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The map label for this gene is lacF [H]

Identifier: 229916779

GI number: 229916779

Start: 1000851

End: 1001165

Strand: Reverse

Name: lacF [H]

Synonym: EAT1b_1052

Alternate gene names: 229916779

Gene position: 1001165-1000851 (Counterclockwise)

Preceding gene: 229916781

Following gene: 229916778

Centisome position: 33.37

GC content: 51.43

Gene sequence:

>315_bases
ATGACGAAAGAAGAGTCAGCATTGGTAGGTTTGGAAATTGTCGCTCACGCAGGAGATGCGCGTTCGAATCTGATGCGTGC
GCTCGAGGCAGCGAAAGAAGGGGATTACGACCGAGCTGAATCGCTAGTTAAACAAGCAGATGAATCAATCTCGCTCGCAC
ATGCGACACAGACGAGCATGATTCAAGCGGAAGCACGAGGCGAAGGTGCGGAATTCGGGTTCATCATGGTGCATGGGCAG
GACCACTTGATGACGACGATGCTCTTGCGCGACATGATGCAACATATGATCGAGCTCTATCGCCGGTCAAATTAA

Upstream 100 bases:

>100_bases
TTAATTTGATTGAAAACGTTTACAAGAGTTCTTTCTGGTGCTACTATATGAATGTGGATATGACATATCGAAAAAAAACA
AACACAAAGGGGAGTTTAAC

Downstream 100 bases:

>100_bases
CTTAGACGAACGGGTCGCACGACCCTTCGATCATTCACTAGTAAAGGAGGAAATGGCATGAACGGTTTAATCGCAAGAAT
TGAAAAGATGAAACCAGCAT

Product: phosphotransferase system PTS lactose/cellobiose-specific IIA subunit

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 104; Mature: 103

Protein sequence:

>104_residues
MTKEESALVGLEIVAHAGDARSNLMRALEAAKEGDYDRAESLVKQADESISLAHATQTSMIQAEARGEGAEFGFIMVHGQ
DHLMTTMLLRDMMQHMIELYRRSN

Sequences:

>Translated_104_residues
MTKEESALVGLEIVAHAGDARSNLMRALEAAKEGDYDRAESLVKQADESISLAHATQTSMIQAEARGEGAEFGFIMVHGQ
DHLMTTMLLRDMMQHMIELYRRSN
>Mature_103_residues
TKEESALVGLEIVAHAGDARSNLMRALEAAKEGDYDRAESLVKQADESISLAHATQTSMIQAEARGEGAEFGFIMVHGQD
HLMTTMLLRDMMQHMIELYRRSN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=99, Percent_Identity=38.3838383838384, Blast_Score=69, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 11566; Mature: 11435

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
8.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
7.8 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEESALVGLEIVAHAGDARSNLMRALEAAKEGDYDRAESLVKQADESISLAHATQTSM
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQAEARGEGAEFGFIMVHGQDHLMTTMLLRDMMQHMIELYRRSN
HHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEESALVGLEIVAHAGDARSNLMRALEAAKEGDYDRAESLVKQADESISLAHATQTSM
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IQAEARGEGAEFGFIMVHGQDHLMTTMLLRDMMQHMIELYRRSN
HHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400164; 12397186 [H]