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The map label for this gene is yidA [H]

Identifier: 227081535

GI number: 227081535

Start: 1411086

End: 1411907

Strand: Reverse

Name: yidA [H]

Synonym: VCM66_1319

Alternate gene names: 227081535

Gene position: 1411907-1411086 (Counterclockwise)

Preceding gene: 227081538

Following gene: 227081533

Centisome position: 48.81

GC content: 47.81

Gene sequence:

>822_bases
ATGTACAAACTGATTGCGCTCGATATGGATGGCACACTGCTCAATAGCCAAAAACAGATCTCTCCTCGCACCAAGCAGGC
TATTGCTCAAGCTCGTCAGCAAGGCATTCAAGTGGTTTTGGCTTCTGGCCGCCCTCTTGATGGCATGCGCAGTAAATTGG
AAGAGTTACATCTGACCACGGAAAATGACTATGTACTCTATTACAACGGCTCTATGGTCGCGAATGTCGGTACTGGGGAG
ATTATTCATCAACAAATCATTGATGGCAAAGCGGCTAAGCGTGTGGCAACACTCGCGCAAGAGTTTGGGCTCAATACCCA
TGCCTTTAGCCAAATTCACGGTTTGATCACCCCTAAAACCAGCCAATATACCGAAGTGGAAGCCAGCATAAATGGCCTCA
ATATTACCGAAATGGATTTTGCACAATTGGCAGATGACCACCCGATCATTAAGGCGATGATCGTCGGAGAGCCAGAAAAA
CTCACTCAAGCCATCGCTCAATTACCAGCGCAATGGCGTGAAGAATTTACTGTGGTACAAAGTGCGCCCTTCTTTTTAGA
ATTTCTTAACACGGGCAGTAATAAAGGCATCGGCGTACAAGCGATTGCAGCGCATTTAGGCATTCAAGCCAGTGAAGTGA
TTTGTATGGGAGATGCGGAAAACGATCACCACATGCTGCAATACGCAGGCCTCGGTATCGCAATGGCGAATGCTATGGAA
GAGACCAAACACATTGCGGATCACATTACGCTAAGCAACGATCACGATGGCGTGGCCGTGGCGATAGAACGCTTTGCGCT
AGGCTGTTTCAGCGAAGCTTAA

Upstream 100 bases:

>100_bases
ATTGCTCTAATTTTCATTTTTTATCACTGTGGCTCACAGTTTAAGCACGATGTGTATATAATCGAGCTCGTTCTCTTTTT
CCACTTTATGTAGGTACCCG

Downstream 100 bases:

>100_bases
GTTTTGATGGGGAAGCTTTGGGTAAGGCGTGTTATGCCTTACCTGCTCTCTTTTATCCTCGTCTTTTAACCTTGCTGAGC
CATTTTGAGTACTGTGGCTT

Product: putative phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MYKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTTENDYVLYYNGSMVANVGTGE
IIHQQIIDGKAAKRVATLAQEFGLNTHAFSQIHGLITPKTSQYTEVEASINGLNITEMDFAQLADDHPIIKAMIVGEPEK
LTQAIAQLPAQWREEFTVVQSAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGIAMANAME
ETKHIADHITLSNDHDGVAVAIERFALGCFSEA

Sequences:

>Translated_273_residues
MYKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTTENDYVLYYNGSMVANVGTGE
IIHQQIIDGKAAKRVATLAQEFGLNTHAFSQIHGLITPKTSQYTEVEASINGLNITEMDFAQLADDHPIIKAMIVGEPEK
LTQAIAQLPAQWREEFTVVQSAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGIAMANAME
ETKHIADHITLSNDHDGVAVAIERFALGCFSEA
>Mature_273_residues
MYKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTTENDYVLYYNGSMVANVGTGE
IIHQQIIDGKAAKRVATLAQEFGLNTHAFSQIHGLITPKTSQYTEVEASINGLNITEMDFAQLADDHPIIKAMIVGEPEK
LTQAIAQLPAQWREEFTVVQSAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGIAMANAME
ETKHIADHITLSNDHDGVAVAIERFALGCFSEA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates erythrose 4-phosphate and mannose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=266, Percent_Identity=39.8496240601504, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI87081741, Length=243, Percent_Identity=28.3950617283951, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI48994981, Length=265, Percent_Identity=26.4150943396226, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787043, Length=270, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29712; Mature: 29712

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTT
CEEEEEEECCCCEECCCHHCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHEEEC
ENDYVLYYNGSMVANVGTGEIIHQQIIDGKAAKRVATLAQEFGLNTHAFSQIHGLITPKT
CCCEEEEECCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
SQYTEVEASINGLNITEMDFAQLADDHPIIKAMIVGEPEKLTQAIAQLPAQWREEFTVVQ
CCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGIAMANAME
CCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHCCHHHHHHHHH
ETKHIADHITLSNDHDGVAVAIERFALGCFSEA
HHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKLIALDMDGTLLNSQKQISPRTKQAIAQARQQGIQVVLASGRPLDGMRSKLEELHLTT
CEEEEEEECCCCEECCCHHCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHEEEC
ENDYVLYYNGSMVANVGTGEIIHQQIIDGKAAKRVATLAQEFGLNTHAFSQIHGLITPKT
CCCEEEEECCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
SQYTEVEASINGLNITEMDFAQLADDHPIIKAMIVGEPEKLTQAIAQLPAQWREEFTVVQ
CCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SAPFFLEFLNTGSNKGIGVQAIAAHLGIQASEVICMGDAENDHHMLQYAGLGIAMANAME
CCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHCCHHHHHHHHH
ETKHIADHITLSNDHDGVAVAIERFALGCFSEA
HHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]