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The map label for this gene is rsmG

Identifier: 229917463

GI number: 229917463

Start: 1707728

End: 1708444

Strand: Direct

Name: rsmG

Synonym: EAT1b_1738

Alternate gene names: 229917463

Gene position: 1707728-1708444 (Clockwise)

Preceding gene: 229917462

Following gene: 229917464

Centisome position: 56.93

GC content: 48.81

Gene sequence:

>717_bases
ATGAACGAATCTCAATTTATTGAAGCGTTACGAGCTGAAGGGTTTGAACTGAGTGAGACACAACTCAGTCAGTTCAAACG
TTACTTCGAAATGCTCGTCGAATGGAACGAAAAAATGAACTTAACGGCCATCACGGACCGGGAAGAAGTATATTTGAAGC
ACTTCTATGATTCGCTCAGCGCAGCGTTTCATTTCGATTTCACGAAAGTCGAAACCGTATGTGATGTCGGGGCAGGAGCG
GGATTCCCGAGTCTTCCGCTCAAAATTTTATTCCCACACTTACACGTCACGATCATCGATTCGTTAAACAAACGCATCAC
CTTCTTGAACGAATTGGCCCTCGCCCTCGGGCTCGAAGGTGTCGCGTTCCATCATGGTCGCGCTGAAGAGTTCGGGAAAA
ATAAAAAGTTCCGCGAACAGTTTGACGTCGTCACAGCGCGTGCGGTTGCCCGCATGACCGTGTTGGCGGAATACTGTTTG
CCTCTCGCGAAAGTAGGCGGAAGCTTCGTCGCGCTTAAAGCAGCCAAGGTTTCTGACGAGATGGCAGACGCCAAAAACGC
TTTAGCCGTTCTAGGTGGAAAAGAAAAAGCGACACACCAGTTCCTATTGCCAGGAGAGATGAGTGAGCGTAATATCGTAG
TTGTAGATAAACTACGAAAAACGCCCGGGAAATATCCGCGTAAAGCGGGGACACCGGCGAAAGAGCCACTTATCTGA

Upstream 100 bases:

>100_bases
ATCGGTCAAGCGTCTCGTATCTCAGGCGTAAACCCAGCAGACGTGTCAATCTTGCTCGTCTATATCGAACAAGGACAATA
CGCAACGGTGGAGTGAAACG

Downstream 100 bases:

>100_bases
TGATGTCGTTTCACGTGAAACAAAAGCAGATTCCAGTGGTGGAGTCTGCTTATTTTTTTACATAAAGGGGTGCCCGACGT
TGAAGAATAGATTTGCGAAA

Product: methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNESQFIEALRAEGFELSETQLSQFKRYFEMLVEWNEKMNLTAITDREEVYLKHFYDSLSAAFHFDFTKVETVCDVGAGA
GFPSLPLKILFPHLHVTIIDSLNKRITFLNELALALGLEGVAFHHGRAEEFGKNKKFREQFDVVTARAVARMTVLAEYCL
PLAKVGGSFVALKAAKVSDEMADAKNALAVLGGKEKATHQFLLPGEMSERNIVVVDKLRKTPGKYPRKAGTPAKEPLI

Sequences:

>Translated_238_residues
MNESQFIEALRAEGFELSETQLSQFKRYFEMLVEWNEKMNLTAITDREEVYLKHFYDSLSAAFHFDFTKVETVCDVGAGA
GFPSLPLKILFPHLHVTIIDSLNKRITFLNELALALGLEGVAFHHGRAEEFGKNKKFREQFDVVTARAVARMTVLAEYCL
PLAKVGGSFVALKAAKVSDEMADAKNALAVLGGKEKATHQFLLPGEMSERNIVVVDKLRKTPGKYPRKAGTPAKEPLI
>Mature_238_residues
MNESQFIEALRAEGFELSETQLSQFKRYFEMLVEWNEKMNLTAITDREEVYLKHFYDSLSAAFHFDFTKVETVCDVGAGA
GFPSLPLKILFPHLHVTIIDSLNKRITFLNELALALGLEGVAFHHGRAEEFGKNKKFREQFDVVTARAVARMTVLAEYCL
PLAKVGGSFVALKAAKVSDEMADAKNALAVLGGKEKATHQFLLPGEMSERNIVVVDKLRKTPGKYPRKAGTPAKEPLI

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=206, Percent_Identity=33.0097087378641, Blast_Score=111, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_EXISA (C4L001)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886109.1
- ProteinModelPortal:   C4L001
- GeneID:   7870785
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1738
- OMA:   HIMDSIV
- ProtClustDB:   CLSK2490038
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 26594; Mature: 26594

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESQFIEALRAEGFELSETQLSQFKRYFEMLVEWNEKMNLTAITDREEVYLKHFYDSLS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
AAFHFDFTKVETVCDVGAGAGFPSLPLKILFPHLHVTIIDSLNKRITFLNELALALGLEG
HHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VAFHHGRAEEFGKNKKFREQFDVVTARAVARMTVLAEYCLPLAKVGGSFVALKAAKVSDE
CEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
MADAKNALAVLGGKEKATHQFLLPGEMSERNIVVVDKLRKTPGKYPRKAGTPAKEPLI
HHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNESQFIEALRAEGFELSETQLSQFKRYFEMLVEWNEKMNLTAITDREEVYLKHFYDSLS
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
AAFHFDFTKVETVCDVGAGAGFPSLPLKILFPHLHVTIIDSLNKRITFLNELALALGLEG
HHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VAFHHGRAEEFGKNKKFREQFDVVTARAVARMTVLAEYCLPLAKVGGSFVALKAAKVSDE
CEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
MADAKNALAVLGGKEKATHQFLLPGEMSERNIVVVDKLRKTPGKYPRKAGTPAKEPLI
HHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA