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The map label for this gene is rhtB [H]

Identifier: 227082069

GI number: 227082069

Start: 2027050

End: 2027670

Strand: Direct

Name: rhtB [H]

Synonym: VCM66_1863

Alternate gene names: 227082069

Gene position: 2027050-2027670 (Clockwise)

Preceding gene: 227082068

Following gene: 227082072

Centisome position: 70.08

GC content: 47.5

Gene sequence:

>621_bases
ATGAATACGGCACTTTTGGGACTCTTTATTCCCACCTTCTTTTTTGTTTCGATTACCCCAGGTATGAATATGACCTTGGC
CCTCACTCTGGGCATGAGCGTTGGCTATCGAAGAACACTATGGATGATGGTAGGCGAACTACTGGGTGTGGCTTTGGTCG
CGGTCTCCGCAGTGGTAGGCATTGCGGCGGTCATGCTGCGTTATCCAGATATCTTCACTCTGTTTAAAATCGTGGGGGCA
AGCTATTTGGTGTATTTAGGTGTGCAAATGTGGCGTTCACGGGGCAAGCTGGCGATCAATATCGAGCAAGAAAACACGTA
CCAAGGCAGTGACTGGGGTCTATTAGTACAAGGTTTTGTGACCGCAATTGCTAACCCTAAAGGCTGGGCGTTTATGGTAT
CCCTACTGCCCCCTTTTATTGATCAATCGTTAAGCCTTGCTCCACAACTGACTGTATTGGTTGCCATTATTCTGCTTTCC
GAATTTATCAGCATGAGTTTGTATGCCACGGGTGGAAAAGGGTTAAAGCGCTTATTAAGCCAAGCTCACCATGTCCGTTT
ACTCAATCGGATTGCAGGTTCGCTAATGGCTGGGGTGGGTATCTGGCTCTACTTTAGTTAG

Upstream 100 bases:

>100_bases
CCTCATTCAATTGAAGATTGCGCATTTCCCAATCGGCCTATATTGTGGAGTTGTTGTTAATGGAACTAACACGATAATCC
TCAGGCTAGGGAGAGAATGG

Downstream 100 bases:

>100_bases
CGAATTGGCCATCCGCCCACCGTAACACGGATGGATGGCCAAACACTTACACCGCCCAAGATAATATGGGCCAACCATGC
TGCTCAGCCTGAGCTTTCAA

Product: hypothetical protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNTALLGLFIPTFFFVSITPGMNMTLALTLGMSVGYRRTLWMMVGELLGVALVAVSAVVGIAAVMLRYPDIFTLFKIVGA
SYLVYLGVQMWRSRGKLAINIEQENTYQGSDWGLLVQGFVTAIANPKGWAFMVSLLPPFIDQSLSLAPQLTVLVAIILLS
EFISMSLYATGGKGLKRLLSQAHHVRLLNRIAGSLMAGVGIWLYFS

Sequences:

>Translated_206_residues
MNTALLGLFIPTFFFVSITPGMNMTLALTLGMSVGYRRTLWMMVGELLGVALVAVSAVVGIAAVMLRYPDIFTLFKIVGA
SYLVYLGVQMWRSRGKLAINIEQENTYQGSDWGLLVQGFVTAIANPKGWAFMVSLLPPFIDQSLSLAPQLTVLVAIILLS
EFISMSLYATGGKGLKRLLSQAHHVRLLNRIAGSLMAGVGIWLYFS
>Mature_206_residues
MNTALLGLFIPTFFFVSITPGMNMTLALTLGMSVGYRRTLWMMVGELLGVALVAVSAVVGIAAVMLRYPDIFTLFKIVGA
SYLVYLGVQMWRSRGKLAINIEQENTYQGSDWGLLVQGFVTAIANPKGWAFMVSLLPPFIDQSLSLAPQLTVLVAIILLS
EFISMSLYATGGKGLKRLLSQAHHVRLLNRIAGSLMAGVGIWLYFS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=192, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22482; Mature: 22482

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALLGLFIPTFFFVSITPGMNMTLALTLGMSVGYRRTLWMMVGELLGVALVAVSAVVG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAAVMLRYPDIFTLFKIVGASYLVYLGVQMWRSRGKLAINIEQENTYQGSDWGLLVQGFV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
TAIANPKGWAFMVSLLPPFIDQSLSLAPQLTVLVAIILLSEFISMSLYATGGKGLKRLLS
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAHHVRLLNRIAGSLMAGVGIWLYFS
HHHHHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MNTALLGLFIPTFFFVSITPGMNMTLALTLGMSVGYRRTLWMMVGELLGVALVAVSAVVG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAAVMLRYPDIFTLFKIVGASYLVYLGVQMWRSRGKLAINIEQENTYQGSDWGLLVQGFV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
TAIANPKGWAFMVSLLPPFIDQSLSLAPQLTVLVAIILLSEFISMSLYATGGKGLKRLLS
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QAHHVRLLNRIAGSLMAGVGIWLYFS
HHHHHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]