Click here to switch to the map view.

The map label for this gene is mnmE [H]

Identifier: 229917461

GI number: 229917461

Start: 1704442

End: 1705821

Strand: Direct

Name: mnmE [H]

Synonym: EAT1b_1736

Alternate gene names: 229917461

Gene position: 1704442-1705821 (Clockwise)

Preceding gene: 229917459

Following gene: 229917462

Centisome position: 56.82

GC content: 49.42

Gene sequence:

>1380_bases
ATGCTAGAGTTTGACACGATTACAGCGATTTCGACGCCGATGGGTGAAGGTGCCATCGGGATCGTCCGATTATCGGGCGA
TTTGGCGGTCGAAACGGTGAACAATGTGTTTTCTGGAAAAGACTTAACAACAGTCGATTCACATACGATTCATTATGGAA
CGTTAAAATATCCTGATACGGGCGAAATCGTCGATGAAGTTATGGTCAGTATTATGCGCGCACCGAAGACGTTTACACGA
GAAGATGTCGTCGAGATCAATTGTCACGGAGGCGTCGTGGCGGTCAATCGTGTCCTTGAGCTCATTCTCGATCAACCCGA
CATCCGTTTGGCAGAACCGGGCGAGTTCACGAAGCGCGCCTTTTTAAATGGCCGAATCGATTTATCTCAGGCGGAAGCGG
TCATGGATTTGATTCGCGCGAAGACGGACCGGGCCATGAACGTTGCGATGGCGCAAATGGAAGGACGTTTGTCACGCCTC
GTTCGCGAGTTACGTCAACAGTTGCTCGAGACGATTGCGGCGGTCGAGGTCAACATCGACTATCCAGAGTATGACGCAGA
AGAGATGACCCAGGCCATCGTCGAAGAGAAAGCGGGCGAGGTCAAGCGTGTCCTCGAGCAGTTACTCGAGACAGCGCGAC
AAGGGAAAATCTTACGTGAAGGCTTGGCTACAGCAATCATCGGTCGACCGAACGTCGGTAAATCTTCTCTGATGAATACG
CTCGTTCAAGAGACGAAAGCAATCGTCACCGAAATTGCCGGGACAACACGTGACACGATTGAGGAGTACGTCAATGTGAA
AGGTGTTCCGCTCAAATTGATCGACACGGCAGGGATTCGCGAAACGGAAGATATCGTGGAACGAATCGGTGTGGAAAAGT
CGAGAAAAGCGTTAGAGGCGGCAGATTTGGTCCTTCTCGTCTTGAACGGACACGAGGGATTGACTGTCGAGGATGAGGCG
TTATTTGAGGCAGTAAAAGGTATGAATGCCATCATCATCATCAACAAGACGGACTTGCCACAAAACATGGATTCTTCTAA
GCTAGAGAAACTAGCGGCAGGACGTCCGATTGTGGCGACATCGCTCTTACTAGATGAAGGAATCGATGCCCTTGAAGCGG
CCATCGCAAATCTCTTCTTCGCAAAAGGGGTCGAGTCACAAGATATGACGTATATCTCAAACGCTCGACACATTCAATTA
ATCAAACGAACGATTGGTCAAATTGATGAGGCGCTCGGGAGTGCCGTCATGAATATGCCGATCGATATGGTTCAGATCGA
TTTACGTCGTGCGTGGGATACGCTCGGTGAAATCAATGGAGATACCGTACAGGAGAGCTTGATTGATCAGCTCTTTTCAC
AGTTTTGTCTTGGGAAATAA

Upstream 100 bases:

>100_bases
TTGATCGATGGGCCAACTCTTTCGTTCAAGTCGAATATCGTGTATAGTAGGTTCGTTATGCATATGATAGAAGAAGATGT
TTAGGTTTGGAGGGAGAAAG

Downstream 100 bases:

>100_bases
TAAGGAGGAACGAATATGGCTTATACGGCAGGCGAATTTGACGTCATCGTCATCGGGGCCGGACACGCAGGGATTGAAGC
AGCACTTGCATCGGCTCGCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLEFDTITAISTPMGEGAIGIVRLSGDLAVETVNNVFSGKDLTTVDSHTIHYGTLKYPDTGEIVDEVMVSIMRAPKTFTR
EDVVEINCHGGVVAVNRVLELILDQPDIRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMAQMEGRLSRL
VRELRQQLLETIAAVEVNIDYPEYDAEEMTQAIVEEKAGEVKRVLEQLLETARQGKILREGLATAIIGRPNVGKSSLMNT
LVQETKAIVTEIAGTTRDTIEEYVNVKGVPLKLIDTAGIRETEDIVERIGVEKSRKALEAADLVLLVLNGHEGLTVEDEA
LFEAVKGMNAIIIINKTDLPQNMDSSKLEKLAAGRPIVATSLLLDEGIDALEAAIANLFFAKGVESQDMTYISNARHIQL
IKRTIGQIDEALGSAVMNMPIDMVQIDLRRAWDTLGEINGDTVQESLIDQLFSQFCLGK

Sequences:

>Translated_459_residues
MLEFDTITAISTPMGEGAIGIVRLSGDLAVETVNNVFSGKDLTTVDSHTIHYGTLKYPDTGEIVDEVMVSIMRAPKTFTR
EDVVEINCHGGVVAVNRVLELILDQPDIRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMAQMEGRLSRL
VRELRQQLLETIAAVEVNIDYPEYDAEEMTQAIVEEKAGEVKRVLEQLLETARQGKILREGLATAIIGRPNVGKSSLMNT
LVQETKAIVTEIAGTTRDTIEEYVNVKGVPLKLIDTAGIRETEDIVERIGVEKSRKALEAADLVLLVLNGHEGLTVEDEA
LFEAVKGMNAIIIINKTDLPQNMDSSKLEKLAAGRPIVATSLLLDEGIDALEAAIANLFFAKGVESQDMTYISNARHIQL
IKRTIGQIDEALGSAVMNMPIDMVQIDLRRAWDTLGEINGDTVQESLIDQLFSQFCLGK
>Mature_459_residues
MLEFDTITAISTPMGEGAIGIVRLSGDLAVETVNNVFSGKDLTTVDSHTIHYGTLKYPDTGEIVDEVMVSIMRAPKTFTR
EDVVEINCHGGVVAVNRVLELILDQPDIRLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMAQMEGRLSRL
VRELRQQLLETIAAVEVNIDYPEYDAEEMTQAIVEEKAGEVKRVLEQLLETARQGKILREGLATAIIGRPNVGKSSLMNT
LVQETKAIVTEIAGTTRDTIEEYVNVKGVPLKLIDTAGIRETEDIVERIGVEKSRKALEAADLVLLVLNGHEGLTVEDEA
LFEAVKGMNAIIIINKTDLPQNMDSSKLEKLAAGRPIVATSLLLDEGIDALEAAIANLFFAKGVESQDMTYISNARHIQL
IKRTIGQIDEALGSAVMNMPIDMVQIDLRRAWDTLGEINGDTVQESLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=33.6170212765957, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI193082993, Length=458, Percent_Identity=32.7510917030568, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=30.8764940239044, Blast_Score=205, Evalue=6e-53,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=34.7639484978541, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI87082120, Length=119, Percent_Identity=40.3361344537815, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=29.8924731182796, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=489, Percent_Identity=29.6523517382413, Blast_Score=207, Evalue=4e-54,
Organism=Drosophila melanogaster, GI45550813, Length=479, Percent_Identity=30.6889352818372, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50385; Mature: 50385

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFDTITAISTPMGEGAIGIVRLSGDLAVETVNNVFSGKDLTTVDSHTIHYGTLKYPDT
CCCCCCCHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCCEEEECCCCCCCC
GEIVDEVMVSIMRAPKTFTREDVVEINCHGGVVAVNRVLELILDQPDIRLAEPGEFTKRA
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHH
FLNGRIDLSQAEAVMDLIRAKTDRAMNVAMAQMEGRLSRLVRELRQQLLETIAAVEVNID
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
YPEYDAEEMTQAIVEEKAGEVKRVLEQLLETARQGKILREGLATAIIGRPNVGKSSLMNT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
LVQETKAIVTEIAGTTRDTIEEYVNVKGVPLKLIDTAGIRETEDIVERIGVEKSRKALEA
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHH
ADLVLLVLNGHEGLTVEDEALFEAVKGMNAIIIINKTDLPQNMDSSKLEKLAAGRPIVAT
HCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
SLLLDEGIDALEAAIANLFFAKGVESQDMTYISNARHIQLIKRTIGQIDEALGSAVMNMP
HHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IDMVQIDLRRAWDTLGEINGDTVQESLIDQLFSQFCLGK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEFDTITAISTPMGEGAIGIVRLSGDLAVETVNNVFSGKDLTTVDSHTIHYGTLKYPDT
CCCCCCCHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCCEEEECCCCCCCC
GEIVDEVMVSIMRAPKTFTREDVVEINCHGGVVAVNRVLELILDQPDIRLAEPGEFTKRA
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEECCCCHHHHHH
FLNGRIDLSQAEAVMDLIRAKTDRAMNVAMAQMEGRLSRLVRELRQQLLETIAAVEVNID
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
YPEYDAEEMTQAIVEEKAGEVKRVLEQLLETARQGKILREGLATAIIGRPNVGKSSLMNT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
LVQETKAIVTEIAGTTRDTIEEYVNVKGVPLKLIDTAGIRETEDIVERIGVEKSRKALEA
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHH
ADLVLLVLNGHEGLTVEDEALFEAVKGMNAIIIINKTDLPQNMDSSKLEKLAAGRPIVAT
HCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
SLLLDEGIDALEAAIANLFFAKGVESQDMTYISNARHIQLIKRTIGQIDEALGSAVMNMP
HHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IDMVQIDLRRAWDTLGEINGDTVQESLIDQLFSQFCLGK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA