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The map label for this gene is cscA [H]

Identifier: 229917000

GI number: 229917000

Start: 1250657

End: 1251943

Strand: Direct

Name: cscA [H]

Synonym: EAT1b_1274

Alternate gene names: 229917000

Gene position: 1250657-1251943 (Clockwise)

Preceding gene: 229916999

Following gene: 229917001

Centisome position: 41.69

GC content: 41.96

Gene sequence:

>1287_bases
ATGACCTATCGTCCTATCTACCATTTCGCACCAGCTTTTGGATGGATGAACGATCCTAATGGACTTTGTCAGTTCAACGG
TGTCTACCATCTGTTTTATCAGTATCATCCGTTTGGCTTAAATTGGGATGATATGCATTGGGGGCATGCCACCTCAACAG
ATTTAATCCGTTGGGAACATAAGCCGATTGCTCTACGTCCAGGAGATGATTACGATCGAGACGGTATTTTTTCAGGTAGT
GCGTTAATCAAAGACGACATGATTCATTTGTATTACACAGGCAACACGTGGTTAGATGAAAAGCGTGAAACGTTACGTCA
AGTTCAATGTCTTGCCACATCAAAAGATGGACTATCGTTTCAAAAAAGTGTACACAATCCGCTCATATCAGAAATTCCCG
AACATGGAAATGCCCATGTTCGGGATCCCAAAGTTTGGGCTGAAAACGATAAATTCTATATGATTCTCGGAACACGTAAT
GAACTGAATGGAAAAGTAGTGCTCTACGAATCAGATGACGCCCTTCACTGGAGGGAACAAGGTATCATTGCAGAGGGAAC
TGGGGAGCATGGATGGATGTGGGAATGTCCCGACCTTTTTACTCTAAATGGTCATGATGTATTGTTACTCTCTCCACAAG
GAATGGAACCAATAGGAGATCGCTATCACAACCTACATCAATGCGGTTATTTTATAGGTGAGTTCGATATAACTACGACA
CAGTTCACACATGATTCGTTCTCCGAGTTAGATTATGGTCATGACTTTTACGCTGCCCAAACCTTTCTAGATGATCAAGG
TAGACGAATTTTGATTGCTTGGAACGCGATGTGGGAACAAGATTGGCCGGAGCAACGAGAAGGCTGGACGAACCAGATGA
CCATACCACGTGAATTAAACATAAGGAATAATCGGCTCTGGATGACTCCGATTGAAGAAATGAAATGGTTACGTCAATGC
TCCTTCCCTCTCAAATCACCTTTTTTCTCAGGAAAAGCGTTTGAGCTTGAGTTTGACGTGGAAGGAGAATTTCAGTTCAG
TTGTTTTCAAGGTACTGCTACCCTTTCCGTCGCTAAAGGCTATATCCAACTTGTTCGAAACGAAACAGAACGAAGGATTC
AGGCTAACTGTGAACGAGTTCGTTTATTCATCGATTCTTCTTCTATCGAAGTTTTCGTAAATGATGGAGAGTATGTCTTT
TCTAGTCGCATCTATCCAACCAATCACACCTTACAACATCACCTCACTGGAAACATTTCAAATATTATGTACCATTCCTT
TGAGTAA

Upstream 100 bases:

>100_bases
TACTTACGCGTCGTGCGCCTTCAATTGAGCCACTCACTGTCGAAATTTATGGAGAAAAGCAGACTATTACGGAAACATTT
ACAAGTGAAGTGTTATCAGT

Downstream 100 bases:

>100_bases
GTATATTGAAGCAGGTCCGAAATCAGTCTGGATCTGCTTTTTTGCATATTATGAAGCATTTTGTAATTGACATTATTTTG
AAGACTGCTACCGTTAATGT

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTYRPIYHFAPAFGWMNDPNGLCQFNGVYHLFYQYHPFGLNWDDMHWGHATSTDLIRWEHKPIALRPGDDYDRDGIFSGS
ALIKDDMIHLYYTGNTWLDEKRETLRQVQCLATSKDGLSFQKSVHNPLISEIPEHGNAHVRDPKVWAENDKFYMILGTRN
ELNGKVVLYESDDALHWREQGIIAEGTGEHGWMWECPDLFTLNGHDVLLLSPQGMEPIGDRYHNLHQCGYFIGEFDITTT
QFTHDSFSELDYGHDFYAAQTFLDDQGRRILIAWNAMWEQDWPEQREGWTNQMTIPRELNIRNNRLWMTPIEEMKWLRQC
SFPLKSPFFSGKAFELEFDVEGEFQFSCFQGTATLSVAKGYIQLVRNETERRIQANCERVRLFIDSSSIEVFVNDGEYVF
SSRIYPTNHTLQHHLTGNISNIMYHSFE

Sequences:

>Translated_428_residues
MTYRPIYHFAPAFGWMNDPNGLCQFNGVYHLFYQYHPFGLNWDDMHWGHATSTDLIRWEHKPIALRPGDDYDRDGIFSGS
ALIKDDMIHLYYTGNTWLDEKRETLRQVQCLATSKDGLSFQKSVHNPLISEIPEHGNAHVRDPKVWAENDKFYMILGTRN
ELNGKVVLYESDDALHWREQGIIAEGTGEHGWMWECPDLFTLNGHDVLLLSPQGMEPIGDRYHNLHQCGYFIGEFDITTT
QFTHDSFSELDYGHDFYAAQTFLDDQGRRILIAWNAMWEQDWPEQREGWTNQMTIPRELNIRNNRLWMTPIEEMKWLRQC
SFPLKSPFFSGKAFELEFDVEGEFQFSCFQGTATLSVAKGYIQLVRNETERRIQANCERVRLFIDSSSIEVFVNDGEYVF
SSRIYPTNHTLQHHLTGNISNIMYHSFE
>Mature_427_residues
TYRPIYHFAPAFGWMNDPNGLCQFNGVYHLFYQYHPFGLNWDDMHWGHATSTDLIRWEHKPIALRPGDDYDRDGIFSGSA
LIKDDMIHLYYTGNTWLDEKRETLRQVQCLATSKDGLSFQKSVHNPLISEIPEHGNAHVRDPKVWAENDKFYMILGTRNE
LNGKVVLYESDDALHWREQGIIAEGTGEHGWMWECPDLFTLNGHDVLLLSPQGMEPIGDRYHNLHQCGYFIGEFDITTTQ
FTHDSFSELDYGHDFYAAQTFLDDQGRRILIAWNAMWEQDWPEQREGWTNQMTIPRELNIRNNRLWMTPIEEMKWLRQCS
FPLKSPFFSGKAFELEFDVEGEFQFSCFQGTATLSVAKGYIQLVRNETERRIQANCERVRLFIDSSSIEVFVNDGEYVFS
SRIYPTNHTLQHHLTGNISNIMYHSFE

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=323, Percent_Identity=29.1021671826625, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 50172; Mature: 50040

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYRPIYHFAPAFGWMNDPNGLCQFNGVYHLFYQYHPFGLNWDDMHWGHATSTDLIRWEH
CCCCCCHHHCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECC
KPIALRPGDDYDRDGIFSGSALIKDDMIHLYYTGNTWLDEKRETLRQVQCLATSKDGLSF
CCEEECCCCCCCCCCEECCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHH
QKSVHNPLISEIPEHGNAHVRDPKVWAENDKFYMILGTRNELNGKVVLYESDDALHWREQ
HHHHCCCHHHHCCCCCCCEECCCCEEECCCCEEEEEECCCCCCCEEEEEECCCCEEHHHC
GIIAEGTGEHGWMWECPDLFTLNGHDVLLLSPQGMEPIGDRYHNLHQCGYFIGEFDITTT
CEEEECCCCCCCEEECCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEEEEE
QFTHDSFSELDYGHDFYAAQTFLDDQGRRILIAWNAMWEQDWPEQREGWTNQMTIPRELN
EECCCCHHHCCCCCCHHHHHHEECCCCCEEEEEEECCCCCCCCHHHCCCCCCEECCEECC
IRNNRLWMTPIEEMKWLRQCSFPLKSPFFSGKAFELEFDVEGEFQFSCFQGTATLSVAKG
CCCCEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCEEEHHHHH
YIQLVRNETERRIQANCERVRLFIDSSSIEVFVNDGEYVFSSRIYPTNHTLQHHLTGNIS
HHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCEEEEEEEECCCCCEEEEECCCCC
NIMYHSFE
CEEEECCC
>Mature Secondary Structure 
TYRPIYHFAPAFGWMNDPNGLCQFNGVYHLFYQYHPFGLNWDDMHWGHATSTDLIRWEH
CCCCCHHHCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEECC
KPIALRPGDDYDRDGIFSGSALIKDDMIHLYYTGNTWLDEKRETLRQVQCLATSKDGLSF
CCEEECCCCCCCCCCEECCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHH
QKSVHNPLISEIPEHGNAHVRDPKVWAENDKFYMILGTRNELNGKVVLYESDDALHWREQ
HHHHCCCHHHHCCCCCCCEECCCCEEECCCCEEEEEECCCCCCCEEEEEECCCCEEHHHC
GIIAEGTGEHGWMWECPDLFTLNGHDVLLLSPQGMEPIGDRYHNLHQCGYFIGEFDITTT
CEEEECCCCCCCEEECCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEEEEE
QFTHDSFSELDYGHDFYAAQTFLDDQGRRILIAWNAMWEQDWPEQREGWTNQMTIPRELN
EECCCCHHHCCCCCCHHHHHHEECCCCCEEEEEEECCCCCCCCHHHCCCCCCEECCEECC
IRNNRLWMTPIEEMKWLRQCSFPLKSPFFSGKAFELEFDVEGEFQFSCFQGTATLSVAKG
CCCCEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCEEEHHHHH
YIQLVRNETERRIQANCERVRLFIDSSSIEVFVNDGEYVFSSRIYPTNHTLQHHLTGNIS
HHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCEEEEEEEECCCCCEEEEECCCCC
NIMYHSFE
CEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218016 [H]