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The map label for this gene is prpC [H]

Identifier: 227081508

GI number: 227081508

Start: 1378251

End: 1379384

Strand: Direct

Name: prpC [H]

Synonym: VCM66_1292

Alternate gene names: 227081508

Gene position: 1378251-1379384 (Clockwise)

Preceding gene: 227081507

Following gene: 227081509

Centisome position: 47.65

GC content: 52.65

Gene sequence:

>1134_bases
ATGTCTGCTGCAAAAGAGTTAGGCGGAGCAGGGCTGCGCGGCCAAAGCGCGGGAAGCACCGCTTTGTGTACTGTGGGAAA
AACGGGGACCGGGTTAACCTACCGTGGTTACGACATTACCGATTTGGCCCATCATGCTCAGTTTGAAGAGGTTGCGCACC
TGCTATTGGTGGGGTATTTGCCAACACAAGCGGAGCTGGATCAGTACAAAACTCGTTTGATTGGCCTGCGCGGTTTACCC
GAAAAACTTAAACAAGCACTGGAATTGATCCCTGCTGAAGCGCATCCGATGGATGTGATGCGCACGGGCTGCTCCATTCT
TGGCAACCTTGAGCCTGAACATACCTTCGCTGAGCAACAAGCGGCGACCGAGCGTATGTTGGCGCTGTTTCCTGCGATTA
TTTGTTACTGGTATCGCTTCAGTCATGAAGGGGTGCGGATTGATACCGCCGATCAAAGTGAAGATTCGATTGGCGGCTAT
TTCCTCAAAATGCTCACTGGCCAAGCGCCGAGCGAGCTGTTCAAAAAAGTGATGCACTGCTCGTTAACTCTGTACGCTGA
GCACGAATTCAATGCTTCTACCTTTGCGGCAAGAGTCTGTGCGTCAACCTTGTCGGATATTCACTCTTGTGTGACAGGCG
CGATTGGTACGCTACGCGGGCCTTTGCATGGCGGAGCGAATGAAGCCGCGATGGCGATGATTGAGCAGTGGCACAGTGCC
GATGAAGCGGAAGCGGGCATTATGCGCATGCTGGCGAGCAAAGAGAAAATCATGGGCTTTGGCCACGCGATTTATCGCGA
GAGTGACCCACGCAACGCCTTAATCAAAGAGTGGTCGAAGGCGCTTTCTGAAGCGGTGGGGGATTCTCATCTCTACGCTG
TTTCTGAGCGTGTGGAAGCGGTGATGAAGCGCGAGAAAGATCTGTTCTGTAATGCGGACTTCTTCCACGCTTCGGCCTAT
CACTTTATGGGCATTCCAACCAAATTGTTCACGCCAATCTTTGTGATGAGCCGTTTAACTGGCTGGGCGGCGCATGTTTA
TGAGCAGCGTGCCAACAACCGAATTATTCGCCCAAGTGCCGATTATGTAGGGCCTGAGCATCAAACTTGGCTGCCGATTG
AACAGCGTGGCTAA

Upstream 100 bases:

>100_bases
AAACCCAATAACGTGAAATTGCTCTCCTAAAACACCTGAAGGTGGACACTAAGAGGTCGGTGCACTAGCACCAAAAGGAG
AGCCAGAAAAGGAGTTCGAC

Downstream 100 bases:

>100_bases
CACTGAACAAGTACAACGAACCCTCCAGCGGAGGGTTCAGAGCCATTTGAATGGCCAGCAGCATCTTTTAGTTCCTAAGC
AATGGAAGCAAAAACATGAA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSAAKELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLAHHAQFEEVAHLLLVGYLPTQAELDQYKTRLIGLRGLP
EKLKQALELIPAEAHPMDVMRTGCSILGNLEPEHTFAEQQAATERMLALFPAIICYWYRFSHEGVRIDTADQSEDSIGGY
FLKMLTGQAPSELFKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTGAIGTLRGPLHGGANEAAMAMIEQWHSA
DEAEAGIMRMLASKEKIMGFGHAIYRESDPRNALIKEWSKALSEAVGDSHLYAVSERVEAVMKREKDLFCNADFFHASAY
HFMGIPTKLFTPIFVMSRLTGWAAHVYEQRANNRIIRPSADYVGPEHQTWLPIEQRG

Sequences:

>Translated_377_residues
MSAAKELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLAHHAQFEEVAHLLLVGYLPTQAELDQYKTRLIGLRGLP
EKLKQALELIPAEAHPMDVMRTGCSILGNLEPEHTFAEQQAATERMLALFPAIICYWYRFSHEGVRIDTADQSEDSIGGY
FLKMLTGQAPSELFKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTGAIGTLRGPLHGGANEAAMAMIEQWHSA
DEAEAGIMRMLASKEKIMGFGHAIYRESDPRNALIKEWSKALSEAVGDSHLYAVSERVEAVMKREKDLFCNADFFHASAY
HFMGIPTKLFTPIFVMSRLTGWAAHVYEQRANNRIIRPSADYVGPEHQTWLPIEQRG
>Mature_376_residues
SAAKELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLAHHAQFEEVAHLLLVGYLPTQAELDQYKTRLIGLRGLPE
KLKQALELIPAEAHPMDVMRTGCSILGNLEPEHTFAEQQAATERMLALFPAIICYWYRFSHEGVRIDTADQSEDSIGGYF
LKMLTGQAPSELFKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTGAIGTLRGPLHGGANEAAMAMIEQWHSAD
EAEAGIMRMLASKEKIMGFGHAIYRESDPRNALIKEWSKALSEAVGDSHLYAVSERVEAVMKREKDLFCNADFFHASAYH
FMGIPTKLFTPIFVMSRLTGWAAHVYEQRANNRIIRPSADYVGPEHQTWLPIEQRG

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=381, Percent_Identity=24.1469816272966, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=52.445652173913, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786939, Length=400, Percent_Identity=31.5, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17555174, Length=350, Percent_Identity=23.7142857142857, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319850, Length=379, Percent_Identity=25.5936675461741, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324328, Length=327, Percent_Identity=24.7706422018349, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24640126, Length=382, Percent_Identity=24.3455497382199, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640124, Length=382, Percent_Identity=24.3455497382199, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356863, Length=361, Percent_Identity=22.7146814404432, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 41613; Mature: 41482

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAKELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLAHHAQFEEVAHLLLVGYL
CCCHHHHCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCC
PTQAELDQYKTRLIGLRGLPEKLKQALELIPAEAHPMDVMRTGCSILGNLEPEHTFAEQQ
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
AATERMLALFPAIICYWYRFSHEGVRIDTADQSEDSIGGYFLKMLTGQAPSELFKKVMHC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
SLTLYAEHEFNASTFAARVCASTLSDIHSCVTGAIGTLRGPLHGGANEAAMAMIEQWHSA
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
DEAEAGIMRMLASKEKIMGFGHAIYRESDPRNALIKEWSKALSEAVGDSHLYAVSERVEA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VMKREKDLFCNADFFHASAYHFMGIPTKLFTPIFVMSRLTGWAAHVYEQRANNRIIRPSA
HHHHHHHCCCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
DYVGPEHQTWLPIEQRG
CCCCCCCCEECCCCCCC
>Mature Secondary Structure 
SAAKELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLAHHAQFEEVAHLLLVGYL
CCHHHHCCCCCCCCCCCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCC
PTQAELDQYKTRLIGLRGLPEKLKQALELIPAEAHPMDVMRTGCSILGNLEPEHTFAEQQ
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
AATERMLALFPAIICYWYRFSHEGVRIDTADQSEDSIGGYFLKMLTGQAPSELFKKVMHC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
SLTLYAEHEFNASTFAARVCASTLSDIHSCVTGAIGTLRGPLHGGANEAAMAMIEQWHSA
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
DEAEAGIMRMLASKEKIMGFGHAIYRESDPRNALIKEWSKALSEAVGDSHLYAVSERVEA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VMKREKDLFCNADFFHASAYHFMGIPTKLFTPIFVMSRLTGWAAHVYEQRANNRIIRPSA
HHHHHHHCCCCCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCC
DYVGPEHQTWLPIEQRG
CCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]