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The map label for this gene is menE [H]

Identifier: 227827998

GI number: 227827998

Start: 1611673

End: 1613055

Strand: Direct

Name: menE [H]

Synonym: M1425_1734

Alternate gene names: 227827998

Gene position: 1611673-1613055 (Clockwise)

Preceding gene: 227827993

Following gene: 227827999

Centisome position: 61.78

GC content: 37.24

Gene sequence:

>1383_bases
GTGATAAAAAAGGAAACTGTGAGTCTAGCAACGTTACTTTATAAGTGGTATAAAGAAACTCCTTCAAAAACCTTTTTAAT
TGATAAAAATCAGTCAATTACTTACGAAAAAGCTGCAAGAGAGGTAGCATACGTTGCTTCCAATATCTCACCTGGAGATA
CTGTAGTTCATGTGATGACTAATTCCCTTCAGTCAGTAATTAACTATTTGGCAATATATTGGGCTGGGGGAAAGGTAGCT
GCGGTTGATCCATTAACTTCAGCAGAAGATTTAAAGTTCATTTTGGATGACTCAAATCCTGATCTAATAATAACCGACGA
GGAAATACATAAGAGAGAATACGATGTTCTCAAATCGTATAAGGTTATAGTCAATAAGAAGGGAAAAGACATTTTTACAA
AACCATACGAGTATAGAGAAGACGAGGTAGGCCTAATTTACTATTATGCTGGAATAGCAGGAAGGACGATGCAAGTATTG
CATAGTGCCAGTAGGGTTGAACTTAATTCTGCAACGCTTTACAATGCGATTAACCTTAAAGAGATTAGGGGAATACTAAC
AGTCCCTATAGCTCACGTCTTAGGAAATAGCGTGTTAGGTGTTACCTTGGAAGCTGGAGGAACGCTATATGTTATGGGTA
AGTTTAACGTCAATGAAGCCATTGAAAGTATAAATAAATATAACATTAACTACCTATCAACTGTTCCAACTGTTTACGAT
TACTTGAATAACGAGGAAAAAAGGAAATTAGATAGTTTAGAATTATGTGTAAGCTCTGCTGCTCCGTTGTTTCCATCAAC
CGTAAACACCTTTAAGAGTAAGTACGGAAAGGATATTGTTCAACAGTATGGTTTTACTGAAGGTTTAATTATAACTTTCC
AGCCCAAGGAACTGGCTGGTGTAATTTCAATAGGAAAACCCTTGCCGGGCGTTGAAATAAAGATAGTAGATAACGAGGGG
AAGGAGAATAATGAAGGGGAGTTATGGGTTAAGGCTCCTTGGCTAATGTTAGGATATAAGGATAGAGAGGAGACAGCTCG
AGCGTTTAATGAGGGATGGCTAAGAACGGGTGATTTAGTTAGGATTGATGAAAAGGGCCTATTGTATTTTAGAGGAATTA
AAAAGAGGATGCTAAAATTTAAGGGATATCCGATATTTCCGAGAGATTTAGAGGAAATCTTGAAGACTCATCCACTGGTG
GAAGACGTGAAAGTTATAGGAGAAGACGCTGGAAATCTAGGACAACAACCTGTTGCCTTGGTTAAGGTAAAGGAGCTCAA
GACTGGGATTGAAGATGAATTACTTAATTATGTAAATTCTAGGGTAGCTTTTTATAAGAGATTGAAGAAAGTTTATGTGG
TAGATAGGATTGAGCGAAATTGA

Upstream 100 bases:

>100_bases
CCCCACCATCAATTCTGCCGTATTTCCCCTCAAGGTCAAACAACGTAATGTGAATGCGTGAAAGCCCAATAATCTTTATC
ATAAGAATTAATAAGATTGT

Downstream 100 bases:

>100_bases
TTCATTAATTAGGGAAGTTGCTGAAAGGTTGCAAAAGAAGGCTGAGGAGTATGGTAAAAAGAAGGAGGATGCTACCTTAG
TCTTAACTGAAGAGAACTTC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MIKKETVSLATLLYKWYKETPSKTFLIDKNQSITYEKAAREVAYVASNISPGDTVVHVMTNSLQSVINYLAIYWAGGKVA
AVDPLTSAEDLKFILDDSNPDLIITDEEIHKREYDVLKSYKVIVNKKGKDIFTKPYEYREDEVGLIYYYAGIAGRTMQVL
HSASRVELNSATLYNAINLKEIRGILTVPIAHVLGNSVLGVTLEAGGTLYVMGKFNVNEAIESINKYNINYLSTVPTVYD
YLNNEEKRKLDSLELCVSSAAPLFPSTVNTFKSKYGKDIVQQYGFTEGLIITFQPKELAGVISIGKPLPGVEIKIVDNEG
KENNEGELWVKAPWLMLGYKDREETARAFNEGWLRTGDLVRIDEKGLLYFRGIKKRMLKFKGYPIFPRDLEEILKTHPLV
EDVKVIGEDAGNLGQQPVALVKVKELKTGIEDELLNYVNSRVAFYKRLKKVYVVDRIERN

Sequences:

>Translated_460_residues
MIKKETVSLATLLYKWYKETPSKTFLIDKNQSITYEKAAREVAYVASNISPGDTVVHVMTNSLQSVINYLAIYWAGGKVA
AVDPLTSAEDLKFILDDSNPDLIITDEEIHKREYDVLKSYKVIVNKKGKDIFTKPYEYREDEVGLIYYYAGIAGRTMQVL
HSASRVELNSATLYNAINLKEIRGILTVPIAHVLGNSVLGVTLEAGGTLYVMGKFNVNEAIESINKYNINYLSTVPTVYD
YLNNEEKRKLDSLELCVSSAAPLFPSTVNTFKSKYGKDIVQQYGFTEGLIITFQPKELAGVISIGKPLPGVEIKIVDNEG
KENNEGELWVKAPWLMLGYKDREETARAFNEGWLRTGDLVRIDEKGLLYFRGIKKRMLKFKGYPIFPRDLEEILKTHPLV
EDVKVIGEDAGNLGQQPVALVKVKELKTGIEDELLNYVNSRVAFYKRLKKVYVVDRIERN
>Mature_460_residues
MIKKETVSLATLLYKWYKETPSKTFLIDKNQSITYEKAAREVAYVASNISPGDTVVHVMTNSLQSVINYLAIYWAGGKVA
AVDPLTSAEDLKFILDDSNPDLIITDEEIHKREYDVLKSYKVIVNKKGKDIFTKPYEYREDEVGLIYYYAGIAGRTMQVL
HSASRVELNSATLYNAINLKEIRGILTVPIAHVLGNSVLGVTLEAGGTLYVMGKFNVNEAIESINKYNINYLSTVPTVYD
YLNNEEKRKLDSLELCVSSAAPLFPSTVNTFKSKYGKDIVQQYGFTEGLIITFQPKELAGVISIGKPLPGVEIKIVDNEG
KENNEGELWVKAPWLMLGYKDREETARAFNEGWLRTGDLVRIDEKGLLYFRGIKKRMLKFKGYPIFPRDLEEILKTHPLV
EDVKVIGEDAGNLGQQPVALVKVKELKTGIEDELLNYVNSRVAFYKRLKKVYVVDRIERN

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=331, Percent_Identity=28.7009063444109, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI187761345, Length=504, Percent_Identity=25.3968253968254, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI187761343, Length=504, Percent_Identity=25.3968253968254, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI42544132, Length=343, Percent_Identity=24.4897959183673, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI38505220, Length=479, Percent_Identity=22.964509394572, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI115511026, Length=344, Percent_Identity=23.8372093023256, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI157311624, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI157311622, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI58082049, Length=340, Percent_Identity=24.4117647058824, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI122937307, Length=423, Percent_Identity=22.9314420803783, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=512, Percent_Identity=24.8046875, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI145693145, Length=347, Percent_Identity=28.2420749279539, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786810, Length=457, Percent_Identity=24.2888402625821, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788595, Length=259, Percent_Identity=25.4826254826255, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790505, Length=473, Percent_Identity=24.3128964059197, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789201, Length=266, Percent_Identity=25.9398496240602, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI221142682, Length=441, Percent_Identity=20.8616780045351, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=469, Percent_Identity=27.0788912579957, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=279, Percent_Identity=32.9749103942652, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=473, Percent_Identity=25.369978858351, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=497, Percent_Identity=24.5472837022133, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=480, Percent_Identity=24.1666666666667, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=468, Percent_Identity=24.7863247863248, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=468, Percent_Identity=24.5726495726496, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=423, Percent_Identity=24.5862884160757, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=334, Percent_Identity=27.8443113772455, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=504, Percent_Identity=22.4206349206349, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=504, Percent_Identity=22.4206349206349, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=504, Percent_Identity=22.4206349206349, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556552, Length=182, Percent_Identity=29.6703296703297, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141359, Length=280, Percent_Identity=25, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=459, Percent_Identity=23.5294117647059, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355181, Length=503, Percent_Identity=26.2425447316103, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859661, Length=347, Percent_Identity=28.8184438040346, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24581924, Length=331, Percent_Identity=26.5861027190332, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=24.0480961923848, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI20130357, Length=347, Percent_Identity=26.2247838616715, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=185, Percent_Identity=34.5945945945946, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648253, Length=199, Percent_Identity=30.6532663316583, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161076582, Length=133, Percent_Identity=37.593984962406, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648255, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356441, Length=195, Percent_Identity=29.2307692307692, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648676, Length=448, Percent_Identity=23.4375, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52025; Mature: 52025

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKETVSLATLLYKWYKETPSKTFLIDKNQSITYEKAAREVAYVASNISPGDTVVHVMT
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
NSLQSVINYLAIYWAGGKVAAVDPLTSAEDLKFILDDSNPDLIITDEEIHKREYDVLKSY
HHHHHHHHHHEEEECCCEEEEECCCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHH
KVIVNKKGKDIFTKPYEYREDEVGLIYYYAGIAGRTMQVLHSASRVELNSATLYNAINLK
HHEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEECCHHEEECCCHH
EIRGILTVPIAHVLGNSVLGVTLEAGGTLYVMGKFNVNEAIESINKYNINYLSTVPTVYD
HHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCHHHHHHHHHHCCCEEEHHHHHHHH
YLNNEEKRKLDSLELCVSSAAPLFPSTVNTFKSKYGKDIVQQYGFTEGLIITFQPKELAG
HHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHH
VISIGKPLPGVEIKIVDNEGKENNEGELWVKAPWLMLGYKDREETARAFNEGWLRTGDLV
HHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCCCEE
RIDEKGLLYFRGIKKRMLKFKGYPIFPRDLEEILKTHPLVEDVKVIGEDAGNLGQQPVAL
EECCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEE
VKVKELKTGIEDELLNYVNSRVAFYKRLKKVYVVDRIERN
EEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKKETVSLATLLYKWYKETPSKTFLIDKNQSITYEKAAREVAYVASNISPGDTVVHVMT
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
NSLQSVINYLAIYWAGGKVAAVDPLTSAEDLKFILDDSNPDLIITDEEIHKREYDVLKSY
HHHHHHHHHHEEEECCCEEEEECCCCCCCCEEEEEECCCCCEEEECHHHHHHHHHHHHHH
KVIVNKKGKDIFTKPYEYREDEVGLIYYYAGIAGRTMQVLHSASRVELNSATLYNAINLK
HHEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEECCHHEEECCCHH
EIRGILTVPIAHVLGNSVLGVTLEAGGTLYVMGKFNVNEAIESINKYNINYLSTVPTVYD
HHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCHHHHHHHHHHCCCEEEHHHHHHHH
YLNNEEKRKLDSLELCVSSAAPLFPSTVNTFKSKYGKDIVQQYGFTEGLIITFQPKELAG
HHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHH
VISIGKPLPGVEIKIVDNEGKENNEGELWVKAPWLMLGYKDREETARAFNEGWLRTGDLV
HHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCCCEE
RIDEKGLLYFRGIKKRMLKFKGYPIFPRDLEEILKTHPLVEDVKVIGEDAGNLGQQPVAL
EECCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEE
VKVKELKTGIEDELLNYVNSRVAFYKRLKKVYVVDRIERN
EEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]