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The map label for this gene is pheS

Identifier: 229586695

GI number: 229586695

Start: 568638

End: 569687

Strand: Direct

Name: pheS

Synonym: RAF_ORF0542

Alternate gene names: 229586695

Gene position: 568638-569687 (Clockwise)

Preceding gene: 229586694

Following gene: 229586696

Centisome position: 44.48

GC content: 33.9

Gene sequence:

>1050_bases
ATGGAAAATATAGAAACAATATTAAGGCTTGCTGAGGACAAAATCTTATTAGTACAAAACTTAAAAGCTCTGCAAGAATA
TAAAGTAGAATTTTTAGGCAAGAACGGTATAGTGACCGGTGAGCTTAAAAAATTAGGTAGTTTAAATGAACAGGAACGTA
AAGAATTTGGCTTAAAAATCAATAAATTAAAAGATAAAATACAGAATATAATAAAAGCAAAAGCAGAAATTTTAGAGGAG
CAGGAACTAAATTTTAAACTTGCTGCCGATAAAATTGATTTAACAATCCCTGCACGGAGATATAAACAAGGTTCTATTCA
TCCAATAACACAATGTAGTGAGGAGTTAATACAAGTATTTTCGCAGTTTGGTTTTACTATAGAAAACGGACCGAATATCG
AGAATGATTTTTATAATTTTACCGCTCTCAATTTTGAAGATGATCATCCCGCAAGGCAAATGCATGATACTTTTTATTTG
AAAAGTCAGGAAAATAATAAGCCGCTGTTATTACGTACTCATACCTCAACAGTGCAGATTAGAGCTATGAAAAACGGCAA
ACCGCCTTTTAGGTTTATAGCACCGGGTAGGACTTATAGATCGGATTCGGATATGACGCATACGCCAATGTTCCACCAAA
TAGAAGGGCTTGTTATGGATAAAAATATCAATATGGGACATTTAAAATATGTTATCACGACATTTATAAAAAGCTTTTTT
GAAAATTCTAATATTGAATTACGTTTTAGACCCAGCTTTTTCCCATTTACCGAACCTTCTTCCGAAGTTGATATTCGGAT
GAATAAAAACGATAAATGGCTTGAGGTCCTCGGTTGCGGGATGGTTCATCCAAACGTGCTTAAAAATGTTGGTATCGATA
GCAGCGAGTATCAAGGTTTTGCTTTTGGGCTTGGAGTAGAGCGTTTTGCAATGTTAAAATATAATATCAAAGATTTAAGA
CAATTTTTTGAAGGCGATATGCGTTGGCTTAAACATTATAATTTTGGGAGCTTTGATATACCGAATTTAGCAGGAGGGCT
TACGAAATGA

Upstream 100 bases:

>100_bases
GCGATTTCCTTGCAATGACGGATAAACAGGTCCACGCAACAATGCCCGCTCGCAATGACGTTCTACCTTACAATAAAAAA
TCAAAAACAAAAAAACAATC

Downstream 100 bases:

>100_bases
AATTTACGTTATCATGGTTAAAACAATTTTTAGAGACATCGGCTTCAGTTACTGAAATTGCCGAAGCCCTAACAGCTATT
GGCCTTGAAGTGGAAGAGGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MENIETILRLAEDKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKINKLKDKIQNIIKAKAEILEE
QELNFKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVFSQFGFTIENGPNIENDFYNFTALNFEDDHPARQMHDTFYL
KSQENNKPLLLRTHTSTVQIRAMKNGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVMDKNINMGHLKYVITTFIKSFF
ENSNIELRFRPSFFPFTEPSSEVDIRMNKNDKWLEVLGCGMVHPNVLKNVGIDSSEYQGFAFGLGVERFAMLKYNIKDLR
QFFEGDMRWLKHYNFGSFDIPNLAGGLTK

Sequences:

>Translated_349_residues
MENIETILRLAEDKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKINKLKDKIQNIIKAKAEILEE
QELNFKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVFSQFGFTIENGPNIENDFYNFTALNFEDDHPARQMHDTFYL
KSQENNKPLLLRTHTSTVQIRAMKNGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVMDKNINMGHLKYVITTFIKSFF
ENSNIELRFRPSFFPFTEPSSEVDIRMNKNDKWLEVLGCGMVHPNVLKNVGIDSSEYQGFAFGLGVERFAMLKYNIKDLR
QFFEGDMRWLKHYNFGSFDIPNLAGGLTK
>Mature_349_residues
MENIETILRLAEDKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKINKLKDKIQNIIKAKAEILEE
QELNFKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVFSQFGFTIENGPNIENDFYNFTALNFEDDHPARQMHDTFYL
KSQENNKPLLLRTHTSTVQIRAMKNGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVMDKNINMGHLKYVITTFIKSFF
ENSNIELRFRPSFFPFTEPSSEVDIRMNKNDKWLEVLGCGMVHPNVLKNVGIDSSEYQGFAFGLGVERFAMLKYNIKDLR
QFFEGDMRWLKHYNFGSFDIPNLAGGLTK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=261, Percent_Identity=32.9501915708812, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI5729820, Length=256, Percent_Identity=30.859375, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1788007, Length=333, Percent_Identity=49.5495495495495, Blast_Score=352, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=29.6153846153846, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=29.6153846153846, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=286, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=261, Percent_Identity=31.0344827586207, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=277, Percent_Identity=27.4368231046931, Blast_Score=92, Evalue=8e-20,
Organism=Drosophila melanogaster, GI18858079, Length=262, Percent_Identity=29.0076335877863, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=30.3149606299213, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_RICAE (C3PNE3)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845196.1
- GeneID:   7814959
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0542
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40435; Mature: 40435

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIETILRLAEDKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHCCCH
NKLKDKIQNIIKAKAEILEEQELNFKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECHHHCCCCCCCHHHHHHHHHHHHH
SQFGFTIENGPNIENDFYNFTALNFEDDHPARQMHDTFYLKSQENNKPLLLRTHTSTVQI
HHCCCEECCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEEEECCCCCCEEEEECCCEEEE
RAMKNGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVMDKNINMGHLKYVITTFIKSFF
EEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHH
ENSNIELRFRPSFFPFTEPSSEVDIRMNKNDKWLEVLGCGMVHPNVLKNVGIDSSEYQGF
CCCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEHHHHCCCCCHHHHHHCCCCCCCCCCE
AFGLGVERFAMLKYNIKDLRQFFEGDMRWLKHYNFGSFDIPNLAGGLTK
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MENIETILRLAEDKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHCCCH
NKLKDKIQNIIKAKAEILEEQELNFKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECHHHCCCCCCCHHHHHHHHHHHHH
SQFGFTIENGPNIENDFYNFTALNFEDDHPARQMHDTFYLKSQENNKPLLLRTHTSTVQI
HHCCCEECCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEEEECCCCCCEEEEECCCEEEE
RAMKNGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVMDKNINMGHLKYVITTFIKSFF
EEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHH
ENSNIELRFRPSFFPFTEPSSEVDIRMNKNDKWLEVLGCGMVHPNVLKNVGIDSSEYQGF
CCCCEEEEECCCCCCCCCCCCCEEEEECCCCCEEHHHHCCCCCHHHHHHCCCCCCCCCCE
AFGLGVERFAMLKYNIKDLRQFFEGDMRWLKHYNFGSFDIPNLAGGLTK
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA