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The map label for this gene is yaaO [H]

Identifier: 229917432

GI number: 229917432

Start: 1655223

End: 1656641

Strand: Reverse

Name: yaaO [H]

Synonym: EAT1b_1707

Alternate gene names: 229917432

Gene position: 1656641-1655223 (Counterclockwise)

Preceding gene: 229917433

Following gene: 229917431

Centisome position: 55.22

GC content: 44.82

Gene sequence:

>1419_bases
ATGGCTCCTGCTCGGGAAAATCCATTTACATTTTTAGAGGTTCTAGAACAGCATGTTCGTGAACACCGCGTCTCTTTCCA
CGTACCAGGTCATCATAACGGTACAGTGGTTGATGATCGCTTACCTGATGCATTCGCAAAGATCATGCCTTACGACTTGA
CCGAGCTAGCGCATTTAGATGATTTGCACGATGCCTCGGGAATCATCGAGCAAGCTGAACGTGCGTTGGGTAATCTATAC
GCGAGTAACAGTAAGTGGCTTGTCAACGGATCGACTGTGGGAAATTGGAGTATGCTCGCGGCAACAGTGAAACGTGGTGA
ACGGGTATATGTTCAACGGAACTCACATAAATCGGTTTGGAACGCAATCGAGTGGTTAGGATTAAAGCCAGTGTTACTAA
CACCTACCATTGAACGTGGCACAGGCATCCCGACTGTCGTGACATTAGATACGATCGAACAGGCCATTAAAGAATTTCCT
GGAGGGAAAGCCGTCATGTTGACGTCTCCGACCTATTATGGGGATATGGCAGACATTCGAACGATTTCGGACTATTTGAC
ACCGTTAGACATTCCATTGCTTGTCGATGAAGCACACGGCGCTCATTTAATTCATCCACAGTTGCCACGTCGCTCTGCAA
TTTTCGAAGGAGCGAGTGCAGTCGTGCAGTCCGCTCATAAAACGTTGCCTGCCTTAACCATGGGGGCTTGGGTTCACTAT
AAAGATAATATCGATGAAGCGCGTTTGAAGCATGCATTAGCCTCTTTCCAGACCTCGAGTCCGTCTTATCTCATCATGGC
TTCATTGGATTATGCTCGTTCTTATCTAGAACGTTTAACGGATGAGGAATGGTCTGCTATCCAAAAAAGCCATGCACAAT
TTGTTCTTGAATTAAAACGAGCTGGTTTTGTTGTAAAAGTCGGAGATGACTTTATGAAAGTGACGATTCTGACTTATCCA
AAGAATGGATTGATCTTTAGTCAGCATCTCGCCTCAATCGGCATAGACATCGAGTTGGCGGCAGTCGATCATGTGTTATT
CTGCTTGCCATTGAGGGAAATAAAGAAACAGGAAATCGAACATTGGATCAAATTAATAAAAAAAGCATCTACCCATGTGC
CGATCATTGAAGACGATGAAACGATCTTTCCCTTCCCACAAACCGAGCGCGTGTCTGAACTCTATACGTCAGAAACAGCT
CTTCCAATGCAGCGAGTTCTGTTCGACCAAGCAATCGGATTGATTTCAGCAGAGACGGTGATTCCATATCCACCAGGTAT
TCCATGGATTCTAAAAGGAGAGCGGATTACAGAAGAAAAAATCCGTGTGCTCCGGGAATGGATTTCTTTAGGGAGGAAGC
TTCAAGCTAGTAAGCATGTTATGATAGAGCAAATTGACGTTATTGTAGAGGAGTTTTAA

Upstream 100 bases:

>100_bases
CCCCTGTGAGGATAGGACGTTGCCAGGCCAACGCAGAAGACGATCAAGCATATGCTTGATCGTCTTTTTTCATATTTTGA
TAGTTTAGGAGGAATGACAG

Downstream 100 bases:

>100_bases
AACGTGAGCGGAATGTTTATTACGGTTGAAGGACCTGATGGATCAGGAAAGTCCACACAACTTCAATTATTAGTAGAAAA
CTTAAAACAAAAAGGATATG

Product: Orn/Lys/Arg decarboxylase major region

Products: 1,5-Diaminopentane; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAPARENPFTFLEVLEQHVREHRVSFHVPGHHNGTVVDDRLPDAFAKIMPYDLTELAHLDDLHDASGIIEQAERALGNLY
ASNSKWLVNGSTVGNWSMLAATVKRGERVYVQRNSHKSVWNAIEWLGLKPVLLTPTIERGTGIPTVVTLDTIEQAIKEFP
GGKAVMLTSPTYYGDMADIRTISDYLTPLDIPLLVDEAHGAHLIHPQLPRRSAIFEGASAVVQSAHKTLPALTMGAWVHY
KDNIDEARLKHALASFQTSSPSYLIMASLDYARSYLERLTDEEWSAIQKSHAQFVLELKRAGFVVKVGDDFMKVTILTYP
KNGLIFSQHLASIGIDIELAAVDHVLFCLPLREIKKQEIEHWIKLIKKASTHVPIIEDDETIFPFPQTERVSELYTSETA
LPMQRVLFDQAIGLISAETVIPYPPGIPWILKGERITEEKIRVLREWISLGRKLQASKHVMIEQIDVIVEEF

Sequences:

>Translated_472_residues
MAPARENPFTFLEVLEQHVREHRVSFHVPGHHNGTVVDDRLPDAFAKIMPYDLTELAHLDDLHDASGIIEQAERALGNLY
ASNSKWLVNGSTVGNWSMLAATVKRGERVYVQRNSHKSVWNAIEWLGLKPVLLTPTIERGTGIPTVVTLDTIEQAIKEFP
GGKAVMLTSPTYYGDMADIRTISDYLTPLDIPLLVDEAHGAHLIHPQLPRRSAIFEGASAVVQSAHKTLPALTMGAWVHY
KDNIDEARLKHALASFQTSSPSYLIMASLDYARSYLERLTDEEWSAIQKSHAQFVLELKRAGFVVKVGDDFMKVTILTYP
KNGLIFSQHLASIGIDIELAAVDHVLFCLPLREIKKQEIEHWIKLIKKASTHVPIIEDDETIFPFPQTERVSELYTSETA
LPMQRVLFDQAIGLISAETVIPYPPGIPWILKGERITEEKIRVLREWISLGRKLQASKHVMIEQIDVIVEEF
>Mature_471_residues
APARENPFTFLEVLEQHVREHRVSFHVPGHHNGTVVDDRLPDAFAKIMPYDLTELAHLDDLHDASGIIEQAERALGNLYA
SNSKWLVNGSTVGNWSMLAATVKRGERVYVQRNSHKSVWNAIEWLGLKPVLLTPTIERGTGIPTVVTLDTIEQAIKEFPG
GKAVMLTSPTYYGDMADIRTISDYLTPLDIPLLVDEAHGAHLIHPQLPRRSAIFEGASAVVQSAHKTLPALTMGAWVHYK
DNIDEARLKHALASFQTSSPSYLIMASLDYARSYLERLTDEEWSAIQKSHAQFVLELKRAGFVVKVGDDFMKVTILTYPK
NGLIFSQHLASIGIDIELAAVDHVLFCLPLREIKKQEIEHWIKLIKKASTHVPIIEDDETIFPFPQTERVSELYTSETAL
PMQRVLFDQAIGLISAETVIPYPPGIPWILKGERITEEKIRVLREWISLGRKLQASKHVMIEQIDVIVEEF

Specific function: Appears To Play A Role In pH Homeostasis By Consuming Protons And Neutralizing The Acidic By-Products Of Carbohydrate Fermentation. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1790573, Length=294, Percent_Identity=24.8299319727891, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI221142684, Length=287, Percent_Identity=25.4355400696864, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786384, Length=298, Percent_Identity=27.1812080536913, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18 [C]

Molecular weight: Translated: 53164; Mature: 53033

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPARENPFTFLEVLEQHVREHRVSFHVPGHHNGTVVDDRLPDAFAKIMPYDLTELAHLD
CCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHH
DLHDASGIIEQAERALGNLYASNSKWLVNGSTVGNWSMLAATVKRGERVYVQRNSHKSVW
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCHHHH
NAIEWLGLKPVLLTPTIERGTGIPTVVTLDTIEQAIKEFPGGKAVMLTSPTYYGDMADIR
HHHHHCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
TISDYLTPLDIPLLVDEAHGAHLIHPQLPRRSAIFEGASAVVQSAHKTLPALTMGAWVHY
HHHHHCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCEEEE
KDNIDEARLKHALASFQTSSPSYLIMASLDYARSYLERLTDEEWSAIQKSHAQFVLELKR
CCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AGFVVKVGDDFMKVTILTYPKNGLIFSQHLASIGIDIELAAVDHVLFCLPLREIKKQEIE
CCEEEEECCCEEEEEEEEECCCCCCHHHHHHHHCCCEEEHHHHHHHHHHCHHHHHHHHHH
HWIKLIKKASTHVPIIEDDETIFPFPQTERVSELYTSETALPMQRVLFDQAIGLISAETV
HHHHHHHHCCCCCCEECCCCEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEECCEE
IPYPPGIPWILKGERITEEKIRVLREWISLGRKLQASKHVMIEQIDVIVEEF
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APARENPFTFLEVLEQHVREHRVSFHVPGHHNGTVVDDRLPDAFAKIMPYDLTELAHLD
CCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHH
DLHDASGIIEQAERALGNLYASNSKWLVNGSTVGNWSMLAATVKRGERVYVQRNSHKSVW
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCHHHH
NAIEWLGLKPVLLTPTIERGTGIPTVVTLDTIEQAIKEFPGGKAVMLTSPTYYGDMADIR
HHHHHCCCCCEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
TISDYLTPLDIPLLVDEAHGAHLIHPQLPRRSAIFEGASAVVQSAHKTLPALTMGAWVHY
HHHHHCCCCCCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCEEEE
KDNIDEARLKHALASFQTSSPSYLIMASLDYARSYLERLTDEEWSAIQKSHAQFVLELKR
CCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AGFVVKVGDDFMKVTILTYPKNGLIFSQHLASIGIDIELAAVDHVLFCLPLREIKKQEIE
CCEEEEECCCEEEEEEEEECCCCCCHHHHHHHHCCCEEEHHHHHHHHHHCHHHHHHHHHH
HWIKLIKKASTHVPIIEDDETIFPFPQTERVSELYTSETALPMQRVLFDQAIGLISAETV
HHHHHHHHCCCCCCEECCCCEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHEECCEE
IPYPPGIPWILKGERITEEKIRVLREWISLGRKLQASKHVMIEQIDVIVEEF
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: H+; L-Lysine [C]

Specific reaction: H+ + L-Lysine --> 1,5-Diaminopentane + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]