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The map label for this gene is mnmG [H]

Identifier: 229917462

GI number: 229917462

Start: 1705837

End: 1707723

Strand: Direct

Name: mnmG [H]

Synonym: EAT1b_1737

Alternate gene names: 229917462

Gene position: 1705837-1707723 (Clockwise)

Preceding gene: 229917461

Following gene: 229917463

Centisome position: 56.86

GC content: 50.4

Gene sequence:

>1887_bases
ATGGCTTATACGGCAGGCGAATTTGACGTCATCGTCATCGGGGCCGGACACGCAGGGATTGAAGCAGCACTTGCATCGGC
TCGCATGGGCGCGAAAACAGCGATGCTCACCATGAACCCCGATATGGTCGGGTTCATGTATTGCAACCCATCAATCGGTG
GGCCGGCGAAAGGAATCGTCGTACGTGAAATCGATGCGCTCGGTGGCGAGATGGGGCGCGCGATTGACGCGACTTACATC
CAAATGAAGATGCTCAACACATCGAAAGGTCCGGCGGTACGTGCATTACGTGCACAGGCGGACAAATTCCAATACCAAAA
TCACATGAAGAAAGTGCTAGAAGACGAACCGAATCTACTGCTTCGTCAGGCGCTAGTCGAACGCTTGTTGATTGAGGATG
GAGAAGTACGTGGCGTGGTAACGAACACAGGTTCAGAATATCGCGCGAAAGCGGTCATCGTTACAACGGGAACGTTCATG
CGCGGTCAAATCATCATCGGTGAATTGGCATATGAAAGTGGCCCGAACAACCAAATGCCTTCGGTCGAGTTATCGAAGCA
TCTAGAAGAGCTCGGTCTCGACTTAGTTCGATTCAAAACGGGAACACCGCCACGAATCGACGGCAAGACAATCGATTACT
CGAAGACGGAGATTCAACCAGGAGACGAGAATCCATCATTCTTCTCGTACACCTCGACGGATTTGAATCCGATCGAACAA
ATCCCATGCTGGCTCACGTACACGAGTGAGCGCACGCACCAATTGATTGATGAGAACTTGCATCGCTCACCGATGTACTC
GGGTGCGATTAAAGGAACGGGACCACGTTATTGCCCATCGATTGAAGATAAGGTCGTTCGCTTTAATGATAAACCGCGTC
ACCAAATCTTCTTGGAGCCAGAAGGTCGCGATACAGAAGAAATCTATGTTCAAGGCTTCTCAACGAGTATGCCAGAAGAC
GTGCAACATCAGATGCTTCGCACAATCCCAGGTCTTGAAAATTCAGAGATGATGCGTCCCGGTTATGCCATCGAGTACGA
TGCAGTCGTACCGACACAGCTTTGGCCAACGCTCGAAACAAAAGCAGTCCCTGGCCTTTTCACAGCGGGACAAATCAACG
GGACGAGCGGGTACGAAGAAGCGGCTGGACAGGGCATCATGGCGGGAATCAACGCCGCATGCAAAGTGCTCGGAAAAGAT
CCGGTCATCTTGGACCGTTCGCAAGCGTACATCGGCGTCTTAATCGATGACCTCGTGACGAAAGGGACGAACGAACCGTA
TCGCCTTCTCACATCACGTGCCGAATACCGTCTTTTGTTGCGTCATGACAATGCGGACTTGCGACTCAGCGACATCGGGT
ATGAGATCGGCTTACTTTCAGAAGATCGTCACACTCACCTCAACGAGAAGCGTGTAGCGATTGAAAAAGAAGTGAAGCGA
CTTGCGTCACTTGTCGTCAAACCGACACCTGAAGTCAATGCGATGCTCGAATCGGTCGGTGCGCACCCGCTTAAAGAAGC
GACGCATGCCAACATCTTGTTGAAACGTCCGGAAGTATCTTATGAAGCACTCATGACGGTCGTTCCGTCAACGGAAGAAT
TGTCGTATGAAGTGAAAGAGCAAGTCGAGATTCAAGTGAAGTATGAAGGTTATATTTCCAAACAGCTTGAACAAGTCGAG
AAGATGCGTCGCATGGAAGAAAAACGAATCCCGGAAAACTTGGATTACGATGCGATCGGTGGTCTCGCGACAGAAGCGAA
ACAAAAACTAAAACAAGTTCGTCCTCTTTCGATCGGTCAAGCGTCTCGTATCTCAGGCGTAAACCCAGCAGACGTGTCAA
TCTTGCTCGTCTATATCGAACAAGGACAATACGCAACGGTGGAGTGA

Upstream 100 bases:

>100_bases
GGATACGCTCGGTGAAATCAATGGAGATACCGTACAGGAGAGCTTGATTGATCAGCTCTTTTCACAGTTTTGTCTTGGGA
AATAATAAGGAGGAACGAAT

Downstream 100 bases:

>100_bases
AACGATGAACGAATCTCAATTTATTGAAGCGTTACGAGCTGAAGGGTTTGAACTGAGTGAGACACAACTCAGTCAGTTCA
AACGTTACTTCGAAATGCTC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MAYTAGEFDVIVIGAGHAGIEAALASARMGAKTAMLTMNPDMVGFMYCNPSIGGPAKGIVVREIDALGGEMGRAIDATYI
QMKMLNTSKGPAVRALRAQADKFQYQNHMKKVLEDEPNLLLRQALVERLLIEDGEVRGVVTNTGSEYRAKAVIVTTGTFM
RGQIIIGELAYESGPNNQMPSVELSKHLEELGLDLVRFKTGTPPRIDGKTIDYSKTEIQPGDENPSFFSYTSTDLNPIEQ
IPCWLTYTSERTHQLIDENLHRSPMYSGAIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRDTEEIYVQGFSTSMPED
VQHQMLRTIPGLENSEMMRPGYAIEYDAVVPTQLWPTLETKAVPGLFTAGQINGTSGYEEAAGQGIMAGINAACKVLGKD
PVILDRSQAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLSDIGYEIGLLSEDRHTHLNEKRVAIEKEVKR
LASLVVKPTPEVNAMLESVGAHPLKEATHANILLKRPEVSYEALMTVVPSTEELSYEVKEQVEIQVKYEGYISKQLEQVE
KMRRMEEKRIPENLDYDAIGGLATEAKQKLKQVRPLSIGQASRISGVNPADVSILLVYIEQGQYATVE

Sequences:

>Translated_628_residues
MAYTAGEFDVIVIGAGHAGIEAALASARMGAKTAMLTMNPDMVGFMYCNPSIGGPAKGIVVREIDALGGEMGRAIDATYI
QMKMLNTSKGPAVRALRAQADKFQYQNHMKKVLEDEPNLLLRQALVERLLIEDGEVRGVVTNTGSEYRAKAVIVTTGTFM
RGQIIIGELAYESGPNNQMPSVELSKHLEELGLDLVRFKTGTPPRIDGKTIDYSKTEIQPGDENPSFFSYTSTDLNPIEQ
IPCWLTYTSERTHQLIDENLHRSPMYSGAIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRDTEEIYVQGFSTSMPED
VQHQMLRTIPGLENSEMMRPGYAIEYDAVVPTQLWPTLETKAVPGLFTAGQINGTSGYEEAAGQGIMAGINAACKVLGKD
PVILDRSQAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLSDIGYEIGLLSEDRHTHLNEKRVAIEKEVKR
LASLVVKPTPEVNAMLESVGAHPLKEATHANILLKRPEVSYEALMTVVPSTEELSYEVKEQVEIQVKYEGYISKQLEQVE
KMRRMEEKRIPENLDYDAIGGLATEAKQKLKQVRPLSIGQASRISGVNPADVSILLVYIEQGQYATVE
>Mature_627_residues
AYTAGEFDVIVIGAGHAGIEAALASARMGAKTAMLTMNPDMVGFMYCNPSIGGPAKGIVVREIDALGGEMGRAIDATYIQ
MKMLNTSKGPAVRALRAQADKFQYQNHMKKVLEDEPNLLLRQALVERLLIEDGEVRGVVTNTGSEYRAKAVIVTTGTFMR
GQIIIGELAYESGPNNQMPSVELSKHLEELGLDLVRFKTGTPPRIDGKTIDYSKTEIQPGDENPSFFSYTSTDLNPIEQI
PCWLTYTSERTHQLIDENLHRSPMYSGAIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRDTEEIYVQGFSTSMPEDV
QHQMLRTIPGLENSEMMRPGYAIEYDAVVPTQLWPTLETKAVPGLFTAGQINGTSGYEEAAGQGIMAGINAACKVLGKDP
VILDRSQAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLSDIGYEIGLLSEDRHTHLNEKRVAIEKEVKRL
ASLVVKPTPEVNAMLESVGAHPLKEATHANILLKRPEVSYEALMTVVPSTEELSYEVKEQVEIQVKYEGYISKQLEQVEK
MRRMEEKRIPENLDYDAIGGLATEAKQKLKQVRPLSIGQASRISGVNPADVSILLVYIEQGQYATVE

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=641, Percent_Identity=42.9017160686427, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI19882217, Length=667, Percent_Identity=41.2293853073463, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI183227703, Length=682, Percent_Identity=40.3225806451613, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=53.9837398373984, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=626, Percent_Identity=38.9776357827476, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321202, Length=618, Percent_Identity=43.5275080906149, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24658174, Length=643, Percent_Identity=42.3017107309487, Blast_Score=469, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69650; Mature: 69518

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTAGEFDVIVIGAGHAGIEAALASARMGAKTAMLTMNPDMVGFMYCNPSIGGPAKGIV
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCEE
VREIDALGGEMGRAIDATYIQMKMLNTSKGPAVRALRAQADKFQYQNHMKKVLEDEPNLL
EEEHHHHCCHHCCHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRQALVERLLIEDGEVRGVVTNTGSEYRAKAVIVTTGTFMRGQIIIGELAYESGPNNQMP
HHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCEEECEEEEEEEECCCCCCCCCC
SVELSKHLEELGLDLVRFKTGTPPRIDGKTIDYSKTEIQPGDENPSFFSYTSTDLNPIEQ
CHHHHHHHHHHCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCCCCHHHH
IPCWLTYTSERTHQLIDENLHRSPMYSGAIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEP
CCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECC
EGRDTEEIYVQGFSTSMPEDVQHQMLRTIPGLENSEMMRPGYAIEYDAVVPTQLWPTLET
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCC
KAVPGLFTAGQINGTSGYEEAAGQGIMAGINAACKVLGKDPVILDRSQAYIGVLIDDLVT
CCCCCEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHH
KGTNEPYRLLTSRAEYRLLLRHDNADLRLSDIGYEIGLLSEDRHTHLNEKRVAIEKEVKR
CCCCCCHHHHHCCCCEEEEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHH
LASLVVKPTPEVNAMLESVGAHPLKEATHANILLKRPEVSYEALMTVVPSTEELSYEVKE
HHHHHCCCCCHHHHHHHHHCCCCHHHCCCCEEEEECCCCCHHHHEEECCCHHHHHHEECC
QVEIQVKYEGYISKQLEQVEKMRRMEEKRIPENLDYDAIGGLATEAKQKLKQVRPLSIGQ
CEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCCC
ASRISGVNPADVSILLVYIEQGQYATVE
CHHCCCCCCCCEEEEEEEEECCCEEECC
>Mature Secondary Structure 
AYTAGEFDVIVIGAGHAGIEAALASARMGAKTAMLTMNPDMVGFMYCNPSIGGPAKGIV
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCEE
VREIDALGGEMGRAIDATYIQMKMLNTSKGPAVRALRAQADKFQYQNHMKKVLEDEPNLL
EEEHHHHCCHHCCHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRQALVERLLIEDGEVRGVVTNTGSEYRAKAVIVTTGTFMRGQIIIGELAYESGPNNQMP
HHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCEEECEEEEEEEECCCCCCCCCC
SVELSKHLEELGLDLVRFKTGTPPRIDGKTIDYSKTEIQPGDENPSFFSYTSTDLNPIEQ
CHHHHHHHHHHCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCCCCHHHH
IPCWLTYTSERTHQLIDENLHRSPMYSGAIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEP
CCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECC
EGRDTEEIYVQGFSTSMPEDVQHQMLRTIPGLENSEMMRPGYAIEYDAVVPTQLWPTLET
CCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCC
KAVPGLFTAGQINGTSGYEEAAGQGIMAGINAACKVLGKDPVILDRSQAYIGVLIDDLVT
CCCCCEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCEEHHHHHHHHH
KGTNEPYRLLTSRAEYRLLLRHDNADLRLSDIGYEIGLLSEDRHTHLNEKRVAIEKEVKR
CCCCCCHHHHHCCCCEEEEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHH
LASLVVKPTPEVNAMLESVGAHPLKEATHANILLKRPEVSYEALMTVVPSTEELSYEVKE
HHHHHCCCCCHHHHHHHHHCCCCHHHCCCCEEEEECCCCCHHHHEEECCCHHHHHHEECC
QVEIQVKYEGYISKQLEQVEKMRRMEEKRIPENLDYDAIGGLATEAKQKLKQVRPLSIGQ
CEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCCC
ASRISGVNPADVSILLVYIEQGQYATVE
CHHCCCCCCCCEEEEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA