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The map label for this gene is ygcA [C]

Identifier: 229916717

GI number: 229916717

Start: 931491

End: 932855

Strand: Reverse

Name: ygcA [C]

Synonym: EAT1b_0990

Alternate gene names: 229916717

Gene position: 932855-931491 (Counterclockwise)

Preceding gene: 229916718

Following gene: 229916716

Centisome position: 31.1

GC content: 54.29

Gene sequence:

>1365_bases
ATGCTACCAGTGAAAAAGAATGATCGTCTCGATGTCCACGTCCACGACCTGACCCATGAAGGGGCGGGTGTGGCACGTGT
TGACGGCTATACGTTGTTCGTGCCGAACGCGTTGCCGGGAGAGACGGTCGAAATCGTCGTGACGAAGACGAACAAGCAGT
ACGGGTTCGCCCGATTGTTGAACGTGAAAGAAGCGAGCGCGGACCGGGTCGAGCCACCGTGCCCGATCTTTTATCAATGT
GGCGGCTGTCAGCTGCAACACTTCAGTTATGAAGGGCAACTTCGCCAAAAACGAGAACGCGTCGTCGACGCGCTCAAGCG
TCTCGGTCAGTTCGACGTGCCGGTCCATGACACGATTGGGATGCCAGAACCTTGGCGCTACCGCAACAAGGCACAAGTCC
CGTTCAAACAAGAAGGGGACCGTCTCGTCTCAGGCTTTTATCGTCCTCGTTCGCATGATATCGTCGAGATGAGCGAATGT
CTGATTCAAGATGAGCGTAATGACAAGGCGATTCAAGTCGTCCGTGATGTGTTGGACGCACGTGGCGTACCGGCGTATGA
CGAGAAGACAGGACGTGGAGTTATCCGTCACGTCATGGCCCGTTACGGGTACCATTCGGGTGAACTGATGATCGTCCTGA
TCACGAAGACGACGAAACTAAAAGGCGTGAATGAGATCGTCGCCGATATCGTCGAACGTCTTCCGGACGTGACGTCGATC
ATGCAAAACGTCAACCCGGACAAGACGAACGTCATCCTCGGGGCGACGAACAAGTTGCTCTACGGCCACGAGACAATTGA
GGATAAAATCGCAGGACTCACGTTCACGATTTCTCCGCACTCGTTCTTCCAAGTCAACCCGGTTCAAACGGAGAAGCTGT
ACGGGAAGGCGTTAGAGTACGCACAACTGTCAGGTACGGAACAAGTCGTCGATGCCTATTGCGGTATCGGGACGATCAGC
CTTTTCCTCGCCCGGAAAGCGGCGCACGTGTATGGTGTTGAAGTCGTTCCAGAAGCAATCGAGGATGCGAAACAGAATGC
GCTCGTCAATGAGATCGACAATGTGACGTACGCATGTGGTGCAGCGGAAGATGTGCTCCCACAGTGGAAGAAAGATGGCG
TGAACCCGGACGTCATCGTCGTCGACCCACCACGTAAAGGCTGTGCCGAATCGTTCCTTGAGACGATGGTCGAGATGGGA
CCGAAGCGAATCGTATACGTCTCGTGTAATGTTGCGACACAAGCGCGTGATATGCGTTACCTCGCCGACCATGGCTATCG
CTTGATGGAAGTGACTCCGGTCGACATGTTCCCGCATACGGCGCATGTGGAAACGGTGGCGTGGTTGGAGAAAATTGAAA
AGTAA

Upstream 100 bases:

>100_bases
ATGATCAAGCAATTGTTCAATGTTTCGCTCGATAAAGCAGAGTGAAAAGATAGGAATGACGACATCAGTCATTCCTATTT
GTAATGGAAGGAGCATTCGT

Downstream 100 bases:

>100_bases
GCTTGAAAAGTACATGGTAGATAAGGGGTTTTTAAGGTTTTGGGATGGACTCAAGACTTTAAAAACCTCTTTTTATTTAT
TCATCGGAAATGTATTCAGA

Product: RNA methyltransferase, TrmA family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MLPVKKNDRLDVHVHDLTHEGAGVARVDGYTLFVPNALPGETVEIVVTKTNKQYGFARLLNVKEASADRVEPPCPIFYQC
GGCQLQHFSYEGQLRQKRERVVDALKRLGQFDVPVHDTIGMPEPWRYRNKAQVPFKQEGDRLVSGFYRPRSHDIVEMSEC
LIQDERNDKAIQVVRDVLDARGVPAYDEKTGRGVIRHVMARYGYHSGELMIVLITKTTKLKGVNEIVADIVERLPDVTSI
MQNVNPDKTNVILGATNKLLYGHETIEDKIAGLTFTISPHSFFQVNPVQTEKLYGKALEYAQLSGTEQVVDAYCGIGTIS
LFLARKAAHVYGVEVVPEAIEDAKQNALVNEIDNVTYACGAAEDVLPQWKKDGVNPDVIVVDPPRKGCAESFLETMVEMG
PKRIVYVSCNVATQARDMRYLADHGYRLMEVTPVDMFPHTAHVETVAWLEKIEK

Sequences:

>Translated_454_residues
MLPVKKNDRLDVHVHDLTHEGAGVARVDGYTLFVPNALPGETVEIVVTKTNKQYGFARLLNVKEASADRVEPPCPIFYQC
GGCQLQHFSYEGQLRQKRERVVDALKRLGQFDVPVHDTIGMPEPWRYRNKAQVPFKQEGDRLVSGFYRPRSHDIVEMSEC
LIQDERNDKAIQVVRDVLDARGVPAYDEKTGRGVIRHVMARYGYHSGELMIVLITKTTKLKGVNEIVADIVERLPDVTSI
MQNVNPDKTNVILGATNKLLYGHETIEDKIAGLTFTISPHSFFQVNPVQTEKLYGKALEYAQLSGTEQVVDAYCGIGTIS
LFLARKAAHVYGVEVVPEAIEDAKQNALVNEIDNVTYACGAAEDVLPQWKKDGVNPDVIVVDPPRKGCAESFLETMVEMG
PKRIVYVSCNVATQARDMRYLADHGYRLMEVTPVDMFPHTAHVETVAWLEKIEK
>Mature_454_residues
MLPVKKNDRLDVHVHDLTHEGAGVARVDGYTLFVPNALPGETVEIVVTKTNKQYGFARLLNVKEASADRVEPPCPIFYQC
GGCQLQHFSYEGQLRQKRERVVDALKRLGQFDVPVHDTIGMPEPWRYRNKAQVPFKQEGDRLVSGFYRPRSHDIVEMSEC
LIQDERNDKAIQVVRDVLDARGVPAYDEKTGRGVIRHVMARYGYHSGELMIVLITKTTKLKGVNEIVADIVERLPDVTSI
MQNVNPDKTNVILGATNKLLYGHETIEDKIAGLTFTISPHSFFQVNPVQTEKLYGKALEYAQLSGTEQVVDAYCGIGTIS
LFLARKAAHVYGVEVVPEAIEDAKQNALVNEIDNVTYACGAAEDVLPQWKKDGVNPDVIVVDPPRKGCAESFLETMVEMG
PKRIVYVSCNVATQARDMRYLADHGYRLMEVTPVDMFPHTAHVETVAWLEKIEK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=202, Percent_Identity=39.1089108910891, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI51173878, Length=202, Percent_Identity=39.1089108910891, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI269784680, Length=269, Percent_Identity=28.6245353159851, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI269784676, Length=269, Percent_Identity=28.6245353159851, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI20149688, Length=269, Percent_Identity=28.6245353159851, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI269784678, Length=269, Percent_Identity=28.6245353159851, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1789148, Length=448, Percent_Identity=34.8214285714286, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1787083, Length=377, Percent_Identity=25.9946949602122, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1790403, Length=177, Percent_Identity=31.0734463276836, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=166, Percent_Identity=34.3373493975904, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=443, Percent_Identity=25.7336343115124, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24666579, Length=416, Percent_Identity=28.6057692307692, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 50905; Mature: 50905

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVKKNDRLDVHVHDLTHEGAGVARVDGYTLFVPNALPGETVEIVVTKTNKQYGFARLL
CCCCCCCCCEEEEEEECCCCCCCEEEECCEEEEECCCCCCCEEEEEEEECCCCCCHHHHH
NVKEASADRVEPPCPIFYQCGGCQLQHFSYEGQLRQKRERVVDALKRLGQFDVPVHDTIG
CCHHCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
MPEPWRYRNKAQVPFKQEGDRLVSGFYRPRSHDIVEMSECLIQDERNDKAIQVVRDVLDA
CCCCCCCCCCCCCCCHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
RGVPAYDEKTGRGVIRHVMARYGYHSGELMIVLITKTTKLKGVNEIVADIVERLPDVTSI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHH
MQNVNPDKTNVILGATNKLLYGHETIEDKIAGLTFTISPHSFFQVNPVQTEKLYGKALEY
HHCCCCCCCEEEEECCCCEEECCHHHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHHHH
AQLSGTEQVVDAYCGIGTISLFLARKAAHVYGVEVVPEAIEDAKQNALVNEIDNVTYACG
HHCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEECC
AAEDVLPQWKKDGVNPDVIVVDPPRKGCAESFLETMVEMGPKRIVYVSCNVATQARDMRY
CHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
LADHGYRLMEVTPVDMFPHTAHVETVAWLEKIEK
HHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLPVKKNDRLDVHVHDLTHEGAGVARVDGYTLFVPNALPGETVEIVVTKTNKQYGFARLL
CCCCCCCCCEEEEEEECCCCCCCEEEECCEEEEECCCCCCCEEEEEEEECCCCCCHHHHH
NVKEASADRVEPPCPIFYQCGGCQLQHFSYEGQLRQKRERVVDALKRLGQFDVPVHDTIG
CCHHCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
MPEPWRYRNKAQVPFKQEGDRLVSGFYRPRSHDIVEMSECLIQDERNDKAIQVVRDVLDA
CCCCCCCCCCCCCCCHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
RGVPAYDEKTGRGVIRHVMARYGYHSGELMIVLITKTTKLKGVNEIVADIVERLPDVTSI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHH
MQNVNPDKTNVILGATNKLLYGHETIEDKIAGLTFTISPHSFFQVNPVQTEKLYGKALEY
HHCCCCCCCEEEEECCCCEEECCHHHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHHHH
AQLSGTEQVVDAYCGIGTISLFLARKAAHVYGVEVVPEAIEDAKQNALVNEIDNVTYACG
HHCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEECC
AAEDVLPQWKKDGVNPDVIVVDPPRKGCAESFLETMVEMGPKRIVYVSCNVATQARDMRY
CHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
LADHGYRLMEVTPVDMFPHTAHVETVAWLEKIEK
HHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]