Click here to switch to the map view.

The map label for this gene is dut

Identifier: 229586671

GI number: 229586671

Start: 537090

End: 537536

Strand: Direct

Name: dut

Synonym: RAF_ORF0508

Alternate gene names: 229586671

Gene position: 537090-537536 (Clockwise)

Preceding gene: 229586670

Following gene: 229586672

Centisome position: 42.01

GC content: 40.72

Gene sequence:

>447_bases
ATGACAATAACACAAGTTAAAATTAAAAAACTAGAAAATTTTTCCGGCAGTTTGCCGGAATATGCTACAGAGCATAGTGC
GGGGATGGATTTAATAGCCGCAAACGAGCAACCTATAACAATAAAAGCAGCCGCGATACAGCTAATTCCAACAGGCATCG
CTATAGCACTACCGGATTCGTTTGAAGCCCAAATAAGACCCCGTTCGGGTCTTGCCGTTAAACACGGTATCACGGTTGCT
AATTCACCCGGTACTATTGATGCAGATTATCGAGGTGAAATAAAGGTCATTCTTATTAATCTTGGTAAAGAAGATTTTAT
TATAGAAAAAGGCATGCGAATTGCACAGATGATAATCGCAAAGTACGAACGTATATTATGGGAAGAAAGTAGTAGTCTTA
TGGAAACGATGCGTGGTAGCGGTGGCTTTGGTTCTACCGGTGTTTAG

Upstream 100 bases:

>100_bases
CTTGGCATGGGAAGTAAGATATTAACCGATAATCTTGCTAATTCTTTAATTGATGCGGTAGAAAATAAAATTATTAACGA
TAAATTTGATATGAAATAAT

Downstream 100 bases:

>100_bases
TGGGTCTCTATGTCATTCCCGCCGTAGGCGGGAATCCAGAAAAATAACCTTAATATTGATACAAAAATTACATATTAGAT
AAACCTATAAAAAATAAGTT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTITQVKIKKLENFSGSLPEYATEHSAGMDLIAANEQPITIKAAAIQLIPTGIAIALPDSFEAQIRPRSGLAVKHGITVA
NSPGTIDADYRGEIKVILINLGKEDFIIEKGMRIAQMIIAKYERILWEESSSLMETMRGSGGFGSTGV

Sequences:

>Translated_148_residues
MTITQVKIKKLENFSGSLPEYATEHSAGMDLIAANEQPITIKAAAIQLIPTGIAIALPDSFEAQIRPRSGLAVKHGITVA
NSPGTIDADYRGEIKVILINLGKEDFIIEKGMRIAQMIIAKYERILWEESSSLMETMRGSGGFGSTGV
>Mature_147_residues
TITQVKIKKLENFSGSLPEYATEHSAGMDLIAANEQPITIKAAAIQLIPTGIAIALPDSFEAQIRPRSGLAVKHGITVAN
SPGTIDADYRGEIKVILINLGKEDFIIEKGMRIAQMIIAKYERILWEESSSLMETMRGSGGFGSTGV

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=146, Percent_Identity=43.8356164383562, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI70906444, Length=146, Percent_Identity=43.8356164383562, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI70906441, Length=146, Percent_Identity=43.8356164383562, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1790071, Length=131, Percent_Identity=41.9847328244275, Blast_Score=101, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988561, Length=142, Percent_Identity=44.3661971830986, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=153, Percent_Identity=41.8300653594771, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=38.3458646616541, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19921126, Length=133, Percent_Identity=37.593984962406, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_RICAE (C3PNB9)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845172.1
- ProteinModelPortal:   C3PNB9
- SMR:   C3PNB9
- GeneID:   7815574
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0508
- OMA:   HGIALVN
- ProtClustDB:   PRK00601
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15976; Mature: 15845

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITQVKIKKLENFSGSLPEYATEHSAGMDLIAANEQPITIKAAAIQLIPTGIAIALPDS
CCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCEEEEEEEEEEECCCEEEECCCC
FEAQIRPRSGLAVKHGITVANSPGTIDADYRGEIKVILINLGKEDFIIEKGMRIAQMIIA
CCEEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHH
KYERILWEESSSLMETMRGSGGFGSTGV
HHHHHHHHCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TITQVKIKKLENFSGSLPEYATEHSAGMDLIAANEQPITIKAAAIQLIPTGIAIALPDS
CCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCCCEEEEEEEEEEECCCEEEECCCC
FEAQIRPRSGLAVKHGITVANSPGTIDADYRGEIKVILINLGKEDFIIEKGMRIAQMIIA
CCEEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHH
KYERILWEESSSLMETMRGSGGFGSTGV
HHHHHHHHCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA