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The map label for this gene is prpB

Identifier: 227081507

GI number: 227081507

Start: 1377149

End: 1378075

Strand: Direct

Name: prpB

Synonym: VCM66_1291

Alternate gene names: 227081507

Gene position: 1377149-1378075 (Clockwise)

Preceding gene: 227081506

Following gene: 227081508

Centisome position: 47.61

GC content: 52.32

Gene sequence:

>927_bases
ATGCCCAACAACAAGGTTAAGGAGAATCCCATGAGTTTAAGTCCTGGCGCCAAATTCAGGCTGGCAGTGAAAACCCATCA
TCCACTGCAAATCGTCGGCACCATTAACCCTTACTGCGCGATGATGGCGAAAAGTATCGGCCATCAAGCGATTTATCTCT
CTGGCGGTGGCATTGCCAATGCCTCGTACGGCTTGCCAGATTTGGGCATCACCACACTCAATGACGTGTTGGTAGATGTG
GAACGCATCACCAACGCCTGCGATTTGCCTTTGCTGGTGGATATTGATACCGGCTTTGGCGGCGCATTTAACATTGCGCG
CACCATCAAAGCGATGGAAAAAGCCGGAGCCGCGGCGGTGCACATGGAAGATCAGGTGGCGCAAAAACGCTGTGGTCATC
GTCCGAATAAAGCGATTGTTAGCCAGCAAGAGATGGTGGATCGCGTCAAAGCCGCAGTGGATGCGCGCATCAATCCTGAG
TTTGTGATCATGGCGCGCACCGATGCCTTAGCGGTAGAAGGCATGGACAGTGCGATTGAACGAGCGATCGCCTGTGTTGA
AGCTGGCGCAGACATGATTTTCCCCGAAGCCATGACCGAGCTGAAACAGTACGAACAGTTCTCAACGGCACTGCGTAGTG
CCACAGGTAAACCTGTGCCGATTTTGGCCAACATTACCGAGTTTGGTCAGACTCCCCTTTACAGCGGTGAGCAATTGGCG
GCAGTGAATGTGGATATGGTGCTGTACCCACTCAGCGCTTTCCGTGCCATGAATAAAGCCGCGGAAAATGTGTATCGCCA
CTTGCTGGAACACGGGAATCAGGAAGCGCTGCTCGATCAAATGCAAACGCGCAAAGAGCTTTACGCCTACCTGCATTACC
ACGAATACGAAGACAAGCTGGATCAGCTGTTTTCACAACCCTCTTAA

Upstream 100 bases:

>100_bases
CTGCGTGCGATTCGCCAACGAGATGAAGAGTTGGCCGAAATGCTGATGCGTCGCCACATCTCGCGCAGTCGCCAATTGAT
CGAACAACAAATAATGCTGG

Downstream 100 bases:

>100_bases
GTCGAGGGGAGCCCAAGGAAAACAACCTTCTCTTAGGTTTGACAGCCAATGTGGCTGCGCGAGTAAGAGAACATTAAACC
CAATAACGTGAAATTGCTCT

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPNNKVKENPMSLSPGAKFRLAVKTHHPLQIVGTINPYCAMMAKSIGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDV
ERITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARINPE
FVIMARTDALAVEGMDSAIERAIACVEAGADMIFPEAMTELKQYEQFSTALRSATGKPVPILANITEFGQTPLYSGEQLA
AVNVDMVLYPLSAFRAMNKAAENVYRHLLEHGNQEALLDQMQTRKELYAYLHYHEYEDKLDQLFSQPS

Sequences:

>Translated_308_residues
MPNNKVKENPMSLSPGAKFRLAVKTHHPLQIVGTINPYCAMMAKSIGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDV
ERITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARINPE
FVIMARTDALAVEGMDSAIERAIACVEAGADMIFPEAMTELKQYEQFSTALRSATGKPVPILANITEFGQTPLYSGEQLA
AVNVDMVLYPLSAFRAMNKAAENVYRHLLEHGNQEALLDQMQTRKELYAYLHYHEYEDKLDQLFSQPS
>Mature_307_residues
PNNKVKENPMSLSPGAKFRLAVKTHHPLQIVGTINPYCAMMAKSIGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVE
RITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARINPEF
VIMARTDALAVEGMDSAIERAIACVEAGADMIFPEAMTELKQYEQFSTALRSATGKPVPILANITEFGQTPLYSGEQLAA
VNVDMVLYPLSAFRAMNKAAENVYRHLLEHGNQEALLDQMQTRKELYAYLHYHEYEDKLDQLFSQPS

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=298, Percent_Identity=64.0939597315436, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1790445, Length=172, Percent_Identity=34.3023255813954, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRPB_VIBCH (Q9KSC2)

Other databases:

- EMBL:   AE003852
- PIR:   H82212
- RefSeq:   NP_230980.1
- ProteinModelPortal:   Q9KSC2
- SMR:   Q9KSC2
- GeneID:   2614790
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1336
- TIGR:   VC_1336
- HOGENOM:   HBG728656
- OMA:   GMTSLND
- ProtClustDB:   PRK11320
- BRENDA:   4.1.3.30
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- TIGRFAMs:   TIGR02317

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.30

Molecular weight: Translated: 33737; Mature: 33606

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNKVKENPMSLSPGAKFRLAVKTHHPLQIVGTINPYCAMMAKSIGHQAIYLSGGGIAN
CCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCC
ASYGLPDLGITTLNDVLVDVERITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
HMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARINPEFVIMARTDALAVEGMDSAIE
EHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCHHHHHH
RAIACVEAGADMIFPEAMTELKQYEQFSTALRSATGKPVPILANITEFGQTPLYSGEQLA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCCCCCEEE
AVNVDMVLYPLSAFRAMNKAAENVYRHLLEHGNQEALLDQMQTRKELYAYLHYHEYEDKL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQLFSQPS
HHHHCCCC
>Mature Secondary Structure 
PNNKVKENPMSLSPGAKFRLAVKTHHPLQIVGTINPYCAMMAKSIGHQAIYLSGGGIAN
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCC
ASYGLPDLGITTLNDVLVDVERITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
HMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARINPEFVIMARTDALAVEGMDSAIE
EHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCHHHHHH
RAIACVEAGADMIFPEAMTELKQYEQFSTALRSATGKPVPILANITEFGQTPLYSGEQLA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCCCCCEEE
AVNVDMVLYPLSAFRAMNKAAENVYRHLLEHGNQEALLDQMQTRKELYAYLHYHEYEDKL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQLFSQPS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301