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The map label for this gene is pheT [H]

Identifier: 229586696

GI number: 229586696

Start: 569684

End: 572140

Strand: Direct

Name: pheT [H]

Synonym: RAF_ORF0543

Alternate gene names: 229586696

Gene position: 569684-572140 (Clockwise)

Preceding gene: 229586695

Following gene: 229586697

Centisome position: 44.56

GC content: 34.31

Gene sequence:

>2457_bases
ATGAAATTTACGTTATCATGGTTAAAACAATTTTTAGAGACATCGGCTTCAGTTACTGAAATTGCCGAAGCCCTAACAGC
TATTGGCCTTGAAGTGGAAGAGGTGATAGATAAAGCGGCGGAATTACAAAAGTTTGAAGTAGCATATATTACAAATATTA
AACCTCACCCGTCAGCTGATAAATTAAAGCTTTGTGATGTTGAGACTAAAAGCGGTATGCTGCAAATAGTTTGTGGTGCG
CGTAATGTAAGAGCAGGTATAAAAGTAGTACTTGCAAATATCGGAATAGAAATACCAAACGGTAAATTTAAGATTAAGGA
ATCTGTTATTAGAGGCGAAAAAAGCTGTGGTATGCTTTGCTCTGAAGAGGAATTACTGCTAGCTTCAGAGTCTGAAGGGA
TTATAGAGCTATCTGAAGATGCCGTAGTTGGAGAGAATTTTACTAAATATTACGGTTTAGATGATCCTATATTTGTTATT
AACGTTACTCCTAATCGTGGTGATGCACTTGGAGTTTACGGCATCGCAAGAGATTTAGCAGCTAAAGGAATAGGAATACT
TAAAGAGCTAGAAATTTCGCCAATAAAGAGTACATTTATTTCTAAAATGAAGCTTAACGTGCAAGATAAGGAAGCCTGTC
CGTTATTTACTTTTAGAGAGATAAGAAATTTAAAAAATAAACCAAGCCCTGATTGGTTACGGAAATTATTAAAAAATGTT
GGGGTAAAAACTATTTCAAGCTTAGTGGATGTAACAAATTATATTTCCTATAGCTTTGGGCAGCCGATGCATGCTTACGA
TGCAGATAGGATAAAAGGGGGAATTACTGTAGCACGTCATTGCGAGAAGCGTAGCGACGAAGCAATCTCAGGACAACAAA
ATGAGATTGCCACGGCTGCTTTGCAGCCTCGCAATGACGTAGCTAAATGCCACGCCCTAAACGGCAAAGAATATTTACTT
ACAGAAAATGATTTAGTTATAAAAGATGAAAGCGGTATTCAAGGTCTGGCCGGTGTCATTGGCGAGGCTGATAGTAGTTG
TACTGATAGTACGACTAATATAATACTCGAGGCTGCTTGTTTTAATGCTAAAATGGTTGCAGCTAGCGGGCGAAGGTTCC
AAATTGATACGGATGCTAGATATCGTAATGAGCGTAATATCGATAGAAATTTTACGGAAAAGGCTTTGGATATAGCAACT
AATCTTATTTTGTCAATATGCGGAAACGGTGAAGTATCGGAAGTAGTGAAAGTCGGTGAAAAAGAGTCACAAAAAAAACC
TTTAGATTTTTCAGCATGTTATTTAGAAAAAATTACAGGAATTAAACTAAATATCAAAGCGATAGAAGCCATATTAAATA
AACTAGGATTTATTACGGATGTTAAAGGTGAGCTTATAAAGGTAATAGCTCCTTCTTGGCGTCACGATATAACTATTTTA
GAAGATATAGCTGAAGAAATCGCTCGTATTTACGGCTATGACAAAATAGAGAGTATAAAATTACCTGAACTAGACCAAGA
TAATAATAAGCTAAGAGAGCATAAAAGAATATCTAGTTTTAAAAGGATATTAGCAAGCAAAGGCTATGATGAGGTAGTAA
CTAACTCTTTTATGAGTAGTGAAGATGCAAAGTTGTTTGCTGAATTAAAAGAAGAGTTATTTTTGCTTAATCCTATTAGC
ATAGGAGACAATTATATGCGTCCTACTATACTGCCGAATTTGTTAAGTATAGTAAGTAAAAATCTAGCACGCTCTATAAA
AGATATGGCTTTTTTTGAAGTTGGACCAAGTTTTATAGATTTAAATACGGAAGCAACTTATTTGACGGCAATTATAAGCG
GTTCATATAATAATAAAAACCCTCATTCGCTTGGACGAGGCTATGATGTTTTTGATCTTAAAGGTGATTTAGAGTTGGTA
GCTGATTATGCAGGACTATCTATAGATAAATGTATAGCAACAAATGGGACAGCATTGCCACAATATTATCATCCAACTAG
AGCAGTAAATATAGGGCTTGGTAAAAATTTATTAGGTCATTTTGGGCAGATACATCCTAAAATCTTGAAATATTATGATA
TTAATCAGGAAATATTTGCATTCGAGCTAAATATTACGAATTTGCCGTTGATAAAAGCTAAATTTGGTAAAAGAGACGAA
TTTGCAGTATCGGATTTCCAAGCTAATTTTAGAGATTACGCATTTATTGTTGGTCAAGATCATAGAGTCGGTGAAATAAT
ATCATATATAAATAATTTTAATACAAAGCTTATCAAATCAGTTATATTATTTGATATTTATAGTGGTGATAAATTACCTG
AAGGTAAAAAATCTATAGCGGTTAAAATAGAACTACAAGCAGATGATCGGACGTTATCTGATACTGATTTAAATTCGTTC
AGTAAAGATTTAGTTGCTGCCATCTCACAAAAATTTCAAGGAACATTAAGAGAATAA

Upstream 100 bases:

>100_bases
TCAAAGATTTAAGACAATTTTTTGAAGGCGATATGCGTTGGCTTAAACATTATAATTTTGGGAGCTTTGATATACCGAAT
TTAGCAGGAGGGCTTACGAA

Downstream 100 bases:

>100_bases
CAATATGTTAAAATTAATAGTTGAAACAAAAACATTAGTTCAGTCCCTAGGGTTTGCAAGTTCCGTTGTTGAAAAACGTA
ATGTCATACCTGAATATGCA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 818; Mature: 818

Protein sequence:

>818_residues
MKFTLSWLKQFLETSASVTEIAEALTAIGLEVEEVIDKAAELQKFEVAYITNIKPHPSADKLKLCDVETKSGMLQIVCGA
RNVRAGIKVVLANIGIEIPNGKFKIKESVIRGEKSCGMLCSEEELLLASESEGIIELSEDAVVGENFTKYYGLDDPIFVI
NVTPNRGDALGVYGIARDLAAKGIGILKELEISPIKSTFISKMKLNVQDKEACPLFTFREIRNLKNKPSPDWLRKLLKNV
GVKTISSLVDVTNYISYSFGQPMHAYDADRIKGGITVARHCEKRSDEAISGQQNEIATAALQPRNDVAKCHALNGKEYLL
TENDLVIKDESGIQGLAGVIGEADSSCTDSTTNIILEAACFNAKMVAASGRRFQIDTDARYRNERNIDRNFTEKALDIAT
NLILSICGNGEVSEVVKVGEKESQKKPLDFSACYLEKITGIKLNIKAIEAILNKLGFITDVKGELIKVIAPSWRHDITIL
EDIAEEIARIYGYDKIESIKLPELDQDNNKLREHKRISSFKRILASKGYDEVVTNSFMSSEDAKLFAELKEELFLLNPIS
IGDNYMRPTILPNLLSIVSKNLARSIKDMAFFEVGPSFIDLNTEATYLTAIISGSYNNKNPHSLGRGYDVFDLKGDLELV
ADYAGLSIDKCIATNGTALPQYYHPTRAVNIGLGKNLLGHFGQIHPKILKYYDINQEIFAFELNITNLPLIKAKFGKRDE
FAVSDFQANFRDYAFIVGQDHRVGEIISYINNFNTKLIKSVILFDIYSGDKLPEGKKSIAVKIELQADDRTLSDTDLNSF
SKDLVAAISQKFQGTLRE

Sequences:

>Translated_818_residues
MKFTLSWLKQFLETSASVTEIAEALTAIGLEVEEVIDKAAELQKFEVAYITNIKPHPSADKLKLCDVETKSGMLQIVCGA
RNVRAGIKVVLANIGIEIPNGKFKIKESVIRGEKSCGMLCSEEELLLASESEGIIELSEDAVVGENFTKYYGLDDPIFVI
NVTPNRGDALGVYGIARDLAAKGIGILKELEISPIKSTFISKMKLNVQDKEACPLFTFREIRNLKNKPSPDWLRKLLKNV
GVKTISSLVDVTNYISYSFGQPMHAYDADRIKGGITVARHCEKRSDEAISGQQNEIATAALQPRNDVAKCHALNGKEYLL
TENDLVIKDESGIQGLAGVIGEADSSCTDSTTNIILEAACFNAKMVAASGRRFQIDTDARYRNERNIDRNFTEKALDIAT
NLILSICGNGEVSEVVKVGEKESQKKPLDFSACYLEKITGIKLNIKAIEAILNKLGFITDVKGELIKVIAPSWRHDITIL
EDIAEEIARIYGYDKIESIKLPELDQDNNKLREHKRISSFKRILASKGYDEVVTNSFMSSEDAKLFAELKEELFLLNPIS
IGDNYMRPTILPNLLSIVSKNLARSIKDMAFFEVGPSFIDLNTEATYLTAIISGSYNNKNPHSLGRGYDVFDLKGDLELV
ADYAGLSIDKCIATNGTALPQYYHPTRAVNIGLGKNLLGHFGQIHPKILKYYDINQEIFAFELNITNLPLIKAKFGKRDE
FAVSDFQANFRDYAFIVGQDHRVGEIISYINNFNTKLIKSVILFDIYSGDKLPEGKKSIAVKIELQADDRTLSDTDLNSF
SKDLVAAISQKFQGTLRE
>Mature_818_residues
MKFTLSWLKQFLETSASVTEIAEALTAIGLEVEEVIDKAAELQKFEVAYITNIKPHPSADKLKLCDVETKSGMLQIVCGA
RNVRAGIKVVLANIGIEIPNGKFKIKESVIRGEKSCGMLCSEEELLLASESEGIIELSEDAVVGENFTKYYGLDDPIFVI
NVTPNRGDALGVYGIARDLAAKGIGILKELEISPIKSTFISKMKLNVQDKEACPLFTFREIRNLKNKPSPDWLRKLLKNV
GVKTISSLVDVTNYISYSFGQPMHAYDADRIKGGITVARHCEKRSDEAISGQQNEIATAALQPRNDVAKCHALNGKEYLL
TENDLVIKDESGIQGLAGVIGEADSSCTDSTTNIILEAACFNAKMVAASGRRFQIDTDARYRNERNIDRNFTEKALDIAT
NLILSICGNGEVSEVVKVGEKESQKKPLDFSACYLEKITGIKLNIKAIEAILNKLGFITDVKGELIKVIAPSWRHDITIL
EDIAEEIARIYGYDKIESIKLPELDQDNNKLREHKRISSFKRILASKGYDEVVTNSFMSSEDAKLFAELKEELFLLNPIS
IGDNYMRPTILPNLLSIVSKNLARSIKDMAFFEVGPSFIDLNTEATYLTAIISGSYNNKNPHSLGRGYDVFDLKGDLELV
ADYAGLSIDKCIATNGTALPQYYHPTRAVNIGLGKNLLGHFGQIHPKILKYYDINQEIFAFELNITNLPLIKAKFGKRDE
FAVSDFQANFRDYAFIVGQDHRVGEIISYINNFNTKLIKSVILFDIYSGDKLPEGKKSIAVKIELQADDRTLSDTDLNSF
SKDLVAAISQKFQGTLRE

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=835, Percent_Identity=33.6526946107784, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17533403, Length=518, Percent_Identity=22.3938223938224, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 90820; Mature: 90820

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKQFLETSASVTEIAEALTAIGLEVEEVIDKAAELQKFEVAYITNIKPHPSAD
CCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCC
KLKLCDVETKSGMLQIVCGARNVRAGIKVVLANIGIEIPNGKFKIKESVIRGEKSCGMLC
CEEEECCCCCCCEEEEEECCCCHHHHHHEEEEECCEECCCCCEEEHHHHHCCCHHCCCCC
SEEELLLASESEGIIELSEDAVVGENFTKYYGLDDPIFVINVTPNRGDALGVYGIARDLA
CCCCEEEECCCCCCEEECCCCEECCCCHHHCCCCCCEEEEEECCCCCCEEEHHHHHHHHH
AKGIGILKELEISPIKSTFISKMKLNVQDKEACPLFTFREIRNLKNKPSPDWLRKLLKNV
HCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
GVKTISSLVDVTNYISYSFGQPMHAYDADRIKGGITVARHCEKRSDEAISGQQNEIATAA
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHCCCHHCCCCCCHHHHEE
LQPRNDVAKCHALNGKEYLLTENDLVIKDESGIQGLAGVIGEADSSCTDSTTNIILEAAC
CCCCCHHHHHEECCCCEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHEEEEEHH
FNAKMVAASGRRFQIDTDARYRNERNIDRNFTEKALDIATNLILSICGNGEVSEVVKVGE
CCCEEEECCCCEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
KESQKKPLDFSACYLEKITGIKLNIKAIEAILNKLGFITDVKGELIKVIAPSWRHDITIL
CHHCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHCCEECCCHHHHHHCCCCCCCCHHHH
EDIAEEIARIYGYDKIESIKLPELDQDNNKLREHKRISSFKRILASKGYDEVVTNSFMSS
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
EDAKLFAELKEELFLLNPISIGDNYMRPTILPNLLSIVSKNLARSIKDMAFFEVGPSFID
HHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LNTEATYLTAIISGSYNNKNPHSLGRGYDVFDLKGDLELVADYAGLSIDKCIATNGTALP
CCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHCCCCCCCC
QYYHPTRAVNIGLGKNLLGHFGQIHPKILKYYDINQEIFAFELNITNLPLIKAKFGKRDE
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHEECCCEEEEEEEECCCCCEEECCCCCCCC
FAVSDFQANFRDYAFIVGQDHRVGEIISYINNFNTKLIKSVILFDIYSGDKLPEGKKSIA
CCHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCCCEEE
VKIELQADDRTLSDTDLNSFSKDLVAAISQKFQGTLRE
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFTLSWLKQFLETSASVTEIAEALTAIGLEVEEVIDKAAELQKFEVAYITNIKPHPSAD
CCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCC
KLKLCDVETKSGMLQIVCGARNVRAGIKVVLANIGIEIPNGKFKIKESVIRGEKSCGMLC
CEEEECCCCCCCEEEEEECCCCHHHHHHEEEEECCEECCCCCEEEHHHHHCCCHHCCCCC
SEEELLLASESEGIIELSEDAVVGENFTKYYGLDDPIFVINVTPNRGDALGVYGIARDLA
CCCCEEEECCCCCCEEECCCCEECCCCHHHCCCCCCEEEEEECCCCCCEEEHHHHHHHHH
AKGIGILKELEISPIKSTFISKMKLNVQDKEACPLFTFREIRNLKNKPSPDWLRKLLKNV
HCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHC
GVKTISSLVDVTNYISYSFGQPMHAYDADRIKGGITVARHCEKRSDEAISGQQNEIATAA
CHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHCCCHHCCCCCCHHHHEE
LQPRNDVAKCHALNGKEYLLTENDLVIKDESGIQGLAGVIGEADSSCTDSTTNIILEAAC
CCCCCHHHHHEECCCCEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHEEEEEHH
FNAKMVAASGRRFQIDTDARYRNERNIDRNFTEKALDIATNLILSICGNGEVSEVVKVGE
CCCEEEECCCCEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
KESQKKPLDFSACYLEKITGIKLNIKAIEAILNKLGFITDVKGELIKVIAPSWRHDITIL
CHHCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHCCEECCCHHHHHHCCCCCCCCHHHH
EDIAEEIARIYGYDKIESIKLPELDQDNNKLREHKRISSFKRILASKGYDEVVTNSFMSS
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
EDAKLFAELKEELFLLNPISIGDNYMRPTILPNLLSIVSKNLARSIKDMAFFEVGPSFID
HHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LNTEATYLTAIISGSYNNKNPHSLGRGYDVFDLKGDLELVADYAGLSIDKCIATNGTALP
CCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHCCCCCCCC
QYYHPTRAVNIGLGKNLLGHFGQIHPKILKYYDINQEIFAFELNITNLPLIKAKFGKRDE
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHEECCCEEEEEEEECCCCCEEECCCCCCCC
FAVSDFQANFRDYAFIVGQDHRVGEIISYINNFNTKLIKSVILFDIYSGDKLPEGKKSIA
CCHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCCCEEE
VKIELQADDRTLSDTDLNSFSKDLVAAISQKFQGTLRE
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]