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The map label for this gene is gatB [H]

Identifier: 229916719

GI number: 229916719

Start: 933973

End: 935403

Strand: Reverse

Name: gatB [H]

Synonym: EAT1b_0992

Alternate gene names: 229916719

Gene position: 935403-933973 (Counterclockwise)

Preceding gene: 229916720

Following gene: 229916718

Centisome position: 31.18

GC content: 54.02

Gene sequence:

>1431_bases
ATGAACTTTGAAACGATCATCGGGATCGAGGTGCACGCCGAGCTGAGCACGAAGTCGAAGATTTGGTGTGGCTGCTCACG
CACTTACGGTGCCGAAACGAATACACAGACGTGTCCGATTTGTCTCGGACACCCGGGCGTCTTGCCTAAATTGAACGAAG
AAGCGGTCAACCTCGCCATCAAAGCGGCAATGGCACTCAACTGTGAGATTGCGACAGATACGAAGTTCGACCGCAAGAAC
TACTTCTACCCAGACACGCCGAAAGCGTATCAAATCTCGCAGTTTGATAAGCCAATCGGTTCAAACGGTCACGTCGACGT
CGAAGTGGACGGAGAGATGAAACGATTCCGCATCGAGCGCGTGCATTTAGAAGAAGATGCCGGGAAGATGAACCACACGG
GCGAGAAACACTCGGTCGTCGACTTCAACCGGACCGGTTCACCACTCATCGAGATTGTCAGTGAAGCAGACATGCGCTCA
CCGAAAGATGCGGTCGCGTATCTCGAGCGCTTGAAAGAAGTCCTCTCGTTCGCTGGCGTCTCGGACGTCAAGATGGAAGA
AGGGTCGCTTCGCTGTGACTGTAACATCTCGGTCCGTCCATTCGGGCAGGAGAAGTTCGGTACGAAGACAGAGCTCAAGA
ACTTGAACTCGTTCTCAAACGTCTTAAAGGCACTCGACTATGAAGAGACGCGTCATCGCAAATTGCTTCTCTCTGGTGGC
GTCATGCGTCAAGAGACGCTCCGTTACGACGAAGCGGCGAAAAAGACGATTCTCATGCGTGTCAAAGAAGGGGCAGCGGA
CTACCGTTACTTCCCAGAACCGGACCTCGTCCGTCTCGAAATCGATGAGGCATGGAAAGAGCGCATCCGCGCGACGATTC
CAGAGATGCCGGCAGCTCGTCGTGAACGCTACATGAGCCAATACGGTCTCTCGGCATACGACGCAAAAGCCCTCACGTCA
ACGCGTGAGTTCTCGGACTTCTTCGAAGCGACGACAGCGGCCGGTGCGGAGCCGAAAGCGGCAGCGAACTGGCTCATGGG
TGAAGTGCTCGGTCACTTGAACAAGTCGGATGAAACGGTCGAGACGATGAAGTTGACACCGGAATCACTCGCTGAGCTCA
TCCGCTTGATTGCGGAAGGGACGATTTCATCGAAAATCGCGAAGCGTGTCTTCACGGCGATGATGGAAGAAGGCGCTGAG
CCGAAAGCGTATGTGGAAGCGAACGGACTCGCCCAAATCTCGGACGAGAACTTACTCCGTGGCTACATCCTCGAATTGTT
CGAGGCGCATCCGCAAATGATCGAAGACTTCAAGAACGGGAAAGACCGTGCGAAAGGCTTCATCATCGGCCAAATCATGA
AACAGACGAAAGGGATGGCGAACCCGGCACTCCTCGATGGCTTGTTCCACGAAGAGATCGCAAAACGCTAA

Upstream 100 bases:

>100_bases
CGGGAACTACTTTGATGAATCAACACTTTACCGCGTGGCCCATGCTTTTGAACAAGCAAACGGCGGTTTCAAGATGCCAC
AGCTCTAAGGAGGACGTACG

Downstream 100 bases:

>100_bases
GCAGGCGGCGAGACACTTGTGTCTCGCTTTTCGCTCGAGTTGAGCATAGGGGAGAGAATCAATATGCAACGGAAACGTGC
GCGGGTCATTTATAACCCGA

Product: glutamyl-tRNA(Gln) amidotransferase, B subunit

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETIIGIEVHAELSTKSKIWCGCSRTYGAETNTQTCPICLGHPGVLPKLNEEAVNLAIKAAMALNCEIATDTKFDRKN
YFYPDTPKAYQISQFDKPIGSNGHVDVEVDGEMKRFRIERVHLEEDAGKMNHTGEKHSVVDFNRTGSPLIEIVSEADMRS
PKDAVAYLERLKEVLSFAGVSDVKMEEGSLRCDCNISVRPFGQEKFGTKTELKNLNSFSNVLKALDYEETRHRKLLLSGG
VMRQETLRYDEAAKKTILMRVKEGAADYRYFPEPDLVRLEIDEAWKERIRATIPEMPAARRERYMSQYGLSAYDAKALTS
TREFSDFFEATTAAGAEPKAAANWLMGEVLGHLNKSDETVETMKLTPESLAELIRLIAEGTISSKIAKRVFTAMMEEGAE
PKAYVEANGLAQISDENLLRGYILELFEAHPQMIEDFKNGKDRAKGFIIGQIMKQTKGMANPALLDGLFHEEIAKR

Sequences:

>Translated_476_residues
MNFETIIGIEVHAELSTKSKIWCGCSRTYGAETNTQTCPICLGHPGVLPKLNEEAVNLAIKAAMALNCEIATDTKFDRKN
YFYPDTPKAYQISQFDKPIGSNGHVDVEVDGEMKRFRIERVHLEEDAGKMNHTGEKHSVVDFNRTGSPLIEIVSEADMRS
PKDAVAYLERLKEVLSFAGVSDVKMEEGSLRCDCNISVRPFGQEKFGTKTELKNLNSFSNVLKALDYEETRHRKLLLSGG
VMRQETLRYDEAAKKTILMRVKEGAADYRYFPEPDLVRLEIDEAWKERIRATIPEMPAARRERYMSQYGLSAYDAKALTS
TREFSDFFEATTAAGAEPKAAANWLMGEVLGHLNKSDETVETMKLTPESLAELIRLIAEGTISSKIAKRVFTAMMEEGAE
PKAYVEANGLAQISDENLLRGYILELFEAHPQMIEDFKNGKDRAKGFIIGQIMKQTKGMANPALLDGLFHEEIAKR
>Mature_476_residues
MNFETIIGIEVHAELSTKSKIWCGCSRTYGAETNTQTCPICLGHPGVLPKLNEEAVNLAIKAAMALNCEIATDTKFDRKN
YFYPDTPKAYQISQFDKPIGSNGHVDVEVDGEMKRFRIERVHLEEDAGKMNHTGEKHSVVDFNRTGSPLIEIVSEADMRS
PKDAVAYLERLKEVLSFAGVSDVKMEEGSLRCDCNISVRPFGQEKFGTKTELKNLNSFSNVLKALDYEETRHRKLLLSGG
VMRQETLRYDEAAKKTILMRVKEGAADYRYFPEPDLVRLEIDEAWKERIRATIPEMPAARRERYMSQYGLSAYDAKALTS
TREFSDFFEATTAAGAEPKAAANWLMGEVLGHLNKSDETVETMKLTPESLAELIRLIAEGTISSKIAKRVFTAMMEEGAE
PKAYVEANGLAQISDENLLRGYILELFEAHPQMIEDFKNGKDRAKGFIIGQIMKQTKGMANPALLDGLFHEEIAKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=34.7474747474747, Blast_Score=278, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI115534094, Length=294, Percent_Identity=34.3537414965986, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=27.9527559055118, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=29.6907216494845, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53434; Mature: 53434

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETIIGIEVHAELSTKSKIWCGCSRTYGAETNTQTCPICLGHPGVLPKLNEEAVNLAI
CCCCEEEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHH
KAAMALNCEIATDTKFDRKNYFYPDTPKAYQISQFDKPIGSNGHVDVEVDGEMKRFRIER
HHHHHEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCEEEEEECCCHHHHHHHH
VHLEEDAGKMNHTGEKHSVVDFNRTGSPLIEIVSEADMRSPKDAVAYLERLKEVLSFAGV
EEEHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSLRCDCNISVRPFGQEKFGTKTELKNLNSFSNVLKALDYEETRHRKLLLSGG
CCCEECCCCEEEEECEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHEEHHCCC
VMRQETLRYDEAAKKTILMRVKEGAADYRYFPEPDLVRLEIDEAWKERIRATIPEMPAAR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHH
RERYMSQYGLSAYDAKALTSTREFSDFFEATTAAGAEPKAAANWLMGEVLGHLNKSDETV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
ETMKLTPESLAELIRLIAEGTISSKIAKRVFTAMMEEGAEPKAYVEANGLAQISDENLLR
HHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCHHHH
GYILELFEAHPQMIEDFKNGKDRAKGFIIGQIMKQTKGMANPALLDGLFHEEIAKR
HHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETIIGIEVHAELSTKSKIWCGCSRTYGAETNTQTCPICLGHPGVLPKLNEEAVNLAI
CCCCEEEEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHH
KAAMALNCEIATDTKFDRKNYFYPDTPKAYQISQFDKPIGSNGHVDVEVDGEMKRFRIER
HHHHHEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCEEEEEECCCHHHHHHHH
VHLEEDAGKMNHTGEKHSVVDFNRTGSPLIEIVSEADMRSPKDAVAYLERLKEVLSFAGV
EEEHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSLRCDCNISVRPFGQEKFGTKTELKNLNSFSNVLKALDYEETRHRKLLLSGG
CCCEECCCCEEEEECEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHEEHHCCC
VMRQETLRYDEAAKKTILMRVKEGAADYRYFPEPDLVRLEIDEAWKERIRATIPEMPAAR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHH
RERYMSQYGLSAYDAKALTSTREFSDFFEATTAAGAEPKAAANWLMGEVLGHLNKSDETV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
ETMKLTPESLAELIRLIAEGTISSKIAKRVFTAMMEEGAEPKAYVEANGLAQISDENLLR
HHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEECCCHHHH
GYILELFEAHPQMIEDFKNGKDRAKGFIIGQIMKQTKGMANPALLDGLFHEEIAKR
HHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA