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The map label for this gene is gltX [H]

Identifier: 227828290

GI number: 227828290

Start: 1862857

End: 1864584

Strand: Direct

Name: gltX [H]

Synonym: M1425_2031

Alternate gene names: 227828290

Gene position: 1862857-1864584 (Clockwise)

Preceding gene: 227828289

Following gene: 227828291

Centisome position: 71.41

GC content: 36.69

Gene sequence:

>1728_bases
ATGTCATCAAGTGAACTACGGGAAATTATATATAAATACGCTCTTCAAAACGCGGTAAAGCATGATGGTAAAGCTGAAGC
GGGCCCGGTGATGAGTAAGATCATAGCAGAGAGGCCGGAATTAAGATCTAATGCAAAAGAAATAGTAAAAATTGTCAAGG
AGATTATAGATCAAGTTAATTCACTAACCTTGGAGCAACAAATAGCTGAAATTGAGACTAAGTATCCTGAGTTATTAGAA
GAGAAGAAGCACGAAGAAAAAAAGAAAATATTACCACCCCTTCCTAACGTTAAAGGACAAGTGGTAACTAGATTCGCACC
TAATCCAGACGGTCCTCTTCATTTAGGAAATGCCAGAGCTGCTATCTTATCTTACGAATACGCCAAGATGTATAACGGTA
AATTCATACTGAGGTTTGATGATACGGATCCTAAGGTTAAAAGACCAATTCTAGACGCATATGATTGGATTAAGGAGGAT
CTAAAATGGTTGGGGATTAAATGGGAACAAGAGCTTTACGCTTCAGAGAGACTTGAGGTATACTACAAGTATGCTCGTTA
TCTGATAGAAAAGGGACATGCTTATGTTGATACATGTGATTCTAGTACTTTCAAGAAGTTTAGAGACAGTAGAGGCAAGA
TGAGGGAGCCAGAATGTCTACATAGAAGCTCTTCACCAGAGAGTAACCTAGAACTTTTTGAAAAAATGCTAGAAGGAATG
TTCAAAGAGGGAGAAGCTGTGGTTAGGTTAAAGACCAATTTAATGGATCCGGATCCCTCTCAAATAGACTGGGTAATGCT
TAGGATAATTGATACTACTAAAAATCCTCATCCACGAGTAGGGAGTAAGTATTATGTATGGCCTACCTATAATTTCGCTT
CTATAATAGATGATCATGAACTTGGTGTTACTCATATATTAAGAGCTAAAGAACATATGTCGAATACGGAAAAACAGAGG
TACATTTCTGAGTATATGGGATGGGAATTTCCAGAAGTCTTACAGTTTGGGAGACTAAAACTTGAGGGTTTTATGATGAG
TAAGTCGAAAATAAGAGGAATGCTAGAGAAGGGTGCCAATAGGGATGATCCTAGGCTACCAACTTTGGCTGGACTCAGAA
GAAGGGGAATCTTACCAGATACTATTAAAGATGTGATAATTGATGTTGGAGTAAAGGTAACTGATGCTACTATAAGTTTC
GAAAACATTGCTGCGATTAATAGGAAAAAATTAGATCCAGTAGCTAAAAGAATAATGTTCATAAAAGATGGGGAAGAGTT
TGCCATTGAGCTACCTCAAACGTTGAAAGCTAAGATACCCTTAATTCCTTCTAAACAAGAGGAGGTGGATAGAACTATCA
TTGTTAATTCTGGTGATAAAATTTTGATAGAATCTATCGATGCAGAGGATGGTAGCATATTGAGGTTAATGGAGTTGTGT
AATGTCAGAGTTGATAAGCGTAATCGTAAGCTTGTTTTTCACAGTAAGACGTTAGATGAGGCTAAGAAAGTCAATGCAAG
AATAGTTCAATGGGTAAAATCGGACGAAAAAGTTCCAGTAATAGTAGAAAAAGCGGAAAGAGATGATATAAAAATAATAA
ATGGTTATGCAGAAAGAATTGTAAAGGATTTGGAGATAGATGAAATTGTGCAATTTTATAGATTTGGATTTGTAAGAGTG
GACAAGAAAGACGGTAATATGGTCTCTGTAATCTTTTCACACGATTAA

Upstream 100 bases:

>100_bases
GCGGATGATGAACTGAAAGATATAGCTGATTATATAACAAGTAATAAAAGCGGAAAAGGTGTAAGGGAATTTGTGGATAA
ATTACTAAAAGGGGAGCTTG

Downstream 100 bases:

>100_bases
TCTCCTCCATGTAACCCAAGGAGATTTTTATTATCGATTTAACGTTATACCTATCATTTAAGTGGCACATGAGAGGAACA
TAGATAGTGTCATCATTAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSSSELREIIYKYALQNAVKHDGKAEAGPVMSKIIAERPELRSNAKEIVKIVKEIIDQVNSLTLEQQIAEIETKYPELLE
EKKHEEKKKILPPLPNVKGQVVTRFAPNPDGPLHLGNARAAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKED
LKWLGIKWEQELYASERLEVYYKYARYLIEKGHAYVDTCDSSTFKKFRDSRGKMREPECLHRSSSPESNLELFEKMLEGM
FKEGEAVVRLKTNLMDPDPSQIDWVMLRIIDTTKNPHPRVGSKYYVWPTYNFASIIDDHELGVTHILRAKEHMSNTEKQR
YISEYMGWEFPEVLQFGRLKLEGFMMSKSKIRGMLEKGANRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISF
ENIAAINRKKLDPVAKRIMFIKDGEEFAIELPQTLKAKIPLIPSKQEEVDRTIIVNSGDKILIESIDAEDGSILRLMELC
NVRVDKRNRKLVFHSKTLDEAKKVNARIVQWVKSDEKVPVIVEKAERDDIKIINGYAERIVKDLEIDEIVQFYRFGFVRV
DKKDGNMVSVIFSHD

Sequences:

>Translated_575_residues
MSSSELREIIYKYALQNAVKHDGKAEAGPVMSKIIAERPELRSNAKEIVKIVKEIIDQVNSLTLEQQIAEIETKYPELLE
EKKHEEKKKILPPLPNVKGQVVTRFAPNPDGPLHLGNARAAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKED
LKWLGIKWEQELYASERLEVYYKYARYLIEKGHAYVDTCDSSTFKKFRDSRGKMREPECLHRSSSPESNLELFEKMLEGM
FKEGEAVVRLKTNLMDPDPSQIDWVMLRIIDTTKNPHPRVGSKYYVWPTYNFASIIDDHELGVTHILRAKEHMSNTEKQR
YISEYMGWEFPEVLQFGRLKLEGFMMSKSKIRGMLEKGANRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISF
ENIAAINRKKLDPVAKRIMFIKDGEEFAIELPQTLKAKIPLIPSKQEEVDRTIIVNSGDKILIESIDAEDGSILRLMELC
NVRVDKRNRKLVFHSKTLDEAKKVNARIVQWVKSDEKVPVIVEKAERDDIKIINGYAERIVKDLEIDEIVQFYRFGFVRV
DKKDGNMVSVIFSHD
>Mature_574_residues
SSSELREIIYKYALQNAVKHDGKAEAGPVMSKIIAERPELRSNAKEIVKIVKEIIDQVNSLTLEQQIAEIETKYPELLEE
KKHEEKKKILPPLPNVKGQVVTRFAPNPDGPLHLGNARAAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKEDL
KWLGIKWEQELYASERLEVYYKYARYLIEKGHAYVDTCDSSTFKKFRDSRGKMREPECLHRSSSPESNLELFEKMLEGMF
KEGEAVVRLKTNLMDPDPSQIDWVMLRIIDTTKNPHPRVGSKYYVWPTYNFASIIDDHELGVTHILRAKEHMSNTEKQRY
ISEYMGWEFPEVLQFGRLKLEGFMMSKSKIRGMLEKGANRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISFE
NIAAINRKKLDPVAKRIMFIKDGEEFAIELPQTLKAKIPLIPSKQEEVDRTIIVNSGDKILIESIDAEDGSILRLMELCN
VRVDKRNRKLVFHSKTLDEAKKVNARIVQWVKSDEKVPVIVEKAERDDIKIINGYAERIVKDLEIDEIVQFYRFGFVRVD
KKDGNMVSVIFSHD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=537, Percent_Identity=29.7951582867784, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI4826960, Length=353, Percent_Identity=33.7110481586402, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI134288884, Length=310, Percent_Identity=27.741935483871, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786895, Length=524, Percent_Identity=29.9618320610687, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788743, Length=239, Percent_Identity=25.9414225941423, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI226510925, Length=288, Percent_Identity=27.0833333333333, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17511007, Length=508, Percent_Identity=29.7244094488189, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17543332, Length=378, Percent_Identity=31.4814814814815, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71993394, Length=321, Percent_Identity=27.4143302180685, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324742, Length=452, Percent_Identity=32.5221238938053, Blast_Score=223, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321192, Length=532, Percent_Identity=30.2631578947368, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6324540, Length=317, Percent_Identity=30.2839116719243, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24649466, Length=433, Percent_Identity=30.0230946882217, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI17864488, Length=516, Percent_Identity=26.3565891472868, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24665321, Length=318, Percent_Identity=28.3018867924528, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 66504; Mature: 66373

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSELREIIYKYALQNAVKHDGKAEAGPVMSKIIAERPELRSNAKEIVKIVKEIIDQVN
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHH
SLTLEQQIAEIETKYPELLEEKKHEEKKKILPPLPNVKGQVVTRFAPNPDGPLHLGNARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCHH
AILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKEDLKWLGIKWEQELYASERLEV
HHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YYKYARYLIEKGHAYVDTCDSSTFKKFRDSRGKMREPECLHRSSSPESNLELFEKMLEGM
HHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHH
FKEGEAVVRLKTNLMDPDPSQIDWVMLRIIDTTKNPHPRVGSKYYVWPTYNFASIIDDHE
HHCCCEEEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHCCCC
LGVTHILRAKEHMSNTEKQRYISEYMGWEFPEVLQFGRLKLEGFMMSKSKIRGMLEKGAN
CCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
RDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISFENIAAINRKKLDPVAKRIMF
CCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCHHHHHEEE
IKDGEEFAIELPQTLKAKIPLIPSKQEEVDRTIIVNSGDKILIESIDAEDGSILRLMELC
EECCCHHEEECCHHHHCCCCCCCCCHHHCCCEEEEECCCEEEEEECCCCCCHHHHHHHHH
NVRVDKRNRKLVFHSKTLDEAKKVNARIVQWVKSDEKVPVIVEKAERDDIKIINGYAERI
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHHHHH
VKDLEIDEIVQFYRFGFVRVDKKDGNMVSVIFSHD
HHHCCHHHHHHHHHCCEEEEECCCCCEEEEEEECC
>Mature Secondary Structure 
SSSELREIIYKYALQNAVKHDGKAEAGPVMSKIIAERPELRSNAKEIVKIVKEIIDQVN
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHH
SLTLEQQIAEIETKYPELLEEKKHEEKKKILPPLPNVKGQVVTRFAPNPDGPLHLGNARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCHH
AILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKEDLKWLGIKWEQELYASERLEV
HHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
YYKYARYLIEKGHAYVDTCDSSTFKKFRDSRGKMREPECLHRSSSPESNLELFEKMLEGM
HHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHH
FKEGEAVVRLKTNLMDPDPSQIDWVMLRIIDTTKNPHPRVGSKYYVWPTYNFASIIDDHE
HHCCCEEEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHCCCC
LGVTHILRAKEHMSNTEKQRYISEYMGWEFPEVLQFGRLKLEGFMMSKSKIRGMLEKGAN
CCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
RDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISFENIAAINRKKLDPVAKRIMF
CCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCHHHHHEEE
IKDGEEFAIELPQTLKAKIPLIPSKQEEVDRTIIVNSGDKILIESIDAEDGSILRLMELC
EECCCHHEEECCHHHHCCCCCCCCCHHHCCCEEEEECCCEEEEEECCCCCCHHHHHHHHH
NVRVDKRNRKLVFHSKTLDEAKKVNARIVQWVKSDEKVPVIVEKAERDDIKIINGYAERI
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHHHHH
VKDLEIDEIVQFYRFGFVRVDKKDGNMVSVIFSHD
HHHCCHHHHHHHHHCCEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]