Click here to switch to the map view.

The map label for this gene is hadL [H]

Identifier: 229916963

GI number: 229916963

Start: 1213835

End: 1214500

Strand: Direct

Name: hadL [H]

Synonym: EAT1b_1237

Alternate gene names: 229916963

Gene position: 1213835-1214500 (Clockwise)

Preceding gene: 229916956

Following gene: 229916966

Centisome position: 40.46

GC content: 51.35

Gene sequence:

>666_bases
ATGAAACAACCTATCAAAGCCATCGTCTTTGACGTATATGGCACATTATTCGACGTCCATTCGGTGAAGGATGCCTGTGA
ACAAGAGTTCCCTGAAAAAGGAGCTGCCATCAGCCAAGTATGGCGGACGAAACAACTCGAGTATTTCTTTTGGCGCCACA
TGATGGGACGTTATGAAGATTTCGATAAAGTGACCCGGGACGCACTCCGTTATGCGCTCGCCGTGCATGATGCAACATCG
ACAGAGGAAATCGAACAACGTCTGGTCGCATCGTATGCGCACCTCACCCCGTATGAAGAGGTCGAGTCGGTCCTAAAAGA
GTTGCATGACGTGAAGACCGTCATCCTCTCGAACGGGACGGACGAGATGCTTGAGGCACTGCTTCGCAACGCCGGTCTTA
GCGATTATTTCGATGAGGTCGTCAGCGTCGACGGCATCAAACAACCGAAACCGACGCCTGCTGCCTACATGCACTGGTTC
AAGACGTCCGGTCTTCGTCGTGACGAGGTGCTCTTCTTATCGTCGAACGGATGGGACATCGCCGGGGCGAAAAACTTTGG
CTTTCATGCCGCCTGGATCAATCGGAACGACTTACCGTTCGAGGAGTTCGATGTTAAACCAGATGCCGTCTATCCAAACT
TGAATCATTTGTCAGAATGGCTCTAG

Upstream 100 bases:

>100_bases
TTTGACGGAATATTGATTTGGAATATACATGCTGTACCCCCTTTCTCCTATTATATTCTGATCAAGTCGGGTATAACGAC
ACAGAAGGAGGAATGTTTTT

Downstream 100 bases:

>100_bases
TCCGTGAACGACCTCGTGAAAATGAGGTCGTTTTGTGCATCTTCCCCCATGAAAAAGGTATGCACCCCCGTCTGTTCGAA
CCCGTTCCGTCGATAGAATC

Product: haloacid dehalogenase, type II

Products: NA

Alternate protein names: 2-haloalkanoic acid dehalogenase; Halocarboxylic acid halidohydrolase; L-2-haloacid dehalogenase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKQPIKAIVFDVYGTLFDVHSVKDACEQEFPEKGAAISQVWRTKQLEYFFWRHMMGRYEDFDKVTRDALRYALAVHDATS
TEEIEQRLVASYAHLTPYEEVESVLKELHDVKTVILSNGTDEMLEALLRNAGLSDYFDEVVSVDGIKQPKPTPAAYMHWF
KTSGLRRDEVLFLSSNGWDIAGAKNFGFHAAWINRNDLPFEEFDVKPDAVYPNLNHLSEWL

Sequences:

>Translated_221_residues
MKQPIKAIVFDVYGTLFDVHSVKDACEQEFPEKGAAISQVWRTKQLEYFFWRHMMGRYEDFDKVTRDALRYALAVHDATS
TEEIEQRLVASYAHLTPYEEVESVLKELHDVKTVILSNGTDEMLEALLRNAGLSDYFDEVVSVDGIKQPKPTPAAYMHWF
KTSGLRRDEVLFLSSNGWDIAGAKNFGFHAAWINRNDLPFEEFDVKPDAVYPNLNHLSEWL
>Mature_221_residues
MKQPIKAIVFDVYGTLFDVHSVKDACEQEFPEKGAAISQVWRTKQLEYFFWRHMMGRYEDFDKVTRDALRYALAVHDATS
TEEIEQRLVASYAHLTPYEEVESVLKELHDVKTVILSNGTDEMLEALLRNAGLSDYFDEVVSVDGIKQPKPTPAAYMHWF
KTSGLRRDEVLFLSSNGWDIAGAKNFGFHAAWINRNDLPFEEFDVKPDAVYPNLNHLSEWL

Specific function: Catalyzes the hydrolytic dehalogenation of small (S)-2- haloalkanoic acids to yield the corresponding (R)-2- hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2 [H]

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. S-2- haloalkanoic acid dehalogenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006388
- InterPro:   IPR006328
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.8.1.2 [H]

Molecular weight: Translated: 25497; Mature: 25497

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPIKAIVFDVYGTLFDVHSVKDACEQEFPEKGAAISQVWRTKQLEYFFWRHMMGRYED
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FDKVTRDALRYALAVHDATSTEEIEQRLVASYAHLTPYEEVESVLKELHDVKTVILSNGT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
DEMLEALLRNAGLSDYFDEVVSVDGIKQPKPTPAAYMHWFKTSGLRRDEVLFLSSNGWDI
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEE
AGAKNFGFHAAWINRNDLPFEEFDVKPDAVYPNLNHLSEWL
CCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKQPIKAIVFDVYGTLFDVHSVKDACEQEFPEKGAAISQVWRTKQLEYFFWRHMMGRYED
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FDKVTRDALRYALAVHDATSTEEIEQRLVASYAHLTPYEEVESVLKELHDVKTVILSNGT
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
DEMLEALLRNAGLSDYFDEVVSVDGIKQPKPTPAAYMHWFKTSGLRRDEVLFLSSNGWDI
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEE
AGAKNFGFHAAWINRNDLPFEEFDVKPDAVYPNLNHLSEWL
CCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1588303; 1556080 [H]