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The map label for this gene is mrdA [H]

Identifier: 227081124

GI number: 227081124

Start: 971636

End: 973552

Strand: Reverse

Name: mrdA [H]

Synonym: VCM66_0906

Alternate gene names: 227081124

Gene position: 973552-971636 (Counterclockwise)

Preceding gene: 227081125

Following gene: 227081123

Centisome position: 33.66

GC content: 49.09

Gene sequence:

>1917_bases
ATGTTACGCCAGCATACCCCTATCCGCGACCATCAAGCCGAAACGCATCTGTTTCGTAATCGTGCCATCGTCTCTTTTGT
CGGGATCTTGATCTGCATGGGTCTGCTGGTGACCAACTTGTACAATATTCAGATCAACCAATATCAAGACTACAAAACGC
GATCCAACGATAACCGCATCAAAGTCGTTCCGATTGCGCCTAACCGCGGGCTGATTTTTGACCGTAACGGTGTGCTTCTG
GCGGAAAACCGTCCGGTATTCAACTTAGAAGTGATCCCTGAAAAAGTGCCAAATATGGAAGAGACCATTGCGCGCTTACA
ACAACTCATTGAGATCAGCCCAGAAAAGCTTGCCGCCTTTGAAAAGGAACGCAAACAGACTCGCCGCTTTAATTCTGTAC
CGCTGCTGACTCAGCTTACCGACGATGAAGTGGCGAAATTTTCCGTTAATCAACATAAATTCCCCGGCGTTTCAGTCACC
GCGAACCTCAAACGCTATTACCCCTATGGCGAAGTACTGACCCACGTGATTGGTTACGTATCGCGGATTAACGATAAAGA
TCTCGAACGCTTGGACAAAGAAGGTAAAAAAGCCAACTACCAAGCCACTCGAGATATCGGCAAACTCGGGATTGAACGCT
ACTACGAAGACTTACTGCACGGTACCGCGGGTTATCAGGAAGTCGAAGTCAACAGCCGTGGCCGTGTCATTCGCACCCTT
AAATACGTCCCCCCCATTCCGGGAAAAGATATCGTGCTCAATCTCGATATCGAACTTCAGCTCTATGCCTACAAGCTGCT
CGAAGGTCGCCGCGGCTCGATTGTCGCTCTCGATCCTAAAGATAATGGCGTGCTCGCCATGGCATCGAGCCCAAGTTACG
ATCCCAATGCATTCGTGCATGGTATTTCAGGTAAAGCCTACAGCGATTTACTCAATGACAAACGTCGTCCATTAGTAAAC
CGCACCACGCTTGGCATTTATCCGCCGGGGTCAACCGTCAAACCGTTTATTGCGGTGTCCGCACTGCAAGATGGGATCAT
TACACCCAATACCACCCGTAATGATCCGGGTTACTGGCGCATTCCCAATACCGATACTCGGCCTTTCCGCGACTGGCTAC
GTTGGGGCCACGGCCGGGTAGACATCATTAAATCTCTCGAAGAATCGGTAGATACCTTCTTCTATCAAATCGCCTATGAC
ATGGGGATAGATAAACTCTCCAGTTGGATGATGCGCTTTGGCTTTGGGGATCTGACGGGGATTGATATTTACGAAGAGAG
TAAAGCTAATATGCCAACACGCGAGTGGAAAATGGCACGTCACAAAGTGCCTTGGTATCAAGGTGACACCATTCCGGTTG
GGATTGGACAAGGCTATTGGACTGCCACACCGATGCAAATTGCTAAAGCGACGTCGGTTTTAGTTAACCACGGTAAAGTG
ATTGCCCCGCATCTGCTGCGTGCCACCATAGATAACGGTGAACAGTTCTCAACCCAAAAAGAGACCGAGTACACCACTTA
CCCACCGATTGATGGCGTGCCGAAAAAGTATTGGGACATGGCTATCCATGGCATGTATCTGGTTAACCATGGTGCTAAAG
GAACCGCACGTCGCGCCTTCCAGAATATGCCTTACGAAAGTGCGGGAAAATCAGGGACAGCGCAAGTATTTGGTCTGGCG
GAAGGCCAAAAATATAACGCAAGTGAGTTAGCAGAGCACTTACATGACCATGCTCTGTTTACGGGCTTTGCCCCCGTCAA
AGATCCCAAAGTCATCGTCACCTTCGTATTGGAAAATGGCGGTGGCGGCTCCAGTAATGGTGCGCCAGTCGTGCGACAAA
TTTTCGATCACGTGATTTTGGGCAAAAAAGAAGAAGAGCCAAAAGCAGAATCAAAAGAAGAGGTAATACAGCCATGA

Upstream 100 bases:

>100_bases
GACCTTGCCTCATCCACTAGTGCGCGTGGTGATGGCAGAAAGTTTATACCGTGCGTGGAGCATTACTACTAACCACCCTT
ACCACCGAGAATAGAGTGAC

Downstream 100 bases:

>100_bases
AGATGGATCCTGCAACCGGACAAAACCGGGCGCTGTTTGAGCGTTTTCATATTGATCTGCCTCTACTGCTTGGCGTTCTT
GCCCTAATGGGATTTGGCTT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MLRQHTPIRDHQAETHLFRNRAIVSFVGILICMGLLVTNLYNIQINQYQDYKTRSNDNRIKVVPIAPNRGLIFDRNGVLL
AENRPVFNLEVIPEKVPNMEETIARLQQLIEISPEKLAAFEKERKQTRRFNSVPLLTQLTDDEVAKFSVNQHKFPGVSVT
ANLKRYYPYGEVLTHVIGYVSRINDKDLERLDKEGKKANYQATRDIGKLGIERYYEDLLHGTAGYQEVEVNSRGRVIRTL
KYVPPIPGKDIVLNLDIELQLYAYKLLEGRRGSIVALDPKDNGVLAMASSPSYDPNAFVHGISGKAYSDLLNDKRRPLVN
RTTLGIYPPGSTVKPFIAVSALQDGIITPNTTRNDPGYWRIPNTDTRPFRDWLRWGHGRVDIIKSLEESVDTFFYQIAYD
MGIDKLSSWMMRFGFGDLTGIDIYEESKANMPTREWKMARHKVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNHGKV
IAPHLLRATIDNGEQFSTQKETEYTTYPPIDGVPKKYWDMAIHGMYLVNHGAKGTARRAFQNMPYESAGKSGTAQVFGLA
EGQKYNASELAEHLHDHALFTGFAPVKDPKVIVTFVLENGGGGSSNGAPVVRQIFDHVILGKKEEEPKAESKEEVIQP

Sequences:

>Translated_638_residues
MLRQHTPIRDHQAETHLFRNRAIVSFVGILICMGLLVTNLYNIQINQYQDYKTRSNDNRIKVVPIAPNRGLIFDRNGVLL
AENRPVFNLEVIPEKVPNMEETIARLQQLIEISPEKLAAFEKERKQTRRFNSVPLLTQLTDDEVAKFSVNQHKFPGVSVT
ANLKRYYPYGEVLTHVIGYVSRINDKDLERLDKEGKKANYQATRDIGKLGIERYYEDLLHGTAGYQEVEVNSRGRVIRTL
KYVPPIPGKDIVLNLDIELQLYAYKLLEGRRGSIVALDPKDNGVLAMASSPSYDPNAFVHGISGKAYSDLLNDKRRPLVN
RTTLGIYPPGSTVKPFIAVSALQDGIITPNTTRNDPGYWRIPNTDTRPFRDWLRWGHGRVDIIKSLEESVDTFFYQIAYD
MGIDKLSSWMMRFGFGDLTGIDIYEESKANMPTREWKMARHKVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNHGKV
IAPHLLRATIDNGEQFSTQKETEYTTYPPIDGVPKKYWDMAIHGMYLVNHGAKGTARRAFQNMPYESAGKSGTAQVFGLA
EGQKYNASELAEHLHDHALFTGFAPVKDPKVIVTFVLENGGGGSSNGAPVVRQIFDHVILGKKEEEPKAESKEEVIQP
>Mature_638_residues
MLRQHTPIRDHQAETHLFRNRAIVSFVGILICMGLLVTNLYNIQINQYQDYKTRSNDNRIKVVPIAPNRGLIFDRNGVLL
AENRPVFNLEVIPEKVPNMEETIARLQQLIEISPEKLAAFEKERKQTRRFNSVPLLTQLTDDEVAKFSVNQHKFPGVSVT
ANLKRYYPYGEVLTHVIGYVSRINDKDLERLDKEGKKANYQATRDIGKLGIERYYEDLLHGTAGYQEVEVNSRGRVIRTL
KYVPPIPGKDIVLNLDIELQLYAYKLLEGRRGSIVALDPKDNGVLAMASSPSYDPNAFVHGISGKAYSDLLNDKRRPLVN
RTTLGIYPPGSTVKPFIAVSALQDGIITPNTTRNDPGYWRIPNTDTRPFRDWLRWGHGRVDIIKSLEESVDTFFYQIAYD
MGIDKLSSWMMRFGFGDLTGIDIYEESKANMPTREWKMARHKVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNHGKV
IAPHLLRATIDNGEQFSTQKETEYTTYPPIDGVPKKYWDMAIHGMYLVNHGAKGTARRAFQNMPYESAGKSGTAQVFGLA
EGQKYNASELAEHLHDHALFTGFAPVKDPKVIVTFVLENGGGGSSNGAPVVRQIFDHVILGKKEEEPKAESKEEVIQP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=630, Percent_Identity=56.6666666666667, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=615, Percent_Identity=24.0650406504065, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 72016; Mature: 72016

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQHTPIRDHQAETHLFRNRAIVSFVGILICMGLLVTNLYNIQINQYQDYKTRSNDNRI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEE
KVVPIAPNRGLIFDRNGVLLAENRPVFNLEVIPEKVPNMEETIARLQQLIEISPEKLAAF
EEEEECCCCCEEECCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHH
EKERKQTRRFNSVPLLTQLTDDEVAKFSVNQHKFPGVSVTANLKRYYPYGEVLTHVIGYV
HHHHHHHHHCCCCCEEEECCCCHHHEEECCCCCCCCEEEEECHHHHCCHHHHHHHHHHHH
SRINDKDLERLDKEGKKANYQATRDIGKLGIERYYEDLLHGTAGYQEVEVNSRGRVIRTL
HHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEE
KYVPPIPGKDIVLNLDIELQLYAYKLLEGRRGSIVALDPKDNGVLAMASSPSYDPNAFVH
ECCCCCCCCCEEEEEEEEEEEEEEHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCEEE
GISGKAYSDLLNDKRRPLVNRTTLGIYPPGSTVKPFIAVSALQDGIITPNTTRNDPGYWR
CCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEE
IPNTDTRPFRDWLRWGHGRVDIIKSLEESVDTFFYQIAYDMGIDKLSSWMMRFGFGDLTG
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
IDIYEESKANMPTREWKMARHKVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNHGKV
CEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
IAPHLLRATIDNGEQFSTQKETEYTTYPPIDGVPKKYWDMAIHGMYLVNHGAKGTARRAF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEECCCCHHHHHHH
QNMPYESAGKSGTAQVFGLAEGQKYNASELAEHLHDHALFTGFAPVKDPKVIVTFVLENG
HCCCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECC
GGGSSNGAPVVRQIFDHVILGKKEEEPKAESKEEVIQP
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MLRQHTPIRDHQAETHLFRNRAIVSFVGILICMGLLVTNLYNIQINQYQDYKTRSNDNRI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCEE
KVVPIAPNRGLIFDRNGVLLAENRPVFNLEVIPEKVPNMEETIARLQQLIEISPEKLAAF
EEEEECCCCCEEECCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHH
EKERKQTRRFNSVPLLTQLTDDEVAKFSVNQHKFPGVSVTANLKRYYPYGEVLTHVIGYV
HHHHHHHHHCCCCCEEEECCCCHHHEEECCCCCCCCEEEEECHHHHCCHHHHHHHHHHHH
SRINDKDLERLDKEGKKANYQATRDIGKLGIERYYEDLLHGTAGYQEVEVNSRGRVIRTL
HHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEE
KYVPPIPGKDIVLNLDIELQLYAYKLLEGRRGSIVALDPKDNGVLAMASSPSYDPNAFVH
ECCCCCCCCCEEEEEEEEEEEEEEHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCEEE
GISGKAYSDLLNDKRRPLVNRTTLGIYPPGSTVKPFIAVSALQDGIITPNTTRNDPGYWR
CCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEE
IPNTDTRPFRDWLRWGHGRVDIIKSLEESVDTFFYQIAYDMGIDKLSSWMMRFGFGDLTG
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
IDIYEESKANMPTREWKMARHKVPWYQGDTIPVGIGQGYWTATPMQIAKATSVLVNHGKV
CEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
IAPHLLRATIDNGEQFSTQKETEYTTYPPIDGVPKKYWDMAIHGMYLVNHGAKGTARRAF
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEECCCCHHHHHHH
QNMPYESAGKSGTAQVFGLAEGQKYNASELAEHLHDHALFTGFAPVKDPKVIVTFVLENG
HCCCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECC
GGGSSNGAPVVRQIFDHVILGKKEEEPKAESKEEVIQP
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]