Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 227081752

GI number: 227081752

Start: 1660559

End: 1661620

Strand: Direct

Name: pleD [H]

Synonym: VCM66_1539

Alternate gene names: 227081752

Gene position: 1660559-1661620 (Clockwise)

Preceding gene: 227081751

Following gene: 227081759

Centisome position: 57.41

GC content: 42.66

Gene sequence:

>1062_bases
ATGGATGCTAGGTTATTTGACAATACACAAACGCTTCGAGCTTCAGTGCTATGCGGCCTAAGTTTCTTTTGGGCTTTGAT
CGCTTTCTTGATGGCGCTGATCAATTTCTGGTCAACACGGTTAGTCGAACTCGCGTCACTTGAGCTCGTTTGCGCTTTCT
ACTCCCTGTATATTTATTCTCTGGCTAAGCGTCGTATTCATACCAAACAACAAGTTTATCTTTATTTGTTTATATTAACT
GGCACAACGCTTTTCGCGACCTATATGAAGCCTTTAATGATGGGGGTTTATATTTGGAGCTGCTTCGTTCCCATCCTGTT
TTACATTTTTACCTCTGCTCGCTTTGCCTTTGTTACCTCTTGCCTGTTTTTGATCATACAAAGCAACATTATTTACTGGC
AGTTGCAGCAAACTGGCGCGGCCCTCTCTTGGTCAGTGCTCCTGCATCTTTGCTTTGCTTATATCGGAATTTGGGTGGTT
GCCCATGTTTACGAAGATAACCGACGTAAAATTGAAAACTCCCTACTCTATCTTGCTTCGCGTGATCCTTTAACTGGCGC
ACACAACCGTTTGTCACTCACTACATCATTTCAACATTTTGAACGGTTTAGTGATCAAACCTCACTCTGTTTGCTCGTCA
TTGATTTGGATTTTTTCAAATCGATTAATGATCAATTCGGCCATGACACCGGAGATAAAGTGCTGATTGAAACCACTCGC
CTTTTTACTCAAGTTGTCGGTGACAACAATTTATATCGAATTGGCGGTGAAGAGTTTTGCGTTACTTTATTTGATCAATC
GCTTGAGCAAGCTGGGCGTGTTTGCGAACACTTAAGAGCGATTGTCAGCCAGCATCTGTTCGCGTTTAGAGAGAAACGAG
TACAAGTGACGATCAGTATCGGTGTGTGTGAATATCAAGCAGGAGATCAACTGAACGATCTCCTGAAATTTGCGGATATG
GAATTGTATCGAGCCAAAAAGGCGGGAAGAAATCAGGTTCGCCTATTCAGACATGGCTCAACCAATCCTACTATGGTTAA
TCAACAAACCGAGAACGTGTGA

Upstream 100 bases:

>100_bases
CGCCGTCTACTTTTGTGACATGCATTCAAGATCAATATTGATCAAGATATTCAACTCTGTACTATAAACGCTTACGTTTA
GCCCCGGAGAGGGTGAGTGC

Downstream 100 bases:

>100_bases
GTTTAATTAACGCTGGGATTGCTGGCGTTGTCCTCTTCTTCAAATTCATAAACCACGTTGTAATTCTCCGTGTAGGAACA
GTGTGGTTGTCGACTACAGG

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MDARLFDNTQTLRASVLCGLSFFWALIAFLMALINFWSTRLVELASLELVCAFYSLYIYSLAKRRIHTKQQVYLYLFILT
GTTLFATYMKPLMMGVYIWSCFVPILFYIFTSARFAFVTSCLFLIIQSNIIYWQLQQTGAALSWSVLLHLCFAYIGIWVV
AHVYEDNRRKIENSLLYLASRDPLTGAHNRLSLTTSFQHFERFSDQTSLCLLVIDLDFFKSINDQFGHDTGDKVLIETTR
LFTQVVGDNNLYRIGGEEFCVTLFDQSLEQAGRVCEHLRAIVSQHLFAFREKRVQVTISIGVCEYQAGDQLNDLLKFADM
ELYRAKKAGRNQVRLFRHGSTNPTMVNQQTENV

Sequences:

>Translated_353_residues
MDARLFDNTQTLRASVLCGLSFFWALIAFLMALINFWSTRLVELASLELVCAFYSLYIYSLAKRRIHTKQQVYLYLFILT
GTTLFATYMKPLMMGVYIWSCFVPILFYIFTSARFAFVTSCLFLIIQSNIIYWQLQQTGAALSWSVLLHLCFAYIGIWVV
AHVYEDNRRKIENSLLYLASRDPLTGAHNRLSLTTSFQHFERFSDQTSLCLLVIDLDFFKSINDQFGHDTGDKVLIETTR
LFTQVVGDNNLYRIGGEEFCVTLFDQSLEQAGRVCEHLRAIVSQHLFAFREKRVQVTISIGVCEYQAGDQLNDLLKFADM
ELYRAKKAGRNQVRLFRHGSTNPTMVNQQTENV
>Mature_353_residues
MDARLFDNTQTLRASVLCGLSFFWALIAFLMALINFWSTRLVELASLELVCAFYSLYIYSLAKRRIHTKQQVYLYLFILT
GTTLFATYMKPLMMGVYIWSCFVPILFYIFTSARFAFVTSCLFLIIQSNIIYWQLQQTGAALSWSVLLHLCFAYIGIWVV
AHVYEDNRRKIENSLLYLASRDPLTGAHNRLSLTTSFQHFERFSDQTSLCLLVIDLDFFKSINDQFGHDTGDKVLIETTR
LFTQVVGDNNLYRIGGEEFCVTLFDQSLEQAGRVCEHLRAIVSQHLFAFREKRVQVTISIGVCEYQAGDQLNDLLKFADM
ELYRAKKAGRNQVRLFRHGSTNPTMVNQQTENV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=137, Percent_Identity=39.4160583941606, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786584, Length=329, Percent_Identity=28.8753799392097, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=35, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082007, Length=182, Percent_Identity=33.5164835164835, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=35.1851851851852, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=33.7209302325581, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788956, Length=158, Percent_Identity=35.4430379746835, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=31.6129032258064, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788381, Length=173, Percent_Identity=30.0578034682081, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=154, Percent_Identity=33.1168831168831, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=30.2325581395349, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=29.5302013422819, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40778; Mature: 40778

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARLFDNTQTLRASVLCGLSFFWALIAFLMALINFWSTRLVELASLELVCAFYSLYIYS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKRRIHTKQQVYLYLFILTGTTLFATYMKPLMMGVYIWSCFVPILFYIFTSARFAFVTS
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLFLIIQSNIIYWQLQQTGAALSWSVLLHLCFAYIGIWVVAHVYEDNRRKIENSLLYLAS
HHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
RDPLTGAHNRLSLTTSFQHFERFSDQTSLCLLVIDLDFFKSINDQFGHDTGDKVLIETTR
CCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCCCEEEHHHHH
LFTQVVGDNNLYRIGGEEFCVTLFDQSLEQAGRVCEHLRAIVSQHLFAFREKRVQVTISI
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GVCEYQAGDQLNDLLKFADMELYRAKKAGRNQVRLFRHGSTNPTMVNQQTENV
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCC
>Mature Secondary Structure
MDARLFDNTQTLRASVLCGLSFFWALIAFLMALINFWSTRLVELASLELVCAFYSLYIYS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKRRIHTKQQVYLYLFILTGTTLFATYMKPLMMGVYIWSCFVPILFYIFTSARFAFVTS
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLFLIIQSNIIYWQLQQTGAALSWSVLLHLCFAYIGIWVVAHVYEDNRRKIENSLLYLAS
HHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
RDPLTGAHNRLSLTTSFQHFERFSDQTSLCLLVIDLDFFKSINDQFGHDTGDKVLIETTR
CCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCCCEEEHHHHH
LFTQVVGDNNLYRIGGEEFCVTLFDQSLEQAGRVCEHLRAIVSQHLFAFREKRVQVTISI
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GVCEYQAGDQLNDLLKFADMELYRAKKAGRNQVRLFRHGSTNPTMVNQQTENV
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA