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The map label for this gene is holB [C]

Identifier: 229586414

GI number: 229586414

Start: 224219

End: 225013

Strand: Direct

Name: holB [C]

Synonym: RAF_ORF0205

Alternate gene names: 229586414

Gene position: 224219-225013 (Clockwise)

Preceding gene: 229586413

Following gene: 229586415

Centisome position: 17.54

GC content: 26.79

Gene sequence:

>795_bases
TTGATAATTGAACGCCTAGAGTTTAACTTAAAACATAATAAGCTATACAATAGTTGGCTTATCGAAGCCGAAAATATAGA
GCAAGCTTTAAAAGATTTAGAAGAGTTTATATATATTAAGCTTTTTAAGAATAGCATTCCTCTTGACAACAATCCTGATT
ATCATTTCATCGCTAGGGAAACTTCAGCTACATCTAATGCTAAAAATATTTCTATTGAACAAATAAGAAAATTACAAGAC
TTCTTAAGTAAAACTTCTGCAATTTCAGGTTATAAAGTTGCAATAATTTATTCGGCAGAGCTTATGAATTTGAATGCAGC
GAATTCATGCTTAAAAATTCTTGAAGATGCACCAAAGAATAGTTACATTTTTTTAATTACTTCAAGAGCTGCAAGTATTA
TTTCTACAATTAGATCAAGATGTTTTAAAATTAATGTTAGATCATCTATCCATCATACTAAAAACGAATTATATTCCGAG
TTTATTCAGTCGATAGCAGATAACAAAACGTTAGATTTTATTCATCGCTTTACTACTAAAGACCGAGAATTATGGCTAGA
TTTTATTGATAATTTATTATTATTAATGAATAGAATCCTTAAAAAATCTGCCAACGTTAATATTGAACTTCTAGATTTGG
AAAATAAGATTTTTAATAAACTATCAAATAGACACCCTTCATATTTACTGCAAAAATTTACCGACATAAAAAAGTTAATA
TATAATACCATTGATTATGATTTGGACTTAAAAACAAGTTATATATTAGTGGTGAATGAGTTTTCTAGTAATTAA

Upstream 100 bases:

>100_bases
GGGGTGACACTAGAAAAACCAGCTCATGCAATAATGCTCTGCAAAAGCCGGAATGATATAGAATACTTCTTAAGTACAAT
TCATAATGATATTACAACCA

Downstream 100 bases:

>100_bases
CACGATATCACTACGTTCCTTTCTATGTTATCCATAAAACAACAAAATATATTGAGATACTCTTTTTTTTTATAGTTAAT
AGATATTCAAGATAGAAAGC

Product: DNA polymerase III subunit delta'

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIIERLEFNLKHNKLYNSWLIEAENIEQALKDLEEFIYIKLFKNSIPLDNNPDYHFIARETSATSNAKNISIEQIRKLQD
FLSKTSAISGYKVAIIYSAELMNLNAANSCLKILEDAPKNSYIFLITSRAASIISTIRSRCFKINVRSSIHHTKNELYSE
FIQSIADNKTLDFIHRFTTKDRELWLDFIDNLLLLMNRILKKSANVNIELLDLENKIFNKLSNRHPSYLLQKFTDIKKLI
YNTIDYDLDLKTSYILVVNEFSSN

Sequences:

>Translated_264_residues
MIIERLEFNLKHNKLYNSWLIEAENIEQALKDLEEFIYIKLFKNSIPLDNNPDYHFIARETSATSNAKNISIEQIRKLQD
FLSKTSAISGYKVAIIYSAELMNLNAANSCLKILEDAPKNSYIFLITSRAASIISTIRSRCFKINVRSSIHHTKNELYSE
FIQSIADNKTLDFIHRFTTKDRELWLDFIDNLLLLMNRILKKSANVNIELLDLENKIFNKLSNRHPSYLLQKFTDIKKLI
YNTIDYDLDLKTSYILVVNEFSSN
>Mature_264_residues
MIIERLEFNLKHNKLYNSWLIEAENIEQALKDLEEFIYIKLFKNSIPLDNNPDYHFIARETSATSNAKNISIEQIRKLQD
FLSKTSAISGYKVAIIYSAELMNLNAANSCLKILEDAPKNSYIFLITSRAASIISTIRSRCFKINVRSSIHHTKNELYSE
FIQSIADNKTLDFIHRFTTKDRELWLDFIDNLLLLMNRILKKSANVNIELLDLENKIFNKLSNRHPSYLLQKFTDIKKLI
YNTIDYDLDLKTSYILVVNEFSSN

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. [C]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 30843; Mature: 30843

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIERLEFNLKHNKLYNSWLIEAENIEQALKDLEEFIYIKLFKNSIPLDNNPDYHFIARE
CCCCCEECCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
TSATSNAKNISIEQIRKLQDFLSKTSAISGYKVAIIYSAELMNLNAANSCLKILEDAPKN
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHCCCHHHHHHHHHHHCCCC
SYIFLITSRAASIISTIRSRCFKINVRSSIHHTKNELYSEFIQSIADNKTLDFIHRFTTK
CEEEEEECHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DRELWLDFIDNLLLLMNRILKKSANVNIELLDLENKIFNKLSNRHPSYLLQKFTDIKKLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHH
YNTIDYDLDLKTSYILVVNEFSSN
HHHCCCCEECCCEEEEEEECCCCC
>Mature Secondary Structure
MIIERLEFNLKHNKLYNSWLIEAENIEQALKDLEEFIYIKLFKNSIPLDNNPDYHFIARE
CCCCCEECCCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
TSATSNAKNISIEQIRKLQDFLSKTSAISGYKVAIIYSAELMNLNAANSCLKILEDAPKN
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHCCCHHHHHHHHHHHCCCC
SYIFLITSRAASIISTIRSRCFKINVRSSIHHTKNELYSEFIQSIADNKTLDFIHRFTTK
CEEEEEECHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
DRELWLDFIDNLLLLMNRILKKSANVNIELLDLENKIFNKLSNRHPSYLLQKFTDIKKLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHH
YNTIDYDLDLKTSYILVVNEFSSN
HHHCCCCEECCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA