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The map label for this gene is folD [H]

Identifier: 227082072

GI number: 227082072

Start: 2030542

End: 2031477

Strand: Direct

Name: folD [H]

Synonym: VCM66_1866

Alternate gene names: 227082072

Gene position: 2030542-2031477 (Clockwise)

Preceding gene: 227082069

Following gene: 227082073

Centisome position: 70.2

GC content: 49.79

Gene sequence:

>936_bases
TTGCCTGTGGATGTTATCAAAAATATCTGCGACAATGTGCGCAAAAATTTTCGCTCCAAACCTGTAAGGAAAGTCATGAC
TGCTCAAAATATTGATGGAACGCTGATTTCGCAAACGGTGCGCTCAGAAGTGGCAGCCCGTGTTAAAGCTCGTGTTCAAG
CTGGGTTACGAGCCCCAGGACTGGCGGTTGTGTTGGTGGGCGAAGATCCTGCTTCACAAGTGTATGTCGGCAGTAAACGC
CGTGCCTGTGAAGAAGTCGGTTTTGTCTCCAAATCTTTCGATTTACCCGCCACCGCTAGCGAAGAAGCCTTGCTGTCACT
GGTTGAAGAACTCAATAACGATCCGCAAATCGATGGCATTTTGGTACAACTGCCGTTACCTGCGGGTATGGATACCACCA
AAGTGTTGGAAAGTATTCACCCAGAGAAAGATGTGGATGGCTTCCACCCTTACAACGTCGGTCGCTTGGCGCAGCGCATT
CCGAAACTACGCTCTTGCACACCCAAAGGCATTATTACTCTGCTTGAACGCTACAATATTCCACTACGCGGCAAACACGC
GGTGATTGTGGGAGCTTCCAACATTGTCGGTCGTCCGATGACGTTAGAGCTACTCTTGGCAGGCTGCACCACCACCACTT
GCCATCGCTTTACTCAAGATCTCGAAGGCCATATTCGCCAAGCAGATATCTTGGTGGTCGCGGTCGGTAAACCTAACTTT
ATTCCGGGAGCATGGATTAAAGAAGGTGCAGTTGTGGTCGATGTTGGCATTAACCGCTTAGATACTGGCAAGCTGGTTGG
CGATGTGGAATACGATGTTGCCCGTACGCGTGCAAGTTTTATCACTCCAGTACCCGGTGGTGTAGGACCTATGACCGTCG
CAAGCTTGATTGAAAACACTATGATGGCGTGTGAGCAGTTCCACACTCAAGGTTAG

Upstream 100 bases:

>100_bases
AAACGGGCTAGATCAACGCCTTTGTCTAACTTTCTGTTTCGAATGAGCAATATTTTACCGATCTCATCTCAACTGCCGAA
AAACAGAACAAAGCAAACGT

Downstream 100 bases:

>100_bases
CCACAGTGGCTATCTGCATCGCGGTATTCACACTCACCTTGTGTTAACGCCTTGTGCTAACACCTGAGAAGCAGTAAGAG
GTCAATACCATGCATGGATT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPVDVIKNICDNVRKNFRSKPVRKVMTAQNIDGTLISQTVRSEVAARVKARVQAGLRAPGLAVVLVGEDPASQVYVGSKR
RACEEVGFVSKSFDLPATASEEALLSLVEELNNDPQIDGILVQLPLPAGMDTTKVLESIHPEKDVDGFHPYNVGRLAQRI
PKLRSCTPKGIITLLERYNIPLRGKHAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTQDLEGHIRQADILVVAVGKPNF
IPGAWIKEGAVVVDVGINRLDTGKLVGDVEYDVARTRASFITPVPGGVGPMTVASLIENTMMACEQFHTQG

Sequences:

>Translated_311_residues
MPVDVIKNICDNVRKNFRSKPVRKVMTAQNIDGTLISQTVRSEVAARVKARVQAGLRAPGLAVVLVGEDPASQVYVGSKR
RACEEVGFVSKSFDLPATASEEALLSLVEELNNDPQIDGILVQLPLPAGMDTTKVLESIHPEKDVDGFHPYNVGRLAQRI
PKLRSCTPKGIITLLERYNIPLRGKHAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTQDLEGHIRQADILVVAVGKPNF
IPGAWIKEGAVVVDVGINRLDTGKLVGDVEYDVARTRASFITPVPGGVGPMTVASLIENTMMACEQFHTQG
>Mature_310_residues
PVDVIKNICDNVRKNFRSKPVRKVMTAQNIDGTLISQTVRSEVAARVKARVQAGLRAPGLAVVLVGEDPASQVYVGSKRR
ACEEVGFVSKSFDLPATASEEALLSLVEELNNDPQIDGILVQLPLPAGMDTTKVLESIHPEKDVDGFHPYNVGRLAQRIP
KLRSCTPKGIITLLERYNIPLRGKHAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTQDLEGHIRQADILVVAVGKPNFI
PGAWIKEGAVVVDVGINRLDTGKLVGDVEYDVARTRASFITPVPGGVGPMTVASLIENTMMACEQFHTQG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=295, Percent_Identity=42.3728813559322, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI222418558, Length=308, Percent_Identity=42.2077922077922, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=44.3298969072165, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI36796743, Length=209, Percent_Identity=29.1866028708134, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=71.3780918727915, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=40.1384083044983, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=42.1404682274247, Blast_Score=225, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=40.7665505226481, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=329, Percent_Identity=26.1398176291793, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=47.6351351351351, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=47.6351351351351, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI62472483, Length=301, Percent_Identity=40.8637873754153, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45551871, Length=301, Percent_Identity=40.8637873754153, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=41.7241379310345, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=41.7241379310345, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 33589; Mature: 33458

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVDVIKNICDNVRKNFRSKPVRKVMTAQNIDGTLISQTVRSEVAARVKARVQAGLRAPG
CCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
LAVVLVGEDPASQVYVGSKRRACEEVGFVSKSFDLPATASEEALLSLVEELNNDPQIDGI
EEEEEECCCCCCCEEECCHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
LVQLPLPAGMDTTKVLESIHPEKDVDGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNI
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
PLRGKHAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTQDLEGHIRQADILVVAVGKPNF
CCCCCCEEEEECHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCC
IPGAWIKEGAVVVDVGINRLDTGKLVGDVEYDVARTRASFITPVPGGVGPMTVASLIENT
CCCCHHHCCCEEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MMACEQFHTQG
HHHHHHHHCCC
>Mature Secondary Structure 
PVDVIKNICDNVRKNFRSKPVRKVMTAQNIDGTLISQTVRSEVAARVKARVQAGLRAPG
CHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
LAVVLVGEDPASQVYVGSKRRACEEVGFVSKSFDLPATASEEALLSLVEELNNDPQIDGI
EEEEEECCCCCCCEEECCHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
LVQLPLPAGMDTTKVLESIHPEKDVDGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNI
EEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
PLRGKHAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTQDLEGHIRQADILVVAVGKPNF
CCCCCCEEEEECHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCC
IPGAWIKEGAVVVDVGINRLDTGKLVGDVEYDVARTRASFITPVPGGVGPMTVASLIENT
CCCCHHHCCCEEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MMACEQFHTQG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA