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The map label for this gene is ywlF [H]

Identifier: 229917060

GI number: 229917060

Start: 1304960

End: 1305400

Strand: Reverse

Name: ywlF [H]

Synonym: EAT1b_1334

Alternate gene names: 229917060

Gene position: 1305400-1304960 (Counterclockwise)

Preceding gene: 229917061

Following gene: 229917059

Centisome position: 43.51

GC content: 51.93

Gene sequence:

>441_bases
ATGAAAATTGCAATTGGCGCTGATCATGGCGGCTTCAACTTGAAGAAAGAAATCGTAGCCTTGCTTGAGGAACTCGGACA
TGAGTATAAAGATTTCGGGACGCACTCAGCGGACTCGATTGACTACCCGGATGTGGCCATCCCGGTCGCGGAAGCGGTAG
CGGCAGGTGAATTCGATCGTGGGATTTTGATTTGCGGTACGGGAATCGGAATCGGGATTGCGGCGAACAAAGTCAAAGGG
ATTCGTGCGGCACTCGTCCATGATTCATTCAGTGCAAAAGCGACACGTCAGCATAACGATTCGAACATCATGACGATGGG
CGAGCGTGTCATCGGACCTGGTCTTGCACTCGACTTGGTGACGACGTGGCTCGATACGGACTTTGAAGGCGGTCGCCATT
CAAACCGTGTCGACAAGATGAGTGCTTACGAATCGAAGTGA

Upstream 100 bases:

>100_bases
GAACCACTCGTGAATCGACTTGTGTTAAAATTAACGAATGATGGGTCAAAGGTGACGAAACCACCGAGACGCCTACCAAA
GAAGACGGGGGAATGAACGA

Downstream 100 bases:

>100_bases
GTCAAATGAAGACGGAACTTCTCGGATTTTTGACGGAGCTTCACGAACGGTTCCCGTTGAATGATGAGCGCATTCTCGTC
ATCGGATGCTCGACGAGTGA

Product: sugar-phosphate isomerase, RpiB/LacA/LacB family

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKIAIGADHGGFNLKKEIVALLEELGHEYKDFGTHSADSIDYPDVAIPVAEAVAAGEFDRGILICGTGIGIGIAANKVKG
IRAALVHDSFSAKATRQHNDSNIMTMGERVIGPGLALDLVTTWLDTDFEGGRHSNRVDKMSAYESK

Sequences:

>Translated_146_residues
MKIAIGADHGGFNLKKEIVALLEELGHEYKDFGTHSADSIDYPDVAIPVAEAVAAGEFDRGILICGTGIGIGIAANKVKG
IRAALVHDSFSAKATRQHNDSNIMTMGERVIGPGLALDLVTTWLDTDFEGGRHSNRVDKMSAYESK
>Mature_146_residues
MKIAIGADHGGFNLKKEIVALLEELGHEYKDFGTHSADSIDYPDVAIPVAEAVAAGEFDRGILICGTGIGIGIAANKVKG
IRAALVHDSFSAKATRQHNDSNIMTMGERVIGPGLALDLVTTWLDTDFEGGRHSNRVDKMSAYESK

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=145, Percent_Identity=54.4827586206897, Blast_Score=154, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15582; Mature: 15582

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIGADHGGFNLKKEIVALLEELGHEYKDFGTHSADSIDYPDVAIPVAEAVAAGEFDR
CEEEEECCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
GILICGTGIGIGIAANKVKGIRAALVHDSFSAKATRQHNDSNIMTMGERVIGPGLALDLV
CEEEEECCCCCCEECHHHCHHHHHHHHCCCCCCHHCCCCCCCEEEECHHHCCCCHHHHHH
TTWLDTDFEGGRHSNRVDKMSAYESK
HHHHCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKIAIGADHGGFNLKKEIVALLEELGHEYKDFGTHSADSIDYPDVAIPVAEAVAAGEFDR
CEEEEECCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
GILICGTGIGIGIAANKVKGIRAALVHDSFSAKATRQHNDSNIMTMGERVIGPGLALDLV
CEEEEECCCCCCEECHHHCHHHHHHHHCCCCCCHHCCCCCCCEEEECHHHCCCCHHHHHH
TTWLDTDFEGGRHSNRVDKMSAYESK
HHHHCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]