| Definition | Exiguobacterium sp. AT1b, complete genome. |
|---|---|
| Accession | NC_012673 |
| Length | 2,999,895 |
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The map label for this gene is polA [H]
Identifier: 229916392
GI number: 229916392
Start: 611700
End: 614294
Strand: Reverse
Name: polA [H]
Synonym: EAT1b_0662
Alternate gene names: 229916392
Gene position: 614294-611700 (Counterclockwise)
Preceding gene: 229916393
Following gene: 229916391
Centisome position: 20.48
GC content: 47.51
Gene sequence:
>2595_bases ATGAATAAACTACTAGTCCTAGATGGGAACTCCTTAACATATCGTGCGTTTTTTGCACTGCCTCCGATGTCGGATGCGTC AGGTCGGAATACGAATGCAGTGTATGGGTTTACGATGATGTTATTGAAACTAATGGAAGATGAGCAACCGACTCATTTTG CCGTGGCGTTCGATGCGTCAAAGAAAACGTTCCGTCACGATACGTTCGCAGAATATAAAGGTGGACGTCAAAAGACACCG GGAGAATTGCGTGAACAATTCCCACTCGTGCGTGACGTTTGTCGGGCGTTTGGCATCTCTGTCTTAGAGCTCGATCAATA CGAGGCGGATGATATCATCGGGACGCTCTCAAAAGATCCGACTTTTGATCAAGTGACGGTTGTGACGGGGGATAAGGATT TACTTCAATTGATCAATGAACGTGTGACGGTCTATTTGACGAAACGAGGCATCACCGAAGTCGAGAAAATGACCGAGACC GCGTTCAAGGAACGCTACGACGGACTACAACCGATTCAGATGATTGACCTAAAAGGATTGATGGGGGATAAATCCGACAA CATCCCTGGGATTCCAGGGGTCGGGGAAAAGACGGCACTGAAGTTGATTGCCACATACGGTACCGTCGAGGCCCTTTATG AACATACGGATGAGTTGAAAGGGAAGCAAAAGGAAAAAGTAGTGGCGAATGAACAAGAGGCGAAAATGTCGAAACAGTTG GCGACCATTTACACAGATGTACCGATTGATGTGACGATGGATGAACTTGTCTTTAAGCCATATGACGCTTCGGTCGTCAA AACGCTATTTATGGATTTACAGTTCAAGTCACTCATCGGCAAACTGGCGGATACCTCTTCAGAAGAACCGGTTGAAAAAG CGGAGCGACCAACAATCGCGTTGGTCGATCAAGATTTGTCACGTGCTGTGTTGATGCTTGAACAACTGCATGATGATTAT TTTGAAGAAGACGTGATTGGAGTGGCGATCGCGTCGGAGCGAGGTGTCACGATTGGAGATGTGACGCTTCTTGAGGACGA ACGGGTCAAGGAATGGATTGAAGACGAGACGAAGACGACGATTTGTCTCGATGCGAAACAGACAATCATTCAATTGAAAC GTCATCAACTGGCACTGCGTGGGTATGAAGACTTGCTTGTCGCAGGGTATTTGCTCAACCTATCTGGCGGGACATCTCTC AGCTCGATTGCCGCACACTTTACGTATCCTCTTGAGGAAGATGAAACGGTCTACGGAAAAGGTGCAAAAATCAAGGTGCC GGAAGAGAACGTCTTTCATGCACATCTCGCAGAAAAGGCGTACGCCATCTACACCTTGTTTGAACGTGTGGTTTCGGAGT TGAAAGAGAATGAACAGACAGAACTCTATGAGGCGCTTGAAAAACCACTCGTTTCCGTATTGGCCGAGATGGAGTGGGCC GGTATCCACGTAAATGTCGAAACCCTTCAACATATGGAAACGGACCTACGTGAACGTCTAAACGCACTCGAGATCACGAT TCATGAATTAGCCGGCGAACCGTTCAACATCAACTCACCGAAACAGTTAGGGGTCGTCCTGTTTGAAAAACTGTCACTCC CTCCTGTGAAAAAGACGAAAACAGGATACTCAACGGCAGCGGATGTCCTCGAGAAACTGGCGCCGCTTCATCCGATCATC GAGAAAATCATGCATTATCGTGAACTTGGCAAGCTTCAGTCCACGTATGTCGAAGGCTTGCAGAAAGTCGTGAAGGATGA TGGCAAGATTCATACGCGTTATACACAGACGCTAACTCAAACGGGACGGCTTTCGTCGGTTAACCCGAACCTTCAAAATA TCCCGATTCGATTGGAAGAAGGGCGTCGTATCCGTAAAGCGTTCACCGCGAGTGAACCGGGATGGGTCCTTTATGCGGTC GATTACTCCCAAATCGAACTGCGCATCATGGCACATATGTCACAAGATGAAAAGATGATGGCAGCCTTCCTTCACGATGA GGATATTCATACGTCGACGGCGGCGAACGTGTTTAAAGTTTCCGCTGAAGAAGTCACGTCCCTCATGCGTCGTCAAGCGA AAGCCGTCAACTTTGGAATCATCTATGGCATCAGTGATTATGGACTCTCGCAAAACCTAGGGATTACACGAAAAGAAGCA CAGACCTTTATCGATACGTACTTTGAACAGTTCCCTGGAGTGAAGACGTTCATGGATGCCGCGATTGAACGGGCACGTGA ACACGGGTTCGTCGAGACGATGTTGAAACGACGCCGGAACATCCCGGATATTCATTCTCGCAATTTCAACTTACGTGGGT TTGCTGAACGTACCGCAATCAACACACCAATTCAAGGAACGGCAGCGGACATTATTAAAAAAGCGATGATTGATGTCGAT CATGCGTTAAACGCGTCAAAACTTCAATCCAAGTTGTTGTTACAAGTACACGATGAATTGATATTCGAAGGTCCGACCGA AGAGATGGAAGCATTAGAAAAACTCGTGACCGAAGCGATGGAACAAACGGTCACACTTGATGTGCCGTTACGTGCGGACG GAGCGGTTGGCACGACGTGGTTTGATACGAAATAA
Upstream 100 bases:
>100_bases GAGAATTTGGCCCTTTTCTATTCGTAGAAAAGGGTCTTTTTTTTCGAACGCATGTACCCGTATGGTATGATGGTTGAAGA GAAGAATGGAGGTCGATTGA
Downstream 100 bases:
>100_bases ACGGATAGAAAGGAAATCAGTATGCCAGAATTACCAGAAGTCGAAACGGTCTGTCGCAGGTTACGACCTGCCGTTTCAGG AAAAACGATTCAGTCTGTCG
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 864; Mature: 864
Protein sequence:
>864_residues MNKLLVLDGNSLTYRAFFALPPMSDASGRNTNAVYGFTMMLLKLMEDEQPTHFAVAFDASKKTFRHDTFAEYKGGRQKTP GELREQFPLVRDVCRAFGISVLELDQYEADDIIGTLSKDPTFDQVTVVTGDKDLLQLINERVTVYLTKRGITEVEKMTET AFKERYDGLQPIQMIDLKGLMGDKSDNIPGIPGVGEKTALKLIATYGTVEALYEHTDELKGKQKEKVVANEQEAKMSKQL ATIYTDVPIDVTMDELVFKPYDASVVKTLFMDLQFKSLIGKLADTSSEEPVEKAERPTIALVDQDLSRAVLMLEQLHDDY FEEDVIGVAIASERGVTIGDVTLLEDERVKEWIEDETKTTICLDAKQTIIQLKRHQLALRGYEDLLVAGYLLNLSGGTSL SSIAAHFTYPLEEDETVYGKGAKIKVPEENVFHAHLAEKAYAIYTLFERVVSELKENEQTELYEALEKPLVSVLAEMEWA GIHVNVETLQHMETDLRERLNALEITIHELAGEPFNINSPKQLGVVLFEKLSLPPVKKTKTGYSTAADVLEKLAPLHPII EKIMHYRELGKLQSTYVEGLQKVVKDDGKIHTRYTQTLTQTGRLSSVNPNLQNIPIRLEEGRRIRKAFTASEPGWVLYAV DYSQIELRIMAHMSQDEKMMAAFLHDEDIHTSTAANVFKVSAEEVTSLMRRQAKAVNFGIIYGISDYGLSQNLGITRKEA QTFIDTYFEQFPGVKTFMDAAIERAREHGFVETMLKRRRNIPDIHSRNFNLRGFAERTAINTPIQGTAADIIKKAMIDVD HALNASKLQSKLLLQVHDELIFEGPTEEMEALEKLVTEAMEQTVTLDVPLRADGAVGTTWFDTK
Sequences:
>Translated_864_residues MNKLLVLDGNSLTYRAFFALPPMSDASGRNTNAVYGFTMMLLKLMEDEQPTHFAVAFDASKKTFRHDTFAEYKGGRQKTP GELREQFPLVRDVCRAFGISVLELDQYEADDIIGTLSKDPTFDQVTVVTGDKDLLQLINERVTVYLTKRGITEVEKMTET AFKERYDGLQPIQMIDLKGLMGDKSDNIPGIPGVGEKTALKLIATYGTVEALYEHTDELKGKQKEKVVANEQEAKMSKQL ATIYTDVPIDVTMDELVFKPYDASVVKTLFMDLQFKSLIGKLADTSSEEPVEKAERPTIALVDQDLSRAVLMLEQLHDDY FEEDVIGVAIASERGVTIGDVTLLEDERVKEWIEDETKTTICLDAKQTIIQLKRHQLALRGYEDLLVAGYLLNLSGGTSL SSIAAHFTYPLEEDETVYGKGAKIKVPEENVFHAHLAEKAYAIYTLFERVVSELKENEQTELYEALEKPLVSVLAEMEWA GIHVNVETLQHMETDLRERLNALEITIHELAGEPFNINSPKQLGVVLFEKLSLPPVKKTKTGYSTAADVLEKLAPLHPII EKIMHYRELGKLQSTYVEGLQKVVKDDGKIHTRYTQTLTQTGRLSSVNPNLQNIPIRLEEGRRIRKAFTASEPGWVLYAV DYSQIELRIMAHMSQDEKMMAAFLHDEDIHTSTAANVFKVSAEEVTSLMRRQAKAVNFGIIYGISDYGLSQNLGITRKEA QTFIDTYFEQFPGVKTFMDAAIERAREHGFVETMLKRRRNIPDIHSRNFNLRGFAERTAINTPIQGTAADIIKKAMIDVD HALNASKLQSKLLLQVHDELIFEGPTEEMEALEKLVTEAMEQTVTLDVPLRADGAVGTTWFDTK >Mature_864_residues MNKLLVLDGNSLTYRAFFALPPMSDASGRNTNAVYGFTMMLLKLMEDEQPTHFAVAFDASKKTFRHDTFAEYKGGRQKTP GELREQFPLVRDVCRAFGISVLELDQYEADDIIGTLSKDPTFDQVTVVTGDKDLLQLINERVTVYLTKRGITEVEKMTET AFKERYDGLQPIQMIDLKGLMGDKSDNIPGIPGVGEKTALKLIATYGTVEALYEHTDELKGKQKEKVVANEQEAKMSKQL ATIYTDVPIDVTMDELVFKPYDASVVKTLFMDLQFKSLIGKLADTSSEEPVEKAERPTIALVDQDLSRAVLMLEQLHDDY FEEDVIGVAIASERGVTIGDVTLLEDERVKEWIEDETKTTICLDAKQTIIQLKRHQLALRGYEDLLVAGYLLNLSGGTSL SSIAAHFTYPLEEDETVYGKGAKIKVPEENVFHAHLAEKAYAIYTLFERVVSELKENEQTELYEALEKPLVSVLAEMEWA GIHVNVETLQHMETDLRERLNALEITIHELAGEPFNINSPKQLGVVLFEKLSLPPVKKTKTGYSTAADVLEKLAPLHPII EKIMHYRELGKLQSTYVEGLQKVVKDDGKIHTRYTQTLTQTGRLSSVNPNLQNIPIRLEEGRRIRKAFTASEPGWVLYAV DYSQIELRIMAHMSQDEKMMAAFLHDEDIHTSTAANVFKVSAEEVTSLMRRQAKAVNFGIIYGISDYGLSQNLGITRKEA QTFIDTYFEQFPGVKTFMDAAIERAREHGFVETMLKRRRNIPDIHSRNFNLRGFAERTAINTPIQGTAADIIKKAMIDVD HALNASKLQSKLLLQVHDELIFEGPTEEMEALEKLVTEAMEQTVTLDVPLRADGAVGTTWFDTK
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=477, Percent_Identity=34.5911949685535, Blast_Score=251, Evalue=3e-66, Organism=Homo sapiens, GI139394648, Length=603, Percent_Identity=27.1973466003317, Blast_Score=207, Evalue=3e-53, Organism=Escherichia coli, GI1790294, Length=936, Percent_Identity=37.8205128205128, Blast_Score=547, Evalue=1e-156, Organism=Escherichia coli, GI226510970, Length=240, Percent_Identity=30, Blast_Score=116, Evalue=7e-27, Organism=Caenorhabditis elegans, GI86563272, Length=380, Percent_Identity=29.7368421052632, Blast_Score=169, Evalue=7e-42, Organism=Drosophila melanogaster, GI17933644, Length=430, Percent_Identity=30.9302325581395, Blast_Score=212, Evalue=9e-55,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 97295; Mature: 97295
Theoretical pI: Translated: 4.89; Mature: 4.89
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNKLLVLDGNSLTYRAFFALPPMSDASGRNTNAVYGFTMMLLKLMEDEQPTHFAVAFDAS CCCEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC KKTFRHDTFAEYKGGRQKTPGELREQFPLVRDVCRAFGISVLELDQYEADDIIGTLSKDP CHHHCCCCHHHHCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCC TFDQVTVVTGDKDLLQLINERVTVYLTKRGITEVEKMTETAFKERYDGLQPIQMIDLKGL CCCEEEEEECCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHEEHHHH MGDKSDNIPGIPGVGEKTALKLIATYGTVEALYEHTDELKGKQKEKVVANEQEAKMSKQL CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHH ATIYTDVPIDVTMDELVFKPYDASVVKTLFMDLQFKSLIGKLADTSSEEPVEKAERPTIA HHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEE LVDQDLSRAVLMLEQLHDDYFEEDVIGVAIASERGVTIGDVTLLEDERVKEWIEDETKTT EECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEECHHHHHHHHHCCCCEE ICLDAKQTIIQLKRHQLALRGYEDLLVAGYLLNLSGGTSLSSIAAHFTYPLEEDETVYGK EEECHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCEEECC GAKIKVPEENVFHAHLAEKAYAIYTLFERVVSELKENEQTELYEALEKPLVSVLAEMEWA CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC GIHVNVETLQHMETDLRERLNALEITIHELAGEPFNINSPKQLGVVLFEKLSLPPVKKTK EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC TGYSTAADVLEKLAPLHPIIEKIMHYRELGKLQSTYVEGLQKVVKDDGKIHTRYTQTLTQ CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH TGRLSSVNPNLQNIPIRLEEGRRIRKAFTASEPGWVLYAVDYSQIELRIMAHMSQDEKMM CCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEEEEEHHHEEEHHHHHCCCCHHHH AAFLHDEDIHTSTAANVFKVSAEEVTSLMRRQAKAVNFGIIYGISDYGLSQNLGITRKEA HHHHCCCCCCCHHHHHHEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHH QTFIDTYFEQFPGVKTFMDAAIERAREHGFVETMLKRRRNIPDIHSRNFNLRGFAERTAI HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHC NTPIQGTAADIIKKAMIDVDHALNASKLQSKLLLQVHDELIFEGPTEEMEALEKLVTEAM CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH EQTVTLDVPLRADGAVGTTWFDTK HCEEEEEECCCCCCCCCCCCCCCC >Mature Secondary Structure MNKLLVLDGNSLTYRAFFALPPMSDASGRNTNAVYGFTMMLLKLMEDEQPTHFAVAFDAS CCCEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC KKTFRHDTFAEYKGGRQKTPGELREQFPLVRDVCRAFGISVLELDQYEADDIIGTLSKDP CHHHCCCCHHHHCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCC TFDQVTVVTGDKDLLQLINERVTVYLTKRGITEVEKMTETAFKERYDGLQPIQMIDLKGL CCCEEEEEECCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHEEHHHH MGDKSDNIPGIPGVGEKTALKLIATYGTVEALYEHTDELKGKQKEKVVANEQEAKMSKQL CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHH ATIYTDVPIDVTMDELVFKPYDASVVKTLFMDLQFKSLIGKLADTSSEEPVEKAERPTIA HHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEE LVDQDLSRAVLMLEQLHDDYFEEDVIGVAIASERGVTIGDVTLLEDERVKEWIEDETKTT EECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEECHHHHHHHHHCCCCEE ICLDAKQTIIQLKRHQLALRGYEDLLVAGYLLNLSGGTSLSSIAAHFTYPLEEDETVYGK EEECHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCEEECC GAKIKVPEENVFHAHLAEKAYAIYTLFERVVSELKENEQTELYEALEKPLVSVLAEMEWA CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC GIHVNVETLQHMETDLRERLNALEITIHELAGEPFNINSPKQLGVVLFEKLSLPPVKKTK EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC TGYSTAADVLEKLAPLHPIIEKIMHYRELGKLQSTYVEGLQKVVKDDGKIHTRYTQTLTQ CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH TGRLSSVNPNLQNIPIRLEEGRRIRKAFTASEPGWVLYAVDYSQIELRIMAHMSQDEKMM CCCCCCCCCCCCCCCEEECCCCHHHHHHCCCCCCEEEEEEEHHHEEEHHHHHCCCCHHHH AAFLHDEDIHTSTAANVFKVSAEEVTSLMRRQAKAVNFGIIYGISDYGLSQNLGITRKEA HHHHCCCCCCCHHHHHHEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHH QTFIDTYFEQFPGVKTFMDAAIERAREHGFVETMLKRRRNIPDIHSRNFNLRGFAERTAI HHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHC NTPIQGTAADIIKKAMIDVDHALNASKLQSKLLLQVHDELIFEGPTEEMEALEKLVTEAM CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH EQTVTLDVPLRADGAVGTTWFDTK HCEEEEEECCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]