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The map label for this gene is gatA

Identifier: 229916720

GI number: 229916720

Start: 935416

End: 936858

Strand: Reverse

Name: gatA

Synonym: EAT1b_0993

Alternate gene names: 229916720

Gene position: 936858-935416 (Counterclockwise)

Preceding gene: 229916721

Following gene: 229916719

Centisome position: 31.23

GC content: 54.19

Gene sequence:

>1443_bases
ATGAGTTTATTTGACCACGGTGTAGCGAAACTGCATACGTTATTAAAAGAAGGCGAAGTCACGGTCACGGACCTCGTCAA
AGAGTCGTTCGACCGCATCGAAGCGACTGATGATCAAATCGGTGCGTTTTTGACGCTCAATGAGGATGCGTTCGAGCAAG
CGAAGCGCATGGATGAGCTCGCAAAGATGTCAGACAACCCGCTCTTTGGGATGCCGATCGGTGTGAAAGACAACATCGTC
ACAAAAGGGATGCGGACGACATGTGCCTCGACGTTCCTTGAGAACTTCGTCCCGGCACACGATGCGACGGTCGTCGAGCG
TCTTCATGACGCCGGTGCCCTCACAATCGGGAAGTTGAACATGGACGAGTTCGCGATGGGTTCATCAAACGAGAACTCAG
CGTTCAAGCCTGTCCGTAACCCATGGGACCCGACTCGTGTACCAGGAGGTTCTTCAGGCGGTTCAGCGGCATCGGTCGCA
GCTGGGCAAGTCCTCTTCAGCCTCGGTTCGGATACGGGTGGTTCGATTCGTCAACCAGCCGCATATTGTGGTGTCGTCGG
GATGAAGCCGACATACGGTCTCGTCTCACGTTTCGGTCTCGTCGCCTTCGCGTCGTCACTCGACCAAATCGGCCCGCTCA
CGCGCACGGTCGAAGACAATGCTTACTTATTGAATACGATTGCGGGTCATTGCGACATGGACTCGACGTCGGCTGAAGTC
GAGGTGCCGGACTATACGGCAGCGCTCAACCAGGATTTGACGGGCATGAAGTTCGCACTTCCAGAAGAATTCATGGCAGA
AGGTGTCGCGCCTGGTGTCCGTGAACAGATTGAAAAAGCGATCGAGACGCTCAAGTCTCTCGGTGCGACGATTGAGACGG
TTTCGATTCCGTCTGTGAAGTATGCCCTCTCGGCGTACTACTTGCTCGCCTCGTCAGAAGCCTCTTCAAACTTGGCTCGT
TTCGACGGTATCCGTTACGGACGTCGCGCCGAAGCGGACACGCTCGACGAAGTGTTCACATATTCGCGTGAACAAGGGTT
CGGGGACGAGGTCAAACGCCGGATCATGCTCGGGACATATGCCCTCAGCTCTGGTTACTACGATGCGTTCTACAAGAAAG
CCCAACAAGTCCGTACCCTCATGAAGCAGGACTTCGCGAAAGTGTTCGAAGAGTATGATGCCATCGTTGGACCAACTGCT
CCGACGACAGCGTTCAAACTCGGGGAACAGCTCGACGATCCGCTCACGATGTACGCGAACGATATCATGACGATTCCGGT
CAACTTGGTCGGTATCCCGGCTATCTCAGTTCCGGCAGGTCTTTCAGAAGGTCTCCCGGTCGGTCTTCAAATTATCGGGA
ACTACTTTGATGAATCAACACTTTACCGCGTGGCCCATGCTTTTGAACAAGCAAACGGCGGTTTCAAGATGCCACAGCTC
TAA

Upstream 100 bases:

>100_bases
TCCGTCCATCGCTTCCACGTGAGGAAGTCGAACGGATGGCACCTGACTGGGAGAACGGTCAAGTCCGTGTCCCTGCAGTC
TTTGAGTAAGGAGGCCTTTC

Downstream 100 bases:

>100_bases
GGAGGACGTACGATGAACTTTGAAACGATCATCGGGATCGAGGTGCACGCCGAGCTGAGCACGAAGTCGAAGATTTGGTG
TGGCTGCTCACGCACTTACG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSLFDHGVAKLHTLLKEGEVTVTDLVKESFDRIEATDDQIGAFLTLNEDAFEQAKRMDELAKMSDNPLFGMPIGVKDNIV
TKGMRTTCASTFLENFVPAHDATVVERLHDAGALTIGKLNMDEFAMGSSNENSAFKPVRNPWDPTRVPGGSSGGSAASVA
AGQVLFSLGSDTGGSIRQPAAYCGVVGMKPTYGLVSRFGLVAFASSLDQIGPLTRTVEDNAYLLNTIAGHCDMDSTSAEV
EVPDYTAALNQDLTGMKFALPEEFMAEGVAPGVREQIEKAIETLKSLGATIETVSIPSVKYALSAYYLLASSEASSNLAR
FDGIRYGRRAEADTLDEVFTYSREQGFGDEVKRRIMLGTYALSSGYYDAFYKKAQQVRTLMKQDFAKVFEEYDAIVGPTA
PTTAFKLGEQLDDPLTMYANDIMTIPVNLVGIPAISVPAGLSEGLPVGLQIIGNYFDESTLYRVAHAFEQANGGFKMPQL

Sequences:

>Translated_480_residues
MSLFDHGVAKLHTLLKEGEVTVTDLVKESFDRIEATDDQIGAFLTLNEDAFEQAKRMDELAKMSDNPLFGMPIGVKDNIV
TKGMRTTCASTFLENFVPAHDATVVERLHDAGALTIGKLNMDEFAMGSSNENSAFKPVRNPWDPTRVPGGSSGGSAASVA
AGQVLFSLGSDTGGSIRQPAAYCGVVGMKPTYGLVSRFGLVAFASSLDQIGPLTRTVEDNAYLLNTIAGHCDMDSTSAEV
EVPDYTAALNQDLTGMKFALPEEFMAEGVAPGVREQIEKAIETLKSLGATIETVSIPSVKYALSAYYLLASSEASSNLAR
FDGIRYGRRAEADTLDEVFTYSREQGFGDEVKRRIMLGTYALSSGYYDAFYKKAQQVRTLMKQDFAKVFEEYDAIVGPTA
PTTAFKLGEQLDDPLTMYANDIMTIPVNLVGIPAISVPAGLSEGLPVGLQIIGNYFDESTLYRVAHAFEQANGGFKMPQL
>Mature_479_residues
SLFDHGVAKLHTLLKEGEVTVTDLVKESFDRIEATDDQIGAFLTLNEDAFEQAKRMDELAKMSDNPLFGMPIGVKDNIVT
KGMRTTCASTFLENFVPAHDATVVERLHDAGALTIGKLNMDEFAMGSSNENSAFKPVRNPWDPTRVPGGSSGGSAASVAA
GQVLFSLGSDTGGSIRQPAAYCGVVGMKPTYGLVSRFGLVAFASSLDQIGPLTRTVEDNAYLLNTIAGHCDMDSTSAEVE
VPDYTAALNQDLTGMKFALPEEFMAEGVAPGVREQIEKAIETLKSLGATIETVSIPSVKYALSAYYLLASSEASSNLARF
DGIRYGRRAEADTLDEVFTYSREQGFGDEVKRRIMLGTYALSSGYYDAFYKKAQQVRTLMKQDFAKVFEEYDAIVGPTAP
TTAFKLGEQLDDPLTMYANDIMTIPVNLVGIPAISVPAGLSEGLPVGLQIIGNYFDESTLYRVAHAFEQANGGFKMPQL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=486, Percent_Identity=37.8600823045267, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.6619144602851, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI166795287, Length=215, Percent_Identity=29.7674418604651, Blast_Score=70, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=37.9545454545455, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=487, Percent_Identity=26.8993839835729, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17556264, Length=277, Percent_Identity=29.6028880866426, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=235, Percent_Identity=30.6382978723404, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=183, Percent_Identity=30.0546448087432, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=222, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=292, Percent_Identity=27.7397260273973, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=292, Percent_Identity=27.7397260273973, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=457, Percent_Identity=37.855579868709, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=28.6363636363636, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=439, Percent_Identity=25.5125284738041, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=454, Percent_Identity=40.7488986784141, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=27.4, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=27.4, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=27.4, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=27.4, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=28.8201160541586, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=25.7936507936508, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI45550774, Length=490, Percent_Identity=25.7142857142857, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=25.7142857142857, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=25.7142857142857, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=26.7281105990783, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=26.7281105990783, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=502, Percent_Identity=26.0956175298805, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_EXISA (C4L690)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885366.1
- GeneID:   7870912
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0993
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51829; Mature: 51698

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDHGVAKLHTLLKEGEVTVTDLVKESFDRIEATDDQIGAFLTLNEDAFEQAKRMDEL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHH
AKMSDNPLFGMPIGVKDNIVTKGMRTTCASTFLENFVPAHDATVVERLHDAGALTIGKLN
HHHCCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCC
MDEFAMGSSNENSAFKPVRNPWDPTRVPGGSSGGSAASVAAGQVLFSLGSDTGGSIRQPA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
AYCGVVGMKPTYGLVSRFGLVAFASSLDQIGPLTRTVEDNAYLLNTIAGHCDMDSTSAEV
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCEEEHHHHCCCCCCCCCCEE
EVPDYTAALNQDLTGMKFALPEEFMAEGVAPGVREQIEKAIETLKSLGATIETVSIPSVK
ECCCHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHH
YALSAYYLLASSEASSNLARFDGIRYGRRAEADTLDEVFTYSREQGFGDEVKRRIMLGTY
HHHHHHHHEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALSSGYYDAFYKKAQQVRTLMKQDFAKVFEEYDAIVGPTAPTTAFKLGEQLDDPLTMYAN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHC
DIMTIPVNLVGIPAISVPAGLSEGLPVGLQIIGNYFDESTLYRVAHAFEQANGGFKMPQL
CEEEECEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLFDHGVAKLHTLLKEGEVTVTDLVKESFDRIEATDDQIGAFLTLNEDAFEQAKRMDEL
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHH
AKMSDNPLFGMPIGVKDNIVTKGMRTTCASTFLENFVPAHDATVVERLHDAGALTIGKLN
HHHCCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCC
MDEFAMGSSNENSAFKPVRNPWDPTRVPGGSSGGSAASVAAGQVLFSLGSDTGGSIRQPA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
AYCGVVGMKPTYGLVSRFGLVAFASSLDQIGPLTRTVEDNAYLLNTIAGHCDMDSTSAEV
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCEEEHHHHCCCCCCCCCCEE
EVPDYTAALNQDLTGMKFALPEEFMAEGVAPGVREQIEKAIETLKSLGATIETVSIPSVK
ECCCHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHH
YALSAYYLLASSEASSNLARFDGIRYGRRAEADTLDEVFTYSREQGFGDEVKRRIMLGTY
HHHHHHHHEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ALSSGYYDAFYKKAQQVRTLMKQDFAKVFEEYDAIVGPTAPTTAFKLGEQLDDPLTMYAN
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHC
DIMTIPVNLVGIPAISVPAGLSEGLPVGLQIIGNYFDESTLYRVAHAFEQANGGFKMPQL
CEEEECEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA