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The map label for this gene is emrA [H]

Identifier: 229586492

GI number: 229586492

Start: 330345

End: 331397

Strand: Direct

Name: emrA [H]

Synonym: RAF_ORF0304

Alternate gene names: 229586492

Gene position: 330345-331397 (Clockwise)

Preceding gene: 229586491

Following gene: 229586495

Centisome position: 25.84

GC content: 33.05

Gene sequence:

>1053_bases
ATGCCGAGTGTATTACAACAAGCTATCGACAAATATAAACATCATCCATTTACTAAATATGTAATTATTATAGCCTTAAT
CATATTTATTTATTTGGGATGTAGAATTTATGTTTGGGCAAATACACAATCTACGGATAATGCTTACATAGATGCAGATA
TTTCAAATGTTAGTGCTGAAGTTAGCGGTGTTTTAACAAAGTTATTTGTTACCGATAATACTAAAGTTAATAAAGGCGAT
CTGATAGGTGAAATTGACGATAGAGATTATAAGGCAAAGCTTGCAGCACTTGAAGCATCTATTGAAGCTTGCATAAAAAA
TATAGAAATTATAGAGCAAAAAATCTCAATAGGGCAGATTAGCCTAGAGCAAGCAGCTGAAAAATTAAAGCTTAATAAAA
TAAGCTTTGATATTGTTGCAACAGATTTTCTGAGAGTGCAGGAGTTAAATAAGGCAAAATTTGCTAGCTCTAAAACGTTA
GATGATTCTAAAAACGCATATCAAAAAGCCAAAACGGATTATAAACAAGCACAGTTAGATTTAGATATATCTAAGCAACA
TTTACAGCTTCTTGAGCTTGAAAAAGCAGCAGAACAGGAAAAACTTAAAGAGCTAACAGAAAATAAAAAAGTAACCTTAA
GGAGCTTACAAAATACTAAACTTATTGCTATGGTACCCGGTATATTCGGTAATAGTAGTTTAGAAGTCGGAAATTACATA
GTACCGGGTAGGGTGTTGTTTTCTATAGTTCAAGATAATACTATGTATATTCAGGCTAATTTTAAAGAAACACAAATTCA
GAAATTTAAGTCCGGTATGAAAGTCAAAATTGAATTTGATGCTTTGCCTAAAAAGGTAATTTACGGGAAGATTCGTAATA
TTGCTCCTGCGACCGGCTCTAAATTTAGCTTAATCCCACCAGATCATGCTACCGGTAATTTTACTAAAAGCGTACAACGT
GTACCTGTTTTAATAGATTTTGAATCCCCAAATGCTAATCTAGTCCCCGGCATGTCAGCAATCGTATCCATTAGAACGGA
TCAAAGTACGTAA

Upstream 100 bases:

>100_bases
TATATCCATGGTATTCACTGCCGTTAATTGCAGTTATATATATTCTTTCTATACCAATTTGTTATTTTTTTAAACATAGA
GGGTATTGGTAAAGTTAATT

Downstream 100 bases:

>100_bases
TTTTTGTTAAAAAATAGTAATAATTATTCTATTAAAAGTACATATTCTAGTATTATTTACTGATTACTTAAAAAGCTATA
TAATATGAGTAATACCAAAA

Product: Multidrug resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MPSVLQQAIDKYKHHPFTKYVIIIALIIFIYLGCRIYVWANTQSTDNAYIDADISNVSAEVSGVLTKLFVTDNTKVNKGD
LIGEIDDRDYKAKLAALEASIEACIKNIEIIEQKISIGQISLEQAAEKLKLNKISFDIVATDFLRVQELNKAKFASSKTL
DDSKNAYQKAKTDYKQAQLDLDISKQHLQLLELEKAAEQEKLKELTENKKVTLRSLQNTKLIAMVPGIFGNSSLEVGNYI
VPGRVLFSIVQDNTMYIQANFKETQIQKFKSGMKVKIEFDALPKKVIYGKIRNIAPATGSKFSLIPPDHATGNFTKSVQR
VPVLIDFESPNANLVPGMSAIVSIRTDQST

Sequences:

>Translated_350_residues
MPSVLQQAIDKYKHHPFTKYVIIIALIIFIYLGCRIYVWANTQSTDNAYIDADISNVSAEVSGVLTKLFVTDNTKVNKGD
LIGEIDDRDYKAKLAALEASIEACIKNIEIIEQKISIGQISLEQAAEKLKLNKISFDIVATDFLRVQELNKAKFASSKTL
DDSKNAYQKAKTDYKQAQLDLDISKQHLQLLELEKAAEQEKLKELTENKKVTLRSLQNTKLIAMVPGIFGNSSLEVGNYI
VPGRVLFSIVQDNTMYIQANFKETQIQKFKSGMKVKIEFDALPKKVIYGKIRNIAPATGSKFSLIPPDHATGNFTKSVQR
VPVLIDFESPNANLVPGMSAIVSIRTDQST
>Mature_349_residues
PSVLQQAIDKYKHHPFTKYVIIIALIIFIYLGCRIYVWANTQSTDNAYIDADISNVSAEVSGVLTKLFVTDNTKVNKGDL
IGEIDDRDYKAKLAALEASIEACIKNIEIIEQKISIGQISLEQAAEKLKLNKISFDIVATDFLRVQELNKAKFASSKTLD
DSKNAYQKAKTDYKQAQLDLDISKQHLQLLELEKAAEQEKLKELTENKKVTLRSLQNTKLIAMVPGIFGNSSLEVGNYIV
PGRVLFSIVQDNTMYIQANFKETQIQKFKSGMKVKIEFDALPKKVIYGKIRNIAPATGSKFSLIPPDHATGNFTKSVQRV
PVLIDFESPNANLVPGMSAIVSIRTDQST

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=331, Percent_Identity=27.4924471299094, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788711, Length=329, Percent_Identity=27.6595744680851, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1790519, Length=323, Percent_Identity=28.1733746130031, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790024, Length=254, Percent_Identity=22.4409448818898, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39065; Mature: 38933

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVLQQAIDKYKHHPFTKYVIIIALIIFIYLGCRIYVWANTQSTDNAYIDADISNVSAE
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECHHHHHHH
VSGVLTKLFVTDNTKVNKGDLIGEIDDRDYKAKLAALEASIEACIKNIEIIEQKISIGQI
HHHHEEEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SLEQAAEKLKLNKISFDIVATDFLRVQELNKAKFASSKTLDDSKNAYQKAKTDYKQAQLD
EHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
LDISKQHLQLLELEKAAEQEKLKELTENKKVTLRSLQNTKLIAMVPGIFGNSSLEVGNYI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCEECCCEE
VPGRVLFSIVQDNTMYIQANFKETQIQKFKSGMKVKIEFDALPKKVIYGKIRNIAPATGS
CCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCC
KFSLIPPDHATGNFTKSVQRVPVLIDFESPNANLVPGMSAIVSIRTDQST
EEEECCCCCCCCCHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
PSVLQQAIDKYKHHPFTKYVIIIALIIFIYLGCRIYVWANTQSTDNAYIDADISNVSAE
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECHHHHHHH
VSGVLTKLFVTDNTKVNKGDLIGEIDDRDYKAKLAALEASIEACIKNIEIIEQKISIGQI
HHHHEEEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SLEQAAEKLKLNKISFDIVATDFLRVQELNKAKFASSKTLDDSKNAYQKAKTDYKQAQLD
EHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
LDISKQHLQLLELEKAAEQEKLKELTENKKVTLRSLQNTKLIAMVPGIFGNSSLEVGNYI
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCCEECCCEE
VPGRVLFSIVQDNTMYIQANFKETQIQKFKSGMKVKIEFDALPKKVIYGKIRNIAPATGS
CCHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCC
KFSLIPPDHATGNFTKSVQRVPVLIDFESPNANLVPGMSAIVSIRTDQST
EEEECCCCCCCCCHHHHHHHCEEEEEECCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]