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The map label for this gene is dnaX [H]

Identifier: 229587233

GI number: 229587233

Start: 1249861

End: 1251462

Strand: Direct

Name: dnaX [H]

Synonym: RAF_ORF1223

Alternate gene names: 229587233

Gene position: 1249861-1251462 (Clockwise)

Preceding gene: 229587232

Following gene: 229587234

Centisome position: 97.76

GC content: 31.59

Gene sequence:

>1602_bases
ATGTCATTCCCGCGAAAGCAGGAATTCTCAGTAAAACATATACAAAATAAATTTTTTAGTCTTATAACTATCGTGCTAGA
TATAAATTCATCAAATCAATATATTCCTTTTGCAAGGAAATATCGTCCTAGTAACTTTGCCGAGCTTCAGGGGCAAGAGG
TATTAGTCAAAGTTTTAAGCTATACTATTTTAAACGATAGGCTCGCCGGAGGCTATCTTTTAACAGGTATTAGAGGAGTC
GGTAAAACTACTTCCGCCCGAATTATTGCTAAAGCCGTCAACTGTTCTGCTTTAATTACCGAAAATACGACTATTAAAAC
ATGCGAGCAATGTACGAATTGCATCAGCTTTAATAATCATAATCACCCTGATATAATCGAAATTGATGCGGCAAGTAAAA
CTAGCGTAGATGACATACGTAGAATTATAGAATCCGCCGAGTATAAGCCCCTACAAGGTAAGCATAAAATCTTCATTATT
GATGAGGTGCATATGCTCTCTAAAGGGGCATTTAATGCACTTCTTAAGACACTGGAAGAACCACCACCTCATGTAATATT
TATCTTTGCAACGACGGAAGTACAAAAGATACCTGCAACTATTATCTCAAGATGTCAACGTTATGATTTAAGGCGGTTGA
GTTTTGAAGAGATTTTTAAGCTACTTGAATATATAACTAAGCAAGAAAATTTAAAAACCGATATAGAAGCACTAAGAATT
ATAGCTTACAAATCGGAAGGGTCGGCACGTGATGCAGTCTCTATTTTAGATCAAGCAGCTAGTATGTCGGTGAAATCTGA
CAATATAATTAGTCCTCAAGTAATTAATCAAATGCTTGGGCTTGTCGATAGTTCGGTTATAATAGAATTTGTCGAATGTA
TTATCTACAGAGAGACGGAAAAAGCTATAAATTTAATAAATAAACTTTACGGTTCTTCGGTTAATCTTGAGATTTTTATA
GAATCGGCAGCAGATTTTATTGCTTATCTTAATAAAGTAAAAATGTTACCTAATTATAGTTTGCCTATATATGAATCATT
TAATGATAGAACTAAAAACATATTAGATAAAACCAGCTTGCCGCACTTATCAATCTTATGGCAAATATATAATAAGGGAG
TAGGGGAAATAAAAATTTCCTATAACCAACTAACAGAAACGGAAATGTTAGTTATAAAATCTATATATTCGACGTCGCTA
CCCTTGCTTACAGATTTTGACGGTAATAATCAAAATTTAAATCAATCAATTAATCCGGAAATAAAAAAAAAATTTGAAAT
TGTAGATTTTCTTGAATATTTATATAAAAATAATGAGATAGATATATATTATTTCCTGCTCAATCATACGGAACTTAAAA
ATCTCAGTAATAATAGATTAGAGTTCGTAAGTCTTGAAGTTACAAGCAAAATAAAAAGACAAATAGACGATTTGTTAGCT
GCTTTTACTAATGAAAAGTTTGAGATAGTAATAATAAAAGAGCAGAACAAGCAAACCTTAAAGAATCAGTTAATTGGTAA
GATTGAAGCAAGTAACGATTTTGGTTTAATTAAAAAACATTTCCCAAATATAACAATTTCGGATATTTTACTTAAAAGCT
AA

Upstream 100 bases:

>100_bases
CGGTCAGGTCTGAAAGGAAGCAGCCAGAGTGGGATTCGATGGGTCATTACTAGCATTATTCTAATTTTGATGTCATTTCT
ACAGCAAGGCGGCTCGTTTT

Downstream 100 bases:

>100_bases
AAATATATAGGATAGAATTATGGTAAATTTTAATCAGTTTTTAAAGCAAGCCCAATCAATGCAAAAGAAAATGCAAGAAG
CTCAAGAGCAAATGGCAAAT

Product: DNA polymerase III subunits gamma and tau

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSFPRKQEFSVKHIQNKFFSLITIVLDINSSNQYIPFARKYRPSNFAELQGQEVLVKVLSYTILNDRLAGGYLLTGIRGV
GKTTSARIIAKAVNCSALITENTTIKTCEQCTNCISFNNHNHPDIIEIDAASKTSVDDIRRIIESAEYKPLQGKHKIFII
DEVHMLSKGAFNALLKTLEEPPPHVIFIFATTEVQKIPATIISRCQRYDLRRLSFEEIFKLLEYITKQENLKTDIEALRI
IAYKSEGSARDAVSILDQAASMSVKSDNIISPQVINQMLGLVDSSVIIEFVECIIYRETEKAINLINKLYGSSVNLEIFI
ESAADFIAYLNKVKMLPNYSLPIYESFNDRTKNILDKTSLPHLSILWQIYNKGVGEIKISYNQLTETEMLVIKSIYSTSL
PLLTDFDGNNQNLNQSINPEIKKKFEIVDFLEYLYKNNEIDIYYFLLNHTELKNLSNNRLEFVSLEVTSKIKRQIDDLLA
AFTNEKFEIVIIKEQNKQTLKNQLIGKIEASNDFGLIKKHFPNITISDILLKS

Sequences:

>Translated_533_residues
MSFPRKQEFSVKHIQNKFFSLITIVLDINSSNQYIPFARKYRPSNFAELQGQEVLVKVLSYTILNDRLAGGYLLTGIRGV
GKTTSARIIAKAVNCSALITENTTIKTCEQCTNCISFNNHNHPDIIEIDAASKTSVDDIRRIIESAEYKPLQGKHKIFII
DEVHMLSKGAFNALLKTLEEPPPHVIFIFATTEVQKIPATIISRCQRYDLRRLSFEEIFKLLEYITKQENLKTDIEALRI
IAYKSEGSARDAVSILDQAASMSVKSDNIISPQVINQMLGLVDSSVIIEFVECIIYRETEKAINLINKLYGSSVNLEIFI
ESAADFIAYLNKVKMLPNYSLPIYESFNDRTKNILDKTSLPHLSILWQIYNKGVGEIKISYNQLTETEMLVIKSIYSTSL
PLLTDFDGNNQNLNQSINPEIKKKFEIVDFLEYLYKNNEIDIYYFLLNHTELKNLSNNRLEFVSLEVTSKIKRQIDDLLA
AFTNEKFEIVIIKEQNKQTLKNQLIGKIEASNDFGLIKKHFPNITISDILLKS
>Mature_532_residues
SFPRKQEFSVKHIQNKFFSLITIVLDINSSNQYIPFARKYRPSNFAELQGQEVLVKVLSYTILNDRLAGGYLLTGIRGVG
KTTSARIIAKAVNCSALITENTTIKTCEQCTNCISFNNHNHPDIIEIDAASKTSVDDIRRIIESAEYKPLQGKHKIFIID
EVHMLSKGAFNALLKTLEEPPPHVIFIFATTEVQKIPATIISRCQRYDLRRLSFEEIFKLLEYITKQENLKTDIEALRII
AYKSEGSARDAVSILDQAASMSVKSDNIISPQVINQMLGLVDSSVIIEFVECIIYRETEKAINLINKLYGSSVNLEIFIE
SAADFIAYLNKVKMLPNYSLPIYESFNDRTKNILDKTSLPHLSILWQIYNKGVGEIKISYNQLTETEMLVIKSIYSTSLP
LLTDFDGNNQNLNQSINPEIKKKFEIVDFLEYLYKNNEIDIYYFLLNHTELKNLSNNRLEFVSLEVTSKIKRQIDDLLAA
FTNEKFEIVIIKEQNKQTLKNQLIGKIEASNDFGLIKKHFPNITISDILLKS

Specific function: The gamma chain seems to interact with the delta subunit to transfer the beta subunit on the DNA [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=303, Percent_Identity=39.9339933993399, Blast_Score=244, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71986063, Length=309, Percent_Identity=26.2135922330097, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17541988, Length=226, Percent_Identity=26.1061946902655, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324478, Length=299, Percent_Identity=25.0836120401338, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322528, Length=302, Percent_Identity=25.8278145695364, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17647857, Length=235, Percent_Identity=25.9574468085106, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18859927, Length=303, Percent_Identity=22.7722772277228, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR001270
- InterPro:   IPR008921
- InterPro:   IPR022001
- InterPro:   IPR022754
- InterPro:   IPR012763
- InterPro:   IPR021029 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3; PF12168 DNA_pol3_tau_4; PF12170 DNA_pol3_tau_5 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 60898; Mature: 60767

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPRKQEFSVKHIQNKFFSLITIVLDINSSNQYIPFARKYRPSNFAELQGQEVLVKVLS
CCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHCCCCCHHHHCHHHHHHHHHH
YTILNDRLAGGYLLTGIRGVGKTTSARIIAKAVNCSALITENTTIKTCEQCTNCISFNNH
HHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCC
NHPDIIEIDAASKTSVDDIRRIIESAEYKPLQGKHKIFIIDEVHMLSKGAFNALLKTLEE
CCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
PPPHVIFIFATTEVQKIPATIISRCQRYDLRRLSFEEIFKLLEYITKQENLKTDIEALRI
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHEEE
IAYKSEGSARDAVSILDQAASMSVKSDNIISPQVINQMLGLVDSSVIIEFVECIIYRETE
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAINLINKLYGSSVNLEIFIESAADFIAYLNKVKMLPNYSLPIYESFNDRTKNILDKTSL
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
PHLSILWQIYNKGVGEIKISYNQLTETEMLVIKSIYSTSLPLLTDFDGNNQNLNQSINPE
CHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
IKKKFEIVDFLEYLYKNNEIDIYYFLLNHTELKNLSNNRLEFVSLEVTSKIKRQIDDLLA
HHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHH
AFTNEKFEIVIIKEQNKQTLKNQLIGKIEASNDFGLIKKHFPNITISDILLKS
HHCCCCEEEEEEECCCHHHHHHHHHEEEECCCCCCHHHHHCCCCCHHHHEECC
>Mature Secondary Structure 
SFPRKQEFSVKHIQNKFFSLITIVLDINSSNQYIPFARKYRPSNFAELQGQEVLVKVLS
CCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHCCCCCHHHHCHHHHHHHHHH
YTILNDRLAGGYLLTGIRGVGKTTSARIIAKAVNCSALITENTTIKTCEQCTNCISFNNH
HHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCC
NHPDIIEIDAASKTSVDDIRRIIESAEYKPLQGKHKIFIIDEVHMLSKGAFNALLKTLEE
CCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
PPPHVIFIFATTEVQKIPATIISRCQRYDLRRLSFEEIFKLLEYITKQENLKTDIEALRI
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHEEE
IAYKSEGSARDAVSILDQAASMSVKSDNIISPQVINQMLGLVDSSVIIEFVECIIYRETE
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAINLINKLYGSSVNLEIFIESAADFIAYLNKVKMLPNYSLPIYESFNDRTKNILDKTSL
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCC
PHLSILWQIYNKGVGEIKISYNQLTETEMLVIKSIYSTSLPLLTDFDGNNQNLNQSINPE
CHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHH
IKKKFEIVDFLEYLYKNNEIDIYYFLLNHTELKNLSNNRLEFVSLEVTSKIKRQIDDLLA
HHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHH
AFTNEKFEIVIIKEQNKQTLKNQLIGKIEASNDFGLIKKHFPNITISDILLKS
HHCCCCEEEEEEECCCHHHHHHHHHEEEECCCCCCHHHHHCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534795; 3018672; 9278503; 2186364; 2187190; 2181440; 1575709 [H]