Click here to switch to the map view.

The map label for this gene is tolC [C]

Identifier: 227081759

GI number: 227081759

Start: 1668241

End: 1669671

Strand: Direct

Name: tolC [C]

Synonym: VCM66_1546

Alternate gene names: 227081759

Gene position: 1668241-1669671 (Clockwise)

Preceding gene: 227081752

Following gene: 227081760

Centisome position: 57.67

GC content: 48.08

Gene sequence:

>1431_bases
ATGAAAGTGAAATCTCTCTCTCTGGCGATCTGCTCTATATTATTTATGCCGATTGCTCATGCAGCGCCTCTCTCATTTCA
AGAAGCTTGGGACTTGCTGCAACAACAAAACAACTCGCTTGCGGCGCAAAGAGCCAATGTGGAACGTTACGCTCATCTGA
AAGATGCCACACAAAATCTCAATCTACCCTCGGTCAGTGTGGGAGCCAATTTTACGCGGCTTGATCAGGATGTAACACTT
TCCGGCAAGCAGATAGTAGACAGCGCTGGCGGAATAGACCCAAATCTGATTGCTAGCCTATCGCCTAGTCTCTTACCACT
AGTTACTCATCTGGGAGCCGCTAAATCTACCATCACTGAACGCGATATTTTCACTTCCTCGATCCGTGCGATTTGGCCAA
TTTTTACTGGCGGACGAATTACTGCTGCGCAAGATGCCGCCGCGGGTAAAGAAGAGGAAGCGCAAAGTCAGCTCGCGATG
GAAATACAAGCGCGATATGAAGATTTAGCCAAATATTACTTTAGCGTAGTGTTGGCCAAAGAGGTGCTCGCTACCCGAAT
CGCGGTTGAAGAGGGACTCACGCAACACAGAGATAATGCCCTTAAACTTGAGCAACAAGGACAAATTGCTCATGTCGAGC
GCCTGCAAGCGGATGCGTCACTGGATAAAGCCAAAGTTGAGCGTAAAAAAGCACAAAAAACCTTGGACATTGCGCAAAGT
GCGCTAACCCAAATTTTAGGCGCTACAGAAACCGTAGAACCGAGTGGCATGCTGTTCATCAACACCAGCTTACCGCCCAT
GCACGCCTTCATCGAACAAACGCTCAATACCTATCCCGGCTTAAGCCTGCTCGATGCCAAAGAAAAGCAAGCCAGCAGTC
TCATTAAAGCTGAGCAAGGAAAATACTACCCAGAAGTCTATCTGTATGGCGATTATAGCTTGCATGAAGATGACTCACTC
GCTAGCCAAATGAAACCGGATTGGTTGGTTGGCGTTGGTGTGAATATTCCGCTGATTGAAAACAGCGGGCGCAGTGAACA
AGTCAAAGCAGCCAATAGCGCAGTGAATCAAGTGCGCTATTTAAAAGCGCAAGCGAAACAAGATCTCAGTGTGCTGGTGG
AAAAGACCTATCTCGAGGCAGAACAAGCCCTTGAAGAAGTGACGGGACTCAATTCCAGCCTGAACTTGGCACAAGAGAAC
CTGCGTTTACGCCAAAAAGCGTTCAGCCAAGGTTTATCCACCTCCGTCGATGTGGTAGATGCCGAGCTTTATCTGGCCAG
CATTCGCACCCAACAATCGCTAGCGAGCTTTAACTATCTAATTTCACTAAACAAATTATTGGCTCTGTCGAGCGAGATGA
GCAGCTTCTCCACTTACCATCAATCCGCAGTTGCTCTCTCTTCACTGCACCAAAGCGCCACGGCTAAGTAA

Upstream 100 bases:

>100_bases
TTGCAAGTCAGTTTTTTATACTGCGGTCCTTAACTCTTTGTTTGACTTCATCATCCAAAAAATAGAGCATTAGCTCTAAA
TTCATTCTGTCGAGCTCAAA

Downstream 100 bases:

>100_bases
AAGGAATCCAAATGAAAACGGTAAAAACGACCTTAATGACGCTCACCGCTGTCGCTGTTGTTGGCTGGATTGGCTATCGC
TTTTATCAAGCCTATCAACC

Product: hypothetical protein

Products: NA

Alternate protein names: Outer Membrane Protein; Outer Membrane Efflux Family Protein; Outer Membrane Macrolide Efflux Protein; RND Family Drug Transporter; Outer Membrane Efflux Protein Domain Protein; Outer Membrane Protein-Like; Type I Secretion Outer Membrane Protein

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKVKSLSLAICSILFMPIAHAAPLSFQEAWDLLQQQNNSLAAQRANVERYAHLKDATQNLNLPSVSVGANFTRLDQDVTL
SGKQIVDSAGGIDPNLIASLSPSLLPLVTHLGAAKSTITERDIFTSSIRAIWPIFTGGRITAAQDAAAGKEEEAQSQLAM
EIQARYEDLAKYYFSVVLAKEVLATRIAVEEGLTQHRDNALKLEQQGQIAHVERLQADASLDKAKVERKKAQKTLDIAQS
ALTQILGATETVEPSGMLFINTSLPPMHAFIEQTLNTYPGLSLLDAKEKQASSLIKAEQGKYYPEVYLYGDYSLHEDDSL
ASQMKPDWLVGVGVNIPLIENSGRSEQVKAANSAVNQVRYLKAQAKQDLSVLVEKTYLEAEQALEEVTGLNSSLNLAQEN
LRLRQKAFSQGLSTSVDVVDAELYLASIRTQQSLASFNYLISLNKLLALSSEMSSFSTYHQSAVALSSLHQSATAK

Sequences:

>Translated_476_residues
MKVKSLSLAICSILFMPIAHAAPLSFQEAWDLLQQQNNSLAAQRANVERYAHLKDATQNLNLPSVSVGANFTRLDQDVTL
SGKQIVDSAGGIDPNLIASLSPSLLPLVTHLGAAKSTITERDIFTSSIRAIWPIFTGGRITAAQDAAAGKEEEAQSQLAM
EIQARYEDLAKYYFSVVLAKEVLATRIAVEEGLTQHRDNALKLEQQGQIAHVERLQADASLDKAKVERKKAQKTLDIAQS
ALTQILGATETVEPSGMLFINTSLPPMHAFIEQTLNTYPGLSLLDAKEKQASSLIKAEQGKYYPEVYLYGDYSLHEDDSL
ASQMKPDWLVGVGVNIPLIENSGRSEQVKAANSAVNQVRYLKAQAKQDLSVLVEKTYLEAEQALEEVTGLNSSLNLAQEN
LRLRQKAFSQGLSTSVDVVDAELYLASIRTQQSLASFNYLISLNKLLALSSEMSSFSTYHQSAVALSSLHQSATAK
>Mature_476_residues
MKVKSLSLAICSILFMPIAHAAPLSFQEAWDLLQQQNNSLAAQRANVERYAHLKDATQNLNLPSVSVGANFTRLDQDVTL
SGKQIVDSAGGIDPNLIASLSPSLLPLVTHLGAAKSTITERDIFTSSIRAIWPIFTGGRITAAQDAAAGKEEEAQSQLAM
EIQARYEDLAKYYFSVVLAKEVLATRIAVEEGLTQHRDNALKLEQQGQIAHVERLQADASLDKAKVERKKAQKTLDIAQS
ALTQILGATETVEPSGMLFINTSLPPMHAFIEQTLNTYPGLSLLDAKEKQASSLIKAEQGKYYPEVYLYGDYSLHEDDSL
ASQMKPDWLVGVGVNIPLIENSGRSEQVKAANSAVNQVRYLKAQAKQDLSVLVEKTYLEAEQALEEVTGLNSSLNLAQEN
LRLRQKAFSQGLSTSVDVVDAELYLASIRTQQSLASFNYLISLNKLLALSSEMSSFSTYHQSAVALSSLHQSATAK

Specific function: Required For Proper Expression Of Outer Membrane Protein Genes Such As Ompf, Nmpc, Protein 2, Hemolysin, Colicin V, Or Colicin E1. May Be Specialized For Signal Sequence Independent, Extracellular Secretion In Gram-Negative Bacteria. [C]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Outer Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51939; Mature: 51939

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKSLSLAICSILFMPIAHAAPLSFQEAWDLLQQQNNSLAAQRANVERYAHLKDATQNL
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NLPSVSVGANFTRLDQDVTLSGKQIVDSAGGIDPNLIASLSPSLLPLVTHLGAAKSTITE
CCCCEECCCCHHHCCCCCCCCCHHHHHHCCCCCCHHHHHCCCHHHHHHHHHCCHHHHHHH
RDIFTSSIRAIWPIFTGGRITAAQDAAAGKEEEAQSQLAMEIQARYEDLAKYYFSVVLAK
HHHHHHHHHHHHHEECCCEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLATRIAVEEGLTQHRDNALKLEQQGQIAHVERLQADASLDKAKVERKKAQKTLDIAQS
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ALTQILGATETVEPSGMLFINTSLPPMHAFIEQTLNTYPGLSLLDAKEKQASSLIKAEQG
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCC
KYYPEVYLYGDYSLHEDDSLASQMKPDWLVGVGVNIPLIENSGRSEQVKAANSAVNQVRY
CCCCEEEEEECCCCCCCHHHHHHCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHH
LKAQAKQDLSVLVEKTYLEAEQALEEVTGLNSSLNLAQENLRLRQKAFSQGLSTSVDVVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AELYLASIRTQQSLASFNYLISLNKLLALSSEMSSFSTYHQSAVALSSLHQSATAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVKSLSLAICSILFMPIAHAAPLSFQEAWDLLQQQNNSLAAQRANVERYAHLKDATQNL
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NLPSVSVGANFTRLDQDVTLSGKQIVDSAGGIDPNLIASLSPSLLPLVTHLGAAKSTITE
CCCCEECCCCHHHCCCCCCCCCHHHHHHCCCCCCHHHHHCCCHHHHHHHHHCCHHHHHHH
RDIFTSSIRAIWPIFTGGRITAAQDAAAGKEEEAQSQLAMEIQARYEDLAKYYFSVVLAK
HHHHHHHHHHHHHEECCCEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVLATRIAVEEGLTQHRDNALKLEQQGQIAHVERLQADASLDKAKVERKKAQKTLDIAQS
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ALTQILGATETVEPSGMLFINTSLPPMHAFIEQTLNTYPGLSLLDAKEKQASSLIKAEQG
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCC
KYYPEVYLYGDYSLHEDDSLASQMKPDWLVGVGVNIPLIENSGRSEQVKAANSAVNQVRY
CCCCEEEEEECCCCCCCHHHHHHCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHH
LKAQAKQDLSVLVEKTYLEAEQALEEVTGLNSSLNLAQENLRLRQKAFSQGLSTSVDVVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AELYLASIRTQQSLASFNYLISLNKLLALSSEMSSFSTYHQSAVALSSLHQSATAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA