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The map label for this gene is metG [H]

Identifier: 227827775

GI number: 227827775

Start: 1410508

End: 1410864

Strand: Direct

Name: metG [H]

Synonym: M1425_1507

Alternate gene names: 227827775

Gene position: 1410508-1410864 (Clockwise)

Preceding gene: 227827759

Following gene: 227827776

Centisome position: 54.07

GC content: 37.25

Gene sequence:

>357_bases
ATGCTTAAGCTCATGAGCAGTCAAATTACAATAGACGATTTTGCAAAATTAGACCTTAGGGTAGGAATTGTAAGACAAGC
TGAAAGAATTGAAGGAACAAGACTCCTAAAACTCATGGTTGACCTGGGTTCAGAACAAAGGCAAATAATTGCTGGCTTAG
GAGAATATTACACTCCAGATGAGTTATTAAACAAGAGGATAATAATTATAGTCAATTTAAAGCCTAGAGTGATTAGAGGT
TTTGAGAGCCAAGGTATGTTATTAGCTGCCGGATGTAAGGAGGACGAAGCGAAAGGAATAAAACCTAGGATAATAACTGT
AGATGGAGAAGTCCCTCCAGGAACTAAGATATGTTAA

Upstream 100 bases:

>100_bases
CATTACGACTATTTTACATAGCCGATATAAAAAGTTAGCTTACAACGAGTATAAACTTTTACTTTATTATGTAAATCAAA
ATCCTTATAATTCTTCAATT

Downstream 100 bases:

>100_bases
ATCCATTTGAAAAGTTACAGATCCCACCAAAGGCTAATTACACTATCGATACTATGCTAAAAAGGCTGCCAAAGATAGGT
GGTACCACAGTTAGAGATAG

Product: methionyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MLKLMSSQITIDDFAKLDLRVGIVRQAERIEGTRLLKLMVDLGSEQRQIIAGLGEYYTPDELLNKRIIIIVNLKPRVIRG
FESQGMLLAAGCKEDEAKGIKPRIITVDGEVPPGTKIC

Sequences:

>Translated_118_residues
MLKLMSSQITIDDFAKLDLRVGIVRQAERIEGTRLLKLMVDLGSEQRQIIAGLGEYYTPDELLNKRIIIIVNLKPRVIRG
FESQGMLLAAGCKEDEAKGIKPRIITVDGEVPPGTKIC
>Mature_118_residues
MLKLMSSQITIDDFAKLDLRVGIVRQAERIEGTRLLKLMVDLGSEQRQIIAGLGEYYTPDELLNKRIIIIVNLKPRVIRG
FESQGMLLAAGCKEDEAKGIKPRIITVDGEVPPGTKIC

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788432, Length=89, Percent_Identity=43.8202247191011, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=30, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 13135; Mature: 13135

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLMSSQITIDDFAKLDLRVGIVRQAERIEGTRLLKLMVDLGSEQRQIIAGLGEYYTPD
CCCCCCCCCCHHHHHHHHEEEHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCH
ELLNKRIIIIVNLKPRVIRGFESQGMLLAAGCKEDEAKGIKPRIITVDGEVPPGTKIC
HHCCCEEEEEEECCHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLKLMSSQITIDDFAKLDLRVGIVRQAERIEGTRLLKLMVDLGSEQRQIIAGLGEYYTPD
CCCCCCCCCCHHHHHHHHEEEHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCH
ELLNKRIIIIVNLKPRVIRGFESQGMLLAAGCKEDEAKGIKPRIITVDGEVPPGTKIC
HHCCCEEEEEEECCHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9380682; 10360571 [H]