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The map label for this gene is rbsB [H]

Identifier: 229917240

GI number: 229917240

Start: 1483960

End: 1484871

Strand: Reverse

Name: rbsB [H]

Synonym: EAT1b_1515

Alternate gene names: 229917240

Gene position: 1484871-1483960 (Counterclockwise)

Preceding gene: 229917241

Following gene: 229917239

Centisome position: 49.5

GC content: 51.21

Gene sequence:

>912_bases
ATGAAAAAATGGACAGCTTGGCTACTCGCACTGACTATGGTACTGATGGCAGCTTGCTCGACTGAACAACCGGGGTCATC
GTCTGAAACGGAAACGAAAGATGGCGATTATGAGATCGGATTATCCATCTCGACATTGAACAACCCGTTCTTTGTCGCAT
TGAAAGAAGGCGCGGAAGAACAGGCGAACGAAATGGATGCGACGTTGACGGTAGCGGACGCGCAAAACGACGCGGCGAAG
CAAGTGAATGACGTCGAAGATATGATTCAAAAGGGGATGGATTTGATTCTCATCAACCCGACGGATTCAGAAGCGGTCGG
TGCGGCAGTACAGGCAGCAAACGATGCAGGCATTCCTGTCATCACGGTAGACCGTAACGCTGAAACAGGGGAAGTCGTCG
CACACGTGGCATCAGATAACGTTGCGGGTGGACAATTGGCAGGTGACTACATGGTCGAACTCGTAGGTGAAGGGCAAAAA
GTCGTCGAGCTCGAGGGGATTCCAGGAGCGTCGGCGACACGTGACCGCGGACAAGGATTCAACGAAGCGATCGATGGGAA
ATTAGAAGTCGTGGCAAAACAATCTGCGAACTTTGACCGTGCAGAAGGATTGACGGTCATGGAGAACATCTTACAGGACA
ATAAAGACATCGTCGCGGTATTCGCGCATAACGACGAGATGGCGCTCGGTGCCGTTCAAGCGCTCGAAGCGGCAGGTATG
AGTGATGTCAAAGTCATTGGATTCGATGCGACAGATGATGCGGTCAAAGCAGTCGAAGGTGGGACGATGGCAGCGACGGT
TGCCCAAAAGCCCGCTGAGATTGGTAAATTGGGTGTAGAAGCGGCAATCAACCACCTGAAAGGTGAAACAGTAGAGGAAA
ACATTCCGGTCGAACTCGAATTAATCAAATAA

Upstream 100 bases:

>100_bases
CATCTTTCTTCCAACTCGTCGTAAAAGGTGCAGTTATATTGTTTGCAGTATTGCTTGATCGTCGACAAAACGCGTAACAC
GCGAAAGGGGAAAACGGAAC

Downstream 100 bases:

>100_bases
GTAAGTAGAAACCTCACGATGCCTTGATTTCGGGTAGAGCTCGTGAGGTTTTTGAATGTTCACATATCCCTCTTCATATA
TTGGATAGTTTCTCCCTTTT

Product: LacI family transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKKWTAWLLALTMVLMAACSTEQPGSSSETETKDGDYEIGLSISTLNNPFFVALKEGAEEQANEMDATLTVADAQNDAAK
QVNDVEDMIQKGMDLILINPTDSEAVGAAVQAANDAGIPVITVDRNAETGEVVAHVASDNVAGGQLAGDYMVELVGEGQK
VVELEGIPGASATRDRGQGFNEAIDGKLEVVAKQSANFDRAEGLTVMENILQDNKDIVAVFAHNDEMALGAVQALEAAGM
SDVKVIGFDATDDAVKAVEGGTMAATVAQKPAEIGKLGVEAAINHLKGETVEENIPVELELIK

Sequences:

>Translated_303_residues
MKKWTAWLLALTMVLMAACSTEQPGSSSETETKDGDYEIGLSISTLNNPFFVALKEGAEEQANEMDATLTVADAQNDAAK
QVNDVEDMIQKGMDLILINPTDSEAVGAAVQAANDAGIPVITVDRNAETGEVVAHVASDNVAGGQLAGDYMVELVGEGQK
VVELEGIPGASATRDRGQGFNEAIDGKLEVVAKQSANFDRAEGLTVMENILQDNKDIVAVFAHNDEMALGAVQALEAAGM
SDVKVIGFDATDDAVKAVEGGTMAATVAQKPAEIGKLGVEAAINHLKGETVEENIPVELELIK
>Mature_303_residues
MKKWTAWLLALTMVLMAACSTEQPGSSSETETKDGDYEIGLSISTLNNPFFVALKEGAEEQANEMDATLTVADAQNDAAK
QVNDVEDMIQKGMDLILINPTDSEAVGAAVQAANDAGIPVITVDRNAETGEVVAHVASDNVAGGQLAGDYMVELVGEGQK
VVELEGIPGASATRDRGQGFNEAIDGKLEVVAKQSANFDRAEGLTVMENILQDNKDIVAVFAHNDEMALGAVQALEAAGM
SDVKVIGFDATDDAVKAVEGGTMAATVAQKPAEIGKLGVEAAINHLKGETVEENIPVELELIK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=265, Percent_Identity=55.8490566037736, Blast_Score=296, Evalue=8e-82,
Organism=Escherichia coli, GI1790526, Length=279, Percent_Identity=32.6164874551971, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790674, Length=258, Percent_Identity=32.9457364341085, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1788473, Length=253, Percent_Identity=27.6679841897233, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789990, Length=246, Percent_Identity=29.2682926829268, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790194, Length=284, Percent_Identity=24.2957746478873, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787948, Length=259, Percent_Identity=27.4131274131274, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31714; Mature: 31714

Theoretical pI: Translated: 3.91; Mature: 3.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWTAWLLALTMVLMAACSTEQPGSSSETETKDGDYEIGLSISTLNNPFFVALKEGAEE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCHH
QANEMDATLTVADAQNDAAKQVNDVEDMIQKGMDLILINPTDSEAVGAAVQAANDAGIPV
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEE
ITVDRNAETGEVVAHVASDNVAGGQLAGDYMVELVGEGQKVVELEGIPGASATRDRGQGF
EEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCC
NEAIDGKLEVVAKQSANFDRAEGLTVMENILQDNKDIVAVFAHNDEMALGAVQALEAAGM
CHHHCCEEEEEEECCCCCCHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCC
SDVKVIGFDATDDAVKAVEGGTMAATVAQKPAEIGKLGVEAAINHLKGETVEENIPVELE
CCEEEEEECCCCHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
LIK
EEC
>Mature Secondary Structure
MKKWTAWLLALTMVLMAACSTEQPGSSSETETKDGDYEIGLSISTLNNPFFVALKEGAEE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCHH
QANEMDATLTVADAQNDAAKQVNDVEDMIQKGMDLILINPTDSEAVGAAVQAANDAGIPV
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEE
ITVDRNAETGEVVAHVASDNVAGGQLAGDYMVELVGEGQKVVELEGIPGASATRDRGQGF
EEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCC
NEAIDGKLEVVAKQSANFDRAEGLTVMENILQDNKDIVAVFAHNDEMALGAVQALEAAGM
CHHHCCEEEEEEECCCCCCHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCC
SDVKVIGFDATDDAVKAVEGGTMAATVAQKPAEIGKLGVEAAINHLKGETVEENIPVELE
CCEEEEEECCCCHHHHHHCCCCEEEHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
LIK
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]