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The map label for this gene is gltX [H]

Identifier: 229586972

GI number: 229586972

Start: 915769

End: 917310

Strand: Direct

Name: gltX [H]

Synonym: RAF_ORF0869

Alternate gene names: 229586972

Gene position: 915769-917310 (Clockwise)

Preceding gene: 229586971

Following gene: 229586973

Centisome position: 71.63

GC content: 36.71

Gene sequence:

>1542_bases
ATGAATAATAACGTAATTACGAGATTTGCTCCGTCACCAACGGGGTTTTTACATATAGGTTCGGCAAGAACCGCCCTTTT
TAATTATTTGTTCGCAAGGCATCATAACGGTAAGTTCTTGCTTCGCATTGAAGACACTGATAAAGAAAGGTCAACTAAAG
AAGCGGTAGAAGCTATATTCTCAGGTCTAAAATGGCTAGGGCTTGATTGGAACGGTGAGGTTATATTCCAGTCTAAACGT
AATAATCTTTACAAAAAAGCTGCACTAAAATTACTGCAAAACGGTAAAGCATATTATTGTTTTACTCGCCAAGAAGAAAT
AGAAAGACAGCGACAACAAGCTTTAGAAAATAAACAGCATTTTATTTTTAATAGCGAGTGGCGTGACAAAGATCCATCTA
TCTATCCTACTGATATTAAGCCAGTAATACGTCTAAAAACTCCAAGAGAGGGTAGCATAACAATTCATGACACTTTACAA
GGTAAGGTAGTAATTGAAAACTCTCATATAGACGATATGGTACTATTAAGAGCAGACGGCACTGCTACTTACATGCTAGC
CGTTGTAGTAGACGATCATGATATGGGTATAACTCATATTATTAGAGGTGACGATCATTTAACCAATGCAGCAAGACAAC
TCGCTATTTATCAGGCTTTCGGATATGCAGTACCGAGCATGACTCATATACCGTTAATTCATGGAGCAGACGGAGCAAAA
TTATCCAAAAGGCATGGAGCATTAGGCATTGAAGCTTATAAGGATATGGGGTATTTACCGGAAAGTTTGTGCAATTATTT
ACTGCGTCTTGGATGGAGTCACGGCGATGATGAAATTATCTCAATGACTCAGGCTATAGACTGGTTTAATCTTGATTCAC
TCGGTAAATCACCTTCTAAGCTTGATTTTGCTAAGATGAATAGCCTTAACGCTCATTACTTAAGAATGCTTGACAATGAT
AGCTTAACTTCAAAGACTGTAGAGATATTGGAACAAAATTATAATACACTTCTTCGGAAGCTCCCTTATAGGGAGGAATT
TGGAGGAAACACGGAACGCAGCACCGCAGCGTATATAGACATACGTGAGGATGCGAGTACCGGATTGACGTACAAATTAC
CCCTAGCAGTAGAGTTGCCGAAGAAGTTTAAGATTAGTGAGCAGGAAATAGGCTATATCAAACAAGCAATGCCAAGCTTA
TTAGTTAGAAGTGAAACATTACTAGAACTAACACGCCTTGCTCAAATTTATCTAGTAGATTCGCCTATCATCTACAGCCA
AGATTCAAAAAAAATTATAGAAAATTGCGATAAGAATTTAATCAAACAAATTATAGAAAACTTAAGCAAACTTGAGCAGT
TTGATAAGGAATCCGTACAGAATAAATTTAAAGAAATAGCAGCAGCAAACGACTTAAAGCTGAATGACATTATGAAACCG
GTAAGAGCTTTAATAACAGGTATGACTGCATCACCTAGCGTATTTGAGATTGCAGAAATTTTAGGAAAAGAAAATATTTT
AAAAAGGTTAAAAATTATATGA

Upstream 100 bases:

>100_bases
TAAACATACTAATATTATTAGTATGTTTAGTTGTTTTCTGGATACCGTGGCAAGCCACGGTATGACACAGTTCTTAGTAT
ATATTTAAAAAAGATAATAA

Downstream 100 bases:

>100_bases
AAGATTGTATAAAAAAAATTGCTTTTATTTTTAGTGGATTATTCATAATAACCGGCACTTTTGTTTTGTATAGCATAGAA
AACATCAACGCATCAACAGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2 [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MNNNVITRFAPSPTGFLHIGSARTALFNYLFARHHNGKFLLRIEDTDKERSTKEAVEAIFSGLKWLGLDWNGEVIFQSKR
NNLYKKAALKLLQNGKAYYCFTRQEEIERQRQQALENKQHFIFNSEWRDKDPSIYPTDIKPVIRLKTPREGSITIHDTLQ
GKVVIENSHIDDMVLLRADGTATYMLAVVVDDHDMGITHIIRGDDHLTNAARQLAIYQAFGYAVPSMTHIPLIHGADGAK
LSKRHGALGIEAYKDMGYLPESLCNYLLRLGWSHGDDEIISMTQAIDWFNLDSLGKSPSKLDFAKMNSLNAHYLRMLDND
SLTSKTVEILEQNYNTLLRKLPYREEFGGNTERSTAAYIDIREDASTGLTYKLPLAVELPKKFKISEQEIGYIKQAMPSL
LVRSETLLELTRLAQIYLVDSPIIYSQDSKKIIENCDKNLIKQIIENLSKLEQFDKESVQNKFKEIAAANDLKLNDIMKP
VRALITGMTASPSVFEIAEILGKENILKRLKII

Sequences:

>Translated_513_residues
MNNNVITRFAPSPTGFLHIGSARTALFNYLFARHHNGKFLLRIEDTDKERSTKEAVEAIFSGLKWLGLDWNGEVIFQSKR
NNLYKKAALKLLQNGKAYYCFTRQEEIERQRQQALENKQHFIFNSEWRDKDPSIYPTDIKPVIRLKTPREGSITIHDTLQ
GKVVIENSHIDDMVLLRADGTATYMLAVVVDDHDMGITHIIRGDDHLTNAARQLAIYQAFGYAVPSMTHIPLIHGADGAK
LSKRHGALGIEAYKDMGYLPESLCNYLLRLGWSHGDDEIISMTQAIDWFNLDSLGKSPSKLDFAKMNSLNAHYLRMLDND
SLTSKTVEILEQNYNTLLRKLPYREEFGGNTERSTAAYIDIREDASTGLTYKLPLAVELPKKFKISEQEIGYIKQAMPSL
LVRSETLLELTRLAQIYLVDSPIIYSQDSKKIIENCDKNLIKQIIENLSKLEQFDKESVQNKFKEIAAANDLKLNDIMKP
VRALITGMTASPSVFEIAEILGKENILKRLKII
>Mature_513_residues
MNNNVITRFAPSPTGFLHIGSARTALFNYLFARHHNGKFLLRIEDTDKERSTKEAVEAIFSGLKWLGLDWNGEVIFQSKR
NNLYKKAALKLLQNGKAYYCFTRQEEIERQRQQALENKQHFIFNSEWRDKDPSIYPTDIKPVIRLKTPREGSITIHDTLQ
GKVVIENSHIDDMVLLRADGTATYMLAVVVDDHDMGITHIIRGDDHLTNAARQLAIYQAFGYAVPSMTHIPLIHGADGAK
LSKRHGALGIEAYKDMGYLPESLCNYLLRLGWSHGDDEIISMTQAIDWFNLDSLGKSPSKLDFAKMNSLNAHYLRMLDND
SLTSKTVEILEQNYNTLLRKLPYREEFGGNTERSTAAYIDIREDASTGLTYKLPLAVELPKKFKISEQEIGYIKQAMPSL
LVRSETLLELTRLAQIYLVDSPIIYSQDSKKIIENCDKNLIKQIIENLSKLEQFDKESVQNKFKEIAAANDLKLNDIMKP
VRALITGMTASPSVFEIAEILGKENILKRLKII

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RPE1 insert domain [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=354, Percent_Identity=35.0282485875706, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI4826960, Length=173, Percent_Identity=30.635838150289, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788743, Length=506, Percent_Identity=33.7944664031621, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=38.4615384615385, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786895, Length=129, Percent_Identity=32.5581395348837, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=532, Percent_Identity=29.1353383458647, Blast_Score=188, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6324540, Length=355, Percent_Identity=40.5633802816901, Blast_Score=240, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324742, Length=109, Percent_Identity=40.3669724770642, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=111, Percent_Identity=31.5315315315315, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=533, Percent_Identity=31.1444652908068, Blast_Score=231, Evalue=7e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR005728
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 58397; Mature: 58397

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNVITRFAPSPTGFLHIGSARTALFNYLFARHHNGKFLLRIEDTDKERSTKEAVEAIF
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHH
SGLKWLGLDWNGEVIFQSKRNNLYKKAALKLLQNGKAYYCFTRQEEIERQRQQALENKQH
CCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCC
FIFNSEWRDKDPSIYPTDIKPVIRLKTPREGSITIHDTLQGKVVIENSHIDDMVLLRADG
EEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEEEECC
TATYMLAVVVDDHDMGITHIIRGDDHLTNAARQLAIYQAFGYAVPSMTHIPLIHGADGAK
CEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
LSKRHGALGIEAYKDMGYLPESLCNYLLRLGWSHGDDEIISMTQAIDWFNLDSLGKSPSK
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHCCCCCCC
LDFAKMNSLNAHYLRMLDNDSLTSKTVEILEQNYNTLLRKLPYREEFGGNTERSTAAYID
CCHHHHCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEE
IREDASTGLTYKLPLAVELPKKFKISEQEIGYIKQAMPSLLVRSETLLELTRLAQIYLVD
EECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
SPIIYSQDSKKIIENCDKNLIKQIIENLSKLEQFDKESVQNKFKEIAAANDLKLNDIMKP
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VRALITGMTASPSVFEIAEILGKENILKRLKII
HHHHHHCCCCCCHHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure
MNNNVITRFAPSPTGFLHIGSARTALFNYLFARHHNGKFLLRIEDTDKERSTKEAVEAIF
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHH
SGLKWLGLDWNGEVIFQSKRNNLYKKAALKLLQNGKAYYCFTRQEEIERQRQQALENKQH
CCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCC
FIFNSEWRDKDPSIYPTDIKPVIRLKTPREGSITIHDTLQGKVVIENSHIDDMVLLRADG
EEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEEEECC
TATYMLAVVVDDHDMGITHIIRGDDHLTNAARQLAIYQAFGYAVPSMTHIPLIHGADGAK
CEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
LSKRHGALGIEAYKDMGYLPESLCNYLLRLGWSHGDDEIISMTQAIDWFNLDSLGKSPSK
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHCCCCCCC
LDFAKMNSLNAHYLRMLDNDSLTSKTVEILEQNYNTLLRKLPYREEFGGNTERSTAAYID
CCHHHHCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEEEE
IREDASTGLTYKLPLAVELPKKFKISEQEIGYIKQAMPSLLVRSETLLELTRLAQIYLVD
EECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
SPIIYSQDSKKIIENCDKNLIKQIIENLSKLEQFDKESVQNKFKEIAAANDLKLNDIMKP
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VRALITGMTASPSVFEIAEILGKENILKRLKII
HHHHHHCCCCCCHHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]