Click here to switch to the map view.

The map label for this gene is opuD [H]

Identifier: 227081451

GI number: 227081451

Start: 1311039

End: 1312661

Strand: Direct

Name: opuD [H]

Synonym: VCM66_1234

Alternate gene names: 227081451

Gene position: 1311039-1312661 (Clockwise)

Preceding gene: 227081449

Following gene: 227081453

Centisome position: 45.33

GC content: 48.37

Gene sequence:

>1623_bases
ATGACTCAGAGAGGATACATGACAAAAGAAATTGATAAGTACAGTATCGATAATACGGACTACACCGTAGGCCAAGATAA
TGTGCAGAAATGGGGGTTTGATGTACACAATCCCGTGTTTGGGATCAGTGCAGGTTTGATTGCTGCATTTTTACTACTGA
CCGTACTCGTGGATGCCCACCAAGCTAAGATTGGGCTTGATACCGTAAAGCAGAGCATTATTGCGAGCTTCGACTGGTTG
TTTATCTGGTCGGGTAACTTATTCCTACTGTTTTGTGTCGGATTGGTCATTTCACCGTTTGGCAAAATTCGGTTAGGTGG
ACAGGCCGCGAAAGCGGATCACTCTTTTCTATCTTGGTTAGCGATGTTGTTTGCTGCAGGGATGGGGATTGGTTTGATGT
TCTGGAGTGTCGCAGAACCTGTGGCCTATTATACGGGCTGGTATGAAACACCACTGGGCGTAGAAGCCAACACTCCTCAA
GCTGCGCAACTGGCGATGGGAGCAACCATGTTCCACTGGGGACTTCACCCGTGGGCCATTTATGGTGTTGTTGCTCTATC
ATTGGCTTTTTTCTGCTACAACAAAGGCTTGCCACTTTCAATGCGCTCGGTTTTTTATCCGTTACTGGGTGATCGCGCTT
GGGGTTGGGCAGGGCATGTGGTCGATATTCTTGCGGTATTAGCCACACTGTTTGGTCTGGCAACGTCTCTCGGTCTTGGG
GCGCAGCAAGCGGCTTCTGGTATCCACCATGTATTTGGTTTTGAATCGGGCATCGGGCTACAAATTGCGGTGATCTGTGT
GGTGACAGGTTTAGCCACATTGTCTGTGGTTCGAGGTATTGATGGCGGGGTGAAAGTCATCTCTAACATCAATATGATTG
TCGCGTTTTTATTGCTCGCACTGGTGGCTGTGATTGGTTATAGCGTCACATTGACTTCGATTCCGACGACTTTAATGGCT
TACCTACAAAACCTAATCCCGCTGAGTAATCCTCATGGTCGTGAAGATGAAGTGTGGTTCCAAGGTTGGACGGTCTTTTA
CTGGGCATGGTGGATTTCATGGTCACCGTTCGTGGGCATGTTCATCGCACGTGTTTCTAAAGGACGTACCATTCGTGAAT
TCATTGTGGCGGTCTTGCTGATCCCAACGTTAGTGACACTGGTGTGGATGTCGGTATTTGGTGGTCTGGCCGTGGATCAA
GTGATTAATGAAATCGGCGTGTTAGGTCAAAATGGCTTAACCGATGTTTCGCTGGCCATGTTCCAGATGTTTGATTCGCT
GGTTTTCGGTAAGGTGCTATCCGTGATTGCCGTGGTGCTGGTTCTGGTGTTTTTTATCACGTCGTCAGATTCGGGTTCAC
TGGTCATTGACAGTATTACCTCAGGCGGCAAATTAGATTCACCAGTATTGCAGCGTGTATTTTGGGCAGTACTCGAAGGC
GCAGTCGCGGTTGCACTGCTATGGATTGGAGGCACGGAAGCGGTACAAGCGCTGCAAGCGGGCGTTATCTCTACAGCCTT
GCCATTTACGGTGATCTTGCTACTGATGTGCGTCAGCTTATGGAAAGGGTTAAAAACCGAACCTGAGTTCATGGCTTCCA
GCAAGCGTCTTCACCAATCTTAA

Upstream 100 bases:

>100_bases
AATCGTGCTTTTAATGAATGGAATTAACAATGAGTACAAAGCAATTTGTGATTAAAAAACCGAAAATGCTTTGTTGTCAT
TGTTGTTGAGCTCGATTTAA

Downstream 100 bases:

>100_bases
TACGCGGTATGCGTTGTCAAAAGGGCGGCGCAAACTAAAATCCGCATCAATCATTTCCTAGCCAAAATGTTCAAAAAAAG
CGCGGAGAGCTCTCCGCGCA

Product: transporter, BCCT family

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MTQRGYMTKEIDKYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGLIAAFLLLTVLVDAHQAKIGLDTVKQSIIASFDWL
FIWSGNLFLLFCVGLVISPFGKIRLGGQAAKADHSFLSWLAMLFAAGMGIGLMFWSVAEPVAYYTGWYETPLGVEANTPQ
AAQLAMGATMFHWGLHPWAIYGVVALSLAFFCYNKGLPLSMRSVFYPLLGDRAWGWAGHVVDILAVLATLFGLATSLGLG
AQQAASGIHHVFGFESGIGLQIAVICVVTGLATLSVVRGIDGGVKVISNINMIVAFLLLALVAVIGYSVTLTSIPTTLMA
YLQNLIPLSNPHGREDEVWFQGWTVFYWAWWISWSPFVGMFIARVSKGRTIREFIVAVLLIPTLVTLVWMSVFGGLAVDQ
VINEIGVLGQNGLTDVSLAMFQMFDSLVFGKVLSVIAVVLVLVFFITSSDSGSLVIDSITSGGKLDSPVLQRVFWAVLEG
AVAVALLWIGGTEAVQALQAGVISTALPFTVILLLMCVSLWKGLKTEPEFMASSKRLHQS

Sequences:

>Translated_540_residues
MTQRGYMTKEIDKYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGLIAAFLLLTVLVDAHQAKIGLDTVKQSIIASFDWL
FIWSGNLFLLFCVGLVISPFGKIRLGGQAAKADHSFLSWLAMLFAAGMGIGLMFWSVAEPVAYYTGWYETPLGVEANTPQ
AAQLAMGATMFHWGLHPWAIYGVVALSLAFFCYNKGLPLSMRSVFYPLLGDRAWGWAGHVVDILAVLATLFGLATSLGLG
AQQAASGIHHVFGFESGIGLQIAVICVVTGLATLSVVRGIDGGVKVISNINMIVAFLLLALVAVIGYSVTLTSIPTTLMA
YLQNLIPLSNPHGREDEVWFQGWTVFYWAWWISWSPFVGMFIARVSKGRTIREFIVAVLLIPTLVTLVWMSVFGGLAVDQ
VINEIGVLGQNGLTDVSLAMFQMFDSLVFGKVLSVIAVVLVLVFFITSSDSGSLVIDSITSGGKLDSPVLQRVFWAVLEG
AVAVALLWIGGTEAVQALQAGVISTALPFTVILLLMCVSLWKGLKTEPEFMASSKRLHQS
>Mature_539_residues
TQRGYMTKEIDKYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGLIAAFLLLTVLVDAHQAKIGLDTVKQSIIASFDWLF
IWSGNLFLLFCVGLVISPFGKIRLGGQAAKADHSFLSWLAMLFAAGMGIGLMFWSVAEPVAYYTGWYETPLGVEANTPQA
AQLAMGATMFHWGLHPWAIYGVVALSLAFFCYNKGLPLSMRSVFYPLLGDRAWGWAGHVVDILAVLATLFGLATSLGLGA
QQAASGIHHVFGFESGIGLQIAVICVVTGLATLSVVRGIDGGVKVISNINMIVAFLLLALVAVIGYSVTLTSIPTTLMAY
LQNLIPLSNPHGREDEVWFQGWTVFYWAWWISWSPFVGMFIARVSKGRTIREFIVAVLLIPTLVTLVWMSVFGGLAVDQV
INEIGVLGQNGLTDVSLAMFQMFDSLVFGKVLSVIAVVLVLVFFITSSDSGSLVIDSITSGGKLDSPVLQRVFWAVLEGA
VAVALLWIGGTEAVQALQAGVISTALPFTVILLLMCVSLWKGLKTEPEFMASSKRLHQS

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=513, Percent_Identity=37.4269005847953, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1788102, Length=455, Percent_Identity=32.0879120879121, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1786224, Length=495, Percent_Identity=27.0707070707071, Blast_Score=171, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 58465; Mature: 58334

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure 
TQRGYMTKEIDKYSIDNTDYTVGQDNVQKWGFDVHNPVFGISAGLIAAFLLLTVLVDAH
CCCCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]