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The map label for this gene is tmk

Identifier: 229917431

GI number: 229917431

Start: 1654581

End: 1655219

Strand: Reverse

Name: tmk

Synonym: EAT1b_1706

Alternate gene names: 229917431

Gene position: 1655219-1654581 (Counterclockwise)

Preceding gene: 229917432

Following gene: 229917430

Centisome position: 55.18

GC content: 45.7

Gene sequence:

>639_bases
GTGAGCGGAATGTTTATTACGGTTGAAGGACCTGATGGATCAGGAAAGTCCACACAACTTCAATTATTAGTAGAAAACTT
AAAACAAAAAGGATATGACATCGTCGTGACCCGAGAACCTGGAGGAACAACGGTCGGGAATCAGATTCGCGAAGTGTTAT
TGTCTCCCGATCATCATGAGATGACACCACGTGTGGAGATGATGCTCTATGCCGCATCACGGGCACAGAACGTGGAACAA
GTGATTCGGCCAGCGTTAGAGCGGGGCGCCATTGTTCTCTGCGACCGGTTCATCGATGCATCCATTGCTTATCAAGGATA
TGGATTGCAGTATGACCTTGATCAGATTCGGTCATTAAATGAATGGGCGACGAATGGTCTCACGCCCGATTTAACCTTTT
TATTCGATTTAAATCCGGAACGGGCGAGTGCACGTATGAAAGATCGAGGACAGCTTGATCGAATCGAGAGTCGTGATCAA
GCATTCCATGAGCGTGTCTATGCAGGATTCCAAACGTTACTGAAACAGTATCCAGAACGCATCGTTCGGATTGATGCCGA
TCAATCGATTGAATGCATTCAAGATGAGGTACTTGACTATACAATTGAACGACTACAACAAGGAGGCGTACAAAAATGA

Upstream 100 bases:

>100_bases
CCGTGTGCTCCGGGAATGGATTTCTTTAGGGAGGAAGCTTCAAGCTAGTAAGCATGTTATGATAGAGCAAATTGACGTTA
TTGTAGAGGAGTTTTAAAAC

Downstream 100 bases:

>100_bases
TGAAAATGGTAATTGCAATCGTACATGATAAGGATAGCGCACGTTTGTCCGACGCACTCGTCGAGCAAGATTTCCGCGCG
ACAAAGTTAGCCACGACGGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSGMFITVEGPDGSGKSTQLQLLVENLKQKGYDIVVTREPGGTTVGNQIREVLLSPDHHEMTPRVEMMLYAASRAQNVEQ
VIRPALERGAIVLCDRFIDASIAYQGYGLQYDLDQIRSLNEWATNGLTPDLTFLFDLNPERASARMKDRGQLDRIESRDQ
AFHERVYAGFQTLLKQYPERIVRIDADQSIECIQDEVLDYTIERLQQGGVQK

Sequences:

>Translated_212_residues
MSGMFITVEGPDGSGKSTQLQLLVENLKQKGYDIVVTREPGGTTVGNQIREVLLSPDHHEMTPRVEMMLYAASRAQNVEQ
VIRPALERGAIVLCDRFIDASIAYQGYGLQYDLDQIRSLNEWATNGLTPDLTFLFDLNPERASARMKDRGQLDRIESRDQ
AFHERVYAGFQTLLKQYPERIVRIDADQSIECIQDEVLDYTIERLQQGGVQK
>Mature_211_residues
SGMFITVEGPDGSGKSTQLQLLVENLKQKGYDIVVTREPGGTTVGNQIREVLLSPDHHEMTPRVEMMLYAASRAQNVEQV
IRPALERGAIVLCDRFIDASIAYQGYGLQYDLDQIRSLNEWATNGLTPDLTFLFDLNPERASARMKDRGQLDRIESRDQA
FHERVYAGFQTLLKQYPERIVRIDADQSIECIQDEVLDYTIERLQQGGVQK

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=201, Percent_Identity=37.3134328358209, Blast_Score=123, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_EXISA (C4KZW9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886077.1
- GeneID:   7869772
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1706
- OMA:   LFITLEG
- ProtClustDB:   CLSK2488823
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 24066; Mature: 23934

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGMFITVEGPDGSGKSTQLQLLVENLKQKGYDIVVTREPGGTTVGNQIREVLLSPDHHE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
MTPRVEMMLYAASRAQNVEQVIRPALERGAIVLCDRFIDASIAYQGYGLQYDLDQIRSLN
CCHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHEECCCCCEECHHHHHHHH
EWATNGLTPDLTFLFDLNPERASARMKDRGQLDRIESRDQAFHERVYAGFQTLLKQYPER
HHHHCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVRIDADQSIECIQDEVLDYTIERLQQGGVQK
HEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGMFITVEGPDGSGKSTQLQLLVENLKQKGYDIVVTREPGGTTVGNQIREVLLSPDHHE
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCC
MTPRVEMMLYAASRAQNVEQVIRPALERGAIVLCDRFIDASIAYQGYGLQYDLDQIRSLN
CCHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHEECCCCCEECHHHHHHHH
EWATNGLTPDLTFLFDLNPERASARMKDRGQLDRIESRDQAFHERVYAGFQTLLKQYPER
HHHHCCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVRIDADQSIECIQDEVLDYTIERLQQGGVQK
HEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA