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The map label for this gene is rlmL [H]

Identifier: 228873813

GI number: 228873813

Start: 1436208

End: 1438325

Strand: Direct

Name: rlmL [H]

Synonym: VF_1287

Alternate gene names: 228873813

Gene position: 1436208-1438325 (Clockwise)

Preceding gene: 59711893

Following gene: 59711895

Centisome position: 49.57

GC content: 41.03

Gene sequence:

>2118_bases
ATGAAAAAATACTTAGCCATTACTTCTAAAGGCCTTGAAAACTTACTTGCAGATGAACTGATTGCACTTGGTGTGACTGA
CCCTAAAGTGGTTCACGCTGGTGTTAAATTCGAAGCACCAATCGAGGTCGTTTATCGTTGTTGTTTGTGGAGCCGTATTG
CTTCACGTTTCATTCAAATTCTTTCTGAGTTCGATGTCCGTGATGATATGGACTTATACCTAGGCGCATCTTCAATTAAC
TGGCCTAGTTATTTCTCTGCTGATAAAACGCTAGTGGTGGACTTTAACGGTACTAACCGTGAGATTCGAAATAGCCAATA
CGGTGCATTAAAAGTAAAAGATGCAATTGTTGACCGTTTTACAAAAGCGGACTTAGCGCGTCCAAATATCAGTAAAGTAG
AACCAGACTTACGTGTTCATATGCGTTTATCAGGCGAGAAAGGGATTCTTGGCTTTGATTTAGTGGGTTCGGGTTTGCAC
CAACGAGGCTACCGTACTGAAGCCGGTCGTGCGCCACTTCGTGAAACTCTAGCAGCAGCACTTGTTATGCGTAGTACATG
GGATGAGTCAAAACCTCTACTAGATCCAATGTGTGGTTCTGGTACCTTACTGATTGAAGCGGCACTAATGGCATGTGAAA
TGGCTCCTGGTGTTAAGCGTGAGAAGTGGTGTTTTGAAGCACTAAATGATTTTGACGCTGAGCTTTGGGCTGAGATTCGT
TCTGAGGCTCGTGTTAAGAGTCGCCGTGGCGTTAAGAAAGTAGATGCTCGTTTCTACGGCTTTGATCGTGATTACCGTGT
TATTCAAACCGCTCGTGAAAATGCGCGTCGTGCTGGTGTTGAAGATTTAATTACTTTTGATGTGGGCGATGCTACGAAAG
TTGAGTGCCCAGAAGGGTTTGAGAATGGTGTTATTCTTTGTAACCCTCCTTATGGTGAGCGTCTAAGTACAGAGCCTGCA
TTGATTGCACTGTACAGCGAATTTGGTCGCCAATTAAAAGAAGTATTTGGCGGTTGTACGGCATCTATCTATTCAAGCAA
TGATGATCTACTTGCTTGTTTACGTATGCGTGCAGATAAGCAATTTAAATTGAACAATGGTGCATTACCTTGTGTTCAGA
AGAACTACTCAATTACTGAATCTGCTGAACGTAAAGAAGCGGTAAGCATTGAAGTTGCTCCTGAGTTCATGAACCGTCTT
AAAAAGAACATTGGCAAGATTGGTAAATGGGCACGTAAAGAGAAGTTAGATTGTTACCGTATCTACGATGCTGACTTACC
AGATTACAATGCAGCAATTGATGTTTATAAAGATTACATCATCATTCAAGAATACGCGGCACCAAAAACGATTTCAGAAG
ATAAAGCACGTCGTCGTTTAACGGATATGATCCGTGCAACGGTTCTAGTAACGGGTGTTGAAACTAATAACGTTATTTTA
AAAGTTCGTCAAAAACAATCTGGTAAAAATCAGTATCAAAAACTGGCAGAGAAATCTCGTTACTTTGATGTTGAAGAGTA
CGGTGTCAAACTGATTGTTAACCTACAAGATTACCTAGATACCGGTTTATTCTTAGACCATAAGTTGACTCGTAAAATGT
TAGGTGAAATAGCAGCAGGAAAAGACTTCCTAAACCTATTTGCTTACACAGGTTCTGCAACTGTTCACGCTGCTTGTGGT
GGTGCTAAATCTACCATGACGATTGATATGTCTCGTACCTACTTAGAGTGGGCGCAGAAGAACATGAATACCAATGGTCA
AACGGGTACACAACACCAGTTCTTGCAAGCTGATTGTTTACAATGGTTACAACAAGCGGACGGTGAATTCGATTTAATCT
TTATCGATCCACCAACGTTCTCTAACTCTAAGCGTATGGAACAAACGTTTGATGTACAGCGTGATCACATTATGCTGCTT
GAAAACTTAAAACGTATGCTACGTGAGAACGGTACTATTGTATTCTCTAACAACAAACGTAACTTCAAAATGGATGAAGC
AGGATTAGAGAAAGCAGGGCTTAAAGCGAAGAATATTTCTAAGCAAACTTTGCCTCTTGATTTTGCTCGTAACAAGCATA
TCCATAACTGTTGGATTATTACCCACAAGGAAGACTAA

Upstream 100 bases:

>100_bases
TTAAGTGGATAAAAGAGTTGTCATTTATCTGCTATCTAAATGTCCTATTTCATATAGATTCATGTATAATGCGAGGCAAT
TTAATAACGAAAAGAATCCC

Downstream 100 bases:

>100_bases
GAGATAATGATCACTCTCTATAGCACGGAAGGCTGCCACCTTTGCGAGCAAGCGTTTGACTTGCTTGTCGAGGTGGGTGT
AGATATGGAACAAGTAGATA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKKYLAITSKGLENLLADELIALGVTDPKVVHAGVKFEAPIEVVYRCCLWSRIASRFIQILSEFDVRDDMDLYLGASSIN
WPSYFSADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRFTKADLARPNISKVEPDLRVHMRLSGEKGILGFDLVGSGLH
QRGYRTEAGRAPLRETLAAALVMRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEALNDFDAELWAEIR
SEARVKSRRGVKKVDARFYGFDRDYRVIQTARENARRAGVEDLITFDVGDATKVECPEGFENGVILCNPPYGERLSTEPA
LIALYSEFGRQLKEVFGGCTASIYSSNDDLLACLRMRADKQFKLNNGALPCVQKNYSITESAERKEAVSIEVAPEFMNRL
KKNIGKIGKWARKEKLDCYRIYDADLPDYNAAIDVYKDYIIIQEYAAPKTISEDKARRRLTDMIRATVLVTGVETNNVIL
KVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMLGEIAAGKDFLNLFAYTGSATVHAACG
GAKSTMTIDMSRTYLEWAQKNMNTNGQTGTQHQFLQADCLQWLQQADGEFDLIFIDPPTFSNSKRMEQTFDVQRDHIMLL
ENLKRMLRENGTIVFSNNKRNFKMDEAGLEKAGLKAKNISKQTLPLDFARNKHIHNCWIITHKED

Sequences:

>Translated_705_residues
MKKYLAITSKGLENLLADELIALGVTDPKVVHAGVKFEAPIEVVYRCCLWSRIASRFIQILSEFDVRDDMDLYLGASSIN
WPSYFSADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRFTKADLARPNISKVEPDLRVHMRLSGEKGILGFDLVGSGLH
QRGYRTEAGRAPLRETLAAALVMRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEALNDFDAELWAEIR
SEARVKSRRGVKKVDARFYGFDRDYRVIQTARENARRAGVEDLITFDVGDATKVECPEGFENGVILCNPPYGERLSTEPA
LIALYSEFGRQLKEVFGGCTASIYSSNDDLLACLRMRADKQFKLNNGALPCVQKNYSITESAERKEAVSIEVAPEFMNRL
KKNIGKIGKWARKEKLDCYRIYDADLPDYNAAIDVYKDYIIIQEYAAPKTISEDKARRRLTDMIRATVLVTGVETNNVIL
KVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMLGEIAAGKDFLNLFAYTGSATVHAACG
GAKSTMTIDMSRTYLEWAQKNMNTNGQTGTQHQFLQADCLQWLQQADGEFDLIFIDPPTFSNSKRMEQTFDVQRDHIMLL
ENLKRMLRENGTIVFSNNKRNFKMDEAGLEKAGLKAKNISKQTLPLDFARNKHIHNCWIITHKED
>Mature_705_residues
MKKYLAITSKGLENLLADELIALGVTDPKVVHAGVKFEAPIEVVYRCCLWSRIASRFIQILSEFDVRDDMDLYLGASSIN
WPSYFSADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRFTKADLARPNISKVEPDLRVHMRLSGEKGILGFDLVGSGLH
QRGYRTEAGRAPLRETLAAALVMRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEALNDFDAELWAEIR
SEARVKSRRGVKKVDARFYGFDRDYRVIQTARENARRAGVEDLITFDVGDATKVECPEGFENGVILCNPPYGERLSTEPA
LIALYSEFGRQLKEVFGGCTASIYSSNDDLLACLRMRADKQFKLNNGALPCVQKNYSITESAERKEAVSIEVAPEFMNRL
KKNIGKIGKWARKEKLDCYRIYDADLPDYNAAIDVYKDYIIIQEYAAPKTISEDKARRRLTDMIRATVLVTGVETNNVIL
KVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMLGEIAAGKDFLNLFAYTGSATVHAACG
GAKSTMTIDMSRTYLEWAQKNMNTNGQTGTQHQFLQADCLQWLQQADGEFDLIFIDPPTFSNSKRMEQTFDVQRDHIMLL
ENLKRMLRENGTIVFSNNKRNFKMDEAGLEKAGLKAKNISKQTLPLDFARNKHIHNCWIITHKED

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=704, Percent_Identity=56.3920454545455, Blast_Score=827, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=245, Percent_Identity=27.7551020408163, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 79883; Mature: 79883

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYLAITSKGLENLLADELIALGVTDPKVVHAGVKFEAPIEVVYRCCLWSRIASRFIQI
CCCEEEEHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LSEFDVRDDMDLYLGASSINWPSYFSADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCEEEHHHHHHHHH
TKADLARPNISKVEPDLRVHMRLSGEKGILGFDLVGSGLHQRGYRTEAGRAPLRETLAAA
HHHHCCCCCHHHCCCCEEEEEEECCCCCEEEHHHHCCCHHHCCCCCCCCCCHHHHHHHHH
LVMRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEALNDFDAELWAEIR
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
SEARVKSRRGVKKVDARFYGFDRDYRVIQTARENARRAGVEDLITFDVGDATKVECPEGF
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCC
ENGVILCNPPYGERLSTEPALIALYSEFGRQLKEVFGGCTASIYSSNDDLLACLRMRADK
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCC
QFKLNNGALPCVQKNYSITESAERKEAVSIEVAPEFMNRLKKNIGKIGKWARKEKLDCYR
CEEECCCCCCCCCCCCCCCCCHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEE
IYDADLPDYNAAIDVYKDYIIIQEYAAPKTISEDKARRRLTDMIRATVLVTGVETNNVIL
EECCCCCCCCHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCEEE
KVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMLGEIAAG
EEECCCCCHHHHHHHHHHHCCCCHHHCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHCC
KDFLNLFAYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNGQTGTQHQFLQADCL
HHHHHHHHCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QWLQQADGEFDLIFIDPPTFSNSKRMEQTFDVQRDHIMLLENLKRMLRENGTIVFSNNKR
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCC
NFKMDEAGLEKAGLKAKNISKQTLPLDFARNKHIHNCWIITHKED
CCCCCHHCHHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEEEECCC
>Mature Secondary Structure
MKKYLAITSKGLENLLADELIALGVTDPKVVHAGVKFEAPIEVVYRCCLWSRIASRFIQI
CCCEEEEHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LSEFDVRDDMDLYLGASSINWPSYFSADKTLVVDFNGTNREIRNSQYGALKVKDAIVDRF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCEEEHHHHHHHHH
TKADLARPNISKVEPDLRVHMRLSGEKGILGFDLVGSGLHQRGYRTEAGRAPLRETLAAA
HHHHCCCCCHHHCCCCEEEEEEECCCCCEEEHHHHCCCHHHCCCCCCCCCCHHHHHHHHH
LVMRSTWDESKPLLDPMCGSGTLLIEAALMACEMAPGVKREKWCFEALNDFDAELWAEIR
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
SEARVKSRRGVKKVDARFYGFDRDYRVIQTARENARRAGVEDLITFDVGDATKVECPEGF
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCC
ENGVILCNPPYGERLSTEPALIALYSEFGRQLKEVFGGCTASIYSSNDDLLACLRMRADK
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCC
QFKLNNGALPCVQKNYSITESAERKEAVSIEVAPEFMNRLKKNIGKIGKWARKEKLDCYR
CEEECCCCCCCCCCCCCCCCCHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEE
IYDADLPDYNAAIDVYKDYIIIQEYAAPKTISEDKARRRLTDMIRATVLVTGVETNNVIL
EECCCCCCCCHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCEEE
KVRQKQSGKNQYQKLAEKSRYFDVEEYGVKLIVNLQDYLDTGLFLDHKLTRKMLGEIAAG
EEECCCCCHHHHHHHHHHHCCCCHHHCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHCC
KDFLNLFAYTGSATVHAACGGAKSTMTIDMSRTYLEWAQKNMNTNGQTGTQHQFLQADCL
HHHHHHHHCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QWLQQADGEFDLIFIDPPTFSNSKRMEQTFDVQRDHIMLLENLKRMLRENGTIVFSNNKR
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCC
NFKMDEAGLEKAGLKAKNISKQTLPLDFARNKHIHNCWIITHKED
CCCCCHHCHHHHCCCCCCCCCCCCCCHHHCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA