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The map label for this gene is metA [H]

Identifier: 227081764

GI number: 227081764

Start: 1673470

End: 1674429

Strand: Direct

Name: metA [H]

Synonym: VCM66_1551

Alternate gene names: 227081764

Gene position: 1673470-1674429 (Clockwise)

Preceding gene: 227081762

Following gene: 227081765

Centisome position: 57.86

GC content: 48.75

Gene sequence:

>960_bases
ATGCTTAGGGAGAAAGCTGTGCCGATTAGAATCCCCGACCAATTACCGGCGAGTGATGTGCTACGTAACGAAAACATCTT
CGTTATGTCCGAATCGCGTGCTTCGACGCAGGAAATACGCCCACTCAAGGTGCTATTGTTAAACTTAATGCCTAAAAAAA
TTGAGACGGAAACTCAGTTTTTACGCCTCTTGTCCAACTCCCCTTTGCAGGTCGATATCGAGCTGCTGCGCATCGATGAC
AGGCCAAGCAAAAATACTCCAGAAGAGCATCTCAACACCTTCTATCGCCAATTTGAGCTGGTCAAAAATCGCAACTTCGA
TGGGTTAATCATCACGGGCGCGCCACTGGGCTTAGTACAATTTGAGGATGTCGCCTATTGGCAGCATCTACAAAACATCA
TGGCTTGGGCGAAAGCGCATGTCACCTCCACCCTTTACATCTGTTGGGCGGCGCAAGCTGGGCTGAAGCTGCTGTACAAT
TTGCCCAAACGTACGCGTGAAGAGAAATTGTCCGGTGTCTATTACCACGACATCCACAAGCCGTTTCACCCTTTGCTGCG
TGGGTTTGATGATCGCTTTCTCGCTCCGCACTCTCGCTACGCAGATTTTGATGCGGAATTTTTGGCTGAGCATACTGATC
TCGATATTCTGGCAACGTCCGATGTGGCGGGCGTTTATCTCGCAGCGACCAAAGATAAGCGTAATGTGTTTGTGACTGGC
CACCCTGAGTACGATGCTTACACCCTACATGGTGAATATGTGCGTGATCTGGGCGAAGGGCTTAATCCTGCGATCCCCGT
CAATTACTACCCCAATGACAACCCAGATAACAAACCTTGCGCCAGTTGGCGTAGCCACGGGCACTTGCTGTTTGCCAACT
GGCTTAACTACTGCGTTTATCAACAAACACCGTATGATTTGGAAAAATTCAGCGAAGCCAATTTTACCAAAGACGAATAA

Upstream 100 bases:

>100_bases
GTGCCGAGAGATACTAACTTAGATTGAGGTTCGTTGCATTTTGCTATCGACAATAATTTTCGGTTGAGTTATAAGTCTGG
ATGTTTAGACGTCCAAATTA

Downstream 100 bases:

>100_bases
TCCTTCGGGCCGCCCTGTGCGGCCTTTTATCCCTTTACTCGCCTTTCCGCCTTTAGCTTCTGCCTGACATCCCATCCACT
TGGCGTTGCAGCTTAAAGTC

Product: homoserine O-succinyltransferase

Products: NA

Alternate protein names: Homoserine O-transsuccinylase; HTS [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLREKAVPIRIPDQLPASDVLRNENIFVMSESRASTQEIRPLKVLLLNLMPKKIETETQFLRLLSNSPLQVDIELLRIDD
RPSKNTPEEHLNTFYRQFELVKNRNFDGLIITGAPLGLVQFEDVAYWQHLQNIMAWAKAHVTSTLYICWAAQAGLKLLYN
LPKRTREEKLSGVYYHDIHKPFHPLLRGFDDRFLAPHSRYADFDAEFLAEHTDLDILATSDVAGVYLAATKDKRNVFVTG
HPEYDAYTLHGEYVRDLGEGLNPAIPVNYYPNDNPDNKPCASWRSHGHLLFANWLNYCVYQQTPYDLEKFSEANFTKDE

Sequences:

>Translated_319_residues
MLREKAVPIRIPDQLPASDVLRNENIFVMSESRASTQEIRPLKVLLLNLMPKKIETETQFLRLLSNSPLQVDIELLRIDD
RPSKNTPEEHLNTFYRQFELVKNRNFDGLIITGAPLGLVQFEDVAYWQHLQNIMAWAKAHVTSTLYICWAAQAGLKLLYN
LPKRTREEKLSGVYYHDIHKPFHPLLRGFDDRFLAPHSRYADFDAEFLAEHTDLDILATSDVAGVYLAATKDKRNVFVTG
HPEYDAYTLHGEYVRDLGEGLNPAIPVNYYPNDNPDNKPCASWRSHGHLLFANWLNYCVYQQTPYDLEKFSEANFTKDE
>Mature_319_residues
MLREKAVPIRIPDQLPASDVLRNENIFVMSESRASTQEIRPLKVLLLNLMPKKIETETQFLRLLSNSPLQVDIELLRIDD
RPSKNTPEEHLNTFYRQFELVKNRNFDGLIITGAPLGLVQFEDVAYWQHLQNIMAWAKAHVTSTLYICWAAQAGLKLLYN
LPKRTREEKLSGVYYHDIHKPFHPLLRGFDDRFLAPHSRYADFDAEFLAEHTDLDILATSDVAGVYLAATKDKRNVFVTG
HPEYDAYTLHGEYVRDLGEGLNPAIPVNYYPNDNPDNKPCASWRSHGHLLFANWLNYCVYQQTPYDLEKFSEANFTKDE

Specific function: Methionine biosynthesis; HTS variant; first step. [C]

COG id: COG1897

COG function: function code E; Homoserine trans-succinylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HTS family [H]

Homologues:

Organism=Escherichia coli, GI1790443, Length=301, Percent_Identity=66.7774086378738, Blast_Score=426, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005697
- ProDom:   PD037892 [H]

Pfam domain/function: PF04204 HTS [H]

EC number: =2.3.1.46 [H]

Molecular weight: Translated: 36896; Mature: 36896

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLREKAVPIRIPDQLPASDVLRNENIFVMSESRASTQEIRPLKVLLLNLMPKKIETETQF
CCCCCCCCEECCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LRLLSNSPLQVDIELLRIDDRPSKNTPEEHLNTFYRQFELVKNRNFDGLIITGAPLGLVQ
HHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
FEDVAYWQHLQNIMAWAKAHVTSTLYICWAAQAGLKLLYNLPKRTREEKLSGVYYHDIHK
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHCHHHHHHHHHCCCHHHHHCC
PFHPLLRGFDDRFLAPHSRYADFDAEFLAEHTDLDILATSDVAGVYLAATKDKRNVFVTG
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEEEEEECCCCEEEEEC
HPEYDAYTLHGEYVRDLGEGLNPAIPVNYYPNDNPDNKPCASWRSHGHLLFANWLNYCVY
CCCCCEEEECHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEHHHHHHHHEE
QQTPYDLEKFSEANFTKDE
CCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLREKAVPIRIPDQLPASDVLRNENIFVMSESRASTQEIRPLKVLLLNLMPKKIETETQF
CCCCCCCCEECCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LRLLSNSPLQVDIELLRIDDRPSKNTPEEHLNTFYRQFELVKNRNFDGLIITGAPLGLVQ
HHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
FEDVAYWQHLQNIMAWAKAHVTSTLYICWAAQAGLKLLYNLPKRTREEKLSGVYYHDIHK
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHCHHHHHHHHHCCCHHHHHCC
PFHPLLRGFDDRFLAPHSRYADFDAEFLAEHTDLDILATSDVAGVYLAATKDKRNVFVTG
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEEEEEECCCCEEEEEC
HPEYDAYTLHGEYVRDLGEGLNPAIPVNYYPNDNPDNKPCASWRSHGHLLFANWLNYCVY
CCCCCEEEECHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCEEHHHHHHHHEE
QQTPYDLEKFSEANFTKDE
CCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]