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The map label for this gene is ygdE [H]

Identifier: 228873812

GI number: 228873812

Start: 650571

End: 651662

Strand: Direct

Name: ygdE [H]

Synonym: VF_0593

Alternate gene names: 228873812

Gene position: 650571-651662 (Clockwise)

Preceding gene: 59711199

Following gene: 59711206

Centisome position: 22.45

GC content: 39.1

Gene sequence:

>1092_bases
GTGAAACAAGTAATGTTCTATTGTCGCTCTGGTTTTGAAAAAGAGTGTGCAGGTGAGATCCAAGATAAAGCCACTCAACT
AGAAGTGTTTGGTTTTCCTCGCCTAAAAAATAATACAGGCTATGTGCTATTTGAATGCTATCAGGAAGGCGATGCAGACC
GTTTAATCCGTGAGATTAAATTTAATGAGCTAATTTTTGCTCGTCAAATGTTTGCGGTTGCAGCTGAAGTTAGTGATCTA
CCTAAAGATGACCGTATTTCGCCGATTCTAGATGCATTATCTGATGTTGATGGTGTGCCTTGTTGTGGTGATATTCGTAT
TGAAACGCCAGATACGAATGAAGCAAAAGAACTATTAAAATTCTGCCGTAAGTTTACAGTTCCAATGCGTCAGGCTTTAC
GTGGTAAAAAAATGCTGACAGAAAGAGATTCAAACCGAATCCCTGTTTTGCATATTTGTTTCATTGCTCCGGGTCATTGT
TATGTTGGGTACTCTTACACAAACAATAACTCTCAATTCTTCATGGGTATTCCTCGTTTGAAATTCCCAGCAGATGCACC
AAGTCGTTCTACATTGAAATTGGAAGAAGCGTTTCACGTATTTATTCCAAAAGAAGAGTGGGATGATCGTTTAGCATCAG
GCATGTGGGGGGTTGATTTAGGTGCGTGTCCTGGTGGCTGGACTTATCAATTGGTTAAGCGTTCTATGTTTGTTCATGCG
ATTGATAATGGCATGATGGCTCAAAGCTTAATGGATACAGGCCAAGTGAAACACCACATGGTTGATGGTTTTAAATTTGA
GCCACCAAGAAAGAACGTAACATGGTTAATTTGTGACATGGTTGAGAAACCAGCTCGTGTTGCACACCTTATGGGTGAAT
GGCTAATTAAAGGTTGGGCAAAAGAAGCGTTATTTAACCTTAAATTACCAATGAAAGGTCGTTACGATGAGGTTCTTCAA
GATATTGAAAACCTAAAAGATTTCTTGAAGAAAAACGGTTTCCAATACAAGCTTCAAGCTAAGCATTTGTATCATGACCG
TGAAGAAATCACTGTTCATATCCAAGCGATTTCAAACATTTCTCCACATTAA

Upstream 100 bases:

>100_bases
TAAAATGGTTTGGTCCAATTACTCCATTAGGTGGATTCTGTTTTATTATCGGTTGGTTAACGCTGGCTGTTTCAACTTAT
CGTTTGACTGAGGTTTCAAA

Downstream 100 bases:

>100_bases
TATTTCGTTATATAAATATTGATGTAATCCAAATTATTGAGAAATAAAAAACGCCCGCTGATGTTGATAATCAGCGGGCG
TTTTTATTTATCTTCAACAA

Product: putative RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKNNTGYVLFECYQEGDADRLIREIKFNELIFARQMFAVAAEVSDL
PKDDRISPILDALSDVDGVPCCGDIRIETPDTNEAKELLKFCRKFTVPMRQALRGKKMLTERDSNRIPVLHICFIAPGHC
YVGYSYTNNNSQFFMGIPRLKFPADAPSRSTLKLEEAFHVFIPKEEWDDRLASGMWGVDLGACPGGWTYQLVKRSMFVHA
IDNGMMAQSLMDTGQVKHHMVDGFKFEPPRKNVTWLICDMVEKPARVAHLMGEWLIKGWAKEALFNLKLPMKGRYDEVLQ
DIENLKDFLKKNGFQYKLQAKHLYHDREEITVHIQAISNISPH

Sequences:

>Translated_363_residues
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKNNTGYVLFECYQEGDADRLIREIKFNELIFARQMFAVAAEVSDL
PKDDRISPILDALSDVDGVPCCGDIRIETPDTNEAKELLKFCRKFTVPMRQALRGKKMLTERDSNRIPVLHICFIAPGHC
YVGYSYTNNNSQFFMGIPRLKFPADAPSRSTLKLEEAFHVFIPKEEWDDRLASGMWGVDLGACPGGWTYQLVKRSMFVHA
IDNGMMAQSLMDTGQVKHHMVDGFKFEPPRKNVTWLICDMVEKPARVAHLMGEWLIKGWAKEALFNLKLPMKGRYDEVLQ
DIENLKDFLKKNGFQYKLQAKHLYHDREEITVHIQAISNISPH
>Mature_363_residues
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKNNTGYVLFECYQEGDADRLIREIKFNELIFARQMFAVAAEVSDL
PKDDRISPILDALSDVDGVPCCGDIRIETPDTNEAKELLKFCRKFTVPMRQALRGKKMLTERDSNRIPVLHICFIAPGHC
YVGYSYTNNNSQFFMGIPRLKFPADAPSRSTLKLEEAFHVFIPKEEWDDRLASGMWGVDLGACPGGWTYQLVKRSMFVHA
IDNGMMAQSLMDTGQVKHHMVDGFKFEPPRKNVTWLICDMVEKPARVAHLMGEWLIKGWAKEALFNLKLPMKGRYDEVLQ
DIENLKDFLKKNGFQYKLQAKHLYHDREEITVHIQAISNISPH

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA [H]

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789171, Length=357, Percent_Identity=62.7450980392157, Blast_Score=488, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011224
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: NA

Molecular weight: Translated: 41772; Mature: 41772

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKNNTGYVLFECYQEGDADRLIREIK
CCHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCEEECCCCEEEEEEECCCCHHHHHHHHH
FNELIFARQMFAVAAEVSDLPKDDRISPILDALSDVDGVPCCGDIRIETPDTNEAKELLK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHH
FCRKFTVPMRQALRGKKMLTERDSNRIPVLHICFIAPGHCYVGYSYTNNNSQFFMGIPRL
HHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCEEEEECCCE
KFPADAPSRSTLKLEEAFHVFIPKEEWDDRLASGMWGVDLGACPGGWTYQLVKRSMFVHA
ECCCCCCCCCEEEEECCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IDNGMMAQSLMDTGQVKHHMVDGFKFEPPRKNVTWLICDMVEKPARVAHLMGEWLIKGWA
HCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHHH
KEALFNLKLPMKGRYDEVLQDIENLKDFLKKNGFQYKLQAKHLYHDREEITVHIQAISNI
HHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCEEEEEEEECCC
SPH
CCC
>Mature Secondary Structure
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKNNTGYVLFECYQEGDADRLIREIK
CCHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCEEECCCCEEEEEEECCCCHHHHHHHHH
FNELIFARQMFAVAAEVSDLPKDDRISPILDALSDVDGVPCCGDIRIETPDTNEAKELLK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHHH
FCRKFTVPMRQALRGKKMLTERDSNRIPVLHICFIAPGHCYVGYSYTNNNSQFFMGIPRL
HHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCEEEEECCCE
KFPADAPSRSTLKLEEAFHVFIPKEEWDDRLASGMWGVDLGACPGGWTYQLVKRSMFVHA
ECCCCCCCCCEEEEECCEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IDNGMMAQSLMDTGQVKHHMVDGFKFEPPRKNVTWLICDMVEKPARVAHLMGEWLIKGWA
HCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEEHHHHCHHHHHHHHHHHHHHHHH
KEALFNLKLPMKGRYDEVLQDIENLKDFLKKNGFQYKLQAKHLYHDREEITVHIQAISNI
HHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCEEEEEEEECCC
SPH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA