H]

Identifier: 229917945

GI number: 229917945

Start: 2171335

End: 2172429

Strand: Direct

Name: purK [H]

Synonym: EAT1b_2224

Alternate gene names: 229917945

Gene position: 2171335-2172429 (Clockwise)

Preceding gene: 229917944

Following gene: 229917946

Centisome position: 72.38

GC content: 48.4

Gene sequence:

>1095_bases
ATGAGAATCGGAATTTTAGGTGGAGGACAACTTGGACGGATGATGTCACTCAGTGCGAGTGCAATGGGATACACGACACT
TTGTCTTGACCCGAACCCCAATGCACCGACTGCTCAAGTGGCAGATTCAATTGTTGCCTCATTTGATGATGTCGATGCCG
CAAAAGAACTCGCTGCTCGTTCAGACGTTGTGACGTATGAATTTGAGAACATCGATACGGAAATGATTGCAGCCATCGAA
GAAAAATGCCCACAAGGTATGGACGTGTTGAAGGCGACGCAACATCGCATCTTAGAGAAACAGATGATTGAATCAGCGGG
ACTACGTGTCGCACCATATGTTTCGGTACGTACGGTTCGAGATGTACGGCAGGCATATGAGAAGCTCGGCTACCCATTTG
TCATCAAAACGTGTCGTTTCGGCTATGATGGGAAAGGGCAAACGGTCATTCGGTCGGAACATGACCTTCAGACCTTCGCT
GAACATTTTGTCGAACAGGAATACGTCGCCGAAGCTTGGTTGCCATTTGAAAAAGAAATTTCAGTCATTGTGACGAGAGG
GTATGACGAAACGTCGACATTCCCAATCAGTGAAAACATTCATCACAACAACATCCTACATTTATCGATTGTACCGGCTC
GCGTATCAAATGAGATTCAAGAGGATGCAAGACACCTCGCTCTCCAGCTTGCAGACCATATCGGCTTAATGGGAACGCTC
GCTGTCGAGCTGTTCGTCATGGCGGATGGCCGTCTATATGTGAATGAGCTTGCGCCGCGTCCGCACAACTCCGGGCACTA
CACGATTGACGCTTGTGAAACCTCACAATTTGAACAACACATTCGAGCCGTGACCGGGATGCGCCTCGGACGGACGAGAT
TGACATCGCCTGTCGTCATGGTCAACCTTCTCGGCGAGCATATGCCACGACTTCATGTCAAAGCATTGCCATCGAATGTT
AAACTACATCTGTATGGAAAAGATGAAGCGAAGACAGGTCGCAAAATGGGACATTTGAACGTCCTTGGTGACACGGTCGA
AGAAGCGCTTCATACCATCGAACAACTGGCAATTTGGAAGGAGACCGTTTTATGA

Upstream 100 bases:

>100_bases
AGATCCTCAGCATCCACTCCACAGACATAACACATCGACTGGATGCATTTCGTGAGAAGAAACAAGAGATGGCGATGCGA
GGAACGGAGGAGTTGGTCTG

Downstream 100 bases:

>100_bases
TTTCACGCTACACCCGCCCAGAGATGGCAAACATTTGGACTGATCAAAACAAATTCGAGGCTTGGTTAAAAGTTGAACTT
TATGCCTGTGAGGCTTGGAG

Product: phosphoribosylaminoimidazole carboxylase, ATPase

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MRIGILGGGQLGRMMSLSASAMGYTTLCLDPNPNAPTAQVADSIVASFDDVDAAKELAARSDVVTYEFENIDTEMIAAIE
EKCPQGMDVLKATQHRILEKQMIESAGLRVAPYVSVRTVRDVRQAYEKLGYPFVIKTCRFGYDGKGQTVIRSEHDLQTFA
EHFVEQEYVAEAWLPFEKEISVIVTRGYDETSTFPISENIHHNNILHLSIVPARVSNEIQEDARHLALQLADHIGLMGTL
AVELFVMADGRLYVNELAPRPHNSGHYTIDACETSQFEQHIRAVTGMRLGRTRLTSPVVMVNLLGEHMPRLHVKALPSNV
KLHLYGKDEAKTGRKMGHLNVLGDTVEEALHTIEQLAIWKETVL

Sequences:

>Translated_364_residues
MRIGILGGGQLGRMMSLSASAMGYTTLCLDPNPNAPTAQVADSIVASFDDVDAAKELAARSDVVTYEFENIDTEMIAAIE
EKCPQGMDVLKATQHRILEKQMIESAGLRVAPYVSVRTVRDVRQAYEKLGYPFVIKTCRFGYDGKGQTVIRSEHDLQTFA
EHFVEQEYVAEAWLPFEKEISVIVTRGYDETSTFPISENIHHNNILHLSIVPARVSNEIQEDARHLALQLADHIGLMGTL
AVELFVMADGRLYVNELAPRPHNSGHYTIDACETSQFEQHIRAVTGMRLGRTRLTSPVVMVNLLGEHMPRLHVKALPSNV
KLHLYGKDEAKTGRKMGHLNVLGDTVEEALHTIEQLAIWKETVL
>Mature_364_residues
MRIGILGGGQLGRMMSLSASAMGYTTLCLDPNPNAPTAQVADSIVASFDDVDAAKELAARSDVVTYEFENIDTEMIAAIE
EKCPQGMDVLKATQHRILEKQMIESAGLRVAPYVSVRTVRDVRQAYEKLGYPFVIKTCRFGYDGKGQTVIRSEHDLQTFA
EHFVEQEYVAEAWLPFEKEISVIVTRGYDETSTFPISENIHHNNILHLSIVPARVSNEIQEDARHLALQLADHIGLMGTL
AVELFVMADGRLYVNELAPRPHNSGHYTIDACETSQFEQHIRAVTGMRLGRTRLTSPVVMVNLLGEHMPRLHVKALPSNV
KLHLYGKDEAKTGRKMGHLNVLGDTVEEALHTIEQLAIWKETVL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=360, Percent_Identity=30.8333333333333, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788155, Length=380, Percent_Identity=25.2631578947368, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324702, Length=374, Percent_Identity=33.1550802139037, Blast_Score=213, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40572; Mature: 40572

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGILGGGQLGRMMSLSASAMGYTTLCLDPNPNAPTAQVADSIVASFDDVDAAKELAAR
CEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHC
SDVVTYEFENIDTEMIAAIEEKCPQGMDVLKATQHRILEKQMIESAGLRVAPYVSVRTVR
CCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHH
DVRQAYEKLGYPFVIKTCRFGYDGKGQTVIRSEHDLQTFAEHFVEQEYVAEAWLPFEKEI
HHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCE
SVIVTRGYDETSTFPISENIHHNNILHLSIVPARVSNEIQEDARHLALQLADHIGLMGTL
EEEEEECCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVELFVMADGRLYVNELAPRPHNSGHYTIDACETSQFEQHIRAVTGMRLGRTRLTSPVVM
HHEEEEEECCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHCCHHHH
VNLLGEHMPRLHVKALPSNVKLHLYGKDEAKTGRKMGHLNVLGDTVEEALHTIEQLAIWK
HHHHHHHCCCEEEEECCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ETVL
HHCC
>Mature Secondary Structure
MRIGILGGGQLGRMMSLSASAMGYTTLCLDPNPNAPTAQVADSIVASFDDVDAAKELAAR
CEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHC
SDVVTYEFENIDTEMIAAIEEKCPQGMDVLKATQHRILEKQMIESAGLRVAPYVSVRTVR
CCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHH
DVRQAYEKLGYPFVIKTCRFGYDGKGQTVIRSEHDLQTFAEHFVEQEYVAEAWLPFEKEI
HHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCE
SVIVTRGYDETSTFPISENIHHNNILHLSIVPARVSNEIQEDARHLALQLADHIGLMGTL
EEEEEECCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVELFVMADGRLYVNELAPRPHNSGHYTIDACETSQFEQHIRAVTGMRLGRTRLTSPVVM
HHEEEEEECCEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHCCHHHH
VNLLGEHMPRLHVKALPSNVKLHLYGKDEAKTGRKMGHLNVLGDTVEEALHTIEQLAIWK
HHHHHHHCCCEEEEECCCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ETVL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]