Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 229916718

GI number: 229916718

Start: 932911

End: 933909

Strand: Reverse

Name: dagK [H]

Synonym: EAT1b_0991

Alternate gene names: 229916718

Gene position: 933909-932911 (Counterclockwise)

Preceding gene: 229916719

Following gene: 229916717

Centisome position: 31.13

GC content: 52.75

Gene sequence:

>999_bases
ATGCAACGGAAACGTGCGCGGGTCATTTATAACCCGACATCTGGTAAAGAAGTGATCAAACGGGAACTCCCGTACATTTT
GAACCGCCTCGAGGATGCGGGTTATGAGACGTCGACGTATGCGACGAAAGCGATTGGTGATGCCACGCTCGAAGCAAAAC
GGGCCTCAGCGGCCGAGTTCGACCTCATCATCGCCGCAGGCGGAGACGGGACATTGAACGAAGTCATCTCAGGGCTCGCC
CCGCTAGAGAATCGTCCGACGATCGGACTCATTCCGGTCGGGACGACGAACGACTTTGCCCGGGCGATGCGGATTCCACT
AAGTGTGGTCGGTGCACTCGACGTCATCTGTGACGGATTCGAGATGCCGGTCGACCTTGGGGAAATCGAAGGCGAGAAAG
GTGACATCCACTATTTCATCAATATCGCCGGTGGTGGTATCATGACCGAGCTCTCGTATGAAGTGCCATCGAAGTTGAAG
ACGGCACTCGGACAGCTCGCCTATTACGTGAAGGGAATGGAGAAACTACCACTCATCAAACCGACGTATATCGAACTTGA
GCACGATGAAGGAACGTTCCAAGGCGAGGTCATGGTCTTCCTCACATCGAACTCGAACTCGGTCGGTGGATTTGAAAAAA
TCTCACCACACGCGTCGTTGAATGACGGATTGTTTGATTTATTTATCCTTCGGAAATGTAACTTGTTCGAATTCATCCAC
GTCGTCCGCCTTCTCCTCCGGGGTGAGCATTTATCCAGCTCACTCGTCGAGCATGTGAAGACGAGCCGCGTCCGGATGAA
GACGACCGAGCCGATGAGTCTAAACATCGACGGCGAGTATGGCGGGGAGTGCACAGGTGAGATGCGGAACTTGTTCCGTC
ACTTGACGGTGCTCGCACCGAACGCTCGAATCCGCGAGATGGAAGATTTGAATGCACAATATGAACGCGAACAGATGATC
AAGCAATTGTTCAATGTTTCGCTCGATAAAGCAGAGTGA

Upstream 100 bases:

>100_bases
CGATGGCTTGTTCCACGAAGAGATCGCAAAACGCTAAGCAGGCGGCGAGACACTTGTGTCTCGCTTTTCGCTCGAGTTGA
GCATAGGGGAGAGAATCAAT

Downstream 100 bases:

>100_bases
AAAGATAGGAATGACGACATCAGTCATTCCTATTTGTAATGGAAGGAGCATTCGTATGCTACCAGTGAAAAAGAATGATC
GTCTCGATGTCCACGTCCAC

Product: diacylglycerol kinase catalytic region

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQRKRARVIYNPTSGKEVIKRELPYILNRLEDAGYETSTYATKAIGDATLEAKRASAAEFDLIIAAGGDGTLNEVISGLA
PLENRPTIGLIPVGTTNDFARAMRIPLSVVGALDVICDGFEMPVDLGEIEGEKGDIHYFINIAGGGIMTELSYEVPSKLK
TALGQLAYYVKGMEKLPLIKPTYIELEHDEGTFQGEVMVFLTSNSNSVGGFEKISPHASLNDGLFDLFILRKCNLFEFIH
VVRLLLRGEHLSSSLVEHVKTSRVRMKTTEPMSLNIDGEYGGECTGEMRNLFRHLTVLAPNARIREMEDLNAQYEREQMI
KQLFNVSLDKAE

Sequences:

>Translated_332_residues
MQRKRARVIYNPTSGKEVIKRELPYILNRLEDAGYETSTYATKAIGDATLEAKRASAAEFDLIIAAGGDGTLNEVISGLA
PLENRPTIGLIPVGTTNDFARAMRIPLSVVGALDVICDGFEMPVDLGEIEGEKGDIHYFINIAGGGIMTELSYEVPSKLK
TALGQLAYYVKGMEKLPLIKPTYIELEHDEGTFQGEVMVFLTSNSNSVGGFEKISPHASLNDGLFDLFILRKCNLFEFIH
VVRLLLRGEHLSSSLVEHVKTSRVRMKTTEPMSLNIDGEYGGECTGEMRNLFRHLTVLAPNARIREMEDLNAQYEREQMI
KQLFNVSLDKAE
>Mature_332_residues
MQRKRARVIYNPTSGKEVIKRELPYILNRLEDAGYETSTYATKAIGDATLEAKRASAAEFDLIIAAGGDGTLNEVISGLA
PLENRPTIGLIPVGTTNDFARAMRIPLSVVGALDVICDGFEMPVDLGEIEGEKGDIHYFINIAGGGIMTELSYEVPSKLK
TALGQLAYYVKGMEKLPLIKPTYIELEHDEGTFQGEVMVFLTSNSNSVGGFEKISPHASLNDGLFDLFILRKCNLFEFIH
VVRLLLRGEHLSSSLVEHVKTSRVRMKTTEPMSLNIDGEYGGECTGEMRNLFRHLTVLAPNARIREMEDLNAQYEREQMI
KQLFNVSLDKAE

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=252, Percent_Identity=29.7619047619048, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 36850; Mature: 36850

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKRARVIYNPTSGKEVIKRELPYILNRLEDAGYETSTYATKAIGDATLEAKRASAAEF
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCE
DLIIAAGGDGTLNEVISGLAPLENRPTIGLIPVGTTNDFARAMRIPLSVVGALDVICDGF
EEEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCHHHHHHHHHHHHCCC
EMPVDLGEIEGEKGDIHYFINIAGGGIMTELSYEVPSKLKTALGQLAYYVKGMEKLPLIK
CCCCCHHHCCCCCCCEEEEEEEECCCEEEEECHHCHHHHHHHHHHHHHHHHCHHHCCCCC
PTYIELEHDEGTFQGEVMVFLTSNSNSVGGFEKISPHASLNDGLFDLFILRKCNLFEFIH
CEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCHHHHHH
VVRLLLRGEHLSSSLVEHVKTSRVRMKTTEPMSLNIDGEYGGECTGEMRNLFRHLTVLAP
HHHHHHCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHCCC
NARIREMEDLNAQYEREQMIKQLFNVSLDKAE
CCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQRKRARVIYNPTSGKEVIKRELPYILNRLEDAGYETSTYATKAIGDATLEAKRASAAEF
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCE
DLIIAAGGDGTLNEVISGLAPLENRPTIGLIPVGTTNDFARAMRIPLSVVGALDVICDGF
EEEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCHHHHHHHHHHHHCCC
EMPVDLGEIEGEKGDIHYFINIAGGGIMTELSYEVPSKLKTALGQLAYYVKGMEKLPLIK
CCCCCHHHCCCCCCCEEEEEEEECCCEEEEECHHCHHHHHHHHHHHHHHHHCHHHCCCCC
PTYIELEHDEGTFQGEVMVFLTSNSNSVGGFEKISPHASLNDGLFDLFILRKCNLFEFIH
CEEEEEECCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCHHHHHH
VVRLLLRGEHLSSSLVEHVKTSRVRMKTTEPMSLNIDGEYGGECTGEMRNLFRHLTVLAP
HHHHHHCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHCCC
NARIREMEDLNAQYEREQMIKQLFNVSLDKAE
CCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]