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The map label for this gene is trxA [H]

Identifier: 227827999

GI number: 227827999

Start: 1613042

End: 1613443

Strand: Direct

Name: trxA [H]

Synonym: M1425_1735

Alternate gene names: 227827999

Gene position: 1613042-1613443 (Clockwise)

Preceding gene: 227827998

Following gene: 227828000

Centisome position: 61.83

GC content: 33.58

Gene sequence:

>402_bases
TTGAGCGAAATTGATTCATTAATTAGGGAAGTTGCTGAAAGGTTGCAAAAGAAGGCTGAGGAGTATGGTAAAAAGAAGGA
GGATGCTACCTTAGTCTTAACTGAAGAGAACTTCGATGAAGTAATTAGAAATAATAAGTTAGTTTTAGTAGATTGCTGGG
CAGAATGGTGCGCTCCATGCCACCTTTATGAACCAATTTACAAAAAAGTTGCTGAAAAGTATAAAGGTAAAGCAGTTTTT
GGAAGATTAAATGTTGATGAGAATCAAAAGATTGCAGATAAGTATAGTGTACTTAACATACCTACTACGTTAATTTTCGT
TAATGGTCAATTAGTTGACTCTTTAGTTGGGGCTGTTGATGAAGATACTTTAGAGAGTACTATAAATAAATACTTAGAAT
GA

Upstream 100 bases:

>100_bases
AAGGAGCTCAAGACTGGGATTGAAGATGAATTACTTAATTATGTAAATTCTAGGGTAGCTTTTTATAAGAGATTGAAGAA
AGTTTATGTGGTAGATAGGA

Downstream 100 bases:

>100_bases
TTGAGGTATATGTAAAAAAGAGATTACAGGCTTTTTTGTTAAATGTTAACTTTTCTGAAAAAGGTATAATTGCAATTACT
GGTAAAAATGGTAGTGGGAA

Product: thioredoxin

Products: NA

Alternate protein names: Trx [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSEIDSLIREVAERLQKKAEEYGKKKEDATLVLTEENFDEVIRNNKLVLVDCWAEWCAPCHLYEPIYKKVAEKYKGKAVF
GRLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVDEDTLESTINKYLE

Sequences:

>Translated_133_residues
MSEIDSLIREVAERLQKKAEEYGKKKEDATLVLTEENFDEVIRNNKLVLVDCWAEWCAPCHLYEPIYKKVAEKYKGKAVF
GRLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVDEDTLESTINKYLE
>Mature_132_residues
SEIDSLIREVAERLQKKAEEYGKKKEDATLVLTEENFDEVIRNNKLVLVDCWAEWCAPCHLYEPIYKKVAEKYKGKAVFG
RLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVDEDTLESTINKYLE

Specific function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI21361403, Length=94, Percent_Identity=37.2340425531915, Blast_Score=76, Evalue=8e-15,
Organism=Homo sapiens, GI50592994, Length=89, Percent_Identity=37.0786516853933, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082331, Length=107, Percent_Identity=38.3177570093458, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1788936, Length=99, Percent_Identity=36.3636363636364, Blast_Score=88, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71998175, Length=103, Percent_Identity=40.7766990291262, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71980565, Length=88, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71980562, Length=90, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI115532254, Length=115, Percent_Identity=32.1739130434783, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25144092, Length=106, Percent_Identity=33.9622641509434, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115532252, Length=115, Percent_Identity=32.1739130434783, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556044, Length=80, Percent_Identity=37.5, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI212646193, Length=98, Percent_Identity=31.6326530612245, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32566189, Length=101, Percent_Identity=33.6633663366337, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17569137, Length=101, Percent_Identity=33.6633663366337, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193209454, Length=101, Percent_Identity=33.6633663366337, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554386, Length=83, Percent_Identity=39.7590361445783, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17507705, Length=87, Percent_Identity=32.183908045977, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323072, Length=85, Percent_Identity=43.5294117647059, Blast_Score=74, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321648, Length=76, Percent_Identity=40.7894736842105, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24639826, Length=91, Percent_Identity=31.8681318681319, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24664269, Length=105, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573567, Length=100, Percent_Identity=30, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005746
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR013766
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00085 Thioredoxin [H]

EC number: NA

Molecular weight: Translated: 15244; Mature: 15112

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIDSLIREVAERLQKKAEEYGKKKEDATLVLTEENFDEVIRNNKLVLVDCWAEWCAPC
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCEEEEECHHHHCCCH
HLYEPIYKKVAEKYKGKAVFGRLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVD
HHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHEECCEEEEEECHHHHHHHHCCCC
EDTLESTINKYLE
HHHHHHHHHHHCC
>Mature Secondary Structure 
SEIDSLIREVAERLQKKAEEYGKKKEDATLVLTEENFDEVIRNNKLVLVDCWAEWCAPC
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCEEEEECHHHHCCCH
HLYEPIYKKVAEKYKGKAVFGRLNVDENQKIADKYSVLNIPTTLIFVNGQLVDSLVGAVD
HHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHEECCEEEEEECHHHHHHHHCCCC
EDTLESTINKYLE
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Reduction reactions; participates in dithiol-disulfide exchange reactions [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3294835 [H]