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The map label for this gene is lacE [H]

Identifier: 229916778

GI number: 229916778

Start: 999123

End: 1000793

Strand: Reverse

Name: lacE [H]

Synonym: EAT1b_1051

Alternate gene names: 229916778

Gene position: 1000793-999123 (Counterclockwise)

Preceding gene: 229916779

Following gene: 229916777

Centisome position: 33.36

GC content: 49.67

Gene sequence:

>1671_bases
ATGAACGGTTTAATCGCAAGAATTGAAAAGATGAAACCAGCATTCGAAAAGATTTCGAACAACCCGTACTTACGAGCGAT
TCGAGATGGATTCATTAGTTTGATCCCAGTCATTTTATTCTCAAGTATTTTCCTATTAATTGCATTCGTCCCTAACATTT
TTGGTGTGTACTTAAGCGAGGAATGGGTCAACCGACTCATGAAAGCATACGGATACTCGATGGGGCTAGTCGGTCTGCTT
ATGTCAGCGACCATCGCAAAATCGTTGACGGAGACATTCAACGGGCAACTTCCGAAGACGAGACAAATCAACTCGGTCTC
GACGATGGTCGTATCGATCATCGGGTTCGCGTTAATCGGGGTCGATCAGATCGAAGGTGGCCTATCAAACGGATTCATGG
GTACGAAAGGTCTCTTGACGGCATTCGTGGTCGCCTTCATCGTTCCGAATATTTATAAGTTCTGTGTGAAACGCAACATT
ACCATCAAAATGCCGAAAGAAGTACCAGGAAACATCGCTCAAACCTTTGCGGACTTGATTCCGCTCGCGACATCCATCAT
CTTCTTCTGGGTGTTTGATATGGTATTCCGTAGTTTCGCCGCAGAAGGATTCAGTGCTTGGATCATCGACGTGTTCCAAC
CACTCTTCTCAGCTGCAGACGGTTATCCGGGACTCGCATTGATCTTCGGTGCGATGGCATTCTTCTGGTTCGTCGGAATT
CATGGACCATCGATTGTAGAACCAGCCGTATCGGCGATCTATATCACAAATGTTGAGCAAAACTTACAGTTGTTCCAAGC
GGGCGAACAGGCGACGAATGTCCTCTCGCAAGGGTCGCAGTACTTCATCGCGACATTAGGTGGTACTGGGGCGACACTCG
TCATCACGTTCATGATGGCGTTCATGGCGAAGTCGAAACAGTTGAAGTCGGTTGGACGCGCTTCTTCTGTACCGGTTCTG
TTCGGGATTAACGAACCGATCTTGTTCGGGGCGCCAATCGTCTTGAACCCGGTCTTCTTCATCCCGTTCATCGCGGCACC
AATCGTCAACGTCTGGTTATTGAAGGCGTTCATTGACATCGGTGGGATGAACGGATTCATCTACAACTTACCTTGGACGA
CTCCAGGGCCAATCGGTTTGATTCTCGGTACAGGATTCGCGCCACTCGCCTTCATCCTCGCACCGACGTTGATCATCATC
GACTTGTTGATTTACTATCCGTTCTTTAAAGTGTATGACAAGCAGTTGGTCGAGCAAGAGCGCTTGGCTGTCCTTGAAGA
AGAACAAACGCCGGATACGGGTGCGGAAATCGCGACAAGCGCGACGGAGGCACGTGTCGTGAAGCCGGTGGATGCATCGA
AAACGTATAACGTCCTCGTCTTGTGTGCGAACGGTGCCACGAGCGGGATGCTCGCGAATGCGATTGCCAAAGGGGCGAAG
GAAGAAGGGCTTCAGCTCGAGTCGACTGCTATGGCTTACGGTCAACATAAAGCGGTCATTTCAGAGTTTGACTTGATCAT
CCTTGCTCCGCAGATGGCATCGATGCTTGGGGAGTTGGAACAAGACTCGGCATCTAGTGATACGAACGCCGTTTCGACAT
CGGGTGCAGAATATGTGGCGCTTTGTCGCGATCCTAAAGGGGCACTCGCCTTCGCGATGAAGCATATGTAA

Upstream 100 bases:

>100_bases
CATGATGCAACATATGATCGAGCTCTATCGCCGGTCAAATTAACTTAGACGAACGGGTCGCACGACCCTTCGATCATTCA
CTAGTAAAGGAGGAAATGGC

Downstream 100 bases:

>100_bases
TACAAAAGGGGCTGGTCATTCACAGCCCCTCTAATCTTTTCAGTCTAGACAAAGAGGTGGAATCGAAATGAGACAATTTC
CGGAAGAGTTTATTTTTGGT

Product: PTS system, lactose/cellobiose family IIC subunit

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MNGLIARIEKMKPAFEKISNNPYLRAIRDGFISLIPVILFSSIFLLIAFVPNIFGVYLSEEWVNRLMKAYGYSMGLVGLL
MSATIAKSLTETFNGQLPKTRQINSVSTMVVSIIGFALIGVDQIEGGLSNGFMGTKGLLTAFVVAFIVPNIYKFCVKRNI
TIKMPKEVPGNIAQTFADLIPLATSIIFFWVFDMVFRSFAAEGFSAWIIDVFQPLFSAADGYPGLALIFGAMAFFWFVGI
HGPSIVEPAVSAIYITNVEQNLQLFQAGEQATNVLSQGSQYFIATLGGTGATLVITFMMAFMAKSKQLKSVGRASSVPVL
FGINEPILFGAPIVLNPVFFIPFIAAPIVNVWLLKAFIDIGGMNGFIYNLPWTTPGPIGLILGTGFAPLAFILAPTLIII
DLLIYYPFFKVYDKQLVEQERLAVLEEEQTPDTGAEIATSATEARVVKPVDASKTYNVLVLCANGATSGMLANAIAKGAK
EEGLQLESTAMAYGQHKAVISEFDLIILAPQMASMLGELEQDSASSDTNAVSTSGAEYVALCRDPKGALAFAMKHM

Sequences:

>Translated_556_residues
MNGLIARIEKMKPAFEKISNNPYLRAIRDGFISLIPVILFSSIFLLIAFVPNIFGVYLSEEWVNRLMKAYGYSMGLVGLL
MSATIAKSLTETFNGQLPKTRQINSVSTMVVSIIGFALIGVDQIEGGLSNGFMGTKGLLTAFVVAFIVPNIYKFCVKRNI
TIKMPKEVPGNIAQTFADLIPLATSIIFFWVFDMVFRSFAAEGFSAWIIDVFQPLFSAADGYPGLALIFGAMAFFWFVGI
HGPSIVEPAVSAIYITNVEQNLQLFQAGEQATNVLSQGSQYFIATLGGTGATLVITFMMAFMAKSKQLKSVGRASSVPVL
FGINEPILFGAPIVLNPVFFIPFIAAPIVNVWLLKAFIDIGGMNGFIYNLPWTTPGPIGLILGTGFAPLAFILAPTLIII
DLLIYYPFFKVYDKQLVEQERLAVLEEEQTPDTGAEIATSATEARVVKPVDASKTYNVLVLCANGATSGMLANAIAKGAK
EEGLQLESTAMAYGQHKAVISEFDLIILAPQMASMLGELEQDSASSDTNAVSTSGAEYVALCRDPKGALAFAMKHM
>Mature_556_residues
MNGLIARIEKMKPAFEKISNNPYLRAIRDGFISLIPVILFSSIFLLIAFVPNIFGVYLSEEWVNRLMKAYGYSMGLVGLL
MSATIAKSLTETFNGQLPKTRQINSVSTMVVSIIGFALIGVDQIEGGLSNGFMGTKGLLTAFVVAFIVPNIYKFCVKRNI
TIKMPKEVPGNIAQTFADLIPLATSIIFFWVFDMVFRSFAAEGFSAWIIDVFQPLFSAADGYPGLALIFGAMAFFWFVGI
HGPSIVEPAVSAIYITNVEQNLQLFQAGEQATNVLSQGSQYFIATLGGTGATLVITFMMAFMAKSKQLKSVGRASSVPVL
FGINEPILFGAPIVLNPVFFIPFIAAPIVNVWLLKAFIDIGGMNGFIYNLPWTTPGPIGLILGTGFAPLAFILAPTLIII
DLLIYYPFFKVYDKQLVEQERLAVLEEEQTPDTGAEIATSATEARVVKPVDASKTYNVLVLCANGATSGMLANAIAKGAK
EEGLQLESTAMAYGQHKAVISEFDLIILAPQMASMLGELEQDSASSDTNAVSTSGAEYVALCRDPKGALAFAMKHM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=469, Percent_Identity=26.0127931769723, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60144; Mature: 60144

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCHHHHHHHHCC
>Mature Secondary Structure
MNGLIARIEKMKPAFEKISNNPYLRAIRDGFISLIPVILFSSIFLLIAFVPNIFGVYLSE
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EWVNRLMKAYGYSMGLVGLLMSATIAKSLTETFNGQLPKTRQINSVSTMVVSIIGFALIG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VDQIEGGLSNGFMGTKGLLTAFVVAFIVPNIYKFCVKRNITIKMPKEVPGNIAQTFADLI
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
PLATSIIFFWVFDMVFRSFAAEGFSAWIIDVFQPLFSAADGYPGLALIFGAMAFFWFVGI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
HGPSIVEPAVSAIYITNVEQNLQLFQAGEQATNVLSQGSQYFIATLGGTGATLVITFMMA
CCCHHHHHHHHEEEEECCHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
FMAKSKQLKSVGRASSVPVLFGINEPILFGAPIVLNPVFFIPFIAAPIVNVWLLKAFIDI
HHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGMNGFIYNLPWTTPGPIGLILGTGFAPLAFILAPTLIIIDLLIYYPFFKVYDKQLVEQE
CCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAVLEEEQTPDTGAEIATSATEARVVKPVDASKTYNVLVLCANGATSGMLANAIAKGAK
HHHHHHCCCCCCCCHHHHHCHHHHHEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHH
EEGLQLESTAMAYGQHKAVISEFDLIILAPQMASMLGELEQDSASSDTNAVSTSGAEYVA
HCCCEEHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LCRDPKGALAFAMKHM
EECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA