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The map label for this gene is mutM

Identifier: 229916391

GI number: 229916391

Start: 610851

End: 611678

Strand: Reverse

Name: mutM

Synonym: EAT1b_0661

Alternate gene names: 229916391

Gene position: 611678-610851 (Counterclockwise)

Preceding gene: 229916392

Following gene: 229916390

Centisome position: 20.39

GC content: 50.36

Gene sequence:

>828_bases
ATGCCAGAATTACCAGAAGTCGAAACGGTCTGTCGCAGGTTACGACCTGCCGTTTCAGGAAAAACGATTCAGTCTGTCGA
TGTCCTCGACCCAAAAATTATCCGCGGGCTCGACCCCGAAGAATGGGTTCACCATTTAATCGGAGAAACGATTACGGATG
TGGAACGTCGGGGGAAATTCATCTTATTCAAATTGACGAACGGGTATCTCGTCTCCCATCTTCGCATGGAAGGGAAGTTT
TTCCCTTATGAGACTGCTACAGAACCCGTGAAACATACACATGTCGTGATTACGTTTACGGACCAATCGACTCTACACTA
TAATGATGTTCGTAAATTCGGTACGATGGAGTTGCGGACGAATGAAACGATCCATACCACGCCACCGCTCTCATTACTTG
CGTATGAGCCGTTTGACGAGCGTGTGACAACCGAGGCGTTACACCGACGTTTAGAGCGCATGAAGACACGCGCGATTAAG
ACGGCACTCCTCGACCAGTCCATCTTTGTCGGATTAGGAAACATCTATGTAGATGAGACGCTGTTTCGTGCGGGTGTCCA
TCCGACTCGACCGGCCGCAAGTTTATCACGTGAAGAAGTCGACCGTGTGCGGACTGAAGCCGTTGCCGTCTTGAATGAGG
CCATTGAGCGTGGAGGTAGTACGATTCGAAGTTATGCCGATCCAGATGGCGCCACAGGAACGTTTCAAGAGAGGCTCTAC
GTGTATGGACAGACCGGAGAACCGTGCCGTCGATGCGGACATGAAATTGAAAAGATGAAATTGGGCGGGCGAGGCACGCA
TTACTGTCCGCACTGTCAACAACGTTAA

Upstream 100 bases:

>100_bases
GATGGAACAAACGGTCACACTTGATGTGCCGTTACGTGCGGACGGAGCGGTTGGCACGACGTGGTTTGATACGAAATAAA
CGGATAGAAAGGAAATCAGT

Downstream 100 bases:

>100_bases
GGGGTGGGCGTGTATGTTGAAGATTGGTTTGACTGGTGGGATCGCAACCGGAAAGTCTACGGTATCGCGACTGTTTCGAG
AGCGAGGTATTCCGATTATC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPELPEVETVCRRLRPAVSGKTIQSVDVLDPKIIRGLDPEEWVHHLIGETITDVERRGKFILFKLTNGYLVSHLRMEGKF
FPYETATEPVKHTHVVITFTDQSTLHYNDVRKFGTMELRTNETIHTTPPLSLLAYEPFDERVTTEALHRRLERMKTRAIK
TALLDQSIFVGLGNIYVDETLFRAGVHPTRPAASLSREEVDRVRTEAVAVLNEAIERGGSTIRSYADPDGATGTFQERLY
VYGQTGEPCRRCGHEIEKMKLGGRGTHYCPHCQQR

Sequences:

>Translated_275_residues
MPELPEVETVCRRLRPAVSGKTIQSVDVLDPKIIRGLDPEEWVHHLIGETITDVERRGKFILFKLTNGYLVSHLRMEGKF
FPYETATEPVKHTHVVITFTDQSTLHYNDVRKFGTMELRTNETIHTTPPLSLLAYEPFDERVTTEALHRRLERMKTRAIK
TALLDQSIFVGLGNIYVDETLFRAGVHPTRPAASLSREEVDRVRTEAVAVLNEAIERGGSTIRSYADPDGATGTFQERLY
VYGQTGEPCRRCGHEIEKMKLGGRGTHYCPHCQQR
>Mature_274_residues
PELPEVETVCRRLRPAVSGKTIQSVDVLDPKIIRGLDPEEWVHHLIGETITDVERRGKFILFKLTNGYLVSHLRMEGKFF
PYETATEPVKHTHVVITFTDQSTLHYNDVRKFGTMELRTNETIHTTPPLSLLAYEPFDERVTTEALHRRLERMKTRAIKT
ALLDQSIFVGLGNIYVDETLFRAGVHPTRPAASLSREEVDRVRTEAVAVLNEAIERGGSTIRSYADPDGATGTFQERLYV
YGQTGEPCRRCGHEIEKMKLGGRGTHYCPHCQQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=274, Percent_Identity=37.956204379562, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786932, Length=278, Percent_Identity=30.9352517985612, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_EXISA (C4L470)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885037.1
- GeneID:   7871433
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0661
- OMA:   RSTFYCA
- ProtClustDB:   PRK01103
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31315; Mature: 31184

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 59-59 ACT_SITE 264-264 BINDING 92-92 BINDING 111-111 BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVCRRLRPAVSGKTIQSVDVLDPKIIRGLDPEEWVHHLIGETITDVERRGKF
CCCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCE
ILFKLTNGYLVSHLRMEGKFFPYETATEPVKHTHVVITFTDQSTLHYNDVRKFGTMELRT
EEEEECCCEEEEHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHCCEEEEC
NETIHTTPPLSLLAYEPFDERVTTEALHRRLERMKTRAIKTALLDQSIFVGLGNIYVDET
CCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LFRAGVHPTRPAASLSREEVDRVRTEAVAVLNEAIERGGSTIRSYADPDGATGTFQERLY
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEECEEE
VYGQTGEPCRRCGHEIEKMKLGGRGTHYCPHCQQR
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETVCRRLRPAVSGKTIQSVDVLDPKIIRGLDPEEWVHHLIGETITDVERRGKF
CCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCE
ILFKLTNGYLVSHLRMEGKFFPYETATEPVKHTHVVITFTDQSTLHYNDVRKFGTMELRT
EEEEECCCEEEEHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHCCEEEEC
NETIHTTPPLSLLAYEPFDERVTTEALHRRLERMKTRAIKTALLDQSIFVGLGNIYVDET
CCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LFRAGVHPTRPAASLSREEVDRVRTEAVAVLNEAIERGGSTIRSYADPDGATGTFQERLY
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEECEEE
VYGQTGEPCRRCGHEIEKMKLGGRGTHYCPHCQQR
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA