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The map label for this gene is yjcC [H]

Identifier: 229586495

GI number: 229586495

Start: 332712

End: 333938

Strand: Direct

Name: yjcC [H]

Synonym: RAF_ORF0307

Alternate gene names: 229586495

Gene position: 332712-333938 (Clockwise)

Preceding gene: 229586492

Following gene: 229586496

Centisome position: 26.02

GC content: 27.71

Gene sequence:

>1227_bases
ATGATACACATGGCAATATCAGAATTAAGAACGAATTATAAAGATTTACAAGATGCGATAGATAAATTGGAAAAAGGCGT
ATGTTTTGCTTGTAGATTAGTTAATTATGATGAAATAGAATTTATAGAGCAGGGTAAGCATTTTATAATTGAAGATTTAG
ATCAAATCATTGAAGATGTAAGCAAAGAACTTAATATTTTTTCAAAGTTTAAGAAAATAAAGAAAGATAGAATATTATTT
ATCCTAAATACTACTGATTCTGACTTAATTAAAAATTTTGCTAGAAAATTATATTTATTTTTGCAGCTTTATATTAATGA
ACAGAAGCCGGCAATTTATATGAATTGTTATATTGCTAGTATTAAATTTCCAAAAAAAGTAAGTAATAATGCTAAAGAAA
TTGAAAAAACATTAAATATGTTACTTTCACAAAATAATAATTATTATTACCGTGAATATAGCAGTACAGAACATGATTTG
GAAAATATAAGAAAATCTAATCTTCAGCTTAACTTACTACGACAAGCCTTAGCAAAAAAGACTATGCGATTTGCTTATCA
GCCTATAATAGATCGTAATACTATGAAGATTCATTATTATGAATGTCTGCTTCGTATACCTGACGAGAACGGTGTTTATA
TTTCCGTAGGTCCTATAATCCCTATTGCTGAAAATAAAGGATTAATTTTTATAATTGATCAAATTGTTTTAGAAATGACT
GTTAATGAACTTGCCCGTAACCCAAACTTAATGTTAGCCTTTAATATTTCAAATATAGGAACATGCGATGAAGCTTTATG
GGAAATAGCAGAAAACTTATTAAAAGCTCATAATGTTGGGGATCGTTTAATTATTGAAATTACGGAAACTTCTTTCAATC
AGAATTACGATAAAATAACTTTATTTATTAATAAAATACATAAGTACGGATGTAAATTTGCATTAGACGATTTTGGTGCG
GGTTTTACTTCTTTTAAACAACTTCAAAGCTTACCAATTGATATTATCAAAATTGACGGTAAATATGTGCGTAGTATTAC
AAGTGATGTACAAAGTAGGTATTTCGTAGAAAGATTAATTAAAATTTCAGAAGATTTAGGTATTGAAACGGTAGCTGAAT
TTGTAGAAAACGGAGAAATAGCTAAATTTTTAATTGATCTAAAAGTCGGTGGGCTACAAGGAAACTTCTATTCCGAAGCA
AAATTTGATAGGGTGGATGAGGTTTAA

Upstream 100 bases:

>100_bases
GTTTTAACACACTTAATTTTTCTTGTATCACTTCTATGCGGTTCATATTTTTATTTCATTTACAGTAAAAATGCCCTAAT
ATTCTTAAGTATATAATTTT

Downstream 100 bases:

>100_bases
TGATTGTCATACTGAACTGGTTTTGTTGTATGGCTCTTGAAAAAGACTCCGATGTTATGCTGTGATTTGACCACGGTATC
CATAAAAATAACTTTTAATA

Product: EAL domain containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MIHMAISELRTNYKDLQDAIDKLEKGVCFACRLVNYDEIEFIEQGKHFIIEDLDQIIEDVSKELNIFSKFKKIKKDRILF
ILNTTDSDLIKNFARKLYLFLQLYINEQKPAIYMNCYIASIKFPKKVSNNAKEIEKTLNMLLSQNNNYYYREYSSTEHDL
ENIRKSNLQLNLLRQALAKKTMRFAYQPIIDRNTMKIHYYECLLRIPDENGVYISVGPIIPIAENKGLIFIIDQIVLEMT
VNELARNPNLMLAFNISNIGTCDEALWEIAENLLKAHNVGDRLIIEITETSFNQNYDKITLFINKIHKYGCKFALDDFGA
GFTSFKQLQSLPIDIIKIDGKYVRSITSDVQSRYFVERLIKISEDLGIETVAEFVENGEIAKFLIDLKVGGLQGNFYSEA
KFDRVDEV

Sequences:

>Translated_408_residues
MIHMAISELRTNYKDLQDAIDKLEKGVCFACRLVNYDEIEFIEQGKHFIIEDLDQIIEDVSKELNIFSKFKKIKKDRILF
ILNTTDSDLIKNFARKLYLFLQLYINEQKPAIYMNCYIASIKFPKKVSNNAKEIEKTLNMLLSQNNNYYYREYSSTEHDL
ENIRKSNLQLNLLRQALAKKTMRFAYQPIIDRNTMKIHYYECLLRIPDENGVYISVGPIIPIAENKGLIFIIDQIVLEMT
VNELARNPNLMLAFNISNIGTCDEALWEIAENLLKAHNVGDRLIIEITETSFNQNYDKITLFINKIHKYGCKFALDDFGA
GFTSFKQLQSLPIDIIKIDGKYVRSITSDVQSRYFVERLIKISEDLGIETVAEFVENGEIAKFLIDLKVGGLQGNFYSEA
KFDRVDEV
>Mature_408_residues
MIHMAISELRTNYKDLQDAIDKLEKGVCFACRLVNYDEIEFIEQGKHFIIEDLDQIIEDVSKELNIFSKFKKIKKDRILF
ILNTTDSDLIKNFARKLYLFLQLYINEQKPAIYMNCYIASIKFPKKVSNNAKEIEKTLNMLLSQNNNYYYREYSSTEHDL
ENIRKSNLQLNLLRQALAKKTMRFAYQPIIDRNTMKIHYYECLLRIPDENGVYISVGPIIPIAENKGLIFIIDQIVLEMT
VNELARNPNLMLAFNISNIGTCDEALWEIAENLLKAHNVGDRLIIEITETSFNQNYDKITLFINKIHKYGCKFALDDFGA
GFTSFKQLQSLPIDIIKIDGKYVRSITSDVQSRYFVERLIKISEDLGIETVAEFVENGEIAKFLIDLKVGGLQGNFYSEA
KFDRVDEV

Specific function: Overexpression reduces biofilm formation [H]

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1790496, Length=239, Percent_Identity=32.2175732217573, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788849, Length=231, Percent_Identity=31.6017316017316, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786507, Length=233, Percent_Identity=28.755364806867, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081743, Length=246, Percent_Identity=26.8292682926829, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081921, Length=232, Percent_Identity=28.448275862069, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787541, Length=358, Percent_Identity=24.8603351955307, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081980, Length=257, Percent_Identity=29.1828793774319, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082096, Length=276, Percent_Identity=27.8985507246377, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081845, Length=235, Percent_Identity=29.3617021276596, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI226510982, Length=220, Percent_Identity=28.1818181818182, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788502, Length=233, Percent_Identity=25.3218884120172, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787055, Length=240, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787410, Length=233, Percent_Identity=23.6051502145923, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 47399; Mature: 47399

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHMAISELRTNYKDLQDAIDKLEKGVCFACRLVNYDEIEFIEQGKHFIIEDLDQIIEDV
CCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHH
SKELNIFSKFKKIKKDRILFILNTTDSDLIKNFARKLYLFLQLYINEQKPAIYMNCYIAS
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
IKFPKKVSNNAKEIEKTLNMLLSQNNNYYYREYSSTEHDLENIRKSNLQLNLLRQALAKK
EECCHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHH
TMRFAYQPIIDRNTMKIHYYECLLRIPDENGVYISVGPIIPIAENKGLIFIIDQIVLEMT
HHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEEEECCEEEEECCCCEEEEHHHHHHHHH
VNELARNPNLMLAFNISNIGTCDEALWEIAENLLKAHNVGDRLIIEITETSFNQNYDKIT
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
LFINKIHKYGCKFALDDFGAGFTSFKQLQSLPIDIIKIDGKYVRSITSDVQSRYFVERLI
HHHHHHHHHCCCEEEHHCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
KISEDLGIETVAEFVENGEIAKFLIDLKVGGLQGNFYSEAKFDRVDEV
HHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIHMAISELRTNYKDLQDAIDKLEKGVCFACRLVNYDEIEFIEQGKHFIIEDLDQIIEDV
CCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHH
SKELNIFSKFKKIKKDRILFILNTTDSDLIKNFARKLYLFLQLYINEQKPAIYMNCYIAS
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEE
IKFPKKVSNNAKEIEKTLNMLLSQNNNYYYREYSSTEHDLENIRKSNLQLNLLRQALAKK
EECCHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHH
TMRFAYQPIIDRNTMKIHYYECLLRIPDENGVYISVGPIIPIAENKGLIFIIDQIVLEMT
HHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEEEECCEEEEECCCCEEEEHHHHHHHHH
VNELARNPNLMLAFNISNIGTCDEALWEIAENLLKAHNVGDRLIIEITETSFNQNYDKIT
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
LFINKIHKYGCKFALDDFGAGFTSFKQLQSLPIDIIKIDGKYVRSITSDVQSRYFVERLI
HHHHHHHHHCCCEEEHHCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
KISEDLGIETVAEFVENGEIAKFLIDLKVGGLQGNFYSEAKFDRVDEV
HHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]