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The map label for this gene is dnaN [H]

Identifier: 229586697

GI number: 229586697

Start: 572145

End: 573284

Strand: Direct

Name: dnaN [H]

Synonym: RAF_ORF0544

Alternate gene names: 229586697

Gene position: 572145-573284 (Clockwise)

Preceding gene: 229586696

Following gene: 229586698

Centisome position: 44.75

GC content: 31.75

Gene sequence:

>1140_bases
ATGTTAAAATTAATAGTTGAAACAAAAACATTAGTTCAGTCCCTAGGGTTTGCAAGTTCCGTTGTTGAAAAACGTAATGT
CATACCTGAATATGCAAACATTAAATTATTGGCAAAAGACGGCAATCTAGAGCTTAGTTCTACTAATATGGATTTGTACT
TAAGTCAAAAAATAGCAGTACAGGTAGTAAGTGAAGGTGAATGTACCGTTTCTACGAAAACTCTAAATGATATAGTCCGA
AAACTTCCGGATTCCGAGCTTACATTAACAGATCTCGGTACAACGGGACTTGAGATCAAAGGAAAAAATTGTAAATTTAA
TTTATTTACTTTGCCGGTTAGTTCTTTTCCTGCAATGGATAGTATTAATCCTGAAGCAAGTTTTAAAATTTCCTGCACGG
ATTTTGCTAAAATAATCGAATCGACAAAATTTTCGATTTCTTTAGATGAAACTCGTTATAATTTAAACGGTGTTTATTTA
CACATAAAAGACAAAGAGTTTTGTTCGGCAAGTACCGATGGGCATAGACTTTCGATTTCATGGGTAACATTAGAAAAACA
AATAAAGAATTTTGGAGTTATATTACCCCAAAAAAGTGCAGAGGAAATTTTAAAAATCGTTAAAGATCCTAAAAATATAA
ATGAAGATATAGAAATTTTATTAAGTAGCAATAAGATTAAATTTATATGTAACGAAAATACTAGTATGCTATCAAAGCTT
ATAGACGGTACTTTCCCTGATTATAGTACTTTTATTCCGGAAAGCAGCAGCTCAAAATTAGTAATTAATCGAAAAATGTT
TGCAGATAGTATAGAAAGGATAGCGATAATAACCGTTGAAAAATTTAGAGCAGTAAAATTATCGTTATCTCGTGAAACCT
TAGAGATTAGTGCCGTAGGTGAAGCAAGAGGTAACGCAAAAGAGGTTATTAATTCTTCACAAGATAAGGAAAGTTTTTAT
GAATATAATAGTGATGAGTCTTTAGCTATAGGCTTTAACCCGCAATATTTGGAAGATGTCTTAAAAGCAGTGAAATCGGA
TGTAGTAGAACTATATTTTTCAGATGTTTCAGCACCGGTACTTATTAAATTTCCTGAGAATCCTAAAGATATTTTTGTCG
TCATGCCTGTTAAGGTGTAA

Upstream 100 bases:

>100_bases
GATGATCGGACGTTATCTGATACTGATTTAAATTCGTTCAGTAAAGATTTAGTTGCTGCCATCTCACAAAAATTTCAAGG
AACATTAAGAGAATAACAAT

Downstream 100 bases:

>100_bases
GTGTTATGAGAACCGGAATATATAAGCATTACAAAGAAAAATTATATCAAGTTATAGATACTGCAAAACATACTGAAACT
TTGCAAGAGTTATATGATAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLLAKDGNLELSSTNMDLYLSQKIAVQVVSEGECTVSTKTLNDIVR
KLPDSELTLTDLGTTGLEIKGKNCKFNLFTLPVSSFPAMDSINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYL
HIKDKEFCSASTDGHRLSISWVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEILLSSNKIKFICNENTSMLSKL
IDGTFPDYSTFIPESSSSKLVINRKMFADSIERIAIITVEKFRAVKLSLSRETLEISAVGEARGNAKEVINSSQDKESFY
EYNSDESLAIGFNPQYLEDVLKAVKSDVVELYFSDVSAPVLIKFPENPKDIFVVMPVKV

Sequences:

>Translated_379_residues
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLLAKDGNLELSSTNMDLYLSQKIAVQVVSEGECTVSTKTLNDIVR
KLPDSELTLTDLGTTGLEIKGKNCKFNLFTLPVSSFPAMDSINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYL
HIKDKEFCSASTDGHRLSISWVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEILLSSNKIKFICNENTSMLSKL
IDGTFPDYSTFIPESSSSKLVINRKMFADSIERIAIITVEKFRAVKLSLSRETLEISAVGEARGNAKEVINSSQDKESFY
EYNSDESLAIGFNPQYLEDVLKAVKSDVVELYFSDVSAPVLIKFPENPKDIFVVMPVKV
>Mature_379_residues
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLLAKDGNLELSSTNMDLYLSQKIAVQVVSEGECTVSTKTLNDIVR
KLPDSELTLTDLGTTGLEIKGKNCKFNLFTLPVSSFPAMDSINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYL
HIKDKEFCSASTDGHRLSISWVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEILLSSNKIKFICNENTSMLSKL
IDGTFPDYSTFIPESSSSKLVINRKMFADSIERIAIITVEKFRAVKLSLSRETLEISAVGEARGNAKEVINSSQDKESFY
EYNSDESLAIGFNPQYLEDVLKAVKSDVVELYFSDVSAPVLIKFPENPKDIFVVMPVKV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=379, Percent_Identity=28.7598944591029, Blast_Score=181, Evalue=5e-47,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42263; Mature: 42263

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLLAKDGNLELSSTNMDLYLSQKIAV
CEEEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEECCCEEEEEECEEEE
QVVSEGECTVSTKTLNDIVRKLPDSELTLTDLGTTGLEIKGKNCKFNLFTLPVSSFPAMD
EEECCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCEEEEEEEECCCCCCCCC
SINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYLHIKDKEFCSASTDGHRLSIS
CCCCCCEEEEEHHHHHHHHHCCCEEEEEECCEEECCEEEEEEECHHHCCCCCCCCEEEEE
WVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEILLSSNKIKFICNENTSMLSKL
EEEEHHHHHHCCEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHH
IDGTFPDYSTFIPESSSSKLVINRKMFADSIERIAIITVEKFRAVKLSLSRETLEISAVG
HCCCCCCCHHCCCCCCCCEEEEEHHHHHHCCCEEEEEEEECEEEEEEEECCCEEEEEEEC
EARGNAKEVINSSQDKESFYEYNSDESLAIGFNPQYLEDVLKAVKSDVVELYFSDVSAPV
CCCCCHHHHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LIKFPENPKDIFVVMPVKV
EEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLLAKDGNLELSSTNMDLYLSQKIAV
CEEEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEECCCEEEEEECEEEE
QVVSEGECTVSTKTLNDIVRKLPDSELTLTDLGTTGLEIKGKNCKFNLFTLPVSSFPAMD
EEECCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCEEEEEEEECCCCCCCCC
SINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYLHIKDKEFCSASTDGHRLSIS
CCCCCCEEEEEHHHHHHHHHCCCEEEEEECCEEECCEEEEEEECHHHCCCCCCCCEEEEE
WVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEILLSSNKIKFICNENTSMLSKL
EEEEHHHHHHCCEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCHHHHHHH
IDGTFPDYSTFIPESSSSKLVINRKMFADSIERIAIITVEKFRAVKLSLSRETLEISAVG
HCCCCCCCHHCCCCCCCCEEEEEHHHHHHCCCEEEEEEEECEEEEEEEECCCEEEEEEEC
EARGNAKEVINSSQDKESFYEYNSDESLAIGFNPQYLEDVLKAVKSDVVELYFSDVSAPV
CCCCCHHHHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LIKFPENPKDIFVVMPVKV
EEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]